公开(公告)号：US20050106652A1

公开(公告)日：2005-05-19

申请号：US10274079

申请日：2002-10-18

申请人： Richard Massey ,  Michael Powell ,  Paul Mied ,  Peter Feng ,  Leopoldo Della Ciana ,  Walter Dressick ,  Mohindar Poonian

发明人： Richard Massey ,  Michael Powell ,  Paul Mied ,  Peter Feng ,  Leopoldo Della Ciana ,  Walter Dressick ,  Mohindar Poonian

IPC分类号： C07D473/08 ,  C07F15/00 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/569 ,  G01N33/58 ,  G01N33/76 ,  C12Q1/70 ,  C12Q1/26 ,  C12Q1/37 ,  C12Q1/48

CPC分类号： G01N33/76 ,  C07D473/08 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582

摘要： Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids are provided. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety and capable of combining with the analyte of interest, exposing the resulting sample to electrochemical energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety. Further provided are methods for detecting and identifying the presence of a multiplicity of analytes in a liquid food or food homogenate. These methods comprise immersing a diagnostic reagent holder, provided with a multiplicity of reagents, into the food or food homogenate, removing the diagnostic reagent holder from the liquid food or food homogenate, and detecting and identifying the presence of a multiplicity of analytes of interest bound to the diagnostic reagent holder, thereby detecting and identifying the presence of a multiplicity of analytes of interest in the food or food homogenate. The invention further provides an enzyme immunoassay for coliform bacteria. This assay comprises inoculating a sample into a suitable medium for coliform reproduction, immobilizing coliforms present in the medium to a suitable surface, treating the surface with an antibody directed to the immobilized coliforms and detecting the presence of the immobilized coliforms immobilized to a suitable surface.

摘要翻译： 提供了对多组分液体中存在感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与标示有电化学发光化学部分的试剂接触，并能够与感兴趣的分析物结合，将所得样品暴露于电化学能并检测由电化学发光化学部分发射的电磁辐射。 还提供了用于检测和鉴定液体食物或食物匀浆中多种分析物的存在的方法。 这些方法包括将设置有多种试剂的诊断试剂保持器浸入食物或食物匀浆中，从液体食物或食物匀浆中除去诊断试剂载体，以及检测和鉴定多种感兴趣分析物的存在 到诊断试剂保持器，从而检测和鉴定食物或食物匀浆中感兴趣的多种分析物的存在。 本发明还提供了大肠杆菌的酶免疫测定法。 该测定包括将样品接种到用于大肠杆菌繁殖的合适的培养基中，将存在于培养基中的大肠杆菌固定在合适的表面上，用针对固定的大肠菌群的抗体处理该表面并检测固定在合适的表面上的固定的大肠杆菌的存在。