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10 条记录 (耗时 0.048 秒)

    Modular assembly of antibody genes, antibodies prepared thereby and use
    2.
    发明授权
    Modular assembly of antibody genes, antibodies prepared thereby and use 失效
    抗体基因的模块化装配，由此制备的抗体和使用

    公开(公告)号：US06204023B1

    公开(公告)日：2001-03-20

    申请号：US08472691

    申请日：1995-06-06

    申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

    发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

    IPC分类号： A61K39395

    CPC分类号： C12N15/70 ,  A61K38/00 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30 ,  C12N9/88 ,  C12N15/81

    摘要： The invention relates to cDNA genetic sequences, vehicles containing same as well as hosts tansformed therewith, for the production of chimeric immunoglobulin molecules, functional fragments thereof and immunoglobulin derivatives exhibiting novel functional properties comprising human constant region modules and non-human variable region modules, or for class switching antibody molecules and/or chains. The invention also relates to DNA coding for pectate lyase signal peptide has been cloned on a plasmid to create a secretion vector which is capable of producing a chosen protein which is transported across the bacterial membrane. The secretion vector has been used to secrete extracellular thaumatin and extracellular chimeric antibody fragments. The proteins produced by this vector have biological activity. The thaumatin is properly folded and the antibody fragments are capable of binding antigens on target cancer cells. The invention also relates to the secretion of chimeric antibodies and fragments thereof from yeast in functional form.

    摘要翻译： 本发明涉及cDNA遗传序列，含有其的载体以及与其一起改造的宿主，用于产生嵌合免疫球蛋白分子，其功能片段和表现出包括人恒定区模块和非人可变区模块的新功能特性的免疫球蛋白衍生物，或 用于类交换抗体分子和/或链。本发明还涉及将编码果胶酶裂解酶信号肽的DNA克隆在质粒上以产生能够产生选择的蛋白质的分泌载体，所述蛋白质通过细菌膜传输。 已经使用分泌载体来分泌细胞外的奇异果甜蛋白和细胞外嵌合抗体片段。 由该载体产生的蛋白质具有生物活性。 正确地折叠奇异果甜蛋白，抗体片段能够结合靶癌细胞上的抗原。 本发明还涉及从功能形式的酵母中分泌嵌合抗体及其片段。

    Horizontal slab gel electrophoresis
    9.
    发明授权
    Horizontal slab gel electrophoresis 失效
    水平平板凝胶电泳

    公开(公告)号：US4151065A

    公开(公告)日：1979-04-24

    申请号：US873448

    申请日：1978-01-30

    申请人： Donald A. Kaplan ,  Gary L. Wilcox

    发明人： Donald A. Kaplan ,  Gary L. Wilcox

    IPC分类号： G01N27/447 ,  G01N27/26 ,  G01N27/28

    CPC分类号： G01N27/44756

    摘要： An improved horizontal slab gel electrophoresis apparatus having compartments for vertical wicks at each end of a removable horizontal trough. Adjacent to the vertical wicks are vertical buffer compartments with built in electrodes. A partition separates the vertical wirks from the buffer compartment and includes a slot with a silicone plug which can be removed after curing or hardening of the vertical wicks to permit communication between the buffer and the gel slab. Flow adapters and an interconnecting tube permit constant circulation of the buffer to maintain equal volume and pH. The horizontal trough has slots for insertion of combs to form up to seventy-five sample wells and additional slots at each end for sealing the end of the horizontal slab when the gel slab is removed. The apparatus includes coils in a horizontal bed or plate beneath the trough for cooling the gel slab during a run and levelling screws for levelling the horizontal trough before pouring the gel slab.

    摘要翻译： 一种改进的水平平板凝胶电泳装置，其具有在可移除的水平槽的每一端处用于垂直芯的隔室。 与垂直灯芯相邻的是内置电极的垂直缓冲隔间。 隔板将垂直织带与缓冲隔间分开，并且包括具有硅胶塞的槽，其可以在固化或硬化垂直芯之后被去除，以允许缓冲器和凝胶板之间的连通。 流动适配器和互连管允许缓冲液的恒定循环以保持相等的体积和pH。 水平槽具有用于插入梳子以形成多达七十五个样品孔的槽，并且在每个端部具有另外的槽，用于在去除凝胶板时密封水平板的端部。 该设备包括在槽下面的水平床或板中的线圈，用于在运行期间冷却凝胶板，并且在调整螺钉之前，在浇注凝胶板之前调平水平槽。

    Horizontal slab gel electrophoresis
    10.
    发明授权
    Horizontal slab gel electrophoresis 失效
    水平平板凝胶电泳

    公开(公告)号：US4234400A

    公开(公告)日：1980-11-18

    申请号：US6826

    申请日：1979-01-26

    申请人： Donald A. Kaplan ,  Gary L. Wilcox

    发明人： Donald A. Kaplan ,  Gary L. Wilcox

    IPC分类号： G01N27/447 ,  G01N27/26 ,  G01N27/28

    CPC分类号： G01N27/44782 ,  G01N27/44756

    摘要： An improved horizontal slab gel electrophoresis apparatus having compartments for vertical wicks at each end of a removable horizontal trough. Adjacent to the vertical wicks are vertical buffer compartments with built in electrodes. A partition separates the vertical wicks from the buffer compartment and includes a slot with a silicone plug which can be removed after curing or hardening of the vertical wicks to permit communication between the buffer and the gel slab. Flow adapters and an interconnecting tube permit constant circulation of the buffer to maintain equal volume and pH. The horizontal trough has slots for insertion of combs to form up to seventy-five sample wells and additional slots at each end for sealing the end of the horizontal slab when the gel slab is removed. The apparatus includes coils in a horizontal bed or plate beneath the trough for cooling the gel slab during a run and levelling screws for levelling the horizontal trough before pouring the gel slab.

    摘要翻译： 一种改进的水平平板凝胶电泳装置，其具有在可移除的水平槽的每一端处用于垂直芯的隔室。 与垂直灯芯相邻的是内置电极的垂直缓冲隔间。 隔板将垂直芯与缓冲隔间分开，并且包括具有硅胶塞的槽，其可以在固化或硬化垂直芯之后被去除以允许缓冲器和凝胶板之间的连通。 流动适配器和互连管允许缓冲液的恒定循环以保持相等的体积和pH。 水平槽具有用于插入梳子以形成多达七十五个样品孔的槽，并且在每个端部具有另外的槽，用于在去除凝胶板时密封水平板的端部。 该设备包括在槽下面的水平床或板中的线圈，用于在运行期间冷却凝胶板，并且在调整螺钉之前，在浇注凝胶板之前调平水平槽。