公开(公告)号：US11717825B2

公开(公告)日：2023-08-08

申请号：US16231251

申请日：2018-12-21

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Davide Cucchi ,  Lorenzo Bruno ,  Francesco Ferrara

IPC: B01L3/00 ,  F16K99/00 ,  B01L7/00 ,  B01L9/00

CPC classification number: B01L3/502738 ,  B01L7/5255 ,  B01L9/527 ,  F16K99/003 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0053 ,  B01L2200/027 ,  B01L2400/043 ,  B01L2400/0633 ,  B01L2400/0638

Abstract: The valve is formed in a valve body housing a first path portion, a second path portion, and an coupling zone between the first and second path portions. A shutter is arranged in the coupling zone and has a shutting portion of ferromagnetic material that is deformable under the action of an external magnetic field between an undeformed position, wherein the shutter closes the coupling zone, and a deformed position, wherein the shutter at least partially frees the coupling zone. The shutting portion of the shutter is formed by a rubber membrane incorporating particles, for example of ferrite particles.

公开(公告)号：US20150284707A1

公开(公告)日：2015-10-08

申请号：US14676626

申请日：2015-04-01

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Francesco Ferrara ,  Marco Angelo Bianchessi

IPC: C12N15/10 ,  B01L3/00 ,  G01N27/447

CPC classification number: C12N15/101 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0627 ,  C12Q1/6869 ,  C12Q2523/307 ,  C12Q2563/157 ,  C12Q2565/607 ,  G01N27/44791 ,  G01N33/48721

Abstract: An apparatus for nucleic acid sequencing includes a nanochannel and a conveying device, configured to move a nucleic acid strand through the nanochannel. The conveying device includes: a first electrode, a second electrode, and a third electrode, which are arranged along the nanochannel so as to be in contact with a fluid occupying the nanochannel, the second electrode being arranged between the first electrode and the third electrode; and a control unit configured to apply a first voltage, a second voltage, and a third voltage, respectively, to the first electrode, the second electrode and the third electrode, for controlling movement of the nucleic acid strand through the nanochannel.

Abstract translation: 用于核酸测序的装置包括纳米通道和输送装置，其被配置成使核酸链通过纳米通道移动。 输送装置包括：第一电极，第二电极和第三电极，其沿着纳米通道布置成与占据纳米通道的流体接触，第二电极布置在第一电极和第三电极之间 ; 以及控制单元，被配置为分别向第一电极，第二电极和第三电极施加第一电压，第二电压和第三电压，以控制核酸链通过纳米通道的运动。

公开(公告)号：US11278897B2

公开(公告)日：2022-03-22

申请号：US16224626

申请日：2018-12-18

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Davide Cucchi ,  Marco Angelo Bianchessi ,  Alessandro Cocci ,  Lillo Raia ,  Lorenzo Bruno ,  Nadia Serina ,  Marco Cereda ,  Danilo Pirola ,  Pietro Ferrari ,  Francesco Ferrara ,  Alessandro Paolo Bramanti

IPC: B01L3/00 ,  C12Q1/6806 ,  G01N1/34

Abstract: A sample treatment and molecule analysis cartridge is configured to be mounted in a treatment machine vertically. The cartridge has a sample inlet opening, a fluidic inlet, and a fluidic outlet. The cartridge houses an extraction chamber extending vertically from the sample inlet opening and connected to the fluidic inlet; a waste chamber extending vertically, alongside the extraction chamber; and a collector extending along the extraction chamber and the waste chamber and having a smaller height than the extraction chamber and the waste chamber. A fluidic circuit connects together the extraction chamber, the waste chamber, the collector, the fluidic inlet, and the fluidic outlet, and is configured to connect the fluidic outlet to vent openings of the extraction chamber, the waste chamber, and the collector, and to connect the bottom end of the extraction chamber to the fluidic inlet, the waste chamber, and the collector.

公开(公告)号：US20150284792A1

公开(公告)日：2015-10-08

申请号：US14676618

申请日：2015-04-01

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Marco Angelo Bianchessi ,  Francesco Ferrara

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B82Y30/00 ,  G01N27/4146

Abstract: An apparatus for nucleic acid sequencing includes: a base-detection device in a detection site, the base-detection device being configured to detect bases of a portion of a nucleic acid strand at the detection site; and a conveying device, configured to extend the nucleic acid strand and to cause the extended nucleic acid strand to slide through the detection site along a path. The base-detection device includes a plurality of field-effect nanowire detectors, arranged along the path and each including a respective nanowire and nucleic acid probes, which are defined by respective base sequences and are fixed to the respective nanowire.

Abstract translation: 用于核酸测序的装置包括：检测位点中的碱基检测装置，所述碱基检测装置被配置为检测所述检测部位的核酸链的一部分的碱基; 以及传送装置，其被配置为延伸所述核酸链并且使所述延伸的核酸链沿着路径滑动通过所述检测部位。 基底检测装置包括多个场效应纳米线检测器，沿着路径布置，每个包括相应的纳米线和核酸探针，它们由相应的碱基序列限定并固定在相应的纳米线上。

公开(公告)号：US20140352819A1

公开(公告)日：2014-12-04

申请号：US14289298

申请日：2014-05-28

Applicant: STMicroelectronics S.r.l.

Inventor： Marco Pugliese ,  Francesco Ferrara

IPC: F16K99/00

CPC classification number: F16K99/0053 ,  B01L3/502738 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655 ,  F16K99/0003 ,  F16K99/0015 ,  F16K99/0026 ,  F16K99/0046 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0092 ,  Y10T137/85978

Abstract: A microfluidic valve includes: a first structural layer and a second structural layer; a microfluidic circuit having a first microfluidic conduit and a second microfluidic conduit, which are defined in a superficial portion of the first structural layer, are adjacent, and are separated by a wall; a membrane set between the first structural layer and the second structural layer and delimiting the microfluidic circuit on one side; and a recess containing a gaseous fluid in the second structural layer. The membrane is movable in response to an actuation stimulus between a closed position, in which the first and second microfluidic conduits are fluidly decoupled, and an open position, in which the membrane is at least in part retracted into the recess and the first and second microfluidic conduits are fluidly coupled by means of a fluidic passage defined between the wall and the membrane.

Abstract translation: 微流体阀包括：第一结构层和第二结构层; 具有第一微流体管道和第二微流体管道的微流体回路限定在第一结构层的表面部分中，并且由壁分隔开; 在第一结构层和第二结构层之间设置膜并在一侧限定微流体电路; 以及在所述第二结构层中含有气态流体的凹部。 膜可以响应于在第一和第二微流体管道被流体分离的关闭位置和打开位置之间的致动刺激而移动，其中膜至少部分地缩回到凹部中，并且第一和第二 微流体导管通过限定在壁和膜之间的流体通道流体耦合。

公开(公告)号：US10197189B2

公开(公告)日：2019-02-05

申请号：US14289298

申请日：2014-05-28

Applicant: STMicroelectronics S.r.l.

Inventor： Marco Pugliese ,  Francesco Ferrara

IPC: B01L3/00 ,  F16K99/00

Abstract: A microfluidic valve includes: a first structural layer and a second structural layer; a microfluidic circuit having a first microfluidic conduit and a second microfluidic conduit, which are defined in a superficial portion of the first structural layer, are adjacent, and are separated by a wall; a membrane set between the first structural layer and the second structural layer and delimiting the microfluidic circuit on one side; and a recess containing a gaseous fluid in the second structural layer. The membrane is movable in response to an actuation stimulus between a closed position, in which the first and second microfluidic conduits are fluidly decoupled, and an open position, in which the membrane is at least in part retracted into the recess and the first and second microfluidic conduits are fluidly coupled by means of a fluidic passage defined between the wall and the membrane.

公开(公告)号：US10190161B2

公开(公告)日：2019-01-29

申请号：US14676618

申请日：2015-04-01

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Marco Angelo Bianchessi ,  Francesco Ferrara

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  B82Y30/00

Abstract: An apparatus for nucleic acid sequencing includes: a base-detection device in a detection site, the base-detection device being configured to detect bases of a portion of a nucleic acid strand at the detection site; and a conveying device, configured to extend the nucleic acid strand and to cause the extended nucleic acid strand to slide through the detection site along a path. The base-detection device includes a plurality of field-effect nanowire detectors, arranged along the path and each including a respective nanowire and nucleic acid probes, which are defined by respective base sequences and are fixed to the respective nanowire.

公开(公告)号：US09765326B2

公开(公告)日：2017-09-19

申请号：US14676626

申请日：2015-04-01

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Francesco Ferrara ,  Marco Angelo Bianchessi

IPC: G01N27/447 ,  C12N15/10 ,  B01L3/00 ,  G01N33/487 ,  C12Q1/68

CPC classification number: C12N15/101 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0627 ,  C12Q1/6869 ,  C12Q2523/307 ,  C12Q2563/157 ,  C12Q2565/607 ,  G01N27/44791 ,  G01N33/48721

Abstract: An apparatus for nucleic acid sequencing includes a nanochannel and a conveying device, configured to move a nucleic acid strand through the nanochannel. The conveying device includes: a first electrode, a second electrode, and a third electrode, which are arranged along the nanochannel so as to be in contact with a fluid occupying the nanochannel, the second electrode being arranged between the first electrode and the third electrode; and a control unit configured to apply a first voltage, a second voltage, and a third voltage, respectively, to the first electrode, the second electrode and the third electrode, for controlling movement of the nucleic acid strand through the nanochannel.