Nucleic acid probe and novel method of assaying nucleic acid using the same
    1.
    发明授权
    Nucleic acid probe and novel method of assaying nucleic acid using the same 失效
    核酸探针和使用其测定核酸的新方法

    公开(公告)号:US07273700B2

    公开(公告)日:2007-09-25

    申请号:US10399407

    申请日:2002-03-27

    IPC分类号: C12Q1/68 C07H21/02 C07H21/04

    摘要: A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.

    摘要翻译: 用于核酸测定的新型核酸探针包括用含有至少一对荧光染料的多种荧光染料标记以诱导FRET的单链核酸,该对荧光染料包括能够服用的荧光染料(供体染料) 作为能够作为受体染料的供体染料和荧光染料(受体染料),其中核酸探针具有这样的碱基序列并用荧光染料标记,使得受体染料的荧光强度降低 与靶核酸杂交。 一种新型核酸测定方法使用探针。 探针和方法可以使用简单的装置并行测定测定系统中的一种或多种类型的靶核酸。

    Novel nucleic acid probe and novel method of assaying nucleic acid using the same
    2.
    发明申请
    Novel nucleic acid probe and novel method of assaying nucleic acid using the same 失效
    新型核酸探针和使用其的新型核酸分析方法

    公开(公告)号:US20050048485A1

    公开(公告)日:2005-03-03

    申请号:US10399407

    申请日:2002-03-27

    IPC分类号: C12Q1/68 G01N33/58 C07H21/04

    摘要: A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.

    摘要翻译: 用于核酸测定的新型核酸探针包括用含有至少一对荧光染料的多种荧光染料标记以诱导FRET的单链核酸,该对荧光染料包括能够服用的荧光染料(供体染料) 作为能够用作受体染料的供体染料和荧光染料(受体染料),其中核酸探针具有这样的碱基序列并用荧光染料标记,使得受体染料的荧光强度降低 与靶核酸杂交。 一种新型核酸测定方法使用探针。 探针和方法可以使用简单的装置并行测定测定系统中的一种或多种类型的靶核酸。