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    Method for Whole Surrounding Surface Display of Target Proteins Using Bacterial Exoporium
    1.
    发明申请
    Method for Whole Surrounding Surface Display of Target Proteins Using Bacterial Exoporium 失效
    使用细菌外植体的靶蛋白整体表面显示方法

    公开(公告)号：US20070269838A1

    公开(公告)日：2007-11-22

    申请号：US10572224

    申请日：2003-12-30

    申请人： Sang Lee ,  Tae Park ,  Jong Park ,  Seok Lee ,  Jae-Gu Pan ,  Heung-Chae Jung ,  Soo-Keun Choi

    发明人： Sang Lee ,  Tae Park ,  Jong Park ,  Seok Lee ,  Jae-Gu Pan ,  Heung-Chae Jung ,  Soo-Keun Choi

    IPC分类号： C12P21/06 ,  C12N1/21 ,  C07K14/195 ,  C07K14/52

    CPC分类号： C40B40/02 ,  C12N15/1037

    摘要： The present invention relates to a method for expressing a target protein on an exosporium forming the outermost surface of bacterial spores. More particularly, the present invention relates to a method for expressing a target protein on the surface of cells and spores using an exosporium as a matrix for surface expression, and methods for the production of a protein array, the production of antibodies, the separation of a certain substance from a mixture, bioconversion, and the improvement of a target protein, which are characterized by using the cells or spores having the target protein that was expressed on the surface by the above expression method. The method for expressing the target protein on the surface of the spore outer membrane of the gene carriers according to the present invention has effects in that a variety of the target proteins can be expressed and the level of surface expression of the target protein is increased compared to the existing technology, and also the structural stability of the gene carriers having the target protein expressed on their surface, the viability of the host, and the rapidity of the screening method, are greatly increased.

    摘要翻译： 本发明涉及在形成细菌孢子的最外表面的外孢上表达靶蛋白的方法。 更具体地说，本发明涉及使用外鎓作为表面表达基质在细胞和孢子表面上表达靶蛋白的方法，以及用于产生蛋白质阵列，产生抗体的方法，分离 特定于使用具有通过上述表达方法在表面表达的靶蛋白的细胞或孢子的来自混合物的某种物质，生物转化和靶蛋白的改良。 根据本发明的用于在基因载体的孢子外膜的表面上表达靶蛋白的方法具有以下效果：可以表达多种靶蛋白，并且与目标蛋白的表面表达水平相比提高了靶蛋白的表达表达水平 现有技术，以及表面上具有靶蛋白的基因载体的结构稳定性，宿主的活力以及筛选方法的快速性都大大提高。

    Method for expression of proteins on spore surface
    2.
    发明授权
    Method for expression of proteins on spore surface 有权
    在孢子表面上表达蛋白质的方法

    公开(公告)号：US07582426B2

    公开(公告)日：2009-09-01

    申请号：US10433577

    申请日：2001-12-07

    申请人： June-Hyung Kim ,  Byung-Gee Kim ,  Soo-Keun Choi ,  Heung-Chae Jung ,  Jae-gu Pan

    发明人： June-Hyung Kim ,  Byung-Gee Kim ,  Soo-Keun Choi ,  Heung-Chae Jung ,  Jae-gu Pan

    IPC分类号： C12Q1/68 ,  C12N9/36 ,  C12N1/14

    CPC分类号： C40B40/02 ,  C12N15/1037 ,  C12N15/75

    摘要： The present invention relates to a method for display of proteinson spore surface and a method for improving protein with rapidity using the same, which comprises the steps of (i) preparing a vector for spore surface display comprising a gene construct containing a gene encoding spore coat protein and a gene encoding a protein of interest, wherein, when expressed, the gene construct expresses a fusion protein between the spore coat protein and the protein of interest, (ii) transforming a host cell with the vector for spore surface display; (iii) displaying the protein of interest on a surface of a spore of the host cell; and (iv) recovering the spore displaying on its surface the protein of interest.

    摘要翻译： 本发明涉及用于显示孢子表面蛋白质的方法和使用该蛋白质快速提高蛋白质的方法，其包括以下步骤：（i）制备用于孢子表面展示的载体，其包含含有编码孢子涂层的基因的基因构建体 蛋白质和编码目的蛋白质的基因，其中当表达时，所述基因构建体在所述孢子外壳蛋白和所述蛋白质之间表达融合蛋白，（ii）用所述载体转化宿主细胞用于孢子表面显示; （iii）在宿主细胞的孢子的表面上显示感兴趣的蛋白质; 和（iv）回收其表面上显示出感兴趣的蛋白质的孢子。

    Method for whole surrounding surface display of target proteins using bacterial exosporium
    3.
    发明授权
    Method for whole surrounding surface display of target proteins using bacterial exosporium 失效
    使用细菌外植体的靶蛋白的整个周围表面显示的方法

    公开(公告)号：US08030064B2

    公开(公告)日：2011-10-04

    申请号：US10572224

    申请日：2003-12-30

    申请人： Sang Yup Lee ,  Tae Jung Park ,  Jong Pil Park ,  Seok Jae Lee ,  Jae-Gu Pan ,  Heung-Chae Jung ,  Soo-Keun Choi

    发明人： Sang Yup Lee ,  Tae Jung Park ,  Jong Pil Park ,  Seok Jae Lee ,  Jae-Gu Pan ,  Heung-Chae Jung ,  Soo-Keun Choi

    IPC分类号： C12N15/00 ,  C12N1/21

    CPC分类号： C40B40/02 ,  C12N15/1037

    摘要： The present invention relates to a method for expressing a target protein on an exosporium forming the outermost surface of bacterial spores. More particularly, the present invention relates to a method for expressing a target protein on the surface of cells and spores using an exosporium as a matrix for surface expression, and methods for the production of a protein array, the production of antibodies, the separation of a certain substance from a mixture, bioconversion, and the improvement of a target protein, which are characterized by using the cells or spores having the target protein that was expressed on the surface by the above expression method. The method for expressing the target protein on the surface of the spore outer membrane of the gene carriers according to the present invention has effects in that a variety of the target proteins can be expressed and the level of surface expression of the target protein is increased compared to the existing technology, and also the structural stability of the gene carriers having the target protein expressed on their surface, the viability of the host, and the rapidity of the screening method, are greatly increased.

    摘要翻译： 本发明涉及在形成细菌孢子的最外表面的外孢上表达靶蛋白的方法。 更具体地说，本发明涉及使用外鎓作为表面表达基质在细胞和孢子表面上表达靶蛋白的方法，以及用于产生蛋白质阵列，产生抗体的方法，分离 特定于使用具有通过上述表达方法在表面表达的靶蛋白的细胞或孢子的来自混合物的某种物质，生物转化和靶蛋白的改良。 根据本发明的用于在基因载体的孢子外膜的表面上表达靶蛋白的方法具有以下效果：可以表达多种靶蛋白，并且与目标蛋白的表面表达水平相比提高了靶蛋白的表达表达水平 现有技术，以及表面上具有靶蛋白的基因载体的结构稳定性，宿主的活力以及筛选方法的快速性都大大提高。

    Method of protein synthesis
    4.
    发明授权
    Method of protein synthesis 有权
    蛋白质合成方法

    公开(公告)号：US07785832B2

    公开(公告)日：2010-08-31

    申请号：US11335592

    申请日：2006-01-20

    申请人： Jae-Gu Pan ,  Heung-Chae Jung ,  Sooan Shin

    发明人： Jae-Gu Pan ,  Heung-Chae Jung ,  Sooan Shin

    IPC分类号： C12P21/00 ,  C07H21/04

    CPC分类号： C12N15/70 ,  C12N9/78 ,  C12N9/93 ,  C12Y602/01001

    摘要： The present invention relates to a method of protein synthesis and, more particularly, to a method of effective protein synthesis by regulating the expression of a protein in a host cell, wherein said host cell is transformed with an expression vector comprising a promoter as well as a DNA fragment for a gene that encodes a desired protein, wherein said promoter has an inductive activity for transcription during the resting stage of cell growth and also the induction of said protein expression can be controlled by varying culturing conditions.

    摘要翻译： 本发明涉及一种蛋白质合成方法，更具体地说，涉及通过调节宿主细胞中蛋白质表达的有效蛋白质合成方法，其中所述宿主细胞用包含启动子的表达载体以及 用于编码所需蛋白质的基因的DNA片段，其中所述启动子在细胞生长的静息期期间具有转录的诱导活性，并且还可以通过改变培养条件来控制所述蛋白质表达的诱导。