公开(公告)号：US20200318150A1

公开(公告)日：2020-10-08

申请号：US16644444

申请日：2018-09-04

Applicant: SCARAB GENOMICS LLC

Inventor： Frederick R. BLATTNER ,  Robert E. NOVY ,  David A. FRISCH ,  Charles LANDRY ,  Hyunsic CHOI ,  Eric A. STEFFEN ,  John BRANDON

IPC: C12P21/06 ,  C12N9/18 ,  C12N1/20 ,  C07K14/34 ,  C07K14/285 ,  C07K14/21

Abstract: Provided herein are methods for the improved production of periplasmic-targeted recombinant proteins in E. coli host strains. Also provided are E. coli host strains with improved capacity for producing recombinant proteins.

公开(公告)号：US20230287472A1

公开(公告)日：2023-09-14

申请号：US18299479

申请日：2023-04-12

Applicant: SCARAB GENOMICS LLC

Inventor： Frederick R. BLATTNER ,  Robert E. NOVY ,  David A. FRISCH ,  Charles LANDRY ,  Hyunsic CHOI ,  Eric A. STEFFEN ,  John BRANDON

IPC: C12P21/06 ,  C07K14/21 ,  C07K14/285 ,  C07K14/34 ,  C12N1/20 ,  C12N9/18

CPC classification number: C12P21/06 ,  C07K14/21 ,  C07K14/285 ,  C07K14/34 ,  C12N1/20 ,  C12N9/18 ,  C07K2319/02

Abstract: Provided herein are E. coli host strains with improved capacity for producing recombinant proteins.

公开(公告)号：US20160298126A1

公开(公告)日：2016-10-13

申请号：US15036376

申请日：2014-11-13

Applicant: SCARAB GENOMICS, LLC

Inventor： Frederick R. BLATTNER ,  David A. FRISCH ,  Robert E. NOVY ,  Terrance M. HENKER ,  Eric A. STEFFEN ,  Jose A. SANCHEZ ,  Christopher R. BLATTNER ,  Hyunsic CHOI ,  Gyorgy POSFAI

IPC: C12N15/70 ,  C12R1/19 ,  C12N9/10

CPC classification number: C12N15/70 ,  C12N9/1022 ,  C12N9/1048 ,  C12R1/19 ,  C12Y202/01006 ,  C12Y204/00

Abstract: E. coli bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.

Abstract translation: 提供包含遗传修饰以增强发酵能力和产生蛋白质和核酸的大肠杆菌。

公开(公告)号：US20180208933A1

公开(公告)日：2018-07-26

申请号：US15904297

申请日：2018-02-23

Applicant: SCARAB GENOMICS, LLC

Inventor： Frederick R. BLATTNER ,  David A. FRISCH ,  Robert E. NOVY ,  Terrance M. HENKER ,  Eric A. STEFFEN ,  Jose A. SANCHEZ ,  Christopher R. BLATTNER ,  Hyunsic CHOI ,  Gyorgy POSFAI

IPC: C12N15/70 ,  C12N9/10 ,  C12R1/19

CPC classification number: C12N15/70 ,  C12N9/1022 ,  C12N9/1048 ,  C12R1/19 ,  C12Y202/01006 ,  C12Y204/00

Abstract: E. coli bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.

公开(公告)号：US20170073379A1

公开(公告)日：2017-03-16

申请号：US15122891

申请日：2015-03-02

Applicant: SCARAB GENOMICS, LLC

Inventor： Cristopher R. BLATTNER ,  David FRISCH ,  Robert E. NOVY ,  Terrance M. HENKER ,  Eric A. STEFFEN ,  Frederick R. BLATTNER ,  Hyunsic CHOI ,  Gyorgy Posfai ,  Charles F. LANDRY

IPC: C07K14/34 ,  C12N1/20 ,  C12N1/08

CPC classification number: C07K14/34 ,  C12N1/08 ,  C12N1/20

Abstract: Rediced genome or native K12 strain E. coli bacteria comprising expression vectors encoding a recombinant CRM197 protein and their use in the production of CRM 197 is provided. The CRM 197 protein may be fused to a signal sequence that directs the expressed CRM197 protein to the periplasm of the E. coli host.

Abstract translation: 提供了包含编码重组CRM197蛋白的表达载体及其在生产CRM 197中的应用的经重修的基因组或天然K12菌株的大肠杆菌细菌。 CRM 197蛋白可以融合到将表达的CRM197蛋白质引导到大肠杆菌宿主的周质的信号序列上。