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    Paratransgenesis to Control Termites or Other Social Insects
    6.
    发明申请
    Paratransgenesis to Control Termites or Other Social Insects 审中-公开
    控制白蚁或其他社会昆虫的副作用

    公开(公告)号：US20090047235A1

    公开(公告)日：2009-02-19

    申请号：US12120717

    申请日：2008-05-15

    申请人： Claudia R. Husseneder ,  James A. Ottea ,  Lane D. Foil ,  Frederick M. Enright ,  Richard K. Cooper

    发明人： Claudia R. Husseneder ,  James A. Ottea ,  Lane D. Foil ,  Frederick M. Enright ,  Richard K. Cooper

    IPC分类号： A01N25/00 ,  A01N63/04

    CPC分类号： A01N63/04 ,  A01N25/006 ,  A01N2300/00

    摘要： A paratransgenesis system is disclosed to kill targeted social insects such as termites and cockroaches, for example the Formosan subterranean termite. A genetically modified yeast can be effectively used to express and deliver lytic peptides directly within the termite gut. Some highly lytic peptides directly damage the insect gut itself, leading to the death of the insect within about three days. Other lytic peptides kill all (or at least most) species of protozoa in the termite gut. The protozoa provide wood-digesting enzymes (cellulases) to the termite. Without these protozoa (and their cellulases) the insect dies within about six weeks. The system is completely free from conventional neurotoxins and other organic pesticides.

    摘要翻译： 披露了一种副作用系统来杀死靶向的社会昆虫，如白蚁和蟑螂，例如台山地下白蚁。 遗传修饰的酵母可以有效地用于在白蚁肠内直接表达和递送裂解肽。 一些高度溶解的肽直接损害昆虫肠道本身，导致昆虫在三天内死亡。 其他裂解肽杀死白蚁中的所有（或至少大多数）原生动物。 原生动物向白蚁提供木材消化酶（纤维素酶）。 没有这些原生动物（和它们的纤维素酶），大约六周内昆虫死亡。 该系统完全不含常规神经毒素和其他有机农药。

    Transgenic fish capable of expressing exogenous lytic peptides
    8.
    发明授权
    Transgenic fish capable of expressing exogenous lytic peptides 失效
    能够表达外源性裂解肽的转基因鱼

    公开(公告)号：US5998698A

    公开(公告)日：1999-12-07

    申请号：US491609

    申请日：1995-06-07

    申请人： Richard K. Cooper ,  Frederick M. Enright

    发明人： Richard K. Cooper ,  Frederick M. Enright

    IPC分类号： A01K67/027 ,  C07K14/435 ,  C12N15/85 ,  C12N15/00

    CPC分类号： C12N15/8509 ,  A01K67/0275 ,  C07K14/43563 ,  A01K2217/05 ,  A01K2227/40 ,  A01K2267/02

    摘要： Novel means have been discovered for increasing the resistance of an animal host (including humans) to diseases caused by intracellular bacteria, protozoa, and viruses. The infection treated may, for example, be equine infectious anemia, or infection by the human immunodeficiency virus. Novel means have also been found for treating tumors Augmentation of the host's defenses against infectious diseases or tumors is achieved by "arming" the host's cells with an exogenous gene encoding a natural or synthetic lytic peptide. For example, the transfection of hematopoietic stem cells and embryonic cells will produce animals with enhanced disease resistance; and transfection of TIL (tumor infiltrating lymphocytes) cells or other cells can be used in the treatment of tumors. Genes coding for a cecropin or other native or synthetic lytic peptide can be transferred and stably expressed in mammalian, bony fish, other vertebrate, and other animal cells. The transformed cells have the ability to produce and secrete a broad spectrum chemotherapeutic agent that has a systemic effect on certain pathogens, particularly pathogens that might otherwise evade or overcome host defenses.

    摘要翻译： 已经发现了增加动物宿主（包括人类）对由细胞内细菌，原生动物和病毒引起的疾病的抵抗力的新方法。 所治疗的感染可能是例如马传染性贫血或人类免疫缺陷病毒的感染。 还发现了治疗肿瘤的新手段。通过用编码天然或合成裂解肽的外源基因“宿主”宿主的细胞来实现宿主对传染病或肿瘤防御的增强。 例如，造血干细胞和胚胎细胞的转染将产生具有增强的抗病性的动物; 并且转染TIL（肿瘤浸润淋巴细胞）细胞或其他细胞可用于治疗肿瘤。 编码天蚕素或其他天然或合成裂解肽的基因可以在哺乳动物，骨鱼，其他脊椎动物和其他动物细胞中转移并稳定表达。 转化的细胞具有产生和分泌广谱化学治疗剂的能力，该化学治疗剂对某些病原体尤其是可能逃避或克服宿主防御的病原体具有全身作用。

    Transformed mammalian cells capable of expressing cecropin b
    9.
    发明授权
    Transformed mammalian cells capable of expressing cecropin b 失效
    能够表达天蚕素的转化的哺乳动物细胞b

    公开(公告)号：US5556782A

    公开(公告)日：1996-09-17

    申请号：US450252

    申请日：1995-05-25

    申请人： Richard K. Cooper ,  Frederick M. Enright

    发明人： Richard K. Cooper ,  Frederick M. Enright

    IPC分类号： A61K38/00 ,  C07K14/435 ,  C12N15/82 ,  C12N5/10 ,  C12N15/85

    CPC分类号： C12N15/8281 ,  C07K14/43563 ,  A61K38/00

    摘要： Novel means have been discovered for increasing the resistance of a mammalian host (including humans) to diseases caused by intracellular bacteria, protozoa, and viruses. Novel means have also been found for treating tumors. Augmentation of the host's defenses against infectious diseases or tumors is achieved by "arming" the host's cells with a gene encoding the lytic peptide cecropin B. The host's own leukocytes, other cells involved in resistance to infection, or other cells are transformed, and expression of the gene is induced when needed to combat pathogens. Transfection of hematopoietic stem cells with the cecropin B gene will enhance disease resistance in mammals; and transfection of TIL (tumor infiltrating lymphocytes) cells or other cells can be used in the treatment of tumors. The transformed cells have the ability to produce and secrete a broad spectrum chemotherapeutic agent which has a systemic effect on certain pathogens, particularly pathogens that might otherwise evade or overcome host defenses. The peptide's expression is preferably induced only in areas of infection, where it will most effectively augment the host's defense systems. Expression of the exogenous gene does not damage a healthy recipient cell.

    摘要翻译： 为了增加哺乳动物宿主（包括人类）对由细胞内细菌，原生动物和病毒引起的疾病的抵抗力，已经发现了新的手段。 还发现了新的治疗肿瘤的方法。 通过用编码溶菌肽杀菌素B的基因“宿主”宿主的细胞来实现宿主防治传染病或肿瘤的增强。宿主自身的白细胞，涉及感染的其他细胞或其他细胞被转化，表达 的基因是在需要对抗病原体时被诱导的。 造血干细胞与天蚕素B基因的转染将增强哺乳动物的抗病能力; 并且转染TIL（肿瘤浸润淋巴细胞）细胞或其他细胞可用于治疗肿瘤。 转化的细胞具有产生和分泌广谱化学治疗剂的能力，其对某些病原体特别是可能逃避或克服宿主防御的病原体具有全身作用。 肽的表达优选仅在感染区域诱导，其中最有效地增加宿主的防御系统。 外源基因的表达不损伤健康的受体细胞。

    Ligand/lytic peptide methods of use
    10.
    发明授权
    Ligand/lytic peptide methods of use 有权
    配体/溶解肽的使用方法

    公开(公告)号：US08258100B2

    公开(公告)日：2012-09-04

    申请号：US12488353

    申请日：2009-06-19

    申请人： Frederick M. Enright ,  Jesse M. Jaynes ,  William Hansel ,  Kenneth L. Koonce ,  Samuel M. McCann ,  Wen H. Yu ,  Patricia A. Melrose ,  Lane D. Foil ,  Philip H. Elzer

    发明人： Frederick M. Enright ,  Jesse M. Jaynes ,  William Hansel ,  Kenneth L. Koonce ,  Samuel M. McCann ,  Wen H. Yu ,  Patricia A. Melrose ,  Lane D. Foil ,  Philip H. Elzer

    IPC分类号： A61K38/00 ,  A61K38/25

    CPC分类号： C12N15/8257 ,  A61K38/00 ,  C07K7/23 ,  C07K14/43563 ,  C07K14/575 ,  C07K14/59 ,  C07K19/00 ,  C07K2319/00

    摘要： Amphipathic lytic peptides are ideally suited to use in a ligand/cytotoxin combination to specifically inhibit cells that are driven by or are dependent upon a specific ligand interaction; for example, to induce sterility or long-term contraception, or to attack tumor cells, or to selectively lyse virally-infected cells, or to attack lymphocytes responsible for autoimmune diseases. The peptides act directly on cell membranes, and need not be internalized. Administering a combination of gonadotropin-releasing hormone (GnRH) (or a GnRH agonist) and a membrane-active lytic peptide produces long-term contraception or sterilization in animals in vivo. Administering in vivo a combination of a ligand and a membrane-active lytic peptide kills cells with a receptor for the ligand. The compounds are relatively small, and are not antigenic. Lysis of gonadotropes has been observed to be very rapid (on the order of ten minutes.) Lysis of tumor cells is rapid. The two components—the ligand and the lytic peptide—may optionally be administered as a fusion peptide, or they may be administered separately, with the ligand administered slightly before the lytic peptide, to activate cells with receptors for the ligand, and thereby make those cells susceptible to lysis by the lytic peptide. The compounds may be used in gene therapy to treat malignant or non-malignant tumors, and other diseases caused by clones or populations of “normal” host cells bearing specific receptors (such as lymphocytes), because genes encoding a lytic peptide or encoding a lytic peptide/peptide hormone fusion may readily be inserted into hematopoietic stem cells or myeloid precursor cells.

    摘要翻译： 两亲性溶解肽理想地适用于配体/细胞毒素组合以特异性抑制由特定配体相互作用驱动或依赖于特定配体相互作用的细胞; 例如，诱导不育或长期避孕，或攻击肿瘤细胞，或选择性裂解病毒感染的细胞，或攻击负责自身免疫性疾病的淋巴细胞。 肽直接作用于细胞膜上，不需要内化。 管理促性腺激素释放激素（GnRH）（或GnRH激动剂）和膜活性溶解肽的组合在体内产生长期的动物避孕或灭菌。 在体内施用配体和膜活性裂解肽的组合将细胞与配体的受体杀死。 化合物相对较小，不具抗原性。 已经观察到促性腺激素的裂解非常快（十分钟左右）。肿瘤细胞裂解很快。 两种组分 - 配体和溶解肽 - 可以任选地作为融合肽施用，或者它们可以分开施用，配体在裂解肽之前略微施用，以激活配体受体的细胞，从而使得 易溶于裂解肽的细胞。 这些化合物可以用于治疗恶性或非恶性肿瘤的基因治疗，以及由具有特异性受体（如淋巴细胞）的“正常”宿主细胞的克隆或群体引起的其它疾病，因为编码溶解肽或编码溶酶体的基因 肽/肽激素融合可容易地插入到造血干细胞或骨髓前体细胞中。