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    SYSTEMS AND METHODS FOR CONTINUOUS FLOW PCR SYSTEMS
    1.
    发明申请
    SYSTEMS AND METHODS FOR CONTINUOUS FLOW PCR SYSTEMS 审中-公开
    连续流动PCR系统的系统和方法

    公开(公告)号：US20160348190A1

    公开(公告)日：2016-12-01

    申请号：US15136679

    申请日：2016-04-22

    Applicant: STOKES BIO LIMITED

    Inventor： Mauro Aguanno ,  David Kinahan ,  Mark Korenke ,  Ryan Talbot ,  Mark Gaughran ,  Matthew Lough

    IPC: C12Q3/00 ,  B01L7/00 ,  G01N35/08 ,  C12Q1/68

    CPC classification number: C12Q3/00 ,  B01L7/5255 ,  B01L2300/0627 ,  C12Q1/686 ,  G01N35/08 ,  G01N2035/00366

    Abstract: A liquid handling system of a PCR system is instructed to obtain a matrix of samples and reagents for a PCR experiment. A fluid pumping system of the PCR system is instructed to maintain a continuous flow of a transport fluid through a plurality of micro-channels that allows the mixture of the samples and the reagents producing a plurality of mixed sample droplets. One or more post-bridge detection values are received from a post-bridge detection system of the PCR system for each mixed sample droplet to determine if the mixed sample droplet is mixed correctly. A thermocycler of the PCR system is instructed to maintain one or more temperatures for cycling the temperature of the plurality of mixed sample droplets. One or more endpoint detection values are received from an endpoint detection system of the PCR system for each mixed sample droplet to analyze the PCR experiment.

    Abstract translation: 指示PCR系统的液体处理系统获得用于PCR实验的样品和试剂的基质。 指示PCR系统的流体泵送系统保持运输流体通过多个微通道的连续流动，允许样品和试剂的混合物产生多个混合样品液滴。 从PCR系统的后桥检测系统接收每个混合样品液滴的一个或多个后桥检测值，以确定混合样品液滴是否被正确混合。 指示PCR系统的热循环仪保持用于循环多个混合样品液滴的温度的一个或多个温度。 从每个混合样品液滴的PCR系统的端点检测系统接收一个或多个端点检测值，以分析PCR实验。

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