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公开(公告)号:US20090263900A1
公开(公告)日:2009-10-22
申请号:US12386059
申请日:2009-04-13
CPC分类号: C12N15/907 , C12N15/63 , C12N15/8213
摘要: Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
摘要翻译: 本文公开了包含侧翼于一个或多个感兴趣序列的50-750个碱基对(例如,50-100个碱基对)的同源臂的线性供体分子。 包含这些分子的供体分子和/或组合物可用于将外源序列靶向整合到细胞基因组中的特定感兴趣区域的方法中。
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公开(公告)号:US09045763B2
公开(公告)日:2015-06-02
申请号:US13134766
申请日:2011-06-16
CPC分类号: C12N15/907 , C12N15/63 , C12N15/8213
摘要: Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for integration of an exogenous sequence into a specified region of interest in the genome of a cell.
摘要翻译: 本文公开了包含侧翼于一个或多个感兴趣序列的50-750个碱基对(例如,50-100个碱基对)的同源臂的线性供体分子。 包含这些分子的供体分子和/或组合物可用于将外源序列整合到细胞基因组中的特定感兴趣区域的方法中。
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公开(公告)号:US20110281361A1
公开(公告)日:2011-11-17
申请号:US13134766
申请日:2011-06-16
IPC分类号: C12N15/63
CPC分类号: C12N15/907 , C12N15/63 , C12N15/8213
摘要: Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for integration of an exogenous sequence into a specified region of interest in the genome of a cell.
摘要翻译: 本文公开了包含侧翼于一个或多个感兴趣序列的50-750个碱基对(例如,50-100个碱基对)的同源臂的线性供体分子。 包含这些分子的供体分子和/或组合物可用于将外源序列整合到细胞基因组中的特定感兴趣区域的方法中。
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公开(公告)号:US08822221B2
公开(公告)日:2014-09-02
申请号:US13341228
申请日:2011-12-30
CPC分类号: C12N15/907 , C07K14/435 , C07K14/43595 , C07K2319/81 , C12N9/22 , C12N15/1082 , C12N15/113 , C12N2800/10 , C12N2800/30 , C12Y301/21004
摘要: Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest.
摘要翻译: 本文公开了用于将外源序列靶向整合到人PPP1R12C基因座中的方法和组合物,例如用于表达感兴趣的多肽。
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公开(公告)号:US20080299580A1
公开(公告)日:2008-12-04
申请号:US12150103
申请日:2008-04-24
CPC分类号: C12N15/907 , C07K14/435 , C07K14/43595 , C07K2319/81 , C12N9/22 , C12N15/1082 , C12N15/113 , C12N2800/10 , C12N2800/30 , C12Y301/21004
摘要: Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest.
摘要翻译: 本文公开了用于将外源序列靶向整合到人PPP1R12C基因座中的方法和组合物,例如用于表达感兴趣的多肽。
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公开(公告)号:US20120142055A1
公开(公告)日:2012-06-07
申请号:US13341228
申请日:2011-12-30
CPC分类号: C12N15/907 , C07K14/435 , C07K14/43595 , C07K2319/81 , C12N9/22 , C12N15/1082 , C12N15/113 , C12N2800/10 , C12N2800/30 , C12Y301/21004
摘要: Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest.
摘要翻译: 本文公开了用于将外源序列靶向整合到人PPP1R12C基因座中的方法和组合物,例如用于表达感兴趣的多肽。
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公开(公告)号:US08110379B2
公开(公告)日:2012-02-07
申请号:US12150103
申请日:2008-04-24
CPC分类号: C12N15/907 , C07K14/435 , C07K14/43595 , C07K2319/81 , C12N9/22 , C12N15/1082 , C12N15/113 , C12N2800/10 , C12N2800/30 , C12Y301/21004
摘要: Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest.
摘要翻译: 本文公开了用于将外源序列靶向整合到人PPP1R12C基因座中的方法和组合物,例如用于表达感兴趣的多肽。
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公开(公告)号:US08945868B2
公开(公告)日:2015-02-03
申请号:US13188417
申请日:2011-07-21
申请人: Trevor Collingwood , Laurence J. N. Cooper , Philip D. Gregory , Michael C. Holmes , Jeffrey C. Miller , Edward J. Rebar , Andreas Reik , Fyodor Urnov
发明人: Trevor Collingwood , Laurence J. N. Cooper , Philip D. Gregory , Michael C. Holmes , Jeffrey C. Miller , Edward J. Rebar , Andreas Reik , Fyodor Urnov
CPC分类号: C07K14/70539 , A61K35/17 , C07K2319/81 , C12N15/907 , C12N2800/80
摘要: Disclosed herein are methods and compositions for modulating the expression of a HLA locus or for selectively deleting or manipulating a HLA locus or HLA regulator.
摘要翻译: 本文公开了用于调节HLA基因座表达或选择性删除或操纵HLA基因座或HLA调节剂的方法和组合物。
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公开(公告)号:US08563314B2
公开(公告)日:2013-10-22
申请号:US12927557
申请日:2010-11-17
申请人: Philip D. Gregory , Michael C. Holmes , Matthew C. Mendel , Xiangdong Meng , David Paschon , Andreas Reik , Fyodor Urnov
发明人: Philip D. Gregory , Michael C. Holmes , Matthew C. Mendel , Xiangdong Meng , David Paschon , Andreas Reik , Fyodor Urnov
CPC分类号: A61K38/1709 , A61K35/17 , A61P31/12 , A61P35/00 , C07K2319/71 , C12N9/22
摘要: Disclosed herein are methods and compositions for modulating expression of a PD1 gene.
摘要翻译: 本文公开了调节PD1基因表达的方法和组合物。
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公开(公告)号:US20110027235A1
公开(公告)日:2011-02-03
申请号:US12798749
申请日:2010-04-08
申请人: Philip D. Gregory , Dirk Hockemeyer , Michael C. Holmes , Rudolf Jaenisch , Frank Soldner , Fyodor Urnov , Shuyuan Yao
发明人: Philip D. Gregory , Dirk Hockemeyer , Michael C. Holmes , Rudolf Jaenisch , Frank Soldner , Fyodor Urnov , Shuyuan Yao
CPC分类号: C12N15/86 , C12N9/22 , C12N2710/10343 , C12N2740/15043 , C12N2800/30 , C12N2830/008
摘要: Disclosed herein are methods and compositions for targeted integration of sequences of interest such as lineage-specific or cell fate reporter constructs or protein encoding sequences into stem cells.
摘要翻译: 本文公开了用于靶向整合感兴趣序列的方法和组合物,例如谱系特异性或细胞命运报道构建体或蛋白质编码序列到干细胞中。
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