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1.发明授权Guided self-assembly of block copolymer films on interferometrically nanopatterned substrates 有权引导自组装嵌段共聚物膜在干涉测量纳米图案衬底上公开(公告)号：US06746825B2
公开(公告)日：2004-06-08
申请号：US09971442
申请日：2001-10-05
申请人： Paul F. Nealey , Juan J. DePablo , Francesco Cerrina , Harun H. Solak , XiaoMin Yang , Richard D. Peters , Qiang Wang
发明人： Paul F. Nealey , Juan J. DePablo , Francesco Cerrina , Harun H. Solak , XiaoMin Yang , Richard D. Peters , Qiang Wang
IPC分类号： B05D306
CPC分类号： G03F7/26 , B82Y30/00 , G03F7/0002 , G03F7/165 , Y10S430/146 , Y10T156/10 , Y10T428/24058 , Y10T428/2995
摘要： Copolymer structures are formed by exposing a substrate with an imaging layer thereon to two or more beams of selected wavelengths to form interference patterns at the imaging layer to change the wettability of the imaging layer in accordance with the interference patterns. A layer of a selected block copolymer is deposited onto the exposed imaging layer and annealed to separate the components of the copolymer in accordance with the pattern of wettability and to replicate the pattern of the imaging layer in the copolymer layer. Stripes or isolated regions of the separated components may be formed with periodic dimensions in the range of 100 nm or less.
摘要翻译： 通过将其上的成像层的基板暴露于选定波长的两个或更多个波束以形成在成像层处的干涉图案以根据干涉图案改变成像层的润湿性而形成共聚物结构。 将选择的嵌段共聚物的层沉积到曝光的成像层上并退火，以根据润湿性的图案分离共聚物的组分并复制共聚物层中成像层的图案。 分离的组分的条带或隔离区域可以以100nm或更小的周期尺寸形成。
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公开(公告)号：US08097457B2
公开(公告)日：2012-01-17
申请号：US13085846
申请日：2011-04-13
申请人： Nicholas L. Abbott , Jeffrey C. Mohr , Christopher J. Murphy , Elise Huang , Juan J. DePablo , Nathan A. Lockwood , Sean P. Palecek
发明人： Nicholas L. Abbott , Jeffrey C. Mohr , Christopher J. Murphy , Elise Huang , Juan J. DePablo , Nathan A. Lockwood , Sean P. Palecek
IPC分类号： G01N33/53 , G01N33/567 , G01N33/566 , C12N5/00 , C12N5/02
CPC分类号： C12N5/0068 , C12N5/0606 , C12N2533/50 , C12N2533/52 , C12N2533/54 , C12N2533/90
摘要： The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.
摘要翻译： 本发明涉及可用于细胞培养的液晶底物及其使用方法。 在某些实施方案中，本发明提供了用于通过活细胞对细胞外基质组分的变化（例如重组）成像的方法和装置。
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公开(公告)号：US07947500B2
公开(公告)日：2011-05-24
申请号：US12777320
申请日：2010-05-11
申请人： Nicholas L. Abbott , Jeffrey C. Mohr , Christopher J. Murphy , Elise Huang , Juan J. DePablo , Nathan A. Lockwood , Sean P. Palecek
发明人： Nicholas L. Abbott , Jeffrey C. Mohr , Christopher J. Murphy , Elise Huang , Juan J. DePablo , Nathan A. Lockwood , Sean P. Palecek
IPC分类号： G01N33/53 , G01N33/567 , G01N33/566 , C12N5/00 , C12N5/02
CPC分类号： C12N5/0068 , C12N5/0606 , C12N2533/50 , C12N2533/52 , C12N2533/54 , C12N2533/90
摘要： The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.
摘要翻译： 本发明涉及可用于细胞培养的液晶底物及其使用方法。 在某些实施方案中，本发明提供了用于通过活细胞对细胞外基质组分的变化（例如重组）成像的方法和装置。
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4.发明申请公开(公告)号：US20090053268A1
公开(公告)日：2009-02-26
申请号：US11843303
申请日：2007-08-22
申请人： Juan J. DePablo , Mary Jo Biddy
发明人： Juan J. DePablo , Mary Jo Biddy
CPC分类号： C08L91/06 , A61K8/0241 , A61K8/92 , A61K2800/413 , A61K2800/651 , A61Q19/00 , B82Y5/00 , B82Y30/00 , C10M171/06 , C10M2201/061 , C10M2201/0613 , C10M2201/062 , C10M2201/0623 , C10M2201/08 , C10M2201/0803 , C10M2201/084 , C10M2201/085 , C10M2201/0853 , C10M2205/183 , C10M2207/10 , C10M2207/103 , C10M2207/401 , C10N2210/01 , C10N2210/02 , C11C5/002
摘要： The present invention provides compositions and products, such as waxes and lubricants, comprising a plurality of nanoparticles dispersed in a continuous phase comprising a vegetable oil derived material, such as one or more vegetable oils or a synthetic product derived from one or more vegetable oils. Incorporation of nanoparticles in the present compositions is beneficial for providing mechanical, thermal and/or chemical properties useful for a selected product or product application. In some compositions of the present invention, for example, incorporation of the nanoparticle component provides compositions derived from one or more vegetable oils exhibiting enhanced mechanical stability, hardness, viscosity, thermal stability and mechanical strength.
摘要翻译： 本发明提供组合物和产品，例如蜡和润滑剂，其包含分散在连续相中的多种纳米颗粒，所述连续相包含植物油衍生物质，例如一种或多种植物油或衍生自一种或多种植物油的合成产品。 在本发明组合物中纳入纳米颗粒有利于提供对所选产品或产品应用有用的机械，热和/或化学性质。 在本发明的一些组合物中，例如，纳米颗粒组分的掺入提供衍生自一种或多种植物油的组合物，其表现出增强的机械稳定性，硬度，粘度，热稳定性和机械强度。
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公开(公告)号：US20100197013A1
公开(公告)日：2010-08-05
申请号：US12615044
申请日：2009-11-09
CPC分类号： C07K16/18 , C12N5/0603 , C12N2533/90
摘要： A three-dimensional microwell system that supports long term pluripotent cell culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured pluripotent cells remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel®-coated, tissue culture-treated polystyrene dishes. Microwell-cultured pluripotent cells maintain pluripotency, differentiating to each of the three embryonic germ layers. Pluripotent cell aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain pluripotent cell growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct pluripotent cell differentiation by generating monodisperse EBs of a desired size and shape. Cardiomyocyte-rich EBs are obtained from pluripotent cells cultured in microwells of defined size and shape.
摘要翻译： 描述了支持长期多能细胞培养和形成均质胚状体（EB）的三维微孔系统。 微培养的多能细胞在培养物中保持存活和未分化数周，并在传播至经Matrigel®涂覆的组织培养处理的聚苯乙烯培养皿时保持未分化的复制。 微孔培养的多能细胞保持多能性，与三个胚胎胚层分化。 可以将从微孔释放的多能细胞聚集体传代为未分化复制或分化为单分散EB。 在三维中约束多能细胞生长的能力有利地提供了更有效，可重复的未分化细胞的培养，高通量筛选，以及通过产生所需大小和形状的单分散EB来引导多能细胞分化的能力。 富含心肌细胞的EB是从在规定尺寸和形状的微孔中培养的多能细胞获得的。
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公开(公告)号：US08956867B2
公开(公告)日：2015-02-17
申请号：US12615044
申请日：2009-11-09
CPC分类号： C07K16/18 , C12N5/0603 , C12N2533/90
摘要： A three-dimensional microwell system that supports long term pluripotent cell culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured pluripotent cells remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel®-coated, tissue culture-treated polystyrene dishes. Microwell-cultured pluripotent cells maintain pluripotency, differentiating to each of the three embryonic germ layers. Pluripotent cell aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain pluripotent cell growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct pluripotent cell differentiation by generating monodisperse EBs of a desired size and shape. Cardiomyocyte-rich EBs are obtained from pluripotent cells cultured in microwells of defined size and shape.
摘要翻译： 描述了支持长期多能细胞培养和形成均质胚状体（EB）的三维微孔系统。 微培养的多能细胞在培养物中保持存活和未分化数周，并在传播至经Matrigel®涂覆的组织培养处理的聚苯乙烯培养皿时保持未分化的复制。 微孔培养的多能细胞保持多能性，与三个胚胎胚层分化。 可以将从微孔释放的多能细胞聚集体传代为未分化复制或分化为单分散EB。 在三维中约束多能细胞生长的能力有利地提供了更有效，可重复的未分化细胞的培养，高通量筛选，以及通过产生所需大小和形状的单分散EB来引导多能细胞分化的能力。 富含心肌细胞的EB是从在规定尺寸和形状的微孔中培养的多能细胞获得的。
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公开(公告)号：US20080220520A1
公开(公告)日：2008-09-11
申请号：US12032986
申请日：2008-02-18
申请人： Sean P. Palecek , Juan J. DePablo , Jeffrey C. Mohr , Lin Ji
发明人： Sean P. Palecek , Juan J. DePablo , Jeffrey C. Mohr , Lin Ji
CPC分类号： A01N1/02 , A01N1/0221 , A01N1/0231
摘要： The present invention relates to methods and structures for preparing stem cells for use in cryopreservation methods. Stem cell colonies are provided between first and second matrix portions and are exposed to a carbohydrate-containing cryoprotecting medium and a freezing medium. The methods of the invention yield cryopreserved cells that maintain cell viability and exhibit limited cell differentiation after freezing and thawing, facilitating storage, shipping and handling of embryonic stem cell stocks and lines for research and therapeutics.
摘要翻译： 本发明涉及用于制备用于冷冻保存方法的干细胞的方法和结构。 提供干细胞集落在第一和第二基质部分之间，并暴露于含碳水化合物的低温保护培养基和冷冻培养基中。 本发明的方法产生维持细胞存活力的冷冻保存的细胞，并且在冷冻和融化后表现出有限的细胞分化，促进胚胎干细胞种群和用于研究和治疗的品系的储存，运输和处理。
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8.发明授权Guided self-assembly of block copolymer films on interferometrically nanopatterned substrates 有权引导自组装嵌段共聚物膜在干涉测量纳米图案衬底上公开(公告)号：US06926953B2
公开(公告)日：2005-08-09
申请号：US10800923
申请日：2004-03-15
申请人： Paul F. Nealey , Juan J. DePablo , Francesco Cerrina , Harun H. Solak , XiaoMin Yang , Richard D. Peters , Qiang Wang
发明人： Paul F. Nealey , Juan J. DePablo , Francesco Cerrina , Harun H. Solak , XiaoMin Yang , Richard D. Peters , Qiang Wang
CPC分类号： G03F7/26 , B82Y30/00 , G03F7/0002 , G03F7/165 , Y10S430/146 , Y10T156/10 , Y10T428/24058 , Y10T428/2995
摘要： Copolymer structures are formed by exposing a substrate with an imaging layer thereon to two or more beams of selected wavelengths to form interference patterns at the imaging layer to change the wettability of the imaging layer in accordance with the interference patterns. A layer of a selected block copolymer is deposited onto the exposed imaging layer and annealed to separate the components of the copolymer in accordance with the pattern of wettability and to replicate the pattern of the imaging layer in the copolymer layer. Stripes or isolated regions of the separated components may be formed with periodic dimensions in the range of 100 nm or less.
摘要翻译： 通过将其上的成像层的基板暴露于选定波长的两个或更多个波束以形成在成像层处的干涉图案以根据干涉图案改变成像层的润湿性而形成共聚物结构。 将选择的嵌段共聚物的层沉积到曝光的成像层上并退火，以根据润湿性的图案分离共聚物的组分并复制共聚物层中成像层的图案。 分离的组分的条带或隔离区域可以以100nm或更小的周期尺寸形成。
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