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1.发明申请公开(公告)号：US20110065110A1
公开(公告)日：2011-03-17
申请号：US12849455
申请日：2010-08-03
申请人： Roxana Isac , Frederic Marc
发明人： Roxana Isac , Frederic Marc
IPC分类号： C12Q1/68
CPC分类号： C12N15/1003 , C12N15/1017
摘要： The invention relates to a method for the extraction and detection of nucleic acids on a membrane, making it possible to identify the microorganisms present in low concentration in a liquid or gaseous medium, as well as a novel lysis composition comprising guanidium chloride and between 0.1 and 1% N-Lauroyl-Sarcosine (NLS) making it possible to implement this method.
摘要翻译： 本发明涉及一种用于提取和检测膜上的核酸的方法，使得可以鉴定液体或气体介质中低浓度存在的微生物以及包含氯化胍的新型裂解组合物和0.1至 1％的N-月桂酰肌氨酸（NLS）可以实现这种方法。
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2.发明申请公开(公告)号：US20100305312A1
公开(公告)日：2010-12-02
申请号：US12849463
申请日：2010-08-03
申请人： Roxana Isac , Frederic Marc
发明人： Roxana Isac , Frederic Marc
IPC分类号： C07H21/00
CPC分类号： C12N15/1003 , C12N15/1017
摘要： The invention relates to a method for the extraction and detection of nucleic acids on a membrane, making it possible to identify the microorganisms present in low concentration in a liquid or gaseous medium, as well as a novel lysis composition comprising guanidium chloride and between 0.1 and 1% N-Lauroyl-Sarcosine (NLS) making it possible to implement this method.
摘要翻译： 本发明涉及一种用于提取和检测膜上的核酸的方法，使得可以鉴定液体或气体介质中低浓度存在的微生物以及包含氯化胍的新型裂解组合物和0.1至 1％的N-月桂酰肌氨酸（NLS）可以实现这种方法。
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3.发明申请公开(公告)号：US20090226910A1
公开(公告)日：2009-09-10
申请号：US12321297
申请日：2009-01-20
申请人： Roxana Isac , Frederic Marc
发明人： Roxana Isac , Frederic Marc
CPC分类号： C12N15/1003 , C12N15/1017
摘要： The invention relates to a method for the extraction and detection of nucleic acids on a membrane, making it possible to identify the microorganisms present in low concentration in a liquid or gaseous medium, as well as a novel lysis composition comprising guanidium chloride and between 0.1 and 1% N-Lauroyl-Sarcosine (NLS) making it possible to implement this method.
摘要翻译： 本发明涉及一种用于提取和检测膜上的核酸的方法，使得可以鉴定液体或气体介质中低浓度存在的微生物以及包含氯化胍的新型裂解组合物和0.1至 1％的N-月桂酰肌氨酸（NLS）可以实现这种方法。
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公开(公告)号：US20120315663A1
公开(公告)日：2012-12-13
申请号：US13579996
申请日：2011-02-02
申请人： Frederic Marc , Luc Felden , Gael Waiche , Jean-Jacques Richert
发明人： Frederic Marc , Luc Felden , Gael Waiche , Jean-Jacques Richert
CPC分类号： B01L9/52 , B01L2200/026 , B01L2300/0681 , B01L2300/0822 , G01N1/4077 , G01N2001/4088
摘要： A device (1, 100) for separating a membrane (81, 91) to analyze from a support (83, 93), the membrane being mounted in a frame (82, 92), itself mounted on a support, having a body (10) comprising a longitudinal recess (11, 110, 120) for translational reception of the assembly constituted by the frame mounted on the support, the recess having a first end (12) and a second end (13)and longitudinal sides(14A, 14B, 111, 121) facing each other between said two ends, the device having bearing means (30, 112, 122) for a first member of the frame/support group between the first and the second end of the recess, which are adapted to serve as a bearing for the first member of the frame/support group once the assembly is disposed in the recess, each of the sides having a ramp (15A, 15B, 112, 122) adapted to engage a gripping member (84, 73) of the second member of the frame/support group when the assembly is translationally moved from the first to the second end, said ramps being adapted to separate the support from the frame during said translational movement by bearing on said gripping member of the second member of the frame/support group, the first member of the frame/support group then bearing on the bearing means, such that the frame and the support are separated once the translational movement has arrived at the second end.
摘要翻译： 一种用于分离膜（81,91）以从支撑件（83,93）分析的装置（100,100），所述膜被安装在框架（82,92）中，框架（82,92）本身安装在具有主体的支撑件上 10），包括用于平移接收由安装在支撑件上的框架组成的组件的纵向凹部（11,110,120），该凹部具有第一端部（12）和第二端部（13）以及纵向侧面（14A， 14B，111,121）在所述两端之间彼此面对，所述装置具有用于框架/支撑组的第一构件的支撑装置（30,112,122），其在凹部的第一和第二端之间适应 一旦将组件设置在凹部中，每个侧面具有适于接合夹紧构件（84,73）的斜面（15A，15B，112,122）的框架/支撑组件的第一构件的轴承 ）当所述组件从所述第一端平移到所述第二端时所述框架/支撑组的第二构件，所述斜面适于分离 在所述平移运动期间通过支撑在框架/支撑组的第二构件的所述夹持构件上的框架处的支撑件，框架/支撑组的第一构件然后承载在轴承装置上，使得框架和支撑件 一旦平移运动到达第二端就分离。
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5.发明申请公开(公告)号：US20110059433A1
公开(公告)日：2011-03-10
申请号：US11919474
申请日：2006-04-26
申请人： Frederic Marc , Serge Ohresser
发明人： Frederic Marc , Serge Ohresser
CPC分类号： C12Q1/6888 , B01D61/18 , B01L3/502 , B01L2300/0618 , B01L2300/0681 , C12Q1/04 , C12Q2600/16
摘要： The present invention relates to a method for the specific detection on a filter of one or more microorganisms present in a fluid, characterized in that it comprises the following steps: a) contacting the microorganisms present in the fluid or on the surface with the filter; b) amplifying specifically the nucleic acids from the microorganism or microorganisms present on the filter, in an isothermal manner, in order to obtain amplification products, c) detecting the amplification products. The invention also relates to a device, a kit and oligonucleotides suitable for the implementation of this method.
摘要翻译： 本发明涉及用于特定检测存在于流体中的一种或多种微生物的过滤器的方法，其特征在于其包括以下步骤：a）使流体或表面上存在的微生物与过滤器接触; b）以等温方式特异性扩增存在于过滤器上的微生物或微生物的核酸，以获得扩增产物，c）检测扩增产物。 本发明还涉及适用于实施该方法的装置，试剂盒和寡核苷酸。
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6.发明申请Growth Medium For The Detection Of Microorganisms By Fluorescence Allying A Fluorogenic Substrate And A PH-Sensitive Fluorophore 有权通过荧光检测微生物的生长培养基荧光基质和PH敏感性荧光团公开(公告)号：US20120301916A1
公开(公告)日：2012-11-29
申请号：US13522797
申请日：2011-01-19
申请人： Frederic Marc
发明人： Frederic Marc
IPC分类号： G01N21/64
CPC分类号： C12Q1/045
摘要： The invention relates to a growth medium allying a fluorogenic substrate and a pH-sensitive fluorophore, in particular the combination of 4-methylumbelliferone (4-MU) and derivatives of fluorescein. This growth medium is used for the detection by fluorescence of microorganisms by coupling the fluorescence measurements relating to the pH-sensitive fluorophor and the fluorescence measurements relating to the activation of the fluorogenic substrate(s) by the microorganisms.
摘要翻译： 本发明涉及一种赋予荧光底物和pH敏感荧光团的生长培养基，特别是4-甲基伞形酮（4-MU）和荧光素衍生物的组合。 该生长培养基用于通过将与pH敏感荧光荧光素相关的荧光测量和与微生物对荧光底物的活化相关的荧光测量结合来检测微生物的荧光。
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公开(公告)号：US08148081B2
公开(公告)日：2012-04-03
申请号：US12692741
申请日：2010-01-25
申请人： Sebastien Ribault , Frederic Marc
发明人： Sebastien Ribault , Frederic Marc
CPC分类号： C12Q1/04 , C12Q1/6806 , C12Q2527/125
摘要： The present invention relates to a method using a composition for permeabilizing microorganism walls for counting and detecting in a targeted manner the microorganisms on a membrane. The invention also relates to a kit and to probes that are suitable for carrying out the method.
摘要翻译： 本发明涉及一种使用微生物壁透化组合物的方法，用于计数并以目标方式检测膜上的微生物。 本发明还涉及适用于实施该方法的试剂盒和探针。
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8.发明申请Composition for increasing microorganism wall permeability and method for detecting said microorganisms on a membrane 审中-公开用于增加微生物壁渗透性的组合物和用于在膜上检测所述微生物的方法公开(公告)号：US20090197247A1
公开(公告)日：2009-08-06
申请号：US11638745
申请日：2006-12-14
申请人： Sebastien Ribault , Frederic Marc , Manjula Aysola
发明人： Sebastien Ribault , Frederic Marc , Manjula Aysola
CPC分类号： C12Q1/04 , C12Q1/6806 , C12Q2527/125
摘要： The present invention relates to a composition for permeabilizing microorganism walls, comprising the combination of polyethyleneimine (PEI) with at least one alcohol, and also to a method using said composition for counting and detecting in a targeted manner the microorganisms on a membrane. The invention also relates to a kit and to probes that are suitable for carrying out said method.
摘要翻译： 本发明涉及用于渗透微生物壁的组合物，其包含聚乙烯亚胺（PEI）与至少一种醇的组合，以及使用所述组合物以靶向方式计数和检测膜上的微生物的方法。 本发明还涉及适用于实施所述方法的试剂盒和探针。
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9.发明申请公开(公告)号：US20110318744A1
公开(公告)日：2011-12-29
申请号：US13195336
申请日：2011-08-01
申请人： Frederic Marc , Serge Ohresser
发明人： Frederic Marc , Serge Ohresser
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6888 , B01D61/18 , B01L3/502 , B01L2300/0618 , B01L2300/0681 , C12Q1/04 , C12Q2600/16
摘要： The present invention relates to a method for the specific detection on a filter of one or more microorganisms present in a fluid, characterized in that it comprises the following steps: a) contacting the microorganisms present in the fluid or on the surface with the filter; b) amplifying specifically the nucleic acids from the microorganism or microorganisms present on the filter, in an isothermal manner, in order to obtain amplification products, c) detecting the amplification products. The invention also relates to a device, a kit and oligonucleotides suitable for the implementation of this method.
摘要翻译： 本发明涉及用于特定检测存在于流体中的一种或多种微生物的过滤器的方法，其特征在于其包括以下步骤：a）使流体或表面上存在的微生物与过滤器接触; b）以等温方式特异性扩增存在于过滤器上的微生物或微生物的核酸，以获得扩增产物，c）检测扩增产物。 本发明还涉及适用于实施该方法的装置，试剂盒和寡核苷酸。
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10.发明授权Growth medium for the detection of microorganisms by fluorescence allying a fluorogenic substrate and a pH-sensitive fluorophore 有权用于通过荧光检测微生物的生长培养基，其荧光底物和pH敏感的荧光团公开(公告)号：US09080198B2
公开(公告)日：2015-07-14
申请号：US13522797
申请日：2011-01-19
申请人： Frederic Marc
发明人： Frederic Marc
CPC分类号： C12Q1/045
摘要： The invention relates to a growth medium allying a fluorogenic substrate and a pH-sensitive fluorophore, in particular the combination of 4-methylumbelliferone (4-MU) and derivatives of fluorescein. This growth medium is used for the detection by fluorescence of microorganisms by coupling the fluorescence measurements relating to the pH-sensitive fluorophor and the fluorescence measurements relating to the activation of the fluorogenic substrate(s) by the microorganisms.
摘要翻译： 本发明涉及一种赋予荧光底物和pH敏感荧光团的生长培养基，特别是4-甲基伞形酮（4-MU）和荧光素衍生物的组合。 该生长培养基用于通过将与pH敏感荧光荧光素相关的荧光测量和与微生物对荧光底物的活化相关的荧光测量结合来检测微生物的荧光。
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