公开(公告)号：US07595156B2

公开(公告)日：2009-09-29

申请号：US10819386

申请日：2004-04-06

申请人： Sang Yup Lee ,  Zixin Deng ,  Shi Chen ,  Ki Jun Jeong ,  Xiufen Zhou

发明人： Sang Yup Lee ,  Zixin Deng ,  Shi Chen ,  Ki Jun Jeong ,  Xiufen Zhou

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12P19/62 ,  C07H21/04 ,  C07K14/36 ,  C12N15/52 ,  C12P17/08

摘要： The present invention relates to the base sequence of whole genes involved in the biosynthesis of FR-008 polyketides derived from Streptomyces sp. FR-008. This base sequence comprises genes coding for ketosynthase (KS), acyl transferase (AT), acyl carrier protein (ACP), ketoreductase (KR), dehydratase (DH) and enoyl reductase (ER) domains, and genes coding for modifier enzymes, such as ABC transporter, cytochrome P450 monooxygenase, ferredoxin, thioesterase, sugar synthetic protein, FAD-dependent monooxygenase, 4-amino-4-deoxychorismate (ADC) synthase and ADC lyase. The gene base sequence according to the present invention can be used to increase the productivity of the existing FR-008 polyketides or produce new FR-008 polyketides, through modification of its parts.

摘要翻译： 本发明涉及参与由链霉菌属（Streptomyces sp。）生产的FR-008聚酮化合物的生物合成的全基因的碱基序列。 FR-008。 该碱基序列包括编码酮解酶（KS），酰基转移酶（AT），酰基载体蛋白（ACP），酮还原酶（KR），脱水酶（DH）和烯酰还原酶（ER）结构域的基因，以及编码修饰酶的基因， 作为ABC转运蛋白，细胞色素P450单加氧酶，铁氧还蛋白，硫酯酶，糖合成蛋白，FAD依赖性单加氧酶，4-氨基-4-脱氧激酶（ADC）合酶和ADC裂解酶。 根据本发明的基因碱基序列可用于通过改变其部分来提高现有FR-008聚酮化合物的生产率或生产新的FR-008聚酮化合物。

公开(公告)号：US20120322684A1

公开(公告)日：2012-12-20

申请号：US13546972

申请日：2012-07-11

申请人： Sang Yup Lee ,  Nae Choon Yoo ,  So Young Yoo ,  Hyun Cheol Chung ,  Ki Chang Keum ,  Won Min Yoo ,  Ki Jun Jeong

发明人： Sang Yup Lee ,  Nae Choon Yoo ,  So Young Yoo ,  Hyun Cheol Chung ,  Ki Chang Keum ,  Won Min Yoo ,  Ki Jun Jeong

IPC分类号： C07K14/535 ,  C12Q1/68 ,  C40B30/04 ,  C07H21/04

CPC分类号： C07K16/243 ,  C12Q1/6886 ,  C12Q2600/156

摘要： Disclosed are a method, a composition, a microarray, an antibody and a kit for diagnosis and prognosis of cancer, based on detection of deletion of the exon 3 region of G-CSF gene or levels of a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to the exon 3 region, wherein the deletion of the exon 3 region of the G-CSF gene is used as a cancer biomarker.

摘要翻译： 公开了基于检测到G-CSF基因的外显子3区的缺失或具有缺失的突变的G-CSF蛋白的水平的方法，组合物，微阵列，抗体和用于癌症诊断和预后的试剂盒 的对应于外显子3区域的氨基酸序列，其中G-CSF基因的外显子3区域的缺失被用作癌症生物标志物。

公开(公告)号：US20100286381A1

公开(公告)日：2010-11-11

申请号：US12830085

申请日：2010-07-02

申请人： Sang Yup LEE ,  Nae Choon Yoo ,  So Young Yoo ,  Hyun Cheol Chung ,  Ki Chang Keum ,  Won Min Yoo ,  Ki Jun Jeong

发明人： Sang Yup LEE ,  Nae Choon Yoo ,  So Young Yoo ,  Hyun Cheol Chung ,  Ki Chang Keum ,  Won Min Yoo ,  Ki Jun Jeong

IPC分类号： C07H21/02 ,  C07H21/04

CPC分类号： C07K16/243 ,  C12Q1/6886 ,  C12Q2600/156

摘要： Disclosed are a method, a composition, a microarray, an antibody and a kit for diagnosis and prognosis of cancer, based on detection of deletion of the exon 3 region of G-CSF gene or levels of a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to the exon 3 region, wherein the deletion of the exon 3 region of the G-CSF gene is used as a cancer biomarker.

摘要翻译： 公开了基于检测到G-CSF基因的外显子3区的缺失或具有缺失的突变的G-CSF蛋白的水平的方法，组合物，微阵列，抗体和用于癌症诊断和预后的试剂盒 的对应于外显子3区域的氨基酸序列，其中G-CSF基因的外显子3区域的缺失被用作癌症生物标志物。

公开(公告)号：US20140005365A1

公开(公告)日：2014-01-02

申请号：US13546975

申请日：2012-07-11

申请人： Sang Yup Lee ,  Nae Choon Yoo ,  So Young Yoo ,  Hyun Cheol Chung ,  Ki Chang Keum ,  Won Min Yoo ,  Ki Jun Jeong

发明人： Sang Yup Lee ,  Nae Choon Yoo ,  So Young Yoo ,  Hyun Cheol Chung ,  Ki Chang Keum ,  Won Min Yoo ,  Ki Jun Jeong

IPC分类号： C07K16/24

CPC分类号： C07K16/243 ,  C12Q1/6886 ,  C12Q2600/156

摘要： Disclosed are a method, a composition, a microarray, an antibody and a kit for diagnosis and prognosis of cancer, based on detection of deletion of the exon 3 region of G-CSF gene or levels of a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to the exon 3 region, wherein the deletion of the exon 3 region of the G-CSF gene is used as a cancer biomarker.

摘要翻译： 公开了基于检测到G-CSF基因的外显子3区的缺失或具有缺失的突变的G-CSF蛋白的水平的方法，组合物，微阵列，抗体和用于癌症诊断和预后的试剂盒 的对应于外显子3区域的氨基酸序列，其中G-CSF基因的外显子3区域的缺失被用作癌症生物标志物。

公开(公告)号：US08324363B2

公开(公告)日：2012-12-04

申请号：US11058976

申请日：2005-02-16

申请人： Sang Yup Lee ,  Nae Choon Yoo ,  So Young Yoo ,  Hyun Cheol Chung ,  Ki Chang Keum ,  Won Min Yoo ,  Ki Jun Jeong

发明人： Sang Yup Lee ,  Nae Choon Yoo ,  So Young Yoo ,  Hyun Cheol Chung ,  Ki Chang Keum ,  Won Min Yoo ,  Ki Jun Jeong

IPC分类号： C07H21/04 ,  C12Q1/68 ,  C07K14/535

CPC分类号： C07K16/243 ,  C12Q1/6886 ,  C12Q2600/156

摘要： Disclosed are a method, a composition, a microarray, an antibody and a kit for diagnosis and prognosis of cancer, based on detection of deletion of the exon 3 region of G-CSF gene or levels of a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to the exon 3 region, wherein the deletion of the exon 3 region of the G-CSF gene is used as a cancer biomarker.

摘要翻译： 公开了基于检测到G-CSF基因的外显子3区的缺失或具有缺失的突变的G-CSF蛋白的水平的方法，组合物，微阵列，抗体和用于癌症诊断和预后的试剂盒 的对应于外显子3区域的氨基酸序列，其中G-CSF基因的外显子3区域的缺失被用作癌症生物标志物。

公开(公告)号：US20050260736A1

公开(公告)日：2005-11-24

申请号：US11084717

申请日：2005-03-18

申请人： George Georgiou ,  Ki Jun Jeong ,  Barrett Harvey ,  Brent Iverson

发明人： George Georgiou ,  Ki Jun Jeong ,  Barrett Harvey ,  Brent Iverson

IPC分类号： C12N1/21 ,  C12N15/10 ,  C12N15/74 ,  C40B40/02

CPC分类号： C40B40/02 ,  C07K2319/04 ,  C12N15/1037 ,  C12N15/1086

摘要： The invention overcomes the deficiencies of the prior art by providing a rapid approach for isolating polypeptides capable of anchoring heterologous polypeptides to a bacterial inner membrane. In the technique, libraries of candidate anchor polypeptides are expressed as fusions with a heterologous polypeptide that is capable of being detected when bound to the inner membrane. In bacteria expressing a functional anchor sequence, the heterologous polypeptide becomes bound to outer face of the inner membrane. Bacteria with the functional anchor sequence can be identified by removing the outer membrane to remove non-anchored heterologous polypeptide followed by detection of anchored heterologous polypeptide. Such bacteria may be detected in numerous ways, including use of direct fluorescence or secondary antibodies that are fluorescently labeled, allowing use of efficient techniques such as fluorescence activated cell sorting (FACS).

摘要翻译： 本发明克服了现有技术的缺陷，提供了一种用于分离能够将异源多肽固定在细菌内膜上的多肽的快速方法。 在该技术中，候选锚多肽的文库表达为与异源多肽的融合，当与内膜结合时能够被检测。 在表达功能性锚定序列的细菌中，异源多肽与内膜的外表面结合。 具有功能性锚定序列的细菌可以通过除去外膜以除去非锚定的异源多肽，然后检测锚定的异源多肽来鉴定。 可以以许多方式检测这些细菌，包括使用荧光标记的直接荧光或二次抗体，允许使用有效技术，例如荧光激活细胞分选（FACS）。

公开(公告)号：US07611866B2

公开(公告)日：2009-11-03

申请号：US11084717

申请日：2005-03-18

申请人： George Georgiou ,  Ki Jun Jeong ,  Barrett R. Harvey ,  Brent L. Iverson

发明人： George Georgiou ,  Ki Jun Jeong ,  Barrett R. Harvey ,  Brent L. Iverson

IPC分类号： C12P21/06 ,  C12P21/04 ,  C12N15/09 ,  G01N33/53 ,  G01N33/567 ,  G01N33/569 ,  G01N33/554

CPC分类号： C40B40/02 ,  C07K2319/04 ,  C12N15/1037 ,  C12N15/1086

摘要： The invention overcomes the deficiencies of the prior art by providing a rapid approach for isolating polypeptides capable of anchoring heterologous polypeptides to a bacterial inner membrane. In the technique, libraries of candidate anchor polypeptides are expressed as fusions with a heterologous polypeptide that is capable of being detected when bound to the inner membrane. In bacteria expressing a functional anchor sequence, the heterologous polypeptide becomes bound to outer face of the inner membrane. Bacteria with the functional anchor sequence can be identified by removing the outer membrane to remove non-anchored heterologous polypeptide followed by detection of anchored heterologous polypeptide. Such bacteria may be detected in numerous ways, including use of direct fluorescence or secondary antibodies that are fluorescently labeled, allowing use of efficient techniques such as fluorescence activated cell sorting (FACS).

摘要翻译： 本发明克服了现有技术的缺陷，提供了一种用于分离能够将异源多肽固定在细菌内膜上的多肽的快速方法。 在该技术中，候选锚多肽的文库表达为与异源多肽的融合，当与内膜结合时能够被检测。 在表达功能性锚定序列的细菌中，异源多肽与内膜的外表面结合。 具有功能性锚定序列的细菌可以通过除去外膜以除去非锚定的异源多肽，然后检测锚定的异源多肽来鉴定。 可以以许多方式检测这些细菌，包括使用荧光标记的直接荧光或二次抗体，允许使用有效技术，例如荧光激活细胞分选（FACS）。