公开(公告)号：US11375900B2

公开(公告)日：2022-07-05

申请号：US16037183

申请日：2018-07-17

Applicant: Christopher J. Murphy ,  Nicholas L. Abbott ,  Jonathan McAnulty ,  Rishabh Jain

Inventor： Christopher J. Murphy ,  Nicholas L. Abbott ,  Jonathan McAnulty ,  Rishabh Jain

IPC: A61B5/00 ,  A61B90/30

Abstract: The present invention relates to methods and devices for characterizing wounds, and in particular to the use of Raman spectroscopy to characterize the state of healing of a wound.

公开(公告)号：US20210244676A1

公开(公告)日：2021-08-12

申请号：US17222089

申请日：2021-04-05

Applicant: Nicholas L. Abbott ,  Christopher J. Murphy

Inventor： Nicholas L. Abbott ,  Christopher J. Murphy

IPC: A61K9/70 ,  A61K48/00 ,  A61K9/00 ,  A61K45/06 ,  A61K31/00 ,  A61K31/095 ,  A61K31/375 ,  A61K31/66 ,  A61K31/70 ,  A61K31/7088 ,  A61K31/726 ,  A61K38/02 ,  A61K38/05

Abstract: The present invention relates to methods and compositions for modifying mucous membranes. In particular, the present invention relates to treating diseases associated with mucous membranes by changing the intrinsic chemical composition and/or physical features of a target mucous membrane.

公开(公告)号：US09012569B2

公开(公告)日：2015-04-21

申请号：US12354565

申请日：2009-01-15

Applicant: Nicholas L. Abbott ,  Francesco Caruso ,  Jugal K. Gupta ,  Sri Sivakumar

Inventor： Nicholas L. Abbott ,  Francesco Caruso ,  Jugal K. Gupta ,  Sri Sivakumar

IPC: C08F2/32 ,  G01N21/64 ,  G01N33/542 ,  G01N33/543

CPC classification number: G01N33/54373 ,  G01N21/6428 ,  Y10S514/938

Abstract: The invention provides oil emulsion droplets and a general and facile method for providing same through the use of templating multilayer capsules. The oil emulsion droplets are further useful in fabricating liquid crystal droplet-based biosensors for the detection of target analytes such as bacteria or viruses in a sample.

Abstract translation: 本发明提供油乳液液滴和通过使用模板化多层胶囊提供相同的通用和简便的方法。 油乳液液滴还可用于制造用于检测样品中目标分析物如细菌或病毒的液晶液滴生物传感器。

公开(公告)号：US08278040B2

公开(公告)日：2012-10-02

申请号：US12512362

申请日：2009-07-30

Applicant: Nicholas L. Abbott ,  Ankit Agarwal ,  Santanu K. Pal

Inventor： Nicholas L. Abbott ,  Ankit Agarwal ,  Santanu K. Pal

IPC: C12Q1/68

CPC classification number: C09K19/544 ,  C09K19/52 ,  Y10T428/10

Abstract: Colloidal liquid crystal gels (CLCGs), sensors incorporating the CLCGs, culture substrates made from the CLCGs, and patterned films and molded articles made from the CLCGs are provided. The CLCGs are composite liquid crystal materials comprising networks of particles having liquid crystal domains dispersed therein.

Abstract translation: 提供胶体液晶凝胶（CLCG），包含CLCG的传感器，由CLCG制成的培养基材，以及由CLCG制成的图案化膜和模塑制品。 CLCG是包含其中分散有液晶畴的颗粒网络的复合液晶材料。

公开(公告)号：US08021570B2

公开(公告)日：2011-09-20

申请号：US11762574

申请日：2007-06-13

Applicant: Samuel H. Gellman ,  Nicholas L. Abbott ,  William C. Pomerantz

Inventor： Samuel H. Gellman ,  Nicholas L. Abbott ,  William C. Pomerantz

IPC: C09K19/06 ,  C09K19/54 ,  B01J19/00 ,  G01N33/68 ,  C07K1/02

CPC classification number: C09K19/54 ,  A61K47/66 ,  B82Y5/00 ,  C09K19/02 ,  C09K19/389 ,  G01N33/68 ,  G01N33/6842 ,  G01N33/6851 ,  Y10S977/705

Abstract: The present invention provides materials and methods that make liquid crystal phases accessible with relatively short β-peptides in aqueous solvents.

Abstract translation: 本发明提供使液晶相在水性溶剂中可以用相对较短的＆amp; b-肽获得的材料和方法。

公开(公告)号：US07990488B2

公开(公告)日：2011-08-02

申请号：US12786317

申请日：2010-05-24

Applicant: Nicholas L. Abbott ,  Jeffrey M. Brake

Inventor： Nicholas L. Abbott ,  Jeffrey M. Brake

IPC: G02F1/13 ,  G02F1/1333 ,  G01N33/53 ,  G01N33/566 ,  G01N21/85

CPC classification number: C09K19/00 ,  C09K2019/528 ,  G01N33/54366 ,  Y10S436/805 ,  Y10T428/10 ,  Y10T428/1059 ,  Y10T428/1095

Abstract: A method of forming a liquid crystal device, includes: contacting an aqueous solution comprising a surfactant and a receptor molecule with a top surface of a liquid crystal. The liquid crystal is in a holding compartment of a substrate, and the receptor molecule is adsorbed on the top surface of the liquid crystal forming an interface between the liquid crystal and the aqueous solution. The receptor molecule is different than the surfactant. A method of detecting a compound in a flowing stream includes passing an aqueous solution over a top surface of a liquid crystal in a holding compartment of a substrate. The method also includes determining whether a change in the orientation of the liquid crystal occurs as the aqueous solution is passed over the top surface of the liquid crystal. A change in the orientation of the liquid crystal indicates the presence of the compound in the flowing stream.

Abstract translation: 形成液晶装置的方法包括：将包含表面活性剂和受体分子的水溶液与液晶的顶表面接触。 液晶位于基板的保持室中，受体分子被吸附在液晶的顶面上，形成液晶与水溶液的界面。 受体分子与表面活性剂不同。 在流动物流中检测化合物的方法包括将水溶液通过基板的保持室中的液晶的顶表面。 该方法还包括确定当水溶液通过液晶的顶表面时是否发生液晶的取向的变化。 液晶的取向的变化表示化合物在流动流中的存在。

公开(公告)号：US07947500B2

公开(公告)日：2011-05-24

申请号：US12777320

申请日：2010-05-11

Applicant: Nicholas L. Abbott ,  Jeffrey C. Mohr ,  Christopher J. Murphy ,  Elise Huang ,  Juan J. DePablo ,  Nathan A. Lockwood ,  Sean P. Palecek

Inventor： Nicholas L. Abbott ,  Jeffrey C. Mohr ,  Christopher J. Murphy ,  Elise Huang ,  Juan J. DePablo ,  Nathan A. Lockwood ,  Sean P. Palecek

IPC: G01N33/53 ,  G01N33/567 ,  G01N33/566 ,  C12N5/00 ,  C12N5/02

CPC classification number: C12N5/0068 ,  C12N5/0606 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

Abstract: The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.

Abstract translation: 本发明涉及可用于细胞培养的液晶底物及其使用方法。 在某些实施方案中，本发明提供了用于通过活细胞对细胞外基质组分的变化（例如重组）成像的方法和装置。

公开(公告)号：US07795007B2

公开(公告)日：2010-09-14

申请号：US11156911

申请日：2005-06-20

Applicant: Nicholas L. Abbott ,  Brian H. Clare ,  Paul J. Bertics

Inventor： Nicholas L. Abbott ,  Brian H. Clare ,  Paul J. Bertics

IPC: C12M1/34 ,  G01N33/553 ,  G01N33/547 ,  C07K1/10 ,  G01N33/53

CPC classification number: G01N33/542 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01J2219/00745 ,  B01J2219/0075 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00 ,  C40B40/06 ,  C40B40/10 ,  C40B40/18 ,  C40B60/14 ,  G01N33/54373 ,  G01N33/6842

Abstract: A method for differentiating between a post-translationally modified peptide and a peptide contained in a sample, comprising: (a) contacting the sample with a peptide attachment surface to create a peptidized surface, wherein the sample includes at least one functional group; (b) contacting the peptidized surface with a recognition reagent that selectively binds or forms a complex with the post-translationally modified peptide in the sample to provide an incubated surface; and (c) contacting a liquid crystal with the incubated surface and detecting presence of post-translationally modified peptide in the sample with the liquid crystal.

Abstract translation: 一种用于区分样品中包含的翻译后修饰的肽和肽的方法，包括：（a）使样品与肽附着表面接触以产生肽化表面，其中所述样品包括至少一个官能团; （b）使肽化表面与识别试剂接触，所述识别试剂选择性地与样品中的翻译后修饰的肽结合或形成复合物以提供孵育的表面; 和（c）使液晶与孵育的表面接触并用液晶检测样品中翻译后修饰的肽的存在。

公开(公告)号：US07732152B2

公开(公告)日：2010-06-08

申请号：US11565363

申请日：2006-11-30

Applicant: Nicholas L. Abbott ,  Jeffrey C. Mohr ,  Christopher J. Murphy ,  Elise Huang ,  Juan J. De Pablo ,  Sean P. Palecek ,  Nathan A. Lockwood

Inventor： Nicholas L. Abbott ,  Jeffrey C. Mohr ,  Christopher J. Murphy ,  Elise Huang ,  Juan J. De Pablo ,  Sean P. Palecek ,  Nathan A. Lockwood

IPC: G01N33/53 ,  G01N33/567 ,  C12N5/00 ,  C12N5/02 ,  G01N33/566

CPC classification number: C12N5/0068 ,  C12N5/0606 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

Abstract: The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.

Abstract translation: 本发明涉及可用于细胞培养的液晶底物及其使用方法。 在某些实施方案中，本发明提供了用于通过活细胞对细胞外基质组分的变化（例如重组）成像的方法和装置。

公开(公告)号：US06692699B2

公开(公告)日：2004-02-17

申请号：US09784679

申请日：2001-02-15

Applicant: Nicholas L. Abbott ,  Seung-Ryeol Kim

Inventor： Nicholas L. Abbott ,  Seung-Ryeol Kim

IPC: G01N2147

CPC classification number: G01N33/54366 ,  G01N33/54353 ,  G01N33/54373 ,  G01N33/552 ,  Y10T428/1018

Abstract: A rubbed substrate structure for use in a liquid crystal assay device, includes: a biochemical blocking compound chemically immobilized on a surface of one side of a support forming a biochemical blocking layer; and a biomolecule recognition agent deposited on the side of the support containing the biochemical blocking layer. The biomolecule recognition agent includes a recognition site capable of selectively recognizing a target species to be detected by the liquid crystal assay device. Additionally, the surface of the side of the support containing the biochemical blocking layer is rubbed such that it possesses features that drive a uniform anchoring of liquid crystals when the liquid crystals contact the side of the support containing the biochemical blocking layer. A method for preparing a rubbed substrate structure for use in a liquid crystal assay device, optical cells for use in a liquid crystal assay, liquid crystal assay devices, kits for use in a liquid crystal assay, and methods for detecting the presence of a target species using a liquid crystal assay device are also disclosed.

Abstract translation: 用于液晶测定装置的摩擦衬底结构包括：化学固定在形成生化阻断层的支持物一侧的表面上的生化封闭化合物; 和沉积在含有生化阻断层的载体侧上的生物分子识别剂。 生物分子识别剂包括能够选择性地识别由液晶测定装置检测的靶物种的识别位点。 此外，含有生化阻断层的载体的表面被摩擦，使得其具有当液晶接触包含生化阻断层的载体侧时驱动液晶的均匀锚定的特征。 一种制备用于液晶测定装置的摩擦衬底结构的方法，用于液晶测定的光学单元，液晶测定装置，用于液晶测定的试剂盒以及用于检测靶的存在的方法 还公开了使用液晶测定装置的物种。