公开(公告)号：US20050118584A1

公开(公告)日：2005-06-02

申请号：US10491719

申请日：2002-10-07

申请人： Sachio Nomura ,  Kazumi Kenmotsu ,  Makoto Nagano ,  Yukiko Sagehashi ,  Toru Egashira ,  Masatoshi Kondo

发明人： Sachio Nomura ,  Kazumi Kenmotsu ,  Makoto Nagano ,  Yukiko Sagehashi ,  Toru Egashira ,  Masatoshi Kondo

IPC分类号： B01L3/00 ,  C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6837 ,  B01L3/50853 ,  B01L2200/0689 ,  B01L2300/044 ,  B01L2300/0819 ,  B01L2300/0887 ,  C12Q2561/109

摘要： There are provided a) a detection board having numerous wells on a surface thereof, b) a detection set including the detection board and an element serving as well sealing means, and c) a method of using the detection set b), which includes injecting, into each of the wells of the detection board, an element for detecting a liquid phase reaction, sealing the element into the well with the well sealing means, and detecting the liquid phase reaction in the well. Thus, there is provided means for efficiently performing a liquid phase reaction on the board in a manner similar to that of an existing microarray technique.

摘要翻译： 提供了a）在其表面上具有许多孔的检测板，b）包括检测板和用作密封装置的元件的检测组，以及c）使用检测装置b）的方法，其包括注射 进入检测板的每个孔中，用于检测液相反应的元件，用井密封装置将元件密封到井中，并检测井中的液相反应。 因此，提供了以与现有的微阵列技术相似的方式在板上有效地进行液相反应的装置。

公开(公告)号：US20080020466A1

公开(公告)日：2008-01-24

申请号：US11660372

申请日：2004-08-17

申请人： Sachio Nomura ,  Masatoshi Kondo ,  Kazumi Kenmotsu ,  Makoto Nagano ,  Yukiko Sagehashi ,  Yoko Kimura ,  Toru Egashira

发明人： Sachio Nomura ,  Masatoshi Kondo ,  Kazumi Kenmotsu ,  Makoto Nagano ,  Yukiko Sagehashi ,  Yoko Kimura ,  Toru Egashira

IPC分类号： G01N33/00 ,  B01L11/00

CPC分类号： G01N33/54386 ,  C12Q1/6827 ,  C12Q2561/109 ,  C12Q2527/125 ,  C12Q2547/101

摘要： Provided is a detection method employing a detection instrument having a plurality of wells at a surface thereof, the method including depositing a detection object, preferably in coexistence with a specific holding substance, onto the inner walls of the wells of the detection instrument; bringing a reaction solution into contact with the inner walls of the wells of the detection instrument on which the detection object has been deposited, the reaction solution containing a substance reactive with the detection object; and detecting a signal generated through this contact, to thereby evaluate the detection object. In this method, a liquid phase reaction can be efficiently performed in the detection instrument for carrying out a biological reaction (e.g., a microarray) so that samples contained in the wells are not mixed with one another, and that air bubbles are prevented from being generated in the wells.

摘要翻译： 本发明提供一种使用具有在其表面具有多个孔的检测仪器的检测方法，该方法包括将检测对象优选地与特定保持物质共存于检测仪器的孔的内壁上; 使反应溶液与其上沉积有检测对象的检测仪器的孔的内壁接触，反应溶液含有与检测对象反应的物质; 并且检测通过该接触产生的信号，从而评估检测对象。 在该方法中，可以在用于进行生物反应的检测仪器（例如，微阵列）中有效地进行液相反应，使得包含在孔中的样品彼此不混合，并且防止气泡 在井中产生。