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15 条记录 (耗时 0.026 秒)

    Methods for fragmentation and analysis of nucleic acid
    1.
    发明申请
    Methods for fragmentation and analysis of nucleic acid 审中-公开
    核酸分离和分析方法

    公开(公告)号：US20070218478A1

    公开(公告)日：2007-09-20

    申请号：US11612454

    申请日：2006-12-18

    申请人： Qing Bai ,  Susana Salceda ,  Kai Wu ,  Thong Nguyen ,  Charles Miyada

    发明人： Qing Bai ,  Susana Salceda ,  Kai Wu ,  Thong Nguyen ,  Charles Miyada

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6806 ,  C12Q2521/531 ,  C12Q2521/301 ,  C12Q2521/131

    摘要： Methods for fragmenting and labeling DNA in a single reaction volume and incubation step using a uracil DNA glycosylase, an apurinic/apyrimidinic endonuclease, and a terminal transferase are disclosed. In a preferred embodiment the UDG, AP and TdT activities are first mixed together to form an enzyme mixture and then the enzyme mixture is mixed with the uracil containing DNA. The fragmentation and labeling reactions thus take place simultaneously as part of the same reaction. The methods may be used in a variety of applications where fragmenting and end-labeling single or double stranded DNA is desired.

    摘要翻译： 公开了在单个反应体积中分离和标记DNA的方法以及使用尿嘧啶DNA糖基化酶，无嘌呤/脱嘧啶核苷酸内切酶和末端转移酶的温育步骤。 在优选的实施方案中，首先将UDG，AP和TdT活性混合在一起以形成酶混合物，然后将该酶混合物与含有尿嘧啶的DNA混合。 因此，碎裂和标记反应同时作为相同反应的一部分进行。 该方法可用于需要片段化和末端标记单链或双链DNA的多种应用。

    Methods for fragmenting nucleic acid
    2.
    发明申请
    Methods for fragmenting nucleic acid 审中-公开
    破碎核酸的方法

    公开(公告)号：US20060141498A1

    公开(公告)日：2006-06-29

    申请号：US11273964

    申请日：2005-11-14

    申请人： Kai Wu ,  Charles Miyada ,  Thong Nguyen

    发明人： Kai Wu ,  Charles Miyada ,  Thong Nguyen

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N9/22

    CPC分类号： C12N9/88 ,  C12Q1/6806 ,  C12Q2600/158 ,  C12Q2525/143 ,  C12Q2521/301 ,  C12Q2521/131

    摘要： Methods for using an apurinic/apyrimidinic endonuclease, capable of cleaving both single- and double-stranded cDNA, for fragmentation and labeling of single stranded or double stranded DNA molecules are provided. Amplification methods that generate single-stranded amplified cDNA are also disclosed. In the subject methods AP sites in a population of nucleic acids are cleaved by an AP endonuclease that is active on both double and single stranded DNA. Fragments may be end labeled. In preferred embodiments APE 1 is used. The methods may be used in a variety of applications where end-labeling single or double stranded DNA is desired.

    摘要翻译： 提供了使用能够切割单链和双链cDNA，用于单链或双链DNA分子的片段化和标记的无嘌呤/脱嘧啶核苷酸内切酶的方法。 还公开了产生单链扩增cDNA的扩增方法。 在本发明方法中，核酸群体中的AP位点被双链和单链DNA上活性的AP内切核酸酶切割。 片段可能是末端标记的。 在优选实施方案中，使用APE 1。 所述方法可用于需要终止标记单链或双链DNA的多种应用。

    Method and apparatus for determining a switching signal state
    4.
    发明申请
    Method and apparatus for determining a switching signal state 有权
    用于确定开关信号状态的方法和装置

    公开(公告)号：US20050073202A1

    公开(公告)日：2005-04-07

    申请号：US10663046

    申请日：2003-09-15

    申请人： Michael Gasperi ,  David Brandt ,  Thong Nguyen

    发明人： Michael Gasperi ,  David Brandt ,  Thong Nguyen

    IPC分类号： G01D5/24 ,  H03K17/945 ,  H02B1/24

    CPC分类号： H03K17/955 ,  G01D5/24 ,  H03K2217/960705 ,  Y10T307/766 ,  Y10T307/826

    摘要： A technique for controlling a switching circuit, such as a relay, includes one or more sensing circuits that generate signals based upon the presence of an actuating object and upon a randomly applied strobe signal. The generated signals are sampled and are used as a basis for determining the state of an output signal. The sensing circuit may generate the signals based upon capacitive coupling with the actuating object. The randomization of the sampling provides enhanced immunity to periodic or cyclic noise. Where more than one sensing circuit is included, the output of the circuits may be considered together for determining the state of the output signal, such as based upon predetermined ranges of signal levels. Signals of the sensing circuit may be sampled in the absence of the strobe to provide an indication of the relative noise level. If the noise level is determined to be elevated, the output signal may not change states.

    摘要翻译： 用于控制诸如继电器的开关电路的技术包括一个或多个感测电路，其基于执行对象的存在以及随机应用的选通信号产生信号。 产生的信号被采样，并被用作确定输出信号的状态的基础。 感测电路可以基于与致动对象的电容耦合来产生信号。 采样的随机化提供了对周期性或循环噪声的增强的抗扰性。 在包括多于一个感测电路的情况下，可以一起考虑电路的输出以确定输出信号的状态，例如基于预定的信号电平范围。 感测电路的信号可以在没有选通信号的情况下进行采样，以提供相对噪声电平的指示。 如果确定噪声电平升高，则输出信号可能不会改变状态。

    COMPOSITIONS FOR REPELLING FLUID AND USES THEREOF
    6.
    发明申请
    COMPOSITIONS FOR REPELLING FLUID AND USES THEREOF 有权
    用于回收流体的组合物及其用途

    公开(公告)号：US20110152398A1

    公开(公告)日：2011-06-23

    申请号：US12641606

    申请日：2009-12-18

    申请人： Kofi A. Bissah ,  Ricardo De Oliveira ,  Saurabh Desai ,  Joseph J. LiBrizzi ,  Saroja Narasimhan ,  Thong Nguyen ,  Shoba Pillai

    发明人： Kofi A. Bissah ,  Ricardo De Oliveira ,  Saurabh Desai ,  Joseph J. LiBrizzi ,  Saroja Narasimhan ,  Thong Nguyen ,  Shoba Pillai

    IPC分类号： A61K8/89

    CPC分类号： A61L15/52 ,  A61K8/25 ,  A61K8/37 ,  A61K8/375 ,  A61K8/585 ,  A61K8/895 ,  A61L15/18 ,  A61L15/26 ,  A61Q15/00 ,  A61Q19/005 ,  C08L83/04

    摘要： Provided are compositions for repelling fluids comprising a volatile liquid carrier, a powder-feel agent and less than 5 weight % of an ester selected from the group consisting of formula I, formula II, formula III, and combinations of two or more thereof: wherein R1, R2, R3, R5, R6, R8 and R9 are independently linear or branched, substituted or unsubstituted, saturated or unsaturated, C3-C22 alkyl or alkenyl groups, R4 is a linear or branched, substituted or unsubstituted, saturated or unsaturated, C3-C22 alkylene or alkenylene moiety, and R7 is a linear or branched, substituted or unsubstituted, saturated or unsaturated C3-C22 moiety, the composition being substantially anhydrous. Also provided are methods of improving dryness and/or comfort associated with the intimate area, as well as, kits comprising the composition and an absorbent article.

    摘要翻译： 提供了用于排斥流体的组合物，其包含挥发性液体载体，粉末感受剂和小于5重量％的选自式I，式II，式III的酯，以及其两种或多种的组合：其中 R 1，R 2，R 3，R 5，R 6，R 8和R 9独立地是直链或支链的，取代或未取代的饱和或不饱和的C 3 -C 22烷基或链烯基，R 4是直链或支链的，取代或未取代的饱和或不饱和的， C3-C22亚烷基或亚烯基部分，并且R7是直链或支链的取代或未取代的饱和或不饱和的C 3 -C 22部分，该组合物基本上是无水的。 还提供了改善与紧密区域相关的干燥和/或舒适性的方法，以及包含该组合物和吸收制品的试剂盒。

    PRODUCT DISPENSER AND ABSORBENT ARTICLE KIT
    8.
    发明申请
    PRODUCT DISPENSER AND ABSORBENT ARTICLE KIT 审中-公开
    产品分配器和吸收物品套件

    公开(公告)号：US20110150558A1

    公开(公告)日：2011-06-23

    申请号：US12710468

    申请日：2010-02-23

    申请人： Kofi A. Bissah ,  Ricardo De Oliveira ,  Saurabh Desai ,  Paul Y. Fung ,  Joseph J. LiBrizzi ,  Thong Nguyen ,  Shoba Pillai

    发明人： Kofi A. Bissah ,  Ricardo De Oliveira ,  Saurabh Desai ,  Paul Y. Fung ,  Joseph J. LiBrizzi ,  Thong Nguyen ,  Shoba Pillai

    IPC分类号： B05C11/00 ,  A61F13/15

    CPC分类号： A61F15/001 ,  A45D34/04 ,  A45D34/06 ,  A45D2034/002 ,  A45D2200/1018

    摘要： A kit including a product dispenser assembly and an absorbent article, the assembly including a container housing having a head portion and an internal chamber for holding a composition, the head portion including a plurality of apertures, a moveable member structured to enable a user to selectively deploy the composition from within the internal chamber through the plurality of apertures, a cap assembly, a first absorbent pad arranged on top of the head portion for receiving the composition as it is deployed from the container, a plurality of absorbent pads arranged in a stacked configuration within cap assembly, each of the plurality of pads being adapted to be sequentially arranged on top of the head portion after the first removable pad has used to apply the composition and then removed by the user.

    摘要翻译： 一种包括产品分配器组件和吸收制品的套件，所述组件包括具有头部和用于保持组合物的内部室的容器壳体，所述头部部分包括多个孔，构造成使得使用者能够选择性地 通过所述多个孔从所述内部腔室内部署所述组合物，盖组件，布置在所述头部的顶部上的第一吸收垫，用于在从所述容器展开所述组合物时接收所述组合物;多个吸收垫，被布置成堆叠 在帽组件内的配置中，在第一可移除垫用于施加组合物然后由用户去除之后，多个垫中的每一个适于依次布置在头部的顶部上。

    COMMON CARRIER
    9.
    发明申请
    COMMON CARRIER 有权
    通用卡车

    公开(公告)号：US20070226972A1

    公开(公告)日：2007-10-04

    申请号：US11753874

    申请日：2007-05-25

    申请人： Robert Baan ,  Harry Cox ,  John Gauci ,  John Lankard ,  David Long ,  Thong Nguyen

    发明人： Robert Baan ,  Harry Cox ,  John Gauci ,  John Lankard ,  David Long ,  Thong Nguyen

    IPC分类号： H01L21/027

    CPC分类号： B41F15/36 ,  H05K1/0306 ,  H05K3/1225 ,  H05K2203/0169 ,  H05K2203/0278 ,  Y10T29/41

    摘要： A method for mounting a film, used to fabricate a mask for use in screening an electronic device, to a common carrier frame. The common carrier frame has an outer edge along an outer periphery and an opening in a central portion of the frame. The method includes applying external pressure to the frame outer edge to compress the frame inward and reduce the opening, securing a film to the frame, the film covering the frame opening, and releasing the pressure on the frame to expand the opening and place the film in tension.

    摘要翻译： 用于将用于制造用于屏蔽电子设备的掩模的膜安装到公共载体框架的方法。 公共载架框架具有沿着外周的外边缘和在框架的中心部分中的开口。 该方法包括对框架外边缘施加外部压力以将框架向内压缩并减小开口，将薄膜固定在框架上，将薄膜覆盖框架开口，并释放框架上的压力以使开口膨胀并放置薄膜 在紧张。

    Compositions for repelling fluid and uses thereof
    10.
    发明授权
    Compositions for repelling fluid and uses thereof 有权
    用于排斥液体的组合物及其用途

    公开(公告)号：US08263678B2

    公开(公告)日：2012-09-11

    申请号：US12641606

    申请日：2009-12-18

    申请人： Kofi A. Bissah ,  Ricardo De Oliveira ,  Saurabh Desai ,  Joseph J. LiBrizzi ,  Saroja Narasimhan ,  Thong Nguyen ,  Shoba Pillai

    发明人： Kofi A. Bissah ,  Ricardo De Oliveira ,  Saurabh Desai ,  Joseph J. LiBrizzi ,  Saroja Narasimhan ,  Thong Nguyen ,  Shoba Pillai

    IPC分类号： A61K8/37 ,  A61K31/215 ,  C08K5/10 ,  C08K5/101 ,  C08L83/06

    CPC分类号： A61L15/52 ,  A61K8/25 ,  A61K8/37 ,  A61K8/375 ,  A61K8/585 ,  A61K8/895 ,  A61L15/18 ,  A61L15/26 ,  A61Q15/00 ,  A61Q19/005 ,  C08L83/04

    摘要： Provided are compositions for repelling fluids comprising a volatile liquid carrier, a powder-feel agent and less than 5 weight % of an ester selected from the group consisting of formula I, formula II, formula III, and combinations of two or more thereof: wherein R1, R2, R3, R5, R6, R8 and R9 are independently linear or branched, substituted or unsubstituted, saturated or unsaturated, C3-C22 alkyl or alkenyl groups, R4 is a linear or branched, substituted or unsubstituted, saturated or unsaturated, C3-C22 alkylene or alkenylene moiety, and R7 is a linear or branched, substituted or unsubstituted, saturated or unsaturated C3-C22 moiety, the composition being substantially anhydrous. Also provided are methods of improving dryness and/or comfort associated with the intimate area, as well as, kits comprising the composition and an absorbent article.

    摘要翻译： 提供了用于排斥流体的组合物，其包含挥发性液体载体，粉末感受剂和小于5重量％的选自式I，式II，式III的酯，以及其两种或多种的组合：其中 R 1，R 2，R 3，R 5，R 6，R 8和R 9独立地是直链或支链的，取代或未取代的饱和或不饱和的C 3 -C 22烷基或链烯基，R 4是直链或支链的，取代或未取代的饱和或不饱和的， C3-C22亚烷基或亚烯基部分，并且R7是直链或支链的取代或未取代的饱和或不饱和的C 3 -C 22部分，该组合物基本上是无水的。 还提供了改善与紧密区域相关的干燥和/或舒适性的方法，以及包含该组合物和吸收制品的试剂盒。