-
公开(公告)号：US20220348864A1
公开(公告)日：2022-11-03
申请号：US17836294
申请日：2022-06-09
发明人： Takako Chikenji , Mineko Fujimiya , Yuki Saito , Masako Nakano , Miho Otani , Yuka Mizue , Takashi Matsumura , Kozue Kamiya
IPC分类号： C12N5/00 , A61K35/28 , C12N5/0775 , C12Q1/6881 , G01N33/569
摘要： [Problem] To provide a cell preparation including mesenchymal stem cells (MSCs) having a high therapeutic effect. [Solution] A method for producing activated mesenchymal stem cells, including a step of culturing MSCs in a medium containing an activator that includes an extract from a mammalian fetal appendage as an active ingredient, using a cell culture carrier having a three-dimensional structure formed of a fiber is provided. A marker for a therapeutic effect of MSCs selected from the group consisting of p16ink4a, p14ARF, CDK4, CDK6, RB, and CD47, a method for determining a therapeutic effect using the marker, a method for determining suitability of MSCs to be treated with a treatment for enhancing a therapeutic effect of MSCs, a cell preparation including MSCs, and a method for producing the same are also provided.
-
公开(公告)号：US12090251B2
公开(公告)日：2024-09-17
申请号：US17049867
申请日：2019-04-25
发明人： Takako Chikenji , Mineko Fujimiya , Yuki Saito , Masako Nakano , Naoto Konari , Miho Otani
CPC分类号： A61L27/3834 , A61K35/28 , A61L27/3878 , A61L27/56 , A61P13/12 , A61P25/28 , C12M21/08 , C12N5/0663 , C12N5/0667 , A61L2300/64 , A61L2430/26 , A61L2430/32
摘要： A cell sheet for transplantation into a living body, containing MSCs having an average cell density of 3.0×104 cells/cm2 or less on the surface of the sheet is provided. A method for producing a cell sheet for transplantation into a living body, including: a step of seeding MSCs on a cell culture carrier having a three-dimensional structure formed of fibers at a cell number of 3.0×105 cells/cm2 or less; and a step of culturing the MSCs and thereby preparing a cell sheet containing the MSCs having an average cell density of 3.0×104 cells/cm2 or less is also provided.
-
搜索结果
2 条记录 (耗时 0.012 秒)
