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公开(公告)号：US20120100612A1
公开(公告)日：2012-04-26
申请号：US13379416
申请日：2010-06-22
申请人： Ryosuke Takahashi , Akiko Hisada , Hiroshi Sonoda , Taku Saito
发明人： Ryosuke Takahashi , Akiko Hisada , Hiroshi Sonoda , Taku Saito
IPC分类号： C12N5/0735 , C12N5/071 , C12M3/00
CPC分类号： C12N5/0062 , C12M21/08 , C12M23/12 , C12M25/06 , C12N2535/10
摘要： Disclosed is a culture sheet which enables technology in which three-dimensional tissues with uniform diameter are formed without applying chemicals to the surface of a culture substrate. A plurality of holes are formed on the culture sheet of the substrate, and nanopillars capable of controlling the adhesiveness or migration of a cell are formed on a culture surface that serves as the bottom surface of each of the holes. The culture surface of each of the holes having a structure in which a partition wall is provided, wherein, by forming the internal nanopillars in the vicinity of the center of each of the holes, the interaction of the disseminated cells can be limited to uniform the size of the three-dimensional structures of the cells to be formed.
摘要翻译： 公开了一种能够形成均匀直径的三维组织而不向培养基材表面施加化学物质的培养片。 在基板的培养片上形成多个孔，在作为各孔的底面的培养面上形成能够控制细胞的粘附性或迁移的纳米柱。 每个孔的培养面具有其中设置隔壁的结构，其中，通过在每个孔的中心附近形成内部纳米柱，可以将扩散细胞的相互作用限制为均匀的 要形成的细胞的三维结构的尺寸。
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公开(公告)号：US20110269232A1
公开(公告)日：2011-11-03
申请号：US13143569
申请日：2009-01-08
申请人： Ryosuke Takahashi , Akiko Hisada , Hiroshi Sonoda
发明人： Ryosuke Takahashi , Akiko Hisada , Hiroshi Sonoda
CPC分类号： C12N5/0671 , C12N2500/25 , C12N2501/39 , C12N2533/54
摘要： Provided are a technique for easily forming a spheroid by three-dimensionally culturing hepatocytes, and a technique for forming a spheroid having a higher expression level of a transporter MRP2 playing a role of biliary excretion than that of a conventional method. In order to solve the above-described problems, the present inventors have found out a condition under which hepatocytes easily form the spheroid on a nanopillar sheet. More specifically, this is related to a concentration of Type I collagen coated onto the NP sheet. Also, they have found out a condition under which an expression level of a gene related to the excretion of the formed spheroid is improved. More specifically, after the spheroid is previously formed, a biological matrix is overlayered thereon.
摘要翻译： 提供通过三维培养肝细胞容易地形成球状体的技术，以及形成具有比常规方法具有胆汁排泄作用的转运蛋白MRP2更高表达水平的球状体的技术。 为了解决上述问题，本发明人发现肝细胞容易在纳米柱片材上形成球状体的条件。 更具体地说，这涉及涂覆在NP片上的I型胶原蛋白的浓度。 此外，他们发现了与形成的球体的排泄相关的基因的表达水平提高的条件。 更具体地说，在预先形成球体之后，将生物体叠加在其上。
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公开(公告)号：US20130323839A1
公开(公告)日：2013-12-05
申请号：US13996074
申请日：2010-12-22
申请人： Ryosuke Takahashi , Akiko Hisada , Hiroshi Sonoda , Taku Saito , Naoshi Itabashi , Jiro Yamamoto
发明人： Ryosuke Takahashi , Akiko Hisada , Hiroshi Sonoda , Taku Saito , Naoshi Itabashi , Jiro Yamamoto
IPC分类号： G01N33/50
CPC分类号： G01N33/5008 , C12M25/00 , C12M25/06
摘要： Provided is a culture sheet which enables a technique for forming a three-dimensional tissue having uniform diameter without applying any chemical on the surface of a culture substrate. On the culture sheet (150) of the culture substrate, a plurality of holes (152) are formed and nanopillars (153), which are capable of controlling the adhesiveness and migration ability of cells, are formed on the bottom surface of each hole (152), said bottom face serving as a culture surface. The culture surface of each hole (151) is provided with a partition wall (152) and the internal nanopillars (153) are formed in the vicinity of the center of the hole (151). Owing to this configuration, the interaction among the disseminated cells can be restricted so that uniformly sized three-dimensional structures of the cells can be formed.
摘要翻译： 提供一种培养片，其能够形成具有均匀直径的三维组织的技术，而不在培养基材的表面上施加任何化学物质。 在培养基板的培养板（150）上形成有多个孔（152），并且能够控制细胞的粘附性和迁移能力的纳米柱（153）形成在各孔的底面 152），所述底面用作培养表面。 每个孔（151）的培养表面设置有分隔壁（152），并且内孔纳米柱（153）形成在孔（151）的中心附近。 由于这种构造，可以限制扩散细胞之间的相互作用，从而可以形成均匀尺寸的细胞的三维结构。
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公开(公告)号：US09394514B2
公开(公告)日：2016-07-19
申请号：US13379416
申请日：2010-06-22
申请人： Ryosuke Takahashi , Akiko Hisada , Hiroshi Sonoda , Taku Saito
发明人： Ryosuke Takahashi , Akiko Hisada , Hiroshi Sonoda , Taku Saito
CPC分类号： C12N5/0062 , C12M21/08 , C12M23/12 , C12M25/06 , C12N2535/10
摘要： Disclosed is a culture sheet which enables technology in which three-dimensional tissues with uniform diameter are formed without applying chemicals to the surface of a culture substrate. A plurality of holes are formed on the culture sheet of the substrate, and nanopillars capable of controlling the adhesiveness or migration of a cell are formed on a culture surface that serves as the bottom surface of each of the holes. The culture surface of each of the holes having a structure in which a partition wall is provided, wherein, by forming the internal nanopillars in the vicinity of the center of each of the holes, the interaction of the disseminated cells can be limited to uniform the size of the three-dimensional structures of the cells to be formed.
摘要翻译： 公开了一种能够形成均匀直径的三维组织而不向培养基材表面施加化学物质的培养片。 在基板的培养片上形成多个孔，在作为各孔的底面的培养面上形成能够控制细胞的粘附性或迁移的纳米柱。 每个孔的培养面具有其中设置隔壁的结构，其中，通过在每个孔的中心附近形成内部纳米柱，可以将扩散细胞的相互作用限制为均匀的 要形成的细胞的三维结构的尺寸。
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5.发明申请公开(公告)号：US20140162351A1
公开(公告)日：2014-06-12
申请号：US14131767
申请日：2011-08-29
申请人： Jiro Yamamoto , Naoshi Itabashi , Taku Saito , Akiko Hisada , Ryosuke Takahashi , Hiroshi Sonoda
发明人： Jiro Yamamoto , Naoshi Itabashi , Taku Saito , Akiko Hisada , Ryosuke Takahashi , Hiroshi Sonoda
CPC分类号： C12N5/0602 , B29C59/022 , C12M23/12 , C12M25/00 , C12N5/04
摘要： An operating efficiency of an observer is considerably restricted since it is not known at which position a culturing cell is disposed among a great number of pieces of holes of a culturing sheet. The culturing sheet is configured by a partitioning wall, a hole isolated by the partitioning wall, a local culturing region formed with plural local culturing region pillars a height of which is lower than that of the partitioning wall at a portion of a bottom face, and identification mark pillars formed at an identification mark region which differs from the culturing region at the bottom face of the hole. An identification mark is prevented from being unable to be optically recognized by adhering a spheroid to the identification mark region by making a diameter and a height of the identification mark pillar smaller than a diameter and a height of the local culturing pillar.
摘要翻译： 观察者的操作效率受到相当的限制，因为在培养板的大量孔中不设置培养单元的位置。 培养片由分隔壁，由分隔壁隔离的空穴构成，局部培养区形成有多个局部培养区柱，其高度低于底面部分的分隔壁， 识别标记柱形成在与孔底面的培养区域不同的识别标记区域。 通过使识别标记柱的直径和高度小于局部培养柱的直径和高度，通过将球体粘附到识别标记区域来防止识别标记不被光学识别。
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公开(公告)号：US08530237B2
公开(公告)日：2013-09-10
申请号：US13143569
申请日：2009-01-08
申请人： Ryosuke Takahashi , Akiko Hisada , Hiroshi Sonoda
发明人： Ryosuke Takahashi , Akiko Hisada , Hiroshi Sonoda
CPC分类号： C12N5/0671 , C12N2500/25 , C12N2501/39 , C12N2533/54
摘要： Provided are a technique for easily forming a spheroid by three-dimensionally culturing hepatocytes, and a technique for forming a spheroid having a higher expression level of a transporter MRP2 playing a role of biliary excretion than that of a conventional method. In order to solve the above-described problems, the present inventors have found out a condition under which hepatocytes easily form the spheroid on a nanopillar sheet. More specifically, this is related to a concentration of Type I collagen coated onto the NP sheet. Also, they have found out a condition under which an expression level of a gene related to the excretion of the formed spheroid is improved. More specifically, after the spheroid is previously formed, a biological matrix is overlayered thereon.
摘要翻译： 提供通过三维培养肝细胞容易地形成球状体的技术，以及形成具有比常规方法具有胆汁排泄作用的转运蛋白MRP2更高表达水平的球状体的技术。 为了解决上述问题，本发明人发现肝细胞容易在纳米柱片材上形成球状体的条件。 更具体地说，这涉及涂覆在NP片上的I型胶原蛋白的浓度。 此外，他们发现了与形成的球体的排泄相关的基因的表达水平提高的条件。 更具体地说，在预先形成球体之后，将生物体叠加在其上。
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公开(公告)号：US4726862A
公开(公告)日：1988-02-23
申请号：US33827
申请日：1987-04-03
申请人： Shigeru Goi , Taizo Sugihara , Hiroshi Sonoda
发明人： Shigeru Goi , Taizo Sugihara , Hiroshi Sonoda
摘要： In a process for producing tubular shaped fibrous articles of small diameter by heating and cooling a fibrous bundle containing at least 20 weight % of hot-melt-adhesive composite fibers, the improvements comprise using a shaping apparatus including an injecting chamber, an injecting hole formed in the wall of the chamber, a fibrous bundle outlet provided with a nozzle of a desired shape in cross-section, a cylindrical pipe for introducing the fibrous bundle, which has a cross-sectional area larger than that of the outlet, is located at a position opposite to the outlet and projects toward the outlet and terminates in the injecting chamber, and a core pipe which is open at its base on the outside of the injecting chamber, has its one end inserted through the cylindrical pipe and extending into the nozzle through the injecting chamber, and having a vent in its portion exposed within the injecting chamber, and passing the fibrous bundle through the cylindrical pipe to the outlet, while injecting a hot compressed gas through the injecting hole, thereby to heat and shape the fibrous bundle to and at its hot-melt-adhesive temperature.
摘要翻译： 在通过加热和冷却含有至少20重量％的热熔性复合纤维的纤维束的生产小直径的管状纤维制品的方法中，改进包括使用成型设备，其包括注射室，形成的注射孔 在室的壁中，具有横截面为所需形状的喷嘴的纤维束出口，具有大于出口的横截面面积的横截面积的用于引入纤维束的圆柱形管子位于 与出口相对的位置朝向出口突出并终止在注射室中，并且在其注射室外侧的基部处开放的芯管的一端通过圆柱形管插入并延伸到喷嘴中 通过注射室，并且在其部分暴露在注射室内的通气口，并使纤维束通过圆柱形管道到出口，而i 通过注射孔喷射热的压缩气体，从而将纤维束加热并成形为其热熔粘合温度。
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公开(公告)号：US4197156A
公开(公告)日：1980-04-08
申请号：US868362
申请日：1978-01-10
CPC分类号： B29C67/04 , B01D17/045 , B01D29/111 , B01D29/216 , B01D29/237 , B01D39/163 , B01D2201/182 , B01D2239/0216 , B01D2239/0407 , B01D2239/0478 , B01D2239/0613 , B01D2239/064 , B01D2239/065 , B01D2239/0668 , B01D2239/0681 , B01D2239/0695 , B01D2239/10
摘要： A method for producing a hollow-cylindrically shaped fibrous article stabilized by hot adhesion and an apparatus suitable to produce the same are provided. Said method comprises passing a web of gathered fiber layer carried on a conveyer belt through a heating zone, heating said web in such a way that a lower-melting component of composite fiber contained in the lower part of said web contacting the conveyer belt is not in the molten state and a lower-melting component contained in the upper part of said web is in the molten state, while separating said web from the conveyer belt, winding up said web on a take-up rod or tube in such a way that the upper surface thereof occupies the inner side of the winding, while heating the web further, cooling the wound up article and drawing out the take-up rod or tube from the shaped product.The production method of the present invention is simple and does not require complicated apparatus and the resultant product is useful for filtration.
摘要翻译： 提供一种用于制造通过热粘合而稳定的中空圆柱形纤维制品的方法和适于制造其的装置。 所述方法包括将承载在输送带上的聚集的纤维层的纤维网通过加热区，加热所述纤维网，使得包含在所述纤维网的下部的复合纤维的较低熔点成分与传送带接触不接近 在熔融状态下，并且包含在所述幅材的上部中的较低熔点成分处于熔融状态，同时将所述幅材与输送带分离，将所述幅材卷绕在卷取杆或管上，使得 其上表面占据绕组的内侧，同时进一步加热幅材，冷却卷绕物品并从成形产品中拉出卷取杆或管。 本发明的制造方法简单，不需要复杂的装置，所得产物可用于过滤。
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9.发明授权公开(公告)号：US06329529B1
公开(公告)日：2001-12-11
申请号：US09126718
申请日：1998-07-31
申请人： Hiroshi Sonoda , Kazunari Okada , Akira Takahashi , Kouki Fukumura , Hidetoshi Hayashi , Teruyuki Nagata , Yasuhiro Takano
发明人： Hiroshi Sonoda , Kazunari Okada , Akira Takahashi , Kouki Fukumura , Hidetoshi Hayashi , Teruyuki Nagata , Yasuhiro Takano
IPC分类号： C07D23368
CPC分类号： C07D233/68 , C07B39/00 , C07C17/16 , C07C17/18 , C07C51/60 , C07C205/12 , C07D233/02 , C07C22/08 , C07C19/01 , C07C21/18 , C07C22/04 , C07C19/075 , C07C23/10 , C07C19/08 , C07C25/18 , C07C25/13
摘要： Disclosed are a fluorinating agent represented by the general formula (1): wherein R1 to R4 are a substituted or unsubstituted, saturated or unsaturated alkyl group or a substituted or unsubstituted aryl group, and can be the same or different; R1 and R2 or R3 and R4 can bond to form a ring including a nitrogen atom or a nitrogen atom and other hetero atoms; or R1 and R3 can bond to form a ring including a nitrogen atom or a nitrogen atom and other hetero atoms, for example: a preparation process of the fluorinating agent and a process for preparing fluorine compounds by reacting various compounds with the fluorinating agent. The invention has also disclosed that the fluorinating agent is very effective for fluorinating oxygen containing functional compounds.
摘要翻译： 公开了由通式（1）表示的氟化剂：其中R 1至R 4是取代或未取代的饱和或不饱和的烷基或取代或未取代的芳基，并且可以相同或不同; R1和R2或R3和R4可键合形成包含氮原子或氮原子和其它杂原子的环; 或者R1和R3可以键合形成包含氮原子或氮原子和其它杂原子的环，例如：氟化剂的制备方法和通过使各种化合物与氟化剂反应制备氟化合物的方法。 本发明还公开了氟化剂对于含氟官能化合物的氟化是非常有效的。
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10.发明授权公开(公告)号：US06222046B1
公开(公告)日：2001-04-24
申请号：US09370213
申请日：1999-08-09
申请人： Hidetoshi Hayashi , Hiroshi Sonoda , Kenichi Goto , Kouki Fukumura , Junko Naruse , Teruyuki Nagata
发明人： Hidetoshi Hayashi , Hiroshi Sonoda , Kenichi Goto , Kouki Fukumura , Junko Naruse , Teruyuki Nagata
IPC分类号： C07D23902
CPC分类号： C07D233/28 , C07C67/08 , Y02P20/582 , C07C69/63
摘要： The invention has disclosed a trifluoroacetoxylation agent and a preparation process of the agent, which is safe and ease to handle, very useful in industry, and represented by the formula (1); wherein R1 to R4 are a substituted or unsubstituted, saturated or unsaturated alkyl group or a substituted or unsubstituted aryl group and can be the same or different, R1 and R2 or R3 and R4 can bond to form a ring containing a nitrogen atom or a nitrogen atom and other hetero atoms, and R1 and R3 can bond to form a ring containing a nitrogen atom or a nitrogen atom and other hetero atoms.
摘要翻译： 本发明公开了一种三氯乙酰氧基化剂及其制备方法，其安全性和易于处理，在工业上非常有用，并且由式（1）表示：其中R 1至R 4为取代或未取代的饱和或不饱和的 烷基或取代或未取代的芳基并且可以相同或不同，R 1和R 2或R 3和R 4可以键合形成含有氮原子或氮原子和其它杂原子的环，并且R 1和R 3可键合到 形成含有氮原子或氮原子和其它杂原子的环。
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