中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

11 records (took 0.017 seconds)

    DETECTION OF HYDROXYMETHYLCYTOSINE BASES
    1.
    发明申请
    DETECTION OF HYDROXYMETHYLCYTOSINE BASES 有权

    公开(公告)号：US20210371906A1

    公开(公告)日：2021-12-02

    申请号：US17400168

    申请日：2021-08-12

    Applicant: Ramot at Tel-Aviv University Ltd.

    Inventor： Yuval EBENSTEIN ,  Micha FRIDMAN

    IPC: C12Q1/6806 ,  C12P19/30

    Abstract: Methodologies for labeling the epigenetic modification 5-hydroxymethyl-cytosine (5hmC) along a DNA molecule, and for determining a presence or a level of this epigenetic modification based on a ratio of fluorescence intensity of a labeled DNA sample to absorption intensity of the DNA sample at 260 nm are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.

    METHODS OF DETECTING METHYLATED CpG
    5.
    发明公开
    METHODS OF DETECTING METHYLATED CpG 审中-公开

    公开(公告)号：US20230220459A1

    公开(公告)日：2023-07-13

    申请号：US18009013

    申请日：2021-06-10

    Applicant: Ramot at Tel-Aviv University Ltd.

    Inventor： Yuval EBENSTEIN ,  Shahar ZIRKIN ,  Yael MICHAELI-HOCH ,  Noa GILAT ,  Orit FELDSTEIN ,  Adaya BEN-MOSHE

    IPC: C12Q1/6858

    CPC classification number: C12Q1/6858 ,  C12Q1/6806

    Abstract: Methods of detecting methylated CpG are provided. Accordingly, there is provided a method of determining CpG methylation status in a DNA sample, the method comprising: (a) subjecting the DNA sample to bisulfite conversion; (b) amplifying said DNA sample following said (a) to obtain an amplified DNA sample; (c) labeling CpG sites in said amplified DNA sample with a label to obtain a labeled DNA sample; (d) contacting said labeled DNA sample on an array comprising a plurality of probes for said DNA under conditions which allow specific hybridization between said plurality of probes and said DNA; and (e)detecting said hybridization, wherein an amount of said label is indicative of the CpG methylation status in said DNA sample.

    METHODS AND ARRAYS FOR IDENTIFYING THE CELL OR TISSUE ORIGIN OF DNA
    7.
    发明申请
    METHODS AND ARRAYS FOR IDENTIFYING THE CELL OR TISSUE ORIGIN OF DNA 有权

    公开(公告)号：US20230047930A1

    公开(公告)日：2023-02-16

    申请号：US17788003

    申请日：2020-12-22

    Applicant: Ramot at Tel-Aviv University Ltd.

    Inventor： Yuval EBENSTEIN ,  Shahar ZIRKIN ,  Noa GILAT ,  Yael MICHAELI HOCH

    IPC: C12Q1/6881 ,  C12Q1/6886

    Abstract: Methods and arrays for identifying the cell or tissue origin of DNA are provided. Accordingly there is provided a method of identifying DNA having a methylation pattern distinctive of a cell or tissue type or state comprising: labeling an epigenetic modification of interest in a DNA sample with a label; contacting said sample on an array comprising a plurality of probes for said DNA under conditions which allow specific hybridization between said plurality of probes and said DNA; and detecting said hybridization, wherein an amount of said label is indicative of the cell or tissue type or state, wherein the method is effected in the absence of amplification of said DNA.

    MULTI-SAMPLE ARRAY CHIP FOR DNA MODIFICATION QUANTIFICATION
    10.
    发明申请
    MULTI-SAMPLE ARRAY CHIP FOR DNA MODIFICATION QUANTIFICATION 有权

    公开(公告)号：US20210254167A1

    公开(公告)日：2021-08-19

    申请号：US16972691

    申请日：2019-06-06

    Applicant: Ramot at Tel-Aviv University Ltd.

    Inventor： Yuval EBENSTEIN ,  Shahar ZIRKIN ,  Sapir MARGALIT ,  Yael MICHAELI HOCH

    IPC: C12Q1/6886 ,  C12Q1/6834 ,  G01N33/68 ,  G01N33/58

    Abstract: A method of detecting an epigenetic DNA modification is described herein, the method comprising: attaching to DNA a labeling agent selective for the DNA modification; contacting an aqueous solution comprising the DNA with at least one addressable region on a surface of a substrate, wherein the addressable region comprises a positively charged substance capable of attaching the DNA to the surface, and the addressable region is surrounded by a hydrophobic region; and determining an amount of labeling agent in the addressable region. Further described herein is a kit comprising a substrate comprising discrete hydrophilic regions separated by a hydrophobic region, and a labeling agent selective for an epigenetic DNA modification; as well as an article of manufacture comprising a substrate comprising a discrete regions separated by a hydrophobic region, a positively charged substance and DNA attached thereto, the DNA comprising such a labeling agent.

Patent Agency Ranking