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1.发明申请公开(公告)号：US20140256045A1
公开(公告)日：2014-09-11
申请号：US14198124
申请日：2014-03-05
Applicant: Samsung Electronics Co., Ltd.
Inventor： Soon-chun CHUNG , Hyun-min Koo , Ji-eun Kim , Jae-chan Park , Joon-song Park , Ju-young Lee
CPC classification number: C12N15/80 , C12N15/81 , C12N15/815
Abstract: Provided is a method of simultaneous co-integration of multiple nucleic acid sequences into a microbial organism comprising culturing a microbial organism to be transformed until a growth phase is reached in which a random integration is facilitated; and transforming the microbial organism by mixing the cultured microbial organism with a sample to be co-integrated into the microbial organism with, wherein the sample comprises more than one nucleic acid sequence, and the products generated from the method.
Abstract translation: 提供了将多个核酸序列同时整合到微生物生物体中的方法，包括培养要转化的微生物，直到达到促进随机整合的生长期; 以及通过将培养的微生物与待共同整合到微生物中的样品混合培养微生物生物体，其中样品包含多于一种的核酸序列，以及由该方法生成的产物。
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2.发明授权Mutant microorganism having improved 1,4-BDO productivity and method of preparing 1,4-BDO using the mutant microorganism 有权具有改善的1,4-BDO生产力的突变体微生物和使用突变微生物制备1,4-BDO的方法公开(公告)号：US09416379B2
公开(公告)日：2016-08-16
申请号：US13952410
申请日：2013-07-26
Applicant: Samsung Electronics Co., Ltd.
Inventor： Kyu-sang Lee , Young-min Lee , Woo-yong Lee , Hyun-min Koo , Jin-woo Kim , Young-kyoung Park , Jin-hwan Park , Hwa-young Cho , Jae-chan Park
CPC classification number: C12P7/18 , C12N9/0006 , C12N15/52 , C12N15/77 , C12Y101/01037 , C12Y101/05004 , Y02P20/52
Abstract: A recombinantly modified Corynebacterium glutamicum microorganism with an improved 1,4-butanediol (1,4-BDO) productivity relative to an unmodified Corynebacterium glutamicum microorganism, wherein activity of an enzyme catalyzing a conversion reaction between malate and oxaloacetate is inactivated or reduced relative to an unmodified Corynebacterium glutamicum microorganism, as well as a method of making and using same.
Abstract translation: 相对于未修饰的谷氨酸棒杆菌微生物，具有改进的1,4-丁二醇（1,4-BDO）生产力的重组修饰的谷氨酸棒杆菌微生物，其中催化苹果酸和草酰乙酸之间的转化反应的酶的活性相对于 未经修饰的谷氨酸棒杆菌微生物，以及其制备和使用方法。
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3.发明授权Method of construction of recombinant organisms using multiple genes co-integration 有权使用多个基因共同整合的重组生物的构建方法公开(公告)号：US09404116B2
公开(公告)日：2016-08-02
申请号：US14198124
申请日：2014-03-05
Applicant: Samsung Electronics Co., Ltd.
Inventor： Soon-chun Chung , Hyun-min Koo , Ji-eun Kim , Jae-chan Park , Joon-song Park , Ju-young Lee
CPC classification number: C12N15/80 , C12N15/81 , C12N15/815
Abstract: Provided is a method of simultaneous co-integration of multiple nucleic acid sequences into a microbial organism comprising culturing a microbial organism to be transformed until a growth phase is reached in which a random integration is facilitated; and transforming the microbial organism by mixing the cultured microbial organism with a sample to be co-integrated into the microbial organism with, wherein the sample comprises more than one nucleic acid sequence, and the products generated from the method.
Abstract translation: 提供了将多个核酸序列同时整合到微生物生物体中的方法，包括培养要转化的微生物，直到达到促进随机整合的生长期; 以及通过将培养的微生物与待共同整合到微生物中的样品混合培养微生物生物体，其中样品包含多于一种的核酸序列，以及由该方法生成的产物。
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