公开(公告)号：US20050118230A1

公开(公告)日：2005-06-02

申请号：US10971544

申请日：2004-10-22

申请人： Ronald Hill ,  Richard Klann ,  Francis Lamberti

发明人： Ronald Hill ,  Richard Klann ,  Francis Lamberti

IPC分类号： A61K35/32 ,  A61K38/18 ,  A61K38/19 ,  A61K38/30 ,  A61K38/38 ,  A61K38/39 ,  A61K45/00 ,  A61L26/00 ,  A61L27/26 ,  A61L27/48 ,  A61L27/52

CPC分类号： A61L27/52 ,  A61K35/32 ,  A61K38/014 ,  A61K38/1875 ,  A61L26/0052 ,  A61L26/0057 ,  A61L26/008 ,  A61L27/26 ,  A61L27/3608 ,  A61L27/3645 ,  A61L27/3834 ,  A61L27/48 ,  A61L2430/10 ,  C08L5/02 ,  A61K2300/00 ,  C08L89/06

摘要： Connective tissue regenerative compositions and methods of repairing and regenerating connective tissue using such compositions are provided. The compositions generally comprise a bioactive hydrogel matrix comprising a polypeptide, such as gelatin, and a long chain carbohydrate, such as dextran. The hydrogel matrix may further include polar amino acids, as well as additional beneficial additives. Advantageously, the compositions include further components, such as osteoinductive or osteoconductive materials, medicaments, stem or progenitor cells, and three-dimensional structural frameworks. The compositions are useful for regenerating connective tissue, and can be administered to an area having injury to, or a loss of, connective tissue, such as bone, cartilage, tendon, and ligament.

摘要翻译： 提供结缔组织再生组合物和使用这种组合物修复和再生结缔组织的方法。 组合物通常包含包含多肽（如明胶）和长链碳水化合物如葡聚糖的生物活性水凝胶基质。 水凝胶基质可以进一步包括极性氨基酸，以及其它有益的添加剂。 有利地，组合物包括其它组分，例如骨诱导材料或骨传导材料，药物，茎或祖细胞和三维结构框架。 所述组合物可用于再生结缔组织，并且可以施用于结缔组织如骨，软骨，腱和韧带的损伤或丧失的区域。

公开(公告)号：US5827707A

公开(公告)日：1998-10-27

申请号：US484778

申请日：1995-06-07

申请人： Francis Lamberti

发明人： Francis Lamberti

IPC分类号： A61K9/16 ,  A61K35/12 ,  A61K35/39 ,  A61L27/38 ,  A61K9/50 ,  B01J13/20 ,  C12N5/06

CPC分类号： A61L27/3804 ,  A61K35/39 ,  A61K9/1652 ,  A61K9/1694 ,  A61L27/3683 ,  Y10S514/866

摘要： The present invention provides methods and a device for producing minimal volume capsules containing viable cells or cellular aggregates. The methods and device use a two-phase aqueous emulsion system to form a dispersion of liquid capsule-forming materials in a continuous liquid phase to which is added a suspension of biological material. Alternatively, the biological material can be added to one or the other of the liquid phases. The composition of this emulsion is adjusted to promote the thermodynamically-driven process of particle engulfment by the dispersed droplets of liquid capsule-forming materials. Subsequently, the droplets engulf the biological material to form a liquid film surrounding the tissue and are converted to solid form, resulting in encapsulation of the biological material in minimum volume capsules.

摘要翻译： 本发明提供了用于生产包含活细胞或细胞聚集体的最小体积胶囊的方法和装置。 所述方法和装置使用两相水乳液体系以形成液体胶囊形成材料在连续液相中的分散体，向其中加入生物材料的悬浮液。 或者，生物材料可以添加到一个或另一个液相中。 调整该乳液的组成以促进通过液体胶囊形成材料的分散液滴的颗粒吞噬的热力学驱动过程。 随后，液滴吞噬生物材料以形成围绕组织的液膜并转化为固体形式，导致将生物材料包封在最小体积的胶囊中。

公开(公告)号：US06489164B1

公开(公告)日：2002-12-03

申请号：US09131762

申请日：1998-08-10

申请人： Brad Gray ,  Monty Kahn Baird ,  Francis Lamberti

发明人： Brad Gray ,  Monty Kahn Baird ,  Francis Lamberti

IPC分类号： C12N500

CPC分类号： A61K35/39

摘要： A method for isolating specific viable cell types from surrounding organ tissue is provided. The method entails the use of sonication in conjunction with tissue dissociating agents to free the cells of interest. A specific application of the method is the isolation of the insulin producing tissue of the pancreas, the islets of Langerhans. The method results in a high yield of islets that maintain a high level of viability.

摘要翻译： 提供了从周围器官组织分离特定活细胞类型的方法。 该方法需要与组织解离剂结合使用超声处理以释放感兴趣的细胞。 该方法的具体应用是分离胰岛素胰岛素组织，胰岛胰岛素。 该方法导致高产量的胰岛，其保持高水平的活力。

公开(公告)号：US5879939A

公开(公告)日：1999-03-09

申请号：US442587

申请日：1995-05-17

申请人： Brad Gray ,  Monty Kahn Baird ,  Francis Lamberti

发明人： Brad Gray ,  Monty Kahn Baird ,  Francis Lamberti

IPC分类号： C12N5/06 ,  A61K35/39 ,  C12N5/02 ,  C12N5/00

CPC分类号： A61K35/39

摘要： A method for isolating specific viable cell types from surrounding organ tissue is provided. The method entails the use of sonication in conjunction with tissue dissociating agents to free the cells of interest. A specific application of the method is the isolation of the insulin producing tissue of the pancreas, the islets of Langerhans. The method results in a high yield of islets that maintain a high level of viability.