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公开(公告)号：US10093957B2
公开(公告)日：2018-10-09
申请号：US15083610
申请日：2016-03-29
Applicant: S.D. SIGHT DIAGNOSTICS LTD.
Inventor： Joseph Joel Pollak , Arnon Houri Yafin , Seth J. Salpeter
Abstract: Provided is a method for imaging a blood sample and a kit and system for executing the method. The method includes introducing a cell suspension including red blood cells onto a base surface of a carrier having a vertical height (H) being greater than or equal to a vertical depth (h) of the cell suspension when on the base carrier, the cell suspension including a cell concentration (C) being determined by a defined function; allowing the cells in the cell suspension to settle on the base surface of the carrier to form a monolayer of cells thereon; and acquiring at least one microscope image of at least a portion of the monolayer of cells; wherein the at least one microscope image is obtained by a microscope set to Depth Of Field that is not more than 20% of the vertical height of the cell suspension settled on the base surface.
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公开(公告)号：US20150316477A1
公开(公告)日：2015-11-05
申请号：US14440864
申请日：2014-06-30
Applicant: S.D. SIGHT DIAGNOSTICS LTD.
Inventor： Joseph Joel Pollak , Arnon Houri Yafin , Seth J. Salpeter
CPC classification number: C12Q1/04 , G01N1/2813 , G01N1/38 , G01N15/1463 , G01N15/1475 , G01N21/6428 , G01N21/6486 , G01N33/49 , G01N2015/0065 , G01N2021/6439 , G02B21/361
Abstract: Provided is a method for imaging a blood sample and a kit and system for executing the method. The method includes introducing a cell suspension including red blood cells onto a base surface of a carrier having a vertical height (H) being greater than or equal to a vertical depth (h) of the cell suspension when on the base carrier, the cell suspension including a cell concentration (C) being determined by a defined function; allowing the cells in the cell suspension to settle on the base surface of the carrier to form a monolayer of cells thereon; and acquiring at least one microscope image of at least a portion of the monolayer of cells; wherein the at least one microscope image is obtained by a microscope set to Depth Of Field that is not more than 20% of the vertical height of the cell suspension settled on the base surface.
Abstract translation: 提供了一种用于对血液样本进行成像的方法以及用于执行该方法的套件和系统。 该方法包括在载体的基底表面上引入包含红细胞的细胞悬浮液，所述载体的垂直高度（H）大于或等于细胞悬液的垂直深度（h） 包括由定义的功能确定的细胞浓度（C）; 允许细胞悬浮液中的细胞沉淀在载体的基底表面上以在其上形成单层细胞; 以及获取细胞单层的至少一部分的至少一个显微镜图像; 其中所述至少一个显微镜图像是通过设置在深度不到所述细胞悬液沉降在所述基面上的垂直高度的20％的显微镜得到的。
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公开(公告)号：US11434515B2
公开(公告)日：2022-09-06
申请号：US16106085
申请日：2018-08-21
Applicant: S.D. SIGHT DIAGNOSTICS LTD.
Inventor： Joseph Joel Pollak , Arnon Houri Yafin , Seth J. Salpeter
Abstract: Apparatus and methods are described including introducing a cell suspension comprising red blood cells into a carrier that is a closed cavity that includes a base surface, via an inlet defined by the carrier. The cells in the cell suspension are allowed to settle on the base surface of the carrier to form a monolayer of cells on the base surface of the carrier. At least one microscope image of at least a portion of the monolayer of cells is acquired. Other applications are also described.
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公开(公告)号：US09329129B2
公开(公告)日：2016-05-03
申请号：US14440864
申请日：2014-06-30
Applicant: S.D. SIGHT DIAGNOSTICS LTD.
Inventor： Joseph Joel Pollak , Arnon Houri Yafin , Seth J. Salpeter
CPC classification number: C12Q1/04 , G01N1/2813 , G01N1/38 , G01N15/1463 , G01N15/1475 , G01N21/6428 , G01N21/6486 , G01N33/49 , G01N2015/0065 , G01N2021/6439 , G02B21/361
Abstract: Provided is a method for imaging a blood sample and a kit and system for executing the method. The method includes introducing a cell suspension including red blood cells onto a base surface of a carrier having a vertical height (H) being greater than or equal to a vertical depth (h) of the cell suspension when on the base carrier, the cell suspension including a cell concentration (C) being determined by a defined function; allowing the cells in the cell suspension to settle on the base surface of the carrier to form a monolayer of cells thereon; and acquiring at least one microscope image of at least a portion of the monolayer of cells; wherein the at least one microscope image is obtained by a microscope set to Depth Of Field that is not more than 20% of the vertical height of the cell suspension settled on the base surface.
Abstract translation: 提供了一种用于对血液样本进行成像的方法以及用于执行该方法的套件和系统。 该方法包括在载体的基底表面上引入包含红细胞的细胞悬浮液，所述载体的垂直高度（H）大于或等于细胞悬液的垂直深度（h） 包括由定义的功能确定的细胞浓度（C）; 允许细胞悬浮液中的细胞沉淀在载体的基底表面上以在其上形成单层细胞; 以及获取细胞单层的至少一部分的至少一个显微镜图像; 其中所述至少一个显微镜图像是通过设置在深度不到所述细胞悬液沉降在所述基面上的垂直高度的20％的显微镜得到的。
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