Method for detecting mutagenic substances
    8.
    发明申请
    Method for detecting mutagenic substances 审中-公开
    诱变物质检测方法

    公开(公告)号:US20050070006A1

    公开(公告)日:2005-03-31

    申请号:US10482790

    申请日:2002-06-25

    摘要: The invention relates to a method and means for the rapid identification of mutations. The test strains used in the invention contain a reporter system and a selection marker. An antibiotic resistance protein which after mutation allows the selective growth of the revertant and the targeted induction of the inducible reporter system of the developing revertants acts as the selection marker. Proteins that are capable of directly or indirectly triggering a measuring signal are used as the reporters. The mutations are identified by means of the reporter signal of the developing revertants, in such a way that the evaluation can take place after only a few hours.

    摘要翻译: 本发明涉及快速鉴定突变的方法和手段。 本发明中使用的测试菌株含有报道系统和选择标记。 突变后的抗生素抗性蛋白质允许回复体的选择性生长,并且靶向诱导发育中的反转体的诱导型报告系统作为选择标记物。 能够直接或间接触发测量信号的蛋白质被用作记者。 通过发展中的回复体的报道信号鉴定突变,使得评价仅在几小时后才能发生。