公开(公告)号：US20120077225A1

公开(公告)日：2012-03-29

申请号：US13064928

申请日：2011-04-27

申请人： Johann Brass ,  Joachim Klein ,  Ralf Ostendorp

发明人： Johann Brass ,  Joachim Klein ,  Ralf Ostendorp

IPC分类号： C12P21/00 ,  C12N1/00 ,  C12N15/63

CPC分类号： C07K14/70 ,  C07K16/00 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2319/00 ,  C12N15/52

摘要： New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.

摘要翻译： 可以在包含与包含与宿主异源的核酸序列的转录单位可操作地连接的蜜二糖操纵子的启动子区的宿主中表达的新载体。 核酸序列的表达由蜜二糖操纵子的启动子区域控制。 新载体可用于原核宿主中核酸序列的调节异源表达。 存在在宿主中可表达的分离和纯化的核酸序列，其包含可操作地连接到包含与宿主异源的核酸序列的转录单位的蜜二糖操纵子的启动子区。 核酸序列的表达由蜜二糖操纵子的启动子区域控制。 用载体或分离和纯化的核酸序列转化原核宿主。 存在使用载体在宿主中产生多肽的方法。

公开(公告)号：US20080206817A1

公开(公告)日：2008-08-28

申请号：US11791989

申请日：2005-12-05

申请人： Johann Brass ,  Christoph Kiziak ,  Joachim Klein ,  Ralf Ostendorp

发明人： Johann Brass ,  Christoph Kiziak ,  Joachim Klein ,  Ralf Ostendorp

IPC分类号： C12P21/00 ,  C12N15/63 ,  C12N15/00 ,  C07H21/00 ,  C12N1/00

CPC分类号： C12N15/78 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/55 ,  C07K2317/622 ,  C12N15/63 ,  C12N15/70

摘要： Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: a) a nucleic acid sequence which is heterologouse to the host, and b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.

摘要翻译： 在宿主中可表达的是可操作地连接到转录单位的L-鼠李糖操纵子的rhaBAD启动子区的载体，所述转录单位是：a）与宿主异源的核酸序列，和b）可操作地连接到宿主的原核信号序列 核酸序列。 原核信号序列选自用于糖，氨基酸，维生素和离子的周质结合蛋白的信号肽。 核酸序列的表达由启动子区域控制。 该载体用于原核宿主中核酸序列的调节异源表达。 这是在宿主中可表达的分离和纯化的核酸序列是L-鼠李糖操纵子的启动子区。 存在使用载体在宿主中产生多肽的方法。

公开(公告)号：US09279128B2

公开(公告)日：2016-03-08

申请号：US13317293

申请日：2011-10-14

申请人： Johann Brass ,  Christoph Kiziak ,  Joachim Klein ,  Ralf Ostendorp

发明人： Johann Brass ,  Christoph Kiziak ,  Joachim Klein ,  Ralf Ostendorp

IPC分类号： C12N15/10 ,  C12N15/00 ,  C12N15/70 ,  C07H21/02 ,  C12N15/63 ,  C12N15/78

CPC分类号： C12N15/78 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/55 ,  C07K2317/622 ,  C12N15/63 ,  C12N15/70

摘要： Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: a) a nucleic acid sequence which is heterologous to the host, and b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.

摘要翻译： 在宿主中可表达的是可操作地连接到转录单位的L-鼠李糖操纵子的rhaBAD启动子区的载体，所述转录单位是：a）与宿主异源的核酸序列，和b）可操作地连接到宿主的原核信号序列 核酸序列。 原核信号序列选自用于糖，氨基酸，维生素和离子的周质结合蛋白的信号肽。 核酸序列的表达由启动子区域控制。 该载体用于原核宿主中核酸序列的调节异源表达。 这是在宿主中可表达的分离和纯化的核酸序列是L-鼠李糖操纵子的启动子区。 存在使用载体在宿主中产生多肽的方法。

公开(公告)号：US20120135463A1

公开(公告)日：2012-05-31

申请号：US13317293

申请日：2011-10-14

申请人： Johann Brass ,  Christoph Kiziak ,  Joachim Klein ,  Ralf Ostendorp

发明人： Johann Brass ,  Christoph Kiziak ,  Joachim Klein ,  Ralf Ostendorp

IPC分类号： C12P21/06 ,  C12N1/21 ,  C07H21/04 ,  C12N15/74 ,  C12N15/70 ,  C12N15/75 ,  C12N15/78

CPC分类号： C12N15/78 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/55 ,  C07K2317/622 ,  C12N15/63 ,  C12N15/70

摘要： Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: a) a nucleic acid sequence which is heterologous to the host, and b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.

摘要翻译： 在宿主中可表达的是可操作地连接到转录单位的L-鼠李糖操纵子的rhaBAD启动子区的载体，所述转录单位是：a）与宿主异源的核酸序列，和b）可操作地连接到宿主的原核信号序列 核酸序列。 原核信号序列选自用于糖，氨基酸，维生素和离子的周质结合蛋白的信号肽。 核酸序列的表达由启动子区域控制。 该载体用于原核宿主中核酸序列的调节异源表达。 这是在宿主中可表达的分离和纯化的核酸序列是L-鼠李糖操纵子的启动子区。 存在使用载体在宿主中产生多肽的方法。

公开(公告)号：US08735099B2

公开(公告)日：2014-05-27

申请号：US13064928

申请日：2011-04-27

申请人： Johann Brass ,  Joachim Klein ,  Ralf Ostendorp

发明人： Johann Brass ,  Joachim Klein ,  Ralf Ostendorp

IPC分类号： C12P21/02 ,  C12N15/00 ,  C07H21/04

CPC分类号： C07K14/70 ,  C07K16/00 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2319/00 ,  C12N15/52

摘要： New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.

摘要翻译： 可以在包含与包含与宿主异源的核酸序列的转录单位可操作地连接的蜜二糖操纵子的启动子区的宿主中表达的新载体。 核酸序列的表达由蜜二糖操纵子的启动子区域控制。 新载体可用于原核宿主中核酸序列的调节异源表达。 存在在宿主中可表达的分离和纯化的核酸序列，其包含可操作地连接到包含与宿主异源的核酸序列的转录单位的蜜二糖操纵子的启动子区。 核酸序列的表达由蜜二糖操纵子的启动子区域控制。 用载体或分离和纯化的核酸序列转化原核宿主。 存在使用载体在宿主中产生多肽的方法。

公开(公告)号：US20080305526A1

公开(公告)日：2008-12-11

申请号：US11791988

申请日：2005-12-05

申请人： Johann Brass ,  Joachim Klein ,  Ralf Ostendorp

发明人： Johann Brass ,  Joachim Klein ,  Ralf Ostendorp

IPC分类号： C12P21/04 ,  C12N15/00 ,  C12N15/11 ,  C12N1/20

CPC分类号： C07K14/70 ,  C07K16/00 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2319/00 ,  C12N15/52

摘要： New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.

摘要翻译： 可以在包含与包含与宿主异源的核酸序列的转录单位可操作地连接的蜜二糖操纵子的启动子区的宿主中表达的新载体。 核酸序列的表达由蜜二糖操纵子的启动子区域控制。 新载体可用于原核宿主中核酸序列的调节异源表达。 存在在宿主中可表达的分离和纯化的核酸序列，其包含可操作地连接到包含与宿主异源的核酸序列的转录单位的蜜二糖操纵子的启动子区。 核酸序列的表达由蜜二糖操纵子的启动子区域控制。 用载体或分离和纯化的核酸序列转化原核宿主。 存在使用载体在宿主中产生多肽的方法。

公开(公告)号：US6119507A

公开(公告)日：2000-09-19

申请号：US231389

申请日：1999-01-13

申请人： Rudolf Flosbach ,  Joachim Klein ,  Markus Nothhelfer

发明人： Rudolf Flosbach ,  Joachim Klein ,  Markus Nothhelfer

IPC分类号： G01M3/20 ,  B01D15/08 ,  F15B1/02 ,  F25J3/00

CPC分类号： G01M3/205

摘要： A helium supply unit (1) supplies helium from a high pressure storage (21) to fill a test chamber (6) which encloses a sample holder (5) to be examined for leaks by measuring device (2) including a mass spectrometer (3) and a control unit (4). The test gas is recovered after the testing by a pressure difference between a low pressure storage (18) and the test chamber (6). The test chamber is evacuated prior to receipt of the test gas by a vacuum pump (27) and after testing by a vacuum pump (34) for more complete helium recovery. The recovered test gas is then transmitted back to the high pressure storage (21) through a compressor (19), which raises the pressure of the recovered gas to a predetermined pressure, from the low pressure storage (18). The control unit (4) also controls a pure test gas supply (37) and a release valve (44). The concentration of the recovered test gas is measured and, if the concentration is too low, the control unit (4) releases the recovered test gas through valve (44) and admits fresh test gas from the supply (37) into the helium supply unit (1) at a point between the low pressure storage (18) and the compressor (19). In this manner, expensive test gases, such as helium, are recovered and reused.

摘要翻译： 氦供应单元（1）从高压储存器（21）提供氦气以填充测试室（6），该测试室包围待检查的样品保持器（5），以通过包括质谱仪（3）的测量装置（2） ）和控制单元（4）。 在测试之后，通过低压储存器（18）和测试室（6）之间的压力差来回收测试气体。 在通过真空泵（27）接收测试气体之前，并且在通过真空泵（34）测试以更完整的氦气回收之后，将测试室抽真空。 回收的测试气体然后通过压缩机（19）传回到高压储存器（21），压缩机将回收气体的压力从低压储存器（18）提高到预定的压力。 控制单元（4）还控制纯试气供应（37）和释放阀（44）。 测量回收的测试气体的浓度，如果浓度太低，则控制单元（4）通过阀（44）释放回收的测试气体，并将新鲜的测试气体从供应源（37）导入氦气供应单元 （1）在低压储存器（18）和压缩机（19）之间的点处。 以这种方式，昂贵的测试气体，例如氦气被回收和重复使用。

公开(公告)号：US4639423A

公开(公告)日：1987-01-27

申请号：US667361

申请日：1984-11-01

申请人： Wolfgang Kahlert ,  Joachim Klein ,  Hans-Jurgen Steinert ,  Claus Vorlop

发明人： Wolfgang Kahlert ,  Joachim Klein ,  Hans-Jurgen Steinert ,  Claus Vorlop

IPC分类号： C12M3/02 ,  B01J2/06 ,  B01J2/10 ,  C12M1/40 ,  C12N11/00 ,  C12M1/00

CPC分类号： C12M25/16 ,  C12M23/34 ,  C12M23/38 ,  C12M27/02 ,  C12M29/04 ,  C12M37/00 ,  C12M41/12

摘要： An apparatus for the production of biocatalyst beads comprises an immobilization vessel (10) which is provided at its lower end with discharge pipes (23) which extend through a pressure chamber (25) in which a gas pressure prevails. The pressure chamber (25) is terminated by a plate (24) exhibiting holes which coaxially surround the discharge pipes (23). At the lower end of the immobilization vessel (10) is provided a connecting nozzle (30) which is connected in germproof sealing manner to the wall of a collecting vessel (11) which contains a solution of a cross-linking agent or a reprecipitation bath. The collecting vessel (11) may be constructed as a fermenter and provided with an agitator. Both of the vessels may be jointly sterilized.

摘要翻译： 用于生产生物催化剂珠的装置包括固定容器（10），其在其下端设置有排出管（23），排出管（23）延伸通过其中气体压力占优势的压力室（25）。 压力室（25）由同轴地围绕放电管（23）的孔的板（24）端接。 在固定容器（10）的下端设置连接喷嘴（30），该连接喷嘴（30）以防菌密封方式连接到收集容器（11）的壁上，该收集容器（11）包含交联剂或再沉淀浴 。 收集容器（11）可以构造为发酵罐并设置有搅拌器。 这两种容器都可以联合灭菌。

公开(公告)号：US08812492B2

公开(公告)日：2014-08-19

申请号：US13330860

申请日：2011-12-20

申请人： Joachim Klein

发明人： Joachim Klein

IPC分类号： G06F17/30

CPC分类号： G06F17/3048

摘要： A system and method is provided for automatic and dynamic adaptation of cache groups in a database system having one or more processors. The method includes analyzing a database query and determining if a set of predicates in a predicate pattern are suitable for inclusion in one or more cache groups, with the one or more cache groups having one or more cache tables; mapping value-based predicates in the predicate pattern to a filling column in the one or more cache tables; and mapping equi-join predicates in the predicate pattern to a referential cache constraint in the one or more cache tables. New cache groups can be created for predicate patterns occurring more frequently and existing cache groups can be deleted if the frequency of the predicate pattern falls below a predetermined threshold value.

摘要翻译： 提供了一种用于在具有一个或多个处理器的数据库系统中对高速缓存组进行自动和动态适配的系统和方法。 该方法包括分析数据库查询并确定谓词模式中的一组谓词是否适于包含在一个或多个高速缓存组中，一个或多个高速缓存组具有一个或多个高速缓存表; 将谓词模式中的基于值的谓词映射到一个或多个缓存表中的填充列; 并将谓词模式中的等价连接谓词映射到一个或多个缓存表中的引用高速缓存约束。 可以为更频繁发生的谓词模式创建新的高速缓存组，如果谓词模式的频率低于预定阈值，则可以删除现有高速缓存组。

公开(公告)号：US6152684A

公开(公告)日：2000-11-28

申请号：US63599

申请日：1998-04-21

申请人： Jean-Marc Ferme ,  Joachim Klein ,  Eberhard Kopf

发明人： Jean-Marc Ferme ,  Joachim Klein ,  Eberhard Kopf

IPC分类号： F03B11/04 ,  F03B15/06 ,  F03B15/16 ,  F01D17/00 ,  F04D31/00

CPC分类号： F03B15/06 ,  F03B11/04 ,  F03B15/16 ,  Y02E10/226

摘要： A process for operating a hydraulic machine, especially a hydro-turbine, includes the step of detecting an actual machine state during operation of the machine by ascertaining operating data. The ascertained operating data includes flow through the turbine (if present), effective and reactive power fed into the electric mains, guide vane opening, pressure before the machine and at the suction pipe exit, headwater level, and tailwater level. The method further includes the step of processing at least one state characteristic values of a first cavitation characteristic value (indicative of cavitation intensity), a second cavitation characteristic value (indicative of an abrupt rise in cavitation manifestations), vibration characteristic values, suction pipe pressure, sand concentration, air pressure, water temperature, and generator temperature (for example winding temperature). The method also includes the step of altering an operating point of the machine based on the at least one processed characteristic value to reduce cavitation and/or quiet running.

摘要翻译： 用于操作液压机，特别是水轮机的过程包括通过确定操作数据来检测机器操作期间的实际机器状态的步骤。 确定的操作数据包括通过涡轮机（如果有的话）的流量，进入电源的有效和无功功率，导向叶片开度，机器之前和吸入管出口处的压力，上游水位和尾水水位。 该方法还包括处理第一气穴特征值（指示空化强度），第二空化特征值（指示空化表现的突然升高），振动特性值，吸入管压力的至少一个状态特征值的步骤 ，砂浓度，空气压力，水温和发生器温度（例如绕组温度）。 该方法还包括基于至少一个经处理的特征值来改变机器的操作点以减少气穴和/或安静行驶的步骤。