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    Vitro system for determining formation of A&bgr; amyloid
    2.
    发明授权
    Vitro system for determining formation of A&bgr; amyloid 失效
    用于确定Aβ淀粉样蛋白形成的体外系统

    公开(公告)号：US06365414B1

    公开(公告)日：2002-04-02

    申请号：US08294819

    申请日：1994-08-26

    申请人： Rudolph E. Tanzi ,  Ashley I. Bush

    发明人： Rudolph E. Tanzi ,  Ashley I. Bush

    IPC分类号： G01N2175

    CPC分类号： G01N33/6896 ,  G01N2333/4709 ,  G01N2800/2821 ,  Y10S436/811 ,  Y10T436/25375

    摘要： The invention relates to rapid methods for determining formation of A&bgr; amyloid and screening compounds which inhibit formation of A&bgr; amyloid in vitro, as well as kits for carrying out the present methods. Such an agent used in vivo may prevent, ameliorate or reverse the symptoms of Alzheimer's disease and A&bgr; amyloidotic disorders related to Alzheimer's disease, Down's syndrome, and Guamanian amyotrophic lateral sclerosis/Parkinson's dementia complex. The process described in this invention involves the rapid induction of A&bgr; amyloid by a heavy metal cation capable of binding to a polypeptide comprising at least amino acids 6 to 28 of A&bgr;, such as zinc to form amyloid and determination of formation of tinctorial A&bgr; amyloid. Moreover, a method of determining effectiveness of a candidate anti-amyloidotic agent for prevention or treatment of A&bgr; amyloidosis is described which uses cell cultures which express at least a human A&bgr; peptide.

    摘要翻译： 本发明涉及用于确定Aβ淀粉样蛋白的形成和抑制体外抑制Aβ淀粉样蛋白形成的化合物的快速方法，以及用于实施本发明方法的试剂盒。 在体内使用的这种药剂可以预防，改善或逆转与阿尔茨海默病，唐氏综合征和瓜马尼亚肌萎缩性侧索硬化/帕金森氏综合征相关的阿尔茨海默氏病和阿贝氏淀粉样变性疾病的症状。 本发明描述的方法涉及通过能够与至少包含至少6至28个氨基酸的氨基酸（例如锌）的多肽结合的重金属阳离子快速诱导Aβ淀粉样蛋白，以形成淀粉样蛋白并测定着色的淀粉样蛋白的形成。 此外，描述了确定用于预防或治疗Aβ淀粉样变性的候选抗淀粉样变性剂的有效性的方法，其使用至少表达人Aβ肽的细胞培养物。

    Diagnostic assay for Alzheimer's disease: assessment of Aβ abnormalities
    3.
    发明授权
    Diagnostic assay for Alzheimer's disease: assessment of Aβ abnormalities 失效
    阿尔茨海默病的诊断测定：Abeta异常的评估

    公开(公告)号：US06890727B2

    公开(公告)日：2005-05-10

    申请号：US09425956

    申请日：1999-10-25

    申请人： Rudolph E. Tanzi ,  Ashley I. Bush ,  Robert D. Moir

    发明人： Rudolph E. Tanzi ,  Ashley I. Bush ,  Robert D. Moir

    IPC分类号： G01N33/553 ,  G01N33/53

    CPC分类号： G01N33/553 ,  G01N2800/2821 ,  Y10S435/975

    摘要： The disclosed invention relates to assays for detecting and quantifying Aβ peptide, using solid supports that are coated with heavy metal cations, such as zinc (II) or copper (II) form of a nitriloacetic acid. Further, diagnostic kits are described which are used to carry out the assays of the present invention. An improvement in an assay for detection of Aβ peptide is suggested which comprises forming a heavy metal cation/solid support complex. The preferred heavy metal cations for this improvement are zinc (II) or copper (II) form of a nitriloacetic acid. Finally, methods and kits for bulk purification of Aβ peptides from biological fluids are taught.

    摘要翻译： 所公开的发明涉及使用用重金属阳离子如锌（II）或铜（II）形式的次氮基乙酸涂覆的固体支持物来检测和定量Aβ肽的测定法。 此外，描述了用于进行本发明的测定的诊断试剂盒。 提出了用于检测Abeta肽的测定的改进，其包括形成重金属阳离子/固体支持物复合物。 用于此改进的优选重金属阳离子是次锌乙酸锌（II）或铜（II）形式。 最后，教导了从生物液体中大量净化Abeta肽的方法和试剂盒。

    Diagnostic assay for Alzheimer's disease: assessment of A.beta. abnormalities
    6.
    发明授权
    Diagnostic assay for Alzheimer's disease: assessment of A.beta. abnormalities 失效
    阿尔茨海默病的诊断测定：Aβ异常的评估

    公开(公告)号：US5972634A

    公开(公告)日：1999-10-26

    申请号：US817423

    申请日：1997-08-04

    申请人： Rudolph E. Tanzi ,  Ashley I. Bush ,  Robert D. Moir

    发明人： Rudolph E. Tanzi ,  Ashley I. Bush ,  Robert D. Moir

    IPC分类号： G01N33/553 ,  G01N33/53

    CPC分类号： G01N33/553 ,  G01N2800/2821 ,  Y10S435/975

    摘要： The disclosed invention relates to assays for detecting and quantifying A.beta. peptide, using solid supports that are coated with heavy metal cations, such as zinc (II) or copper (II) form of a nitriloacetic acid. Further, diagnostic kits are described which are used to carry out the assays of the present invention. An improvement in an assay for detection of A.beta. peptide is suggested which comprises forming a heavy metal cation/solid support complex. The preferred heavy metal cations for this improvement are zinc (II) or copper (II) form of a nitriloacetic acid. Finally, methods and kits for bulk purification of A.beta. peptides from biological fluids are taught.

    摘要翻译： PCT No.PCT / US94 / 11895 Sec。 371日期：1997年8月4日 102（e）日期1997年8月4日PCT 1994年10月19日PCT PCT。 第WO96 / 12544号公报 日期1996年5月2日所公开的发明涉及用重金属阳离子如锌（II）或铜（II）形式的次氮基乙酸涂覆的固体支持物来检测和定量Aβ肽的测定法。 此外，描述了用于进行本发明的测定的诊断试剂盒。 提出了用于检测Aβ肽的测定的改进，其包括形成重金属阳离子/固体支持物复合物。 用于此改进的优选重金属阳离子是次锌乙酸锌（II）或铜（II）形式。 最后，教导了从生物液体中大量纯化Aβ肽的方法和试剂盒。

    Alpha-2-macroglobulin diagnostic test
    8.
    发明授权
    Alpha-2-macroglobulin diagnostic test 失效
    α-2-巨球蛋白诊断试验

    公开(公告)号：US06342350B1

    公开(公告)日：2002-01-29

    申请号：US09148503

    申请日：1998-09-04

    申请人： Rudolph E. Tanzi ,  Bradley T. Hyman ,  George W. Rebeck ,  Deborah L. Blacker

    发明人： Rudolph E. Tanzi ,  Bradley T. Hyman ,  George W. Rebeck ,  Deborah L. Blacker

    IPC分类号： C12Q168

    CPC分类号： C07K14/8107 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/172 ,  G01N33/6896 ,  G01N2800/2821

    摘要： The disclosed invention relates to a diagnostic method for Alzheimer's disease based on genotyping the Alpha-2-Macroglobulin locus. A statistically significant correlation was found between inheritance of particular alleles of the Alpha-2-Macroglobulin gene and the occurrence of Alzheimer's disease. The diagnostic method involves the isolation of nucleic acid from an individual and subsequent genotyping by means such as sequencing or restriction fragment length polymorphism analysis. The invention also provides a means of genotype analysis through protein isotyping Alpha-2-Macroglobulin variant proteins. Finally, kits for nucleic acid analysis or protein analysis are taught.

    摘要翻译： 所公开的发明涉及基于α-2-巨球蛋白基因座的基因分型的阿尔茨海默病的诊断方法。 发现α-2-巨球蛋白基因特异性等位基因遗传与阿尔茨海默病发生之间有统计学意义的相关性。 诊断方法包括通过诸如测序或限制性片段长度多态性分析的方法从个体和随后的基因分型中分离核酸。 本发明还提供通过蛋白质同种型分析α-2-巨球蛋白变体蛋白的基因型分析方法。 最后，教导了用于核酸分析或蛋白质分析的试剂盒。