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1.发明申请EXPRESSION OF FULL LENGTH IGG AND SECRETION INTO THE CULTURE MEDIUM OF PROKARYOTIC CELLS 审中-公开表达全长IGG和分泌到原核细胞培养物中公开(公告)号：US20100168392A1
公开(公告)日：2010-07-01
申请号：US12672859
申请日：2007-08-10
申请人： Ralf Ostendorp , Andreas Popp , Martina Fischer
发明人： Ralf Ostendorp , Andreas Popp , Martina Fischer
CPC分类号： C07K16/00 , C07K2317/10 , C07K2319/035
摘要： A method for the production of an immunoglobulin or a functional fragment thereof in a prokaryotic host cell comprises transforming the host cell with (a) a first nucleic acid molecule comprising a nucleic acid sequence encoding a VL and a CL region and (b) a second nucleic acid molecule comprising a nucleic acid sequence encoding a VH, a CH1, a CH2 and at least a portion of a CH3 region, The host cell is within culture medium. The host cell is cultured under conditions so as to allow the host cell (a) to express (1) the VL and a CL region and (2) the VH, the CH1, the CH2 and the portion of the CH3 region, and (b) to secrete (a)(1) and (a)(2) to the periplasm of the host cell and thereafter to the culture medium of the host cell. Characteristically, (a)(1) and (a)(2) interact to form the immunoglobulin or functional fragment thereof.
摘要翻译： 用于在原核宿主细胞中产生免疫球蛋白或其功能片段的方法包括用（a）包含编码VL和CL区的核酸序列的第一核酸分子转化宿主细胞，和（b）第二个 核酸分子，其包含编码VH，CH1，CH2和至少部分CH3区的核酸序列。宿主细胞在培养基内。 宿主细胞在条件下培养以允许宿主细胞（a）表达（1）VL和CL区和（2）VH，CH1，CH2和CH3区的部分，和（ b）将（a）（1）和（a）（2）分泌到宿主细胞的周质中，然后到宿主细胞的培养基。 特征地，（a）（1）和（a）（2）相互作用以形成免疫球蛋白或其功能片段。
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公开(公告)号：US20120077225A1
公开(公告)日：2012-03-29
申请号：US13064928
申请日：2011-04-27
申请人： Johann Brass , Joachim Klein , Ralf Ostendorp
发明人： Johann Brass , Joachim Klein , Ralf Ostendorp
CPC分类号： C07K14/70 , C07K16/00 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C12N15/52
摘要： New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.
摘要翻译： 可以在包含与包含与宿主异源的核酸序列的转录单位可操作地连接的蜜二糖操纵子的启动子区的宿主中表达的新载体。 核酸序列的表达由蜜二糖操纵子的启动子区域控制。 新载体可用于原核宿主中核酸序列的调节异源表达。 存在在宿主中可表达的分离和纯化的核酸序列，其包含可操作地连接到包含与宿主异源的核酸序列的转录单位的蜜二糖操纵子的启动子区。 核酸序列的表达由蜜二糖操纵子的启动子区域控制。 用载体或分离和纯化的核酸序列转化原核宿主。 存在使用载体在宿主中产生多肽的方法。
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公开(公告)号：US20080206817A1
公开(公告)日：2008-08-28
申请号：US11791989
申请日：2005-12-05
申请人： Johann Brass , Christoph Kiziak , Joachim Klein , Ralf Ostendorp
发明人： Johann Brass , Christoph Kiziak , Joachim Klein , Ralf Ostendorp
CPC分类号： C12N15/78 , C07K16/00 , C07K2317/14 , C07K2317/55 , C07K2317/622 , C12N15/63 , C12N15/70
摘要： Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: a) a nucleic acid sequence which is heterologouse to the host, and b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.
摘要翻译： 在宿主中可表达的是可操作地连接到转录单位的L-鼠李糖操纵子的rhaBAD启动子区的载体，所述转录单位是：a）与宿主异源的核酸序列，和b）可操作地连接到宿主的原核信号序列 核酸序列。 原核信号序列选自用于糖，氨基酸，维生素和离子的周质结合蛋白的信号肽。 核酸序列的表达由启动子区域控制。 该载体用于原核宿主中核酸序列的调节异源表达。 这是在宿主中可表达的分离和纯化的核酸序列是L-鼠李糖操纵子的启动子区。 存在使用载体在宿主中产生多肽的方法。
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公开(公告)号：US08735099B2
公开(公告)日：2014-05-27
申请号：US13064928
申请日：2011-04-27
申请人： Johann Brass , Joachim Klein , Ralf Ostendorp
发明人： Johann Brass , Joachim Klein , Ralf Ostendorp
CPC分类号： C07K14/70 , C07K16/00 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C12N15/52
摘要： New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.
摘要翻译： 可以在包含与包含与宿主异源的核酸序列的转录单位可操作地连接的蜜二糖操纵子的启动子区的宿主中表达的新载体。 核酸序列的表达由蜜二糖操纵子的启动子区域控制。 新载体可用于原核宿主中核酸序列的调节异源表达。 存在在宿主中可表达的分离和纯化的核酸序列，其包含可操作地连接到包含与宿主异源的核酸序列的转录单位的蜜二糖操纵子的启动子区。 核酸序列的表达由蜜二糖操纵子的启动子区域控制。 用载体或分离和纯化的核酸序列转化原核宿主。 存在使用载体在宿主中产生多肽的方法。
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公开(公告)号：US20080305526A1
公开(公告)日：2008-12-11
申请号：US11791988
申请日：2005-12-05
申请人： Johann Brass , Joachim Klein , Ralf Ostendorp
发明人： Johann Brass , Joachim Klein , Ralf Ostendorp
CPC分类号： C07K14/70 , C07K16/00 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C12N15/52
摘要： New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.
摘要翻译： 可以在包含与包含与宿主异源的核酸序列的转录单位可操作地连接的蜜二糖操纵子的启动子区的宿主中表达的新载体。 核酸序列的表达由蜜二糖操纵子的启动子区域控制。 新载体可用于原核宿主中核酸序列的调节异源表达。 存在在宿主中可表达的分离和纯化的核酸序列，其包含可操作地连接到包含与宿主异源的核酸序列的转录单位的蜜二糖操纵子的启动子区。 核酸序列的表达由蜜二糖操纵子的启动子区域控制。 用载体或分离和纯化的核酸序列转化原核宿主。 存在使用载体在宿主中产生多肽的方法。
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公开(公告)号：US09279128B2
公开(公告)日：2016-03-08
申请号：US13317293
申请日：2011-10-14
申请人： Johann Brass , Christoph Kiziak , Joachim Klein , Ralf Ostendorp
发明人： Johann Brass , Christoph Kiziak , Joachim Klein , Ralf Ostendorp
CPC分类号： C12N15/78 , C07K16/00 , C07K2317/14 , C07K2317/55 , C07K2317/622 , C12N15/63 , C12N15/70
摘要： Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: a) a nucleic acid sequence which is heterologous to the host, and b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.
摘要翻译： 在宿主中可表达的是可操作地连接到转录单位的L-鼠李糖操纵子的rhaBAD启动子区的载体，所述转录单位是：a）与宿主异源的核酸序列，和b）可操作地连接到宿主的原核信号序列 核酸序列。 原核信号序列选自用于糖，氨基酸，维生素和离子的周质结合蛋白的信号肽。 核酸序列的表达由启动子区域控制。 该载体用于原核宿主中核酸序列的调节异源表达。 这是在宿主中可表达的分离和纯化的核酸序列是L-鼠李糖操纵子的启动子区。 存在使用载体在宿主中产生多肽的方法。
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公开(公告)号：US20120135463A1
公开(公告)日：2012-05-31
申请号：US13317293
申请日：2011-10-14
申请人： Johann Brass , Christoph Kiziak , Joachim Klein , Ralf Ostendorp
发明人： Johann Brass , Christoph Kiziak , Joachim Klein , Ralf Ostendorp
CPC分类号： C12N15/78 , C07K16/00 , C07K2317/14 , C07K2317/55 , C07K2317/622 , C12N15/63 , C12N15/70
摘要： Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: a) a nucleic acid sequence which is heterologous to the host, and b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.
摘要翻译： 在宿主中可表达的是可操作地连接到转录单位的L-鼠李糖操纵子的rhaBAD启动子区的载体，所述转录单位是：a）与宿主异源的核酸序列，和b）可操作地连接到宿主的原核信号序列 核酸序列。 原核信号序列选自用于糖，氨基酸，维生素和离子的周质结合蛋白的信号肽。 核酸序列的表达由启动子区域控制。 该载体用于原核宿主中核酸序列的调节异源表达。 这是在宿主中可表达的分离和纯化的核酸序列是L-鼠李糖操纵子的启动子区。 存在使用载体在宿主中产生多肽的方法。
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