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34 条记录 (耗时 0.034 秒)

    Cell isolation and screening device and method of using same
    1.
    发明授权
    Cell isolation and screening device and method of using same 失效
    细胞分离筛选装置及其使用方法

    公开(公告)号：US07169578B2

    公开(公告)日：2007-01-30

    申请号：US10206341

    申请日：2002-07-29

    申请人： Evelyn Wang ,  Rocco Casagrande ,  Amar Kendale ,  Enoch Kim ,  Emanuele Ostuni ,  Olivier Schueller

    发明人： Evelyn Wang ,  Rocco Casagrande ,  Amar Kendale ,  Enoch Kim ,  Emanuele Ostuni ,  Olivier Schueller

    IPC分类号： C12Q1/24

    CPC分类号： B01L3/5085 ,  B01J2219/00317 ,  B01J2219/00655 ,  B01J2219/00659 ,  B01J2219/00743 ,  B01L3/5088 ,  B01L2400/043 ,  C40B60/14 ,  G01N33/54366 ,  G01N35/0098

    摘要： The present invention provides methods and devices for screening a single cell or a small group of cells for a desired biological activity. In particular, the present invention provides for delivering cell(s) to a plurality of cell isolation regions of a cell isolation device, transferring the cell(s) to a plurality of wells of a cell expansion device and detecting the potential desired biological activity of the cell(s). Each of the cell isolation regions comprise a bioaffinity region capable of binding a single cell or a small group of cell(s). This binding of cell(s) may be accomplished through the use of bioaffinity ligands immobilized in the bioaffinity regions of the cell isolation regions. Preferably, the wells of the cell expansion device encompass a cavity that provides sufficient volume for cell proliferation.

    摘要翻译： 本发明提供用于筛选单细胞或小组细胞以获得所需生物活性的方法和装置。 特别地，本发明提供将细胞递送到细胞隔离装置的多个细胞隔离区域，将细胞转移到细胞扩增装置的多个孔中并检测潜在的所需生物活性 电池。 每个细胞分离区域包含能够结合单个细胞或小组细胞的生物亲和性区域。 细胞的这种结合可以通过使用固定在细胞分离区的生物亲和性区域中的生物亲和配体来实现。 优选地，细胞扩增装置的孔包括为细胞增殖提供足够体积的空腔。

    Device and method for monitoring leukocyte migration
    4.
    发明授权
    Device and method for monitoring leukocyte migration 失效
    用于监测白细胞迁移的装置和方法

    公开(公告)号：US07419823B2

    公开(公告)日：2008-09-02

    申请号：US10688904

    申请日：2003-10-21

    申请人： Gregory L. Kirk ,  Emanuele Ostuni ,  Enoch Kim ,  Olivier Schueller ,  Paul Sweetnam

    发明人： Gregory L. Kirk ,  Emanuele Ostuni ,  Enoch Kim ,  Olivier Schueller ,  Paul Sweetnam

    IPC分类号： C12M1/36 ,  C12M1/38 ,  C12M3/00 ,  C12M1/34 ,  C12M1/22

    CPC分类号： G01N33/5029 ,  G01N33/5047 ,  G01N33/5064

    摘要： A device for monitoring leukocyte migration is provided. The invention also provides a method of using the device to monitor leukocyte migration in the presence of physiological shear flow and therefore simulate physiological conditions of a blood vessel in vivo. The invention further provides a method of using the device to high-throughput screen a plurality of test agents. The present invention further provides a flexible assay system and numerous assays that can be used to test biological interactions and systems. Laminar flow gradients are employed that mimic gradient situations present in vivo.

    摘要翻译： 提供了一种用于监测白细胞迁移的装置。 本发明还提供了一种使用该装置在存在生理剪切流的情况下监测白细胞迁移并因此模拟体内血管生理条件的方法。 本发明还提供了一种使用该设备来高通量筛选多个测试代理的方法。 本发明进一步提供灵活的测定系统和可用于测试生物相互作用和系统的许多测定。 使用模拟体内存在的梯度情况的层流流动梯度。

    Method of making device for arraying biomolecules and for monitoring cell motility in real-time
    6.
    发明授权
    Method of making device for arraying biomolecules and for monitoring cell motility in real-time 失效
    制备用于排列生物分子和实时监测细胞运动性的装置的方法

    公开(公告)号：US07211209B2

    公开(公告)日：2007-05-01

    申请号：US10206329

    申请日：2002-07-29

    申请人： Enoch Kim ,  Gregory L. Kirk ,  Olivier Schueller ,  Emanuele Ostuni

    发明人： Enoch Kim ,  Gregory L. Kirk ,  Olivier Schueller ,  Emanuele Ostuni

    IPC分类号： B29C39/02

    CPC分类号： C12M23/12 ,  B01D2325/08 ,  B01J2219/00317 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/5085 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0829 ,  B01L2300/0887 ,  B82Y30/00 ,  C12M25/06 ,  C12M41/46 ,  C12Q1/025 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1468 ,  G01N33/54366 ,  G01N2015/1497 ,  G06K9/0014 ,  G06T7/0012 ,  G06T7/70 ,  G06T2207/30072

    摘要： The invention relates to devices, devices for arraying biomolecules, including cells, methods for arraying biomolecules, assays for monitoring cellular movement, and systems for monitoring cellular movement. The devices include a support; a first layer configured to be placed in fluid-tight contact with the support, the first layer having an upper surface and defining a pattern of micro-orifices, each micro-orifice of the pattern of micro-orifices having walls and defining a micro-region on the support when the first layer is placed in fluid-tight contact with the support such that the walls of said each micro-orifice and the micro-region on the support together define a micro-well; and a second layer configured to be placed in fluid-tight contact with the upper surface of the first layer, the second layer defining a pattern of macro-orifices, each macro-orifice of the pattern of macro-orifices having walls and defining a macro-region when the first layer is placed in fluid-tight contact with the support and the second layer is placed in fluid-tight contact with the first layer such that the walls of the macro-orifice and the macro-region together define a macro-well.

    摘要翻译： 本发明涉及用于排列生物分子的装置，包括细胞，用于排列生物分子的方法，用于监测细胞运动的测定法和用于监测细胞运动的系统。 这些装置包括一个支架; 第一层，其被配置为与所述支撑件流体密封接触，所述第一层具有上表面并限定微孔图案，所述微孔图案的每个微孔具有壁并限定微孔， 当第一层与支撑件流体密封地接触时，支撑件上的区域在支撑件上，使得所述每个微孔的壁和支撑件上的微区域一起限定微孔; 以及第二层，其被配置为与所述第一层的上表面流体密封接触，所述第二层限定宏观孔的图案，所述宏观孔的图案的每个宏观孔口具有壁并且限定宏观 当所述第一层与所述支撑件流体密封接触时，所述第二层与所述第一层流体密封接触，使得所述宏观孔和所述宏观区域的壁一起限定宏观尺寸， 好。

    Apparatus and method for handling membranes
    7.
    发明授权
    Apparatus and method for handling membranes 失效
    用于处理膜的装置和方法

    公开(公告)号：US07422651B2

    公开(公告)日：2008-09-09

    申请号：US11011558

    申请日：2004-12-14

    申请人： Aaron Raphel ,  Enoch Kim ,  Emanuele Ostuni ,  Gregory Kirk ,  Olivier Schueller

    发明人： Aaron Raphel ,  Enoch Kim ,  Emanuele Ostuni ,  Gregory Kirk ,  Olivier Schueller

    IPC分类号： C09J5/10 ,  B32B37/00 ,  B32B37/12 ,  B32B37/14 ,  C03C25/10 ,  B32B37/10 ,  B32B37/16 ,  C03C25/18

    CPC分类号： B29C41/02 ,  B29C33/424 ,  B29L2031/755 ,  Y10T428/24802 ,  Y10T428/31504

    摘要： A method for applying a pattern to a target surface includes the steps of applying a coating of membrane material over a selected portion of a substrate. The substrate imparts a pattern to the membrane material corresponding to a pattern to be applied to the target surface. A support member is positioned in contact with an outer portion of the membrane material. The support member has a higher rigidity than the membrane material. The method may also combine the steps of curing the membrane material to bond the support member to the membrane. When bonded to the membrane, the support member maintains at least a portion of the membrane in a substantially taut condition to prevent a portion of the membrane from folding onto itself. The membrane is then employed to impart the pattern to the target surface.

    摘要翻译： 将图案应用于目标表面的方法包括以下步骤：在衬底的选定部分上涂覆膜材料涂层。 基板将膜施加到与施加到目标表面的图案相对应的图案。 支撑构件定位成与膜材料的外部部分接触。 支撑构件具有比膜材料更高的刚性。 该方法还可以组合固化膜材料以将支撑构件结合到膜的步骤。 当粘合到膜时，支撑构件将膜的至少一部分保持在基本拉紧的状态，以防止膜的一部分折叠到其自身上。 然后使用膜将图案赋予目标表面。