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公开(公告)号：US07989187B2
公开(公告)日：2011-08-02
申请号：US10520220
申请日：2003-07-03
摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.
摘要翻译： 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增强所需产物的产生的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。
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公开(公告)号：US07291489B2
公开(公告)日：2007-11-06
申请号：US10614333
申请日：2003-07-03
摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.
摘要翻译： 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增强所需产物的产生的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。
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公开(公告)号：US20080166777A1
公开(公告)日：2008-07-10
申请号：US11879143
申请日：2007-07-16
IPC分类号： C12P13/02 , C07C261/00 , C12N1/20
CPC分类号： C12N9/0006 , C12N9/1014 , C12N9/1022 , C12N9/88 , C12N9/93 , C12P13/02 , C12Y201/02011 , C12Y401/01011 , C12Y603/02001
摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate by enzymes that contribute to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.
摘要翻译： 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加关键中间体水平，通过有助于其合成的酶的酮托品来提高所需产物的生产的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。
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公开(公告)号：US07244593B2
公开(公告)日：2007-07-17
申请号：US10466641
申请日：2002-01-18
IPC分类号： C12P13/04
CPC分类号： C12N9/0006 , C12N9/1014 , C12N9/1022 , C12N9/88 , C12N9/93 , C12P13/02 , C12Y201/02011 , C12Y401/01011 , C12Y603/02001
摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate by enzymes that contribute to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.
摘要翻译： 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加关键中间体水平，通过有助于其合成的酶的酮托品来提高所需产物的生产的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。
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公开(公告)号：US08232081B2
公开(公告)日：2012-07-31
申请号：US10984449
申请日：2004-11-08
摘要： The present invention features methods of producing panto-compounds (e.g., pantothenate) using microorganisms in which the pantothenate biosynthetic pathway and/or the isoleucine-valine biosynthetic pathway and/or the coenzymeA biosynthetic pathway has been manipulated. Methods featuring ketopantoate reductase overexpressing microorganisms as well as aspartate α-decarboxylase overexpressing microorganisms are provided. Methods of producing panto-compounds in a precursor-independent manner and in high yield are described. Recombinant microorganisms, vectors, isolated nucleic acid molecules, genes and gene products useful in practicing the above methodologies are also provided. The present invention also features a previously unidentified microbial pantothenate kinase gene, coaX, as well as methods of producing panto-compounds utilizing microorganisms having modified pantothenate kinase activity. Recombinant microorganisms, vectors, isolated coaX nucleic acid molecules and purified CoaX proteins are featured. Also featured are methods for identifying pantothenate kinase modulators utilizing the recombinant microorganisms and/or purified CoaX proteins of the present invention.
摘要翻译： 本发明的特征在于使用其中泛酸生物合成途径和/或异亮氨酸 - 缬氨酸生物合成途径和/或辅酶A生物合成途径已经被操纵的微生物来生产泛化合物（例如泛酸盐）的方法。 提供了酮过氧还原酶过表达微生物以及天冬氨酸α-脱羧酶过表达微生物的方法。 描述了以前体非依赖性方式和高产率生产泛化合物的方法。 还提供了用于实践上述方法的重组微生物，载体，分离的核酸分子，基因和基因产物。 本发明还具有以前未鉴定的微生物泛酸激酶基因coaX，以及利用具有改良的泛酸激酶活性的微生物产生泛化合物的方法。 重组微生物，载体，分离的coaX核酸分子和纯化的CoaX蛋白。 还特征在于利用本发明的重组微生物和/或纯化的CoaX蛋白鉴定泛酸激酶调节剂的方法。
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公开(公告)号：US20090298136A1
公开(公告)日：2009-12-03
申请号：US11988962
申请日：2006-07-18
申请人： Oskar Zelder , Stefan Haefner , Corinna Klopprogge , Hartwig Schroder , Andrea Herold , Thomas A. Patterson , Theron Hermann , R. Rogers Yocum , Mark K. William , Janice G. Pero
发明人： Oskar Zelder , Stefan Haefner , Corinna Klopprogge , Hartwig Schroder , Andrea Herold , Thomas A. Patterson , Theron Hermann , R. Rogers Yocum , Mark K. William , Janice G. Pero
摘要： This invention relates to methionine producing recombinant microorganisms. Specifically, this invention relates to recombinant strains of Corynebacterium that produce increased levels of methionine compared to their wild-type counterparts and further to methods of generating such microorganisms.
摘要翻译： 本发明涉及产生甲硫氨酸的重组微生物。 具体而言，本发明涉及棒状杆菌的重组菌株，其与野生型对应物相比产生较高水平的甲硫氨酸，并且还涉及产生这种微生物的方法。
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公开(公告)号：US07220561B2
公开(公告)日：2007-05-22
申请号：US10466717
申请日：2002-01-19
申请人： Theron Hermann , Thomas A. Patterson , Janice G. Pero , R. Roger Yocum , Kai-Uwe Baldenius , Christine Beck
发明人： Theron Hermann , Thomas A. Patterson , Janice G. Pero , R. Roger Yocum , Kai-Uwe Baldenius , Christine Beck
CPC分类号： C12P13/04 , C12N9/1022 , C12P13/02
摘要： The present invention features improved methods for producing pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities. In particular, the invention features methods for reducing byproduct formation and increasing yields and purity of desired product. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.
摘要翻译： 本发明的特征在于利用具有改良泛酸生物合成酶活性的微生物生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于减少副产物形成和提高所需产物的产率和纯度的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。
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8.发明授权公开(公告)号：US07091000B2
公开(公告)日：2006-08-15
申请号：US10361522
申请日：2003-02-10
申请人： John B. Perkins , Alan Sloma , Janice G. Pero , Randolph T. Hatch , Theron Hermann , Thomas Erdenberger
发明人： John B. Perkins , Alan Sloma , Janice G. Pero , Randolph T. Hatch , Theron Hermann , Thomas Erdenberger
摘要： Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e.g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.
摘要翻译： 用于过量产生核黄素的载体和重组细菌，其中过量产生核黄素生物合成蛋白的核酸被引入宿主生物体的染色体，例如。 在多个站点和每个站点的多个副本。 使用具有至少五个基因的rib操纵子来制备这样的重组细菌。
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公开(公告)号：US06551813B1
公开(公告)日：2003-04-22
申请号：US09306615
申请日：1999-05-06
申请人： John B. Perkins , Alan Sloma , Janice G. Pero , Randolph T. Hatch , Theron Hermann , Thomas Erdenberger
发明人： John B. Perkins , Alan Sloma , Janice G. Pero , Randolph T. Hatch , Theron Hermann , Thomas Erdenberger
IPC分类号： C12P2500
摘要： Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e.g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.
摘要翻译： 用于过量产生核黄素的载体和重组细菌，其中过量产生核黄素生物合成蛋白的核酸被引入宿主生物体的染色体，例如。 在多个站点和每个站点的多个副本。 使用具有至少五个基因的rib操纵子来制备这样的重组细菌。
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公开(公告)号：US5925538A
公开(公告)日：1999-07-20
申请号：US138775
申请日：1998-08-24
申请人： John B. Perkins , Alan Sloma , Janice G. Pero , Randolph T. Hatch , Theron Hermann , Thomas Erdenberger
发明人： John B. Perkins , Alan Sloma , Janice G. Pero , Randolph T. Hatch , Theron Hermann , Thomas Erdenberger
摘要： Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e.g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.
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