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公开(公告)号：US20100273177A1
公开(公告)日：2010-10-28
申请号：US12517774
申请日：2007-12-10
申请人： Roger N. Piasio , Nathan Turner , Andrew Wheeler
发明人： Roger N. Piasio , Nathan Turner , Andrew Wheeler
IPC分类号： G01N33/53
CPC分类号： G01N33/56944 , G01N33/558
摘要： Provided are methods and devices for performing sensitive, rapid antigen testing of saliva, which yield sensitivity comparable to both rapid antigen tests and saliva culture.
摘要翻译： 提供了用于进行唾液的敏感，快速抗原测试的方法和装置，其产生与快速抗原测试和唾液培养相当的灵敏度。
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2.发明申请公开(公告)号：US20090011436A1
公开(公告)日：2009-01-08
申请号：US12211304
申请日：2008-09-16
申请人： Roger N. Piasio , Nathan Turner
发明人： Roger N. Piasio , Nathan Turner
IPC分类号： G01N33/53
CPC分类号： C12Q1/32 , B01J20/28033 , B01J20/281 , G01N21/78 , G01N33/523 , G01N33/54326 , G01N33/558 , G01N2333/902 , G01N2333/904 , G01N2333/986
摘要： A lateral flow chromatographic assay format for the performance of rapid enzyme-driven assays is described. A combination of components necessary to elicit a specific enzyme reaction, which are either absent from the intended sample or insufficiently present therein to permit completion of the desired reaction, are predeposited as substrate in dry form together with ingredients necessary to produce a desired color upon occurrence of the desired reaction. The strip is equipped with a sample pad placed ahead of the substrate deposit in the flowstream, to which liquid sample is applied. The sample flows from the sample pad into the substrate zone where it immediately reconstitutes the dried ingredients while also intimately mixing with them and reacting with them at the fluid front. The fluid front moves rapidly into the final “read zone” wherein the color developed is read against predetermined color standards for the desired reaction. Pretreatment pads for the sample, as needed, (e.g. a lysing pad for lysing red blood cells in whole blood) are placed in front of the sample pad in the flow path as appropriate. The assay in the format of the invention is faster and easier to perform than analogous wet chemistry assays.Specific assays for glucose-6-phosphate dehydrogenase (“G-6PD”), total serum cholesterol, β-lactamase activity and peroxidase activity are disclosed.
摘要翻译： 描述了用于快速酶驱动测定的侧流色谱测定方法。 将引起特定酶反应（其不在预期样品中或不充分存在于其中以允许完成所需反应）所需的组分的组合预先沉积为干燥形式的底物以及在发生时产生所需颜色所需的成分 的所需反应。 带材上装有样品垫，放置在流动的衬底沉积物的前面，并向其施加液体样品。 样品从样品垫流入基底区域，在那里它立即重构干燥的成分，同时与它们充分混合并在流体前面与它们反应。 流体前沿快速移动到最终的“读取区域”，其中根据预期的颜色标准读取显色的颜色用于所需的反应。 如果需要，将样品的预处理垫（例如用于裂解全血中的红细胞的裂解垫）放置在适当的流路中的样品垫的前方。 本发明形式的测定比类似的湿化学测定更快更容易进行。 公开了葡萄糖-6-磷酸脱氢酶（“G-6PD”），总血清胆固醇，β-内酰胺酶活性和过氧化物酶活性的特异性测定。
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3.发明授权Method of assaying glucose-6-phosphate dehydrogenase on a chromatographic test strip 有权在色谱测试条上测定葡萄糖-6-磷酸脱氢酶的方法公开(公告)号：US08354245B2
公开(公告)日：2013-01-15
申请号：US12211304
申请日：2008-09-16
申请人： Roger N. Piasio , Nathan Turner
发明人： Roger N. Piasio , Nathan Turner
IPC分类号： C12Q1/32
CPC分类号： C12Q1/32 , B01J20/28033 , B01J20/281 , G01N21/78 , G01N33/523 , G01N33/54326 , G01N33/558 , G01N2333/902 , G01N2333/904 , G01N2333/986
摘要： A lateral flow chromatographic assay format for the performance of rapid enzyme-driven assays is described. A combination of components necessary to elicit a specific enzyme reaction, which are either absent from the intended sample or insufficiently present therein to permit completion of the desired reaction, are predeposited as substrate in dry form together with ingredients necessary to produce a desired color upon occurrence of the desired reaction. The strip is equipped with a sample pad placed ahead of the substrate deposit in the flowstream, to which liquid sample is applied. The sample flows from the sample pad into the substrate zone where it immediately reconstitutes the dried ingredients while also intimately mixing with them and reacting with them at the fluid front. The fluid front moves rapidly into the final “read zone” wherein the color developed is read against predetermined color standards for the desired reaction. Pretreatment pads for the sample, as needed, (e.g. a lysing pad for lysing red blood cells in whole blood) are placed in front of the sample pad in the flow path as appropriate. The assay in the format of the invention is faster and easier to perform than analogous wet chemistry assays.
摘要翻译： 描述了用于快速酶驱动测定的侧流色谱测定方法。 将引起特定酶反应（其不在预期样品中或不充分存在于其中以允许完成所需反应）所需的组分的组合预先沉积为干燥形式的底物以及在发生时产生所需颜色所需的成分 的所需反应。 带材上装有样品垫，放置在流动的衬底沉积物的前面，并向其施加液体样品。 样品从样品垫流入基底区域，在那里它立即重构干燥的成分，同时与它们充分混合并在流体前面与它们反应。 流体前沿快速移动到最终读取区域，其中显色的颜色针对期望的反应的预定颜色标准读取。 如果需要，将样品的预处理垫（例如用于裂解全血中的红细胞的裂解垫）放置在适当的流路中的样品垫的前方。 本发明形式的测定比类似的湿化学测定更快更容易进行。
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4.发明授权公开(公告)号：US07425302B2
公开(公告)日：2008-09-16
申请号：US10784155
申请日：2004-02-24
申请人： Roger N. Piasio , Nathan Turner
发明人： Roger N. Piasio , Nathan Turner
IPC分类号： G01N21/00
CPC分类号： C12Q1/32 , B01J20/28033 , B01J20/281 , G01N21/78 , G01N33/523 , G01N33/54326 , G01N33/558 , G01N2333/902 , G01N2333/904 , G01N2333/986
摘要： A lateral flow chromatographic assay format for the performance of rapid enzyme-driven assays is described. A combination of components necessary to elicit a specific enzyme reaction, which are either absent from the intended sample or insufficiently present therein to permit completion of the desired reaction, are predeposited as substrate in dry form together with ingredients necessary to produce a desired color upon occurrence of the desired reaction. The strip is equipped with a sample pad placed ahead of the substrate deposit in the flowstream, to which liquid sample is applied. The sample flows from the sample pad into the substrate zone where it immediately reconstitutes the dried ingredients while also intimately mixing with them and reacting with them at the fluid front. The fluid front moves rapidly into the final “read zone” wherein the color developed is read against predetermined color standards for the desired reaction. Pretreatment pads for the sample, as needed, (e.g. a lysing pad for lysing red blood cells in whole blood) are placed in front of the sample pad in the flow path as appropriate. The assay in the format of the invention is faster and easier to perform than analogous wet chemistry assays.Specific assays for glucose-6-phosphate dehydrogenase (“G-6PD”), total serum cholesterol, β-lactamase activity and peroxidase activity are disclosed.
摘要翻译： 描述了用于快速酶驱动测定的侧流色谱测定方法。 将引起特定酶反应（其不在预期样品中或不充分存在于其中以允许完成所需反应）所需的组分的组合预先沉积为干燥形式的底物以及在发生时产生所需颜色所需的成分 的所需反应。 带材上装有样品垫，放置在流动的衬底沉积物的前面，并向其施加液体样品。 样品从样品垫流入基底区域，在那里它立即重构干燥的成分，同时与它们充分混合并在流体前面与它们反应。 流体前沿快速移动到最终的“读取区域”，其中根据预期的颜色标准读取显色的颜色用于所需的反应。 如果需要，将样品的预处理垫（例如用于裂解全血中的红细胞的裂解垫）放置在适当的流路中的样品垫的前方。 本发明形式的测定比类似的湿化学测定更快更容易进行。 公开了葡萄糖-6-磷酸脱氢酶（“G-6PD”），总血清胆固醇，β-内酰胺酶活性和过氧化物酶活性的特异性测定。
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5.发明授权公开(公告)号：US07018849B2
公开(公告)日：2006-03-28
申请号：US10044920
申请日：2002-01-15
申请人： Roger N. Piasio , Nathan Turner
发明人： Roger N. Piasio , Nathan Turner
IPC分类号： G01N33/553
CPC分类号： G01N33/56983 , G01N33/54326 , G01N33/558 , G01N2446/00 , G01N2446/20 , G01N2446/80 , Y10S435/805 , Y10S435/81 , Y10S435/97 , Y10S436/806 , Y10T436/25375
摘要： Superparamagnetic (“SPM”) subunits of 1–30 nm average mean diameter (e.g. ferro fluid) subparticles are treated with a magnetically noninterfering substance capable of coating and covering them (e.g, BSA) and they spontaneously form agglomerates of about 100 nm to about 450 nm or higher average mean diameter and are then used to form complexes with target biological ligands such as viruses, contained in large volumes of liquid. The complexes are subjected to the gradient intensity of a strong magnetic field, and excess liquid is removed, where upon an immunochromatographic assay is conducted to determine the identity and/or amount of target ligand present, in which operation SPM particles that bonded to the ligand function as tags for ligand detection.
摘要翻译： 使用能够涂覆和覆盖它们的磁性非干扰性物质（例如BSA）处理1-30nm平均平均直径（例如铁流体）子粒子的超顺磁性（“SPM”）亚基，并且它们自发形成约100nm至约 450nm或更高的平均直径，然后用于与包含在大体积液体中的目标生物配体如病毒形成复合物。 络合物经受强磁场的梯度强度，并除去过量的液体，其中进行免疫色谱测定以确定存在的靶配体的同一性和/或量，其中与配体结合的操作SPM颗粒 作为配体检测的标签。
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6.发明授权公开(公告)号：US07358099B2
公开(公告)日：2008-04-15
申请号：US11295486
申请日：2005-12-07
申请人： Roger N. Piasio , Nathan Turner
发明人： Roger N. Piasio , Nathan Turner
IPC分类号： G01N33/553 , G01N33/558
CPC分类号： G01N33/56983 , G01N33/54326 , G01N33/558 , G01N2446/00 , G01N2446/20 , G01N2446/80 , Y10S435/805 , Y10S435/81 , Y10S435/97 , Y10S436/806 , Y10T436/25375
摘要： Superparamagnetic (“SPM”) subunits of 1–30 nm average mean diameter (e.g. ferro fluid) subparticles are treated with a magnetically noninterfering substance capable of coating and covering them (e.g, BSA) and they spontaneously form agglomerates of about 100 nm to about 450 nm or higher average mean diameter and are then used to form complexes with target biological ligands such as viruses, contained in large volumes of liquid. The complexes are subjected to the gradient intensity of a strong magnetic field, and excess liquid is removed, where upon an immunochromatographic assay is conducted to determine the identity and/or amount of target ligand present, in which operation SPM particles that bonded to the ligand function as tags for ligand detection.
摘要翻译： 使用能够涂覆和覆盖它们的磁性非干扰性物质（例如BSA）处理1-30nm平均平均直径（例如铁流体）子粒子的超顺磁性（“SPM”）亚基，并且它们自发形成约100nm至约 450nm或更高的平均直径，然后用于与包含在大体积液体中的目标生物配体如病毒形成复合物。 络合物经受强磁场的梯度强度，并除去过量的液体，其中进行免疫色谱测定以确定存在的靶配体的同一性和/或量，其中与配体结合的操作SPM颗粒 作为配体检测的标签。
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公开(公告)号：US20110318755A1
公开(公告)日：2011-12-29
申请号：US13020485
申请日：2011-02-03
CPC分类号： G01N33/558 , G01N21/03 , G01N21/11 , G01N21/8483 , G01N33/54366 , G01N2021/0328
摘要： The invention relates to analysis of samples (for example, blood, urine, saliva, or swab). Some embodiments include a diagnostic testing platform. The platform may permit the analysis of a plurality of different samples with no or only minor modifications made to the platform. This platform may also reduce the number of steps performed by a user, and offer increased sensitivity and precision. The platform may be integrated with a low-cost instrument and provide an accurate digital analysis of the reaction.
摘要翻译： 本发明涉及样品（例如血液，尿液，唾液或拭子）的分析。 一些实施例包括诊断测试平台。 该平台可以允许分析多个不同的样本，没有或仅对平台进行微小的修改。 该平台还可以减少用户执行的步骤数量，并提供增加的灵敏度和精度。 该平台可与低成本仪器集成，并提供准确的反应数字分析。
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8.发明申请Method for the Reduction of Biological Sampling Errors by Means of Image Processing 审中-公开通过图像处理减少生物抽样误差的方法公开(公告)号：US20120208202A1
公开(公告)日：2012-08-16
申请号：US13236038
申请日：2011-09-19
IPC分类号： G01N33/53
CPC分类号： G01N33/543
摘要： The present invention relates to methods and devices for reducing biological sampling errors by means of image processing. Image processing techniques are used to determine the volume of sample added to a device, such as a diagnostic test, and to correct for user error in sampling techniques.
摘要翻译： 本发明涉及通过图像处理减少生物抽样误差的方法和装置。 图像处理技术用于确定添加到设备的样本的体积，如诊断测试，并纠正采样技术中的用户错误。
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公开(公告)号：US5939252A
公开(公告)日：1999-08-17
申请号：US853760
申请日：1997-05-09
申请人： Donald J. Lennon , Roger N. Piasio
发明人： Donald J. Lennon , Roger N. Piasio
CPC分类号： G01N33/54386 , Y10S435/97 , Y10S435/975 , Y10S436/807 , Y10S436/808 , Y10S436/825
摘要： An assay device for detection or determination of an analyte in a sample uses either removably attachable components or hinged panels to provide greater flexibility and reduce manufacturing and storage costs. In one embodiment of the device, the device comprises: (1) a first opposable component including:(a) a first panel; (b) a second panel mounted on the first panel generally parallel to the first panel with space between the first and second panel, the second panel having an opening forming a first receptacle for a sample collection device; and (c) a second receptacle for a test strip formed by the first panel and the second panel; and (2) a second opposable component hingedly attached to the first opposable component. In this device, the first and second opposable components can be brought into operable contact so that fluid is expressed from the sample collection device and applied to the test strip for detection or determination of an analyte by a test performed on the test strip. Other embodiments of devices are included, as well as test kits and method of use of the devices.
摘要翻译： 用于检测或确定样品中分析物的测定装置使用可拆卸地附接的部件或铰接板来提供更大的灵活性并降低制造和存储成本。 在该设备的一个实施例中，该装置包括：（1）第一可反对组件，包括：（a）第一面板; （b）安装在所述第一面板上的大致平行于所述第一面板的第二面板，所述第一面板具有位于所述第一面板与所述第二面板之间的空间，所述第二面板具有形成用于样品收集装置的第一容器的开口; 和（c）由第一面板和第二面板形成的用于测试条的第二容器; 和（2）铰接地附接到第一可反涂组分的第二可反涂组分。 在该装置中，可以使第一和第二可反映部件可操作地接触，使得流体从样品收集装置表达并施加到测试条上，以通过在试纸上进行的测试来检测或确定分析物。 包括设备的其他实施例，以及测试套件和设备的使用方法。
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公开(公告)号：US4098876A
公开(公告)日：1978-07-04
申请号：US735740
申请日：1976-10-26
IPC分类号： G01N33/538 , G01N33/543 , G01N33/552 , G01N33/78 , G01N33/16 , A61K43/00
CPC分类号： G01N33/552 , G01N33/538 , G01N33/54306 , G01N33/78 , Y10S436/82
摘要： Method of determining the presence and/or concentration of a polyvalent antigenic substance in a fluid. The method comprises the steps of incubating the fluid with labeled antibodies to the substance to form a first labeled immunochemical complex and then incubating that complex with immobilized antibodies to the substance to form a second labeled complex which is separated from the incubation medium. The amount of label in the second complex provides a means for detecting and/or quantitating the substance in the fluid. By reversing the sequence of incubation steps in a "two-site" or sandwich assay, greater sensitivity is achieved and an intermediate washing step is eliminated.
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