公开(公告)号：US10039178B2

公开(公告)日：2018-07-31

申请号：US13183907

申请日：2011-07-15

申请人： Qinghua Liu ,  Zheng Duan ,  Zhang Yu

发明人： Qinghua Liu ,  Zheng Duan ,  Zhang Yu

IPC分类号： B23K10/00 ,  H05H1/34

CPC分类号： H05H1/34 ,  B23K10/006 ,  H05H2001/3494

摘要： A method of determining a failure event of consumable for a plasma torch is provided. The method includes monitoring at least one of an operating current or an operating voltage during a transfer arc mode of the plasma arc torch. The method also includes determining when at least one parameter associated with the operating current or the operating voltage exceeds a tolerance threshold for a time period indicative of the failure event. The method further includes shutting off at least one of the operating current or the operating voltage of the plasma arc torch when the at least one parameter exceeds the tolerance threshold for the time period.

公开(公告)号：US20120234803A1

公开(公告)日：2012-09-20

申请号：US13183907

申请日：2011-07-15

申请人： Qinghua Liu ,  Zheng Duan ,  Zhang Yu

发明人： Qinghua Liu ,  Zheng Duan ,  Zhang Yu

IPC分类号： B23K9/32

CPC分类号： H05H1/34 ,  B23K10/006 ,  H05H2001/3494

摘要： A method of determining a failure event of consumable for a plasma torch is provided. The method includes monitoring at least one of an operating current or an operating voltage during a transfer arc mode of the plasma arc torch. The method also includes determining when at least one parameter associated with the operating current or the operating voltage exceeds a tolerance threshold for a time period indicative of the failure event. The method further includes shutting off at least one of the operating current or the operating voltage of the plasma arc torch when the at least one parameter exceeds the tolerance threshold for the time period.

摘要翻译： 提供了一种确定等离子体焰炬消耗品故障事件的方法。 该方法包括在等离子弧焊炬的转移电弧模式期间监视工作电流或工作电压中的至少一个。 该方法还包括确定在与指示故障事件的时间段中何时与工作电流或工作电压相关联的至少一个参数超过公差阈值。 该方法还包括当至少一个参数超过该时间段的容限阈值时，关闭等离子弧焊炬的工作电流或工作电压中的至少一个。

公开(公告)号：US08454970B2

公开(公告)日：2013-06-04

申请号：US13144765

申请日：2009-01-19

申请人： Yanmei Li ,  Qinghua Liu

发明人： Yanmei Li ,  Qinghua Liu

IPC分类号： A61K36/00 ,  A61K36/02

CPC分类号： A61K31/336 ,  A61K31/355 ,  A61K31/737 ,  A61K36/02 ,  A61K2300/00

摘要： A composition comprises extract of fucoxanthin, wherein said composition further comprises tocotrienols and/or fucoidan. Said composition can be used to treat obesity. Said composition shows outstanding effect of weight loss. There is outstanding synergy effect among those constituents of the composition.

摘要翻译： 组合物包含岩藻黄素的提取物，其中所述组合物还包含生育三烯酚和/或岩藻依聚糖。 所述组合物可用于治疗肥胖症。 所述组合物显示出显着的减肥效果。 组合中的这些成分之间具有突出的协同作用。

公开(公告)号：US07144714B2

公开(公告)日：2006-12-05

申请号：US10670626

申请日：2003-09-25

申请人： Xiaodong Wang ,  Qinghua Liu

发明人： Xiaodong Wang ,  Qinghua Liu

IPC分类号： C12P19/34 ,  C12P21/06 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/111 ,  C07K14/43581 ,  C12N9/22 ,  C12N2310/14 ,  C12N2330/30 ,  C12N2799/026

摘要： Methods for making siRNA comprise recombinantly coexpressing a Dicer protein with an R2D2 protein to form a complex comprising the R2D2 protein and the Dicer protein, and contacting the complex with a double-stranded (ds) RNA under conditions wherein the complex cleaves the dsRNA into siRNA. The R2D2 protein may be a Drosophila R2D2 protein and the Dicer protein may be a Drosophila Dicer-2 protein. The Dicer protein and the R2D2 protein may be coexpressed in insect cells, such as S2, Sf9 or Hi5 cells, using a baculovirus expression system.

摘要翻译： 用于制备siRNA的方法包括用R2D2蛋白重组共表达Dicer蛋白以形成包含R2D2蛋白和Dicer蛋白的复合物，并且在复合物将dsRNA切割成siRNA的条件下使复合物与双链（ds）RNA接触 。 R2D2蛋白可以是果蝇R2D2蛋白，Dicer蛋白可以是果蝇Dicer-2蛋白。 Dicer蛋白和R2D2蛋白可以使用杆状病毒表达系统共同表达在昆虫细胞如S2，Sf9或Hi5细胞中。

公开(公告)号：US20050136447A1

公开(公告)日：2005-06-23

申请号：US10954721

申请日：2004-09-30

申请人： Stephen Elledge ,  Qinghua Liu

发明人： Stephen Elledge ,  Qinghua Liu

IPC分类号： C12N15/10 ,  C12N15/66 ,  C12N15/74 ,  C12N15/90 ,  C12Q1/68 ,  C12N15/85 ,  C12P21/04

CPC分类号： C12N15/905 ,  C12N15/10 ,  C12N15/66 ,  C12N15/74

摘要： The present invention provides compositions, including vectors, and methods for the rapid subcloning of nucleic acid sequences in vivo and in vitro. In particular, the invention provides vectors used to contain a gene of interest that comprise a sequence-specific recombinase target site. These vectors are used to rapidly transfer the gene or genes of interest into any vector that contains a sequence-specific recombinase target site located downstream of a regulatory element so that the gene of interest may be regulated.

摘要翻译： 本发明提供了在体内和体外快速亚克隆核酸序列的组合物，包括载体和方法。 特别地，本发明提供了用于含有包含序列特异性重组酶靶位点的目的基因的载体。 这些载体用于将感兴趣的基因或基因快速转移到任何载体中，所述载体含有位于调节元件下游的序列特异性重组酶靶位点，以使感兴趣的基因可以调节。

公开(公告)号：US20130030046A1

公开(公告)日：2013-01-31

申请号：US13576748

申请日：2010-11-11

申请人： Chunhua Li ,  Yanmei Li ,  Qinghua Liu

发明人： Chunhua Li ,  Yanmei Li ,  Qinghua Liu

IPC分类号： A61K31/352 ,  A61P19/02

CPC分类号： A61K9/0095 ,  A23L2/52 ,  A23L33/105 ,  A23V2002/00 ,  A61K36/48 ,  A23V2200/306

摘要： The application relates to a use of black soybean hull extracts in the manufacture of foods, health care products and medicaments. The black soybean hull extracts can be added to foods or drinks, and also can be made into all kinds of oral health care products and oral medicaments used by conventional methods of preparing traditional Chinese medicine preparations. The products can significantly improve symptoms of osteoarthritis, and also can stabilize or even reverse the degradation of cartilage of osteoarthritis.

摘要翻译： 本申请涉及黑豆皮提取物在制造食品，保健品和药物中的用途。 黑豆皮提取物可加入食品或饮料中，也可制成常规中药制剂使用的各种口腔保健品和口服药物。 该产品可显着改善骨关节炎的症状，也可稳定或甚至逆转骨关节炎软骨降解。

公开(公告)号：US20090215648A1

公开(公告)日：2009-08-27

申请号：US11784146

申请日：2007-04-04

申请人： Stephen J. Elledge ,  Qinghua Liu

发明人： Stephen J. Elledge ,  Qinghua Liu

IPC分类号： C40B40/08 ,  C12P19/34 ,  C12N15/00 ,  C12N15/87 ,  C40B50/06 ,  C12Q1/68 ,  C12N1/21

CPC分类号： C12N15/905 ,  C12N15/10 ,  C12N15/66 ,  C12N15/74

摘要： The present invention provides compositions, including vectors, and methods for the rapid subcloning of nucleic acid sequences in vivo and in vitro. In particular, the invention provides vectors used to contain a gene of interest that comprise a sequence-specific recombinase target site. These vectors are used to rapidly transfer the gene or genes of interest into any vector that contains a sequence-specific recombinase target site located downstream of a regulatory element so that the gene of interest may be regulated.

摘要翻译： 本发明提供了在体内和体外快速亚克隆核酸序列的组合物，包括载体和方法。 特别地，本发明提供了用于含有包含序列特异性重组酶靶位点的目的基因的载体。 这些载体用于将感兴趣的基因或基因快速转移到任何载体中，所述载体含有位于调节元件下游的序列特异性重组酶靶位点，以使感兴趣的基因可以调节。

公开(公告)号：US20050069990A1

公开(公告)日：2005-03-31

申请号：US10670626

申请日：2003-09-25

申请人： Xiaodong Wang ,  Qinghua Liu

发明人： Xiaodong Wang ,  Qinghua Liu

IPC分类号： C07K14/435 ,  C12N9/22 ,  C12N15/11 ,  C12P19/34

CPC分类号： C12N15/111 ,  C07K14/43581 ,  C12N9/22 ,  C12N2310/14 ,  C12N2330/30 ,  C12N2799/026

摘要： Methods for making siRNA comprise recombinantly coexpressing a Dicer protein with an R2D2 protein to form a complex comprising the R2D2 protein and the Dicer protein, and contacting the complex with a double-stranded (ds) RNA under conditions wherein the complex cleaves the dsRNA into siRNA. The R2D2 protein may be a Drosophila R2D2 protein and the Dicer protein may be a Drosophila Dicer-2 protein. The Dicer protein and the R2D2 protein may be coexpressed in insect cells, such as S2, Sf9 or Hi5 cells, using a baculovirus expression system.

摘要翻译： 用于制备siRNA的方法包括用R2D2蛋白重组共表达Dicer蛋白以形成包含R2D2蛋白和Dicer蛋白的复合物，并且在复合物将dsRNA切割成siRNA的条件下使复合物与双链（ds）RNA接触 。 R2D2蛋白可以是果蝇R2D2蛋白，Dicer蛋白可以是果蝇Dicer-2蛋白。 Dicer蛋白和R2D2蛋白可以使用杆状病毒表达系统共同表达在昆虫细胞如S2，Sf9或Hi5细胞中。

公开(公告)号：US5851808A

公开(公告)日：1998-12-22

申请号：US864224

申请日：1997-02-28

申请人： Stephen J. Elledge ,  Qinghua Liu

发明人： Stephen J. Elledge ,  Qinghua Liu

IPC分类号： C12N15/10 ,  C12N15/66 ,  C12N15/74 ,  C12N15/90 ,  C12N5/09 ,  C12N15/63 ,  C12N15/64

CPC分类号： C12N15/905 ,  C12N15/10 ,  C12N15/66 ,  C12N15/74

摘要： The present invention provides compositions, including vectors, and methods for the rapid subcloning of nucleic acid sequences in vivo and in vitro. In particular, the invention provides vectors used to contain a gene of interest that comprise a sequence-specific recombinase target site. These vectors are used to rapidly transfer the gene of interest into any expression vector that contains a sequence-specific recombinase target site located downstream of a promoter element so that the gene of interest may be expressed.

摘要翻译： 本发明提供了在体内和体外快速亚克隆核酸序列的组合物，包括载体和方法。 特别地，本发明提供了用于含有包含序列特异性重组酶靶位点的目的基因的载体。 这些载体用于将感兴趣的基因快速转移到包含位于启动子元件下游的序列特异性重组酶靶位点的任何表达载体中，以便可以表达感兴趣的基因。

公开(公告)号：US20120021953A1

公开(公告)日：2012-01-26

申请号：US12976637

申请日：2010-12-22

申请人： Stephen Elledge ,  Qinghua Liu

发明人： Stephen Elledge ,  Qinghua Liu

IPC分类号： C40B40/08 ,  C12N1/21 ,  C12N15/70 ,  C40B50/06 ,  C12N15/64 ,  C12N15/63

CPC分类号： C12N15/905 ,  C12N15/10 ,  C12N15/66 ,  C12N15/74

摘要： The present invention provides compositions, including vectors, and methods for the rapid subcloning of nucleic acid sequences in vivo and in vitro. In particular, the invention provides vectors used to contain a gene of interest that comprise a sequence-specific recombinase target site. These vectors are used to rapidly transfer the gene or genes of interest into any vector that contains a sequence-specific recombinase target site located downstream of a regulatory element so that the gene of interest may be regulated.

摘要翻译： 本发明提供了在体内和体外快速亚克隆核酸序列的组合物，包括载体和方法。 特别地，本发明提供了用于含有包含序列特异性重组酶靶位点的目的基因的载体。 这些载体用于将感兴趣的基因或基因快速转移到任何载体中，所述载体含有位于调节元件下游的序列特异性重组酶靶位点，以使感兴趣的基因可以调节。