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    CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS IN MICROWELLS
    1.
    发明申请
    CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS IN MICROWELLS 审中-公开
    人胚胎干细胞在微阵列中的克隆

    公开(公告)号：US20080220520A1

    公开(公告)日：2008-09-11

    申请号：US12032986

    申请日：2008-02-18

    申请人： Sean P. Palecek ,  Juan J. DePablo ,  Jeffrey C. Mohr ,  Lin Ji

    发明人： Sean P. Palecek ,  Juan J. DePablo ,  Jeffrey C. Mohr ,  Lin Ji

    IPC分类号： C12N5/06 ,  C12M1/00

    CPC分类号： A01N1/02 ,  A01N1/0221 ,  A01N1/0231

    摘要： The present invention relates to methods and structures for preparing stem cells for use in cryopreservation methods. Stem cell colonies are provided between first and second matrix portions and are exposed to a carbohydrate-containing cryoprotecting medium and a freezing medium. The methods of the invention yield cryopreserved cells that maintain cell viability and exhibit limited cell differentiation after freezing and thawing, facilitating storage, shipping and handling of embryonic stem cell stocks and lines for research and therapeutics.

    摘要翻译： 本发明涉及用于制备用于冷冻保存方法的干细胞的方法和结构。 提供干细胞集落在第一和第二基质部分之间，并暴露于含碳水化合物的低温保护培养基和冷冻培养基中。 本发明的方法产生维持细胞存活力的冷冻保存的细胞，并且在冷冻和融化后表现出有限的细胞分化，促进胚胎干细胞种群和用于研究和治疗的品系的储存，运输和处理。

    METHOD FOR CULTURING STEM CELLS
    3.
    发明申请
    METHOD FOR CULTURING STEM CELLS 有权
    培养干细胞的方法

    公开(公告)号：US20100197013A1

    公开(公告)日：2010-08-05

    申请号：US12615044

    申请日：2009-11-09

    申请人： Timothy J. Kamp ,  Jianhua Zhang ,  Jeffrey C. Mohr ,  Juan J. Depablo ,  Sean P. Palecek

    发明人： Timothy J. Kamp ,  Jianhua Zhang ,  Jeffrey C. Mohr ,  Juan J. Depablo ,  Sean P. Palecek

    IPC分类号： C12N5/071 ,  C12N5/02 ,  C12N5/00 ,  C40B40/14

    CPC分类号： C07K16/18 ,  C12N5/0603 ,  C12N2533/90

    摘要： A three-dimensional microwell system that supports long term pluripotent cell culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured pluripotent cells remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel®-coated, tissue culture-treated polystyrene dishes. Microwell-cultured pluripotent cells maintain pluripotency, differentiating to each of the three embryonic germ layers. Pluripotent cell aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain pluripotent cell growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct pluripotent cell differentiation by generating monodisperse EBs of a desired size and shape. Cardiomyocyte-rich EBs are obtained from pluripotent cells cultured in microwells of defined size and shape.

    摘要翻译： 描述了支持长期多能细胞培养和形成均质胚状体（EB）的三维微孔系统。 微培养的多能细胞在培养物中保持存活和未分化数周，并在传播至经Matrigel®涂覆的组织培养处理的聚苯乙烯培养皿时保持未分化的复制。 微孔培养的多能细胞保持多能性，与三个胚胎胚层分化。 可以将从微孔释放的多能细胞聚集体传代为未分化复制或分化为单分散EB。 在三维中约束多能细胞生长的能力有利地提供了更有效，可重复的未分化细胞的培养，高通量筛选，以及通过产生所需大小和形状的单分散EB来引导多能细胞分化的能力。 富含心肌细胞的EB是从在规定尺寸和形状的微孔中培养的多能细胞获得的。

    Method for culturing stem cells
    5.
    发明授权
    Method for culturing stem cells 有权
    培养干细胞的方法

    公开(公告)号：US08956867B2

    公开(公告)日：2015-02-17

    申请号：US12615044

    申请日：2009-11-09

    申请人： Timothy J. Kamp ,  Jianhua Zhang ,  Jeffrey C. Mohr ,  Juan J. Depablo ,  Sean P. Palecek

    发明人： Timothy J. Kamp ,  Jianhua Zhang ,  Jeffrey C. Mohr ,  Juan J. Depablo ,  Sean P. Palecek

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C07K16/18 ,  C12N5/073

    CPC分类号： C07K16/18 ,  C12N5/0603 ,  C12N2533/90

    摘要： A three-dimensional microwell system that supports long term pluripotent cell culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured pluripotent cells remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel®-coated, tissue culture-treated polystyrene dishes. Microwell-cultured pluripotent cells maintain pluripotency, differentiating to each of the three embryonic germ layers. Pluripotent cell aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain pluripotent cell growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct pluripotent cell differentiation by generating monodisperse EBs of a desired size and shape. Cardiomyocyte-rich EBs are obtained from pluripotent cells cultured in microwells of defined size and shape.

    摘要翻译： 描述了支持长期多能细胞培养和形成均质胚状体（EB）的三维微孔系统。 微培养的多能细胞在培养物中保持存活和未分化数周，并在传播至经Matrigel®涂覆的组织培养处理的聚苯乙烯培养皿时保持未分化的复制。 微孔培养的多能细胞保持多能性，与三个胚胎胚层分化。 可以将从微孔释放的多能细胞聚集体传代为未分化复制或分化为单分散EB。 在三维中约束多能细胞生长的能力有利地提供了更有效，可重复的未分化细胞的培养，高通量筛选，以及通过产生所需大小和形状的单分散EB来引导多能细胞分化的能力。 富含心肌细胞的EB是从在规定尺寸和形状的微孔中培养的多能细胞获得的。

    Method and System for Dynamically Creating and Exploring Graph Structures
    9.
    发明申请
    Method and System for Dynamically Creating and Exploring Graph Structures 审中-公开

    公开(公告)号：US20180130239A1

    公开(公告)日：2018-05-10

    申请号：US15866539

    申请日：2018-01-10

    申请人： Jeffrey C. Mohr ,  Michael R. Mohr

    发明人： Jeffrey C. Mohr ,  Michael R. Mohr

    IPC分类号： G06T11/20 ,  G06F17/30

    CPC分类号： G06T11/206 ,  G06F17/30958

    摘要： A system and method are presented that store graph data in a database as nodes, edges that link nodes, and detached profiles that contain properties that provide additional context for the nodes and edges. Computerized methods are described that provide for the dynamic creation of graph data based on the properties stored on the profiles related to each node, including the ability to predetermine which properties are used within a CSS-like language. Detached profiles allow a single profile, comprising a set of attribute-value pairs, to be associated with multiple database objects, or to survive the deletion of associated objects.

    METHOD AND SYSTEM FOR DYNAMICALLY CREATING AND EXPLORING GRAPH STRUCTURES
    10.
    发明申请
    METHOD AND SYSTEM FOR DYNAMICALLY CREATING AND EXPLORING GRAPH STRUCTURES 审中-公开
    用于动态创建和探索图形结构的方法和系统

    公开(公告)号：US20150339835A1

    公开(公告)日：2015-11-26

    申请号：US14717493

    申请日：2015-05-20

    申请人： Jeffrey C. Mohr ,  Michael R. Mohr

    发明人： Jeffrey C. Mohr ,  Michael R. Mohr

    IPC分类号： G06T11/20 ,  G06F17/30

    CPC分类号： G06T11/206 ,  G06F17/30958

    摘要： A system and method are presented that store graph data in a database as nodes, edges that link nodes, and profiles that contain properties that provide additional context for the nodes and edges. Computerized methods are described that provide for the dynamic creation of graph data based on the properties stored on the profiles related to each node, including the ability to predetermine which properties are used within a CSS-like language. Methods for visually focusing on a portion of a graph are also disclosed that allow a user to select one or more base nodes and limit the display of related nodes to those nodes within a defined degree of separation from the base node(s). The user can control the desired degree of separation, can select multiple base nodes, and can predetermine the base nodes to be used (and desired degree of separation for each) using a CSS-like language.

    摘要翻译： 提出了一种系统和方法，它将数据库中的图形数据存储为节点，链接节点的边缘和包含为节点和边缘提供附加上下文的属性的简档。 描述了基于存储在与每个节点相关的简档上的属性来动态创建图形数据的计算机化方法，包括预先确定在类似CSS的语言中使用哪些属性的能力。 还公开了用于视觉上聚焦于图形的一部分的方法，其允许用户选择一个或多个基本节点并且在与基本节点的限定的分离程度内限制相关节点的显示。 用户可以控制所需的分离度，可以选择多个基本节点，并且可以使用类似CSS的语言预先确定要使用的基本节点（以及每个节点的分离程度）。