公开(公告)号：US20180148677A1

公开(公告)日：2018-05-31

申请号：US15659562

申请日：2017-07-25

Applicant: Repligen Corporation

Inventor： Shashi Kiran Kudugunti ,  WengLong Roy Lin ,  John Bonham-Carter ,  James R. Rusche

IPC: C12M1/26 ,  C12M1/00 ,  C12N5/00

CPC classification number: C12M33/14 ,  B01D61/145 ,  B01D63/02 ,  B01D65/08 ,  B01D2315/10 ,  B01D2321/2083 ,  C07K1/34 ,  C07K16/00 ,  C07K2317/14 ,  C12M29/04 ,  C12M29/10 ,  C12M29/16 ,  C12M47/10 ,  C12N5/0018 ,  C12N2510/02 ,  C12N2511/00 ,  F04B43/02 ,  F04B43/06 ,  F04B53/16

Abstract: Methods and systems of harvesting a cell product from a cell culture by culturing cells in a fluid medium until the cells have produced a cell product at a harvest concentration are disclosed. The cells are cultured in a cell culture system including a bioreactor connected to an ATF device. The methods include draining fluid medium from the bioreactor through the outlet and the ATF device until the bioreactor volume reaches a predetermined volume, and the ATF column yields at an ATF outlet a liquid containing cell product and passes fluid medium with a concentration of cell product that is lower than the harvest concentration back into the bioreactor, extracting the liquid containing cell product from the ATF outlet, refilling the bioreactor with sterile phosphate buffered saline or fluid medium without any cell product, and repeating steps until a desired amount of cell product has been removed.

公开(公告)号：US20220325708A1

公开(公告)日：2022-10-13

申请号：US17731295

申请日：2022-04-28

Applicant: Repligen Corporation

Inventor： Shashi Kiran Kudugunti ,  WengLong Roy Lin ,  John Bonham-Carter ,  James R. Rusche

IPC: F04B43/06 ,  C07K1/34 ,  B01D63/02 ,  C12M1/00 ,  F04B53/16 ,  B01D61/14 ,  B01D65/08 ,  C12M1/26 ,  C12N5/00

Abstract: Methods and systems of harvesting a cell product from a cell culture by culturing cells in a fluid medium until the cells have produced a cell product at a harvest concentration are disclosed. The cells are cultured in a cell culture system including a bioreactor connected to an ATF device. The methods include draining fluid medium from the bioreactor through the outlet and the ATF device until the bioreactor volume reaches a predetermined volume, and the ATF column yields at an ATF outlet a liquid containing cell product and passes fluid medium with a concentration of cell product that is lower than the harvest concentration back into the bioreactor, extracting the liquid containing cell product from the ATF outlet, refilling the bioreactor with sterile phosphate buffered saline or fluid medium without any cell product, and repeating steps until a desired amount of cell product has been removed.

公开(公告)号：US11339775B2

公开(公告)日：2022-05-24

申请号：US15659562

申请日：2017-07-25

Applicant: Repligen Corporation

Inventor： Shashi Kiran Kudugunti ,  WengLong Roy Lin ,  John Bonham-Carter ,  James R. Rusche

IPC: C12N5/00 ,  F04B43/06 ,  C07K1/34 ,  B01D63/02 ,  C12M1/00 ,  F04B53/16 ,  B01D61/14 ,  B01D65/08 ,  C12M1/26 ,  C07K16/00

Abstract: Methods and systems of harvesting a cell product from a cell culture by culturing cells in a fluid medium until the cells have produced a cell product at a harvest concentration are disclosed. The cells are cultured in a cell culture system including a bioreactor connected to an ATF device. The methods include draining fluid medium from the bioreactor through the outlet and the ATF device until the bioreactor volume reaches a predetermined volume, and the ATF column yields at an ATF outlet a liquid containing cell product and passes fluid medium with a concentration of cell product that is lower than the harvest concentration back into the bioreactor, extracting the liquid containing cell product from the ATF outlet, refilling the bioreactor with sterile phosphate buffered saline or fluid medium without any cell product, and repeating steps until a desired amount of cell product has been removed.

公开(公告)号：US20130317003A1

公开(公告)日：2013-11-28

申请号：US13843261

申请日：2013-03-15

Applicant: Repligen Corporation

Inventor： Vincent Jacques ,  James R. Rusche ,  Norton P. Peet ,  Jasbir Singh

IPC: C07D513/04 ,  C07D401/06 ,  C07D403/06 ,  C07D471/04 ,  C07D231/12

CPC classification number: C07D513/04 ,  C07D205/06 ,  C07D211/70 ,  C07D211/78 ,  C07D231/12 ,  C07D261/08 ,  C07D263/32 ,  C07D277/30 ,  C07D277/34 ,  C07D305/10 ,  C07D401/06 ,  C07D403/06 ,  C07D403/10 ,  C07D451/02 ,  C07D471/04 ,  C07D471/08

Abstract: This invention relates to generally inhibiting histone deacetylase (“HDAC”) enzymes (e.g., HDAC1, HDAC2, and HDAC3).

Abstract translation: 本发明涉及通常抑制组蛋白脱乙酰酶（“HDAC”）酶（例如HDAC1，HDAC2和HDAC3）。