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    Device for magnetic immobilization of cells
    1.
    发明授权
    Device for magnetic immobilization of cells 失效
    细胞磁固定装置

    公开(公告)号：US07285412B2

    公开(公告)日：2007-10-23

    申请号：US10084063

    申请日：2002-02-28

    申请人： Rocco Casagrande ,  Evelyn Wang ,  Gregory Kirk ,  Michael Nussbaum ,  Enoch Kim ,  Aaron Raphel

    发明人： Rocco Casagrande ,  Evelyn Wang ,  Gregory Kirk ,  Michael Nussbaum ,  Enoch Kim ,  Aaron Raphel

    IPC分类号： C12M1/12 ,  C12M1/34 ,  C12N11/02 ,  C12N11/08

    CPC分类号： G01N33/54326 ,  B01J2219/00317 ,  B01J2219/00655 ,  B01J2219/00659 ,  B01J2219/00743 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2400/043 ,  C12M33/00 ,  C40B60/14 ,  G01N35/0098

    摘要： The present invention relates to an apparatus and methods that immobilize one or more cells associated with magnetic material on a substrate on which are located one or more magnetic receptacle(s). Alternatively, in another aspect the present invention, the device arrays cells associated with magnetic material on a substrate having a pattern of magnetic receptacles disposed thereon. The size of the magnetic receptacle(s) determines the number of target cells that it is capable of immobilizing. The size of the magnetic receptacle is defined by the strength of a localized magnetic field gradient. The localized magnetic field gradient may be derived from 1) permanent magnets embedded in the substrate or alternatively, the localized magnetic field gradient may be derived from an 2) external magnet whose strength is focused by objects of highly-permeable-magnetic material which create localized magnetic field gradients. The invention apparatus comprises a removable cell delivery device and a substrate, which has one or more magnetic receptacles disposed thereon.

    摘要翻译： 本发明涉及一种将与磁性材料相关联的一个或多个单元固定在位于一个或多个磁性插座上的基板上的装置和方法。 或者，在本发明的另一方面，与衬底上的磁性材料相关联的器件阵列单元具有设置在其上的磁性容器的图案。 磁性容器的尺寸决定了能够固定的靶细胞的数量。 磁性容器的尺寸由局部磁场梯度的强度限定。 局部磁场梯度可以从1）嵌入在基板中的永久磁体衍生出来，或者，局部磁场梯度可以从2）外部磁体导出，该外部磁体的强度由产生局部磁性的高透磁性材料的物体聚焦 现场梯度。 本发明的装置包括一个可拆卸的电池输送装置和一个其上设置有一个或多个磁性插座的基板。

    Cell isolation and screening device and method of using same
    3.
    发明授权
    Cell isolation and screening device and method of using same 失效
    细胞分离筛选装置及其使用方法

    公开(公告)号：US07169578B2

    公开(公告)日：2007-01-30

    申请号：US10206341

    申请日：2002-07-29

    申请人： Evelyn Wang ,  Rocco Casagrande ,  Amar Kendale ,  Enoch Kim ,  Emanuele Ostuni ,  Olivier Schueller

    发明人： Evelyn Wang ,  Rocco Casagrande ,  Amar Kendale ,  Enoch Kim ,  Emanuele Ostuni ,  Olivier Schueller

    IPC分类号： C12Q1/24

    CPC分类号： B01L3/5085 ,  B01J2219/00317 ,  B01J2219/00655 ,  B01J2219/00659 ,  B01J2219/00743 ,  B01L3/5088 ,  B01L2400/043 ,  C40B60/14 ,  G01N33/54366 ,  G01N35/0098

    摘要： The present invention provides methods and devices for screening a single cell or a small group of cells for a desired biological activity. In particular, the present invention provides for delivering cell(s) to a plurality of cell isolation regions of a cell isolation device, transferring the cell(s) to a plurality of wells of a cell expansion device and detecting the potential desired biological activity of the cell(s). Each of the cell isolation regions comprise a bioaffinity region capable of binding a single cell or a small group of cell(s). This binding of cell(s) may be accomplished through the use of bioaffinity ligands immobilized in the bioaffinity regions of the cell isolation regions. Preferably, the wells of the cell expansion device encompass a cavity that provides sufficient volume for cell proliferation.

    摘要翻译： 本发明提供用于筛选单细胞或小组细胞以获得所需生物活性的方法和装置。 特别地，本发明提供将细胞递送到细胞隔离装置的多个细胞隔离区域，将细胞转移到细胞扩增装置的多个孔中并检测潜在的所需生物活性 电池。 每个细胞分离区域包含能够结合单个细胞或小组细胞的生物亲和性区域。 细胞的这种结合可以通过使用固定在细胞分离区的生物亲和性区域中的生物亲和配体来实现。 优选地，细胞扩增装置的孔包括为细胞增殖提供足够体积的空腔。