公开(公告)号：US20100290999A1

公开(公告)日：2010-11-18

申请号：US12576193

申请日：2009-10-08

申请人： Sehoon KIM ,  Ick Chan KWON ,  Kwangmeyung KIM ,  Kuiwon CHOI ,  Chang-Keun LIM

发明人： Sehoon KIM ,  Ick Chan KWON ,  Kwangmeyung KIM ,  Kuiwon CHOI ,  Chang-Keun LIM

IPC分类号： A61K49/00 ,  C08G14/02 ,  C12Q1/02

CPC分类号： A61K49/0019 ,  A61K49/0093

摘要： Disclosed are nanoparticles of a light emissive polymer, comprising nanoparticles of a cyano-substituted poly(arylene vinylene) polymer; and a biocompatible surfactant adsorbed to the surface of the nanoparticles of the polymer, and preparation method thereof, wherein the method comprises: (1) uniformly mixing a dialdehyde monomer represented by a general formula OHC—Ar1—CHO, a dicyanide monomer represented by a general formula NC—Ar2—CN, and a liquid surfactant; (2) adding water to the resulting mixture to prepare an aqueous micelle dispersion; and (3) adding a polymerization catalyst to the aqueous micelle dispersion, followed by carrying out colloidal polymerization of the resulting mixture at room temperature under an atmosphere. The nanoparticles of the light emissive polymer of the invention are stabilized with a biocompatible surfactant, so that it can form a stable aqueous dispersion phase, and has particle size and fluorescence efficiency suitable for a biomolecular marker or a cell or in vivo imaging; therefore, it can be used as a cell or in vivo light emission contrast agent.

摘要翻译： 公开了包含氰基取代的聚（亚芳基亚乙烯基）聚合物的纳米颗粒的发光聚合物的纳米颗粒; 和吸附在聚合物纳米颗粒表面的生物相容性表面活性剂及其制备方法，其中所述方法包括：（1）均匀混合由通式OHC-Ar1-CHO表示的二醛单体，由 通式NC-Ar2-CN，和液体表面活性剂; （2）向所得混合物中加入水以制备水性胶束分散体; 和（3）将聚合催化剂加入到水性胶束分散体中，然后在室温下在气氛下进行所得混合物的胶体聚合。 本发明的光发射聚合物的纳米颗粒用生物相容的表面活性剂稳定，从而可以形成稳定的水分散相，并具有适用于生物分子标记或细胞或体内成像的粒度和荧光效率; 因此，它可以用作细胞或体内发光造影剂。

公开(公告)号：US20120083455A1

公开(公告)日：2012-04-05

申请号：US13245180

申请日：2011-09-26

申请人： Kwangmeyung KIM ,  Ick Chan KWON ,  Kuiwon CHOI ,  In Chan YOUN ,  Myung Sook HUH ,  Sojin LEE

发明人： Kwangmeyung KIM ,  Ick Chan KWON ,  Kuiwon CHOI ,  In Chan YOUN ,  Myung Sook HUH ,  Sojin LEE

IPC分类号： A61K38/38 ,  A61P35/00 ,  C07K14/765

CPC分类号： C12N15/111 ,  A61K47/643 ,  C12N2320/32

摘要： Disclosed are a human serum albumin-siRNA carrier system having siRNA bound to human serum albumin and a user thereof, and especially, human serum albumin-siRNA carrier system, which has a biodegradable covalent bond between human serum albumin polymer and siRNA and is stable in a living body, and a user thereof.The human serum albumin-siRNA carrier system having the biodegradable covalent bond between the human serum albumin and the siRNA exhibits high siRNA delivery efficiency to a target site in the living body. Therefore, the human serum albumin-siRNA carrier system may allow siRNA for therapy to be efficiently delivered to a target site such as cancer tissues in the living body even by being administrated in a relatively low concentration, which may result in a wide use for therapies of various diseases.

摘要翻译： 公开了具有与人血清白蛋白结合的siRNA及其使用者的人血清白蛋白-siRNA载体体系，特别是人血清白蛋白-siRNA载体体系，其在人血清白蛋白聚合物和siRNA之间具有可生物降解的共价键，并且在 生物体及其使用者。 在人体血清白蛋白和siRNA之间具有可生物降解的共价键的人血清白蛋白-siRNA载体体系对生物体中的靶位点显示出高的siRNA递送效率。 因此，人体血清白蛋白-siRNA载体体系可以使siRNA治疗有效地递送到目标部位如生物体中的癌组织，甚至通过以相对低的浓度施用，这可能导致广泛用于治疗 的各种疾病。

公开(公告)号：US20100310468A1

公开(公告)日：2010-12-09

申请号：US12792186

申请日：2010-06-02

申请人： Hyung Jun AHN ,  Ick Chan KWON ,  Kuiwon CHOI ,  Kwangmyeng KIM ,  Inchan YOUN ,  Sehoon KIM

发明人： Hyung Jun AHN ,  Ick Chan KWON ,  Kuiwon CHOI ,  Kwangmyeng KIM ,  Inchan YOUN ,  Sehoon KIM

IPC分类号： C07K14/76 ,  A61K47/48 ,  C12N15/81 ,  C12N1/19 ,  C12P21/02 ,  A61K49/00

CPC分类号： C07K14/76 ,  A61K47/643 ,  C07K14/765 ,  C07K2319/20

摘要： The present invention relates to recombinant albumins fused with poly-cysteine peptide and methods for preparing the same, more precisely recombinant albumins in which cysteines that can be used for drug binding are amplified at N-terminal and C-terminal of the albumin and methods for preparing the same. The recombinant albumin of the present invention demonstrates improved albumin-drug conjugation efficiency when it is used for drug delivery system, indicating that it can effectively deliver a large amount of drug to a target tissue. At the same time, the recombinant albumin of the present invention can be used as an excellent drug deliverer with reduced side effects, compared with the conventional albumin carriers, by regulating the amount of drug conjugated to each unit of albumin by regulating the number of cysteine fused thereto. In addition, the recombinant albumin of the present invention can be used for the screening of a novel drug and for the non-invasive real-time diagnosis and treatment of disease by combining with a fluorescent material or a contrast agent for molecular imaging.

摘要翻译： 本发明涉及与多聚半胱氨酸肽融合的重组白蛋白及其制备方法，更准确地说，重组白蛋白，其中可用于药物结合的半胱氨酸在白蛋白的N末端和C末端扩增， 准备一样 本发明的重组白蛋白在用于药物递送系统时表现出改善的白蛋白 - 药物共轭效率，表明其可有效地将大量药物递送至靶组织。 同时，与常规白蛋白载体相比，本发明的重组白蛋白可以用作优异的药物递送剂，通过调节半胱氨酸数目调节与每单位白蛋白结合的药物的量 融合。 此外，本发明的重组白蛋白可以用于筛选新药和用于分子成像的荧光材料或造影剂的非侵入性实时诊断和治疗疾病。

公开(公告)号：US20120065242A1

公开(公告)日：2012-03-15

申请号：US12905212

申请日：2010-10-15

申请人： Kwang Meyung KIM ,  Ick Chan KWON ,  Kuiwon CHOI ,  Myung Sook HUH ,  Seung Young LEE ,  So Jin LEE

发明人： Kwang Meyung KIM ,  Ick Chan KWON ,  Kuiwon CHOI ,  Myung Sook HUH ,  Seung Young LEE ,  So Jin LEE

IPC分类号： A61K31/713 ,  A61P35/00 ,  C07H21/04 ,  B82Y5/00

CPC分类号： A61K47/4823 ,  A61K47/61 ,  A61K47/6939 ,  C12N15/111 ,  C12N15/87 ,  C12N2310/14 ,  C12N2310/351 ,  C12N2320/32

摘要： Disclosed is a polymer-siRNA delivery carrier in which a siRNA is combined with a polymer and the use thereof. More specifically, there is disclosed a stable in vivo polymer-siRNA delivery carrier in which a polymer and a siRNA are combined by using charge interaction and biodegradable covalent bonding at the same time and the use thereof.The polymer-siRNA delivery carrier in which a polymer and a siRNA are combined by using charge interaction and biodegradable covalent bonding at the same time has a high siRNA deliver efficiency to a target portion in vivo. Hence, according to the polymer-siRNA binder, the siRNA for treatment can be effectively delivered to a target portion such as in vivo cancer tissue, and the like even with administration of a relatively low concentration, and thus widely used for the treatment of various kinds of diseases.

摘要翻译： 公开了将siRNA与聚合物组合的聚合物-siRNA递送载体及其用途。 更具体地，公开了一种稳定的体内聚合物-siRNA递送载体，其中通过使用电荷相互作用和可生物降解的共价键在同一时间将聚合物和siRNA组合起来，以及其用途。 通过使用电荷相互作用和可生物降解的共价键合将聚合物和siRNA组合的聚合物-siRNA递送载体在体内对目标部分具有高的siRNA递送效率。 因此，根据聚合物-siRNA粘合剂，即使施用相对低的浓度，也可以将治疗用siRNA有效地输送到诸如体内癌组织等目标部位，广泛用于各种治疗 各种疾病

公开(公告)号：US20070148770A1

公开(公告)日：2007-06-28

申请号：US11565541

申请日：2006-11-30

申请人： Seo Young JEONG ,  Eunhee BAE ,  Ick Chan KWON ,  Kuiwon CHOI

发明人： Seo Young JEONG ,  Eunhee BAE ,  Ick Chan KWON ,  Kuiwon CHOI

IPC分类号： C12N5/08 ,  C12N5/00

CPC分类号： C12N5/0075 ,  C12N2533/72

摘要： The present invention relates to macroporous chitosan beads having 5-200 μm in size of relatively large and uniform pores that are distributed from surface to core region. The macroporous chitosan beads of the present invention make cell culturing efficient. Cells can attach to them efficiently due to their large surface area.

摘要翻译： 本发明涉及从表面到核心区域分布的具有相对较大且均匀的孔的尺寸为5-200μm的大孔壳聚糖珠粒。 本发明的大孔壳聚糖珠使细胞培养有效。 由于表面积较大，细胞可以有效地附着在细胞上。

公开(公告)号：US20100209353A1

公开(公告)日：2010-08-19

申请号：US12409479

申请日：2009-03-23

申请人： Ick Chan KWON ,  Kwang Meyung KIM ,  Kuiwon CHOI

发明人： Ick Chan KWON ,  Kwang Meyung KIM ,  Kuiwon CHOI

IPC分类号： A61K49/00 ,  C07K14/76 ,  B32B5/12 ,  A61K9/14 ,  A61K47/00

CPC分类号： A61K49/0056 ,  A61K9/5123 ,  A61K9/5169 ,  A61K31/00 ,  A61K47/6907 ,  A61K47/6925 ,  A61K49/0032 ,  A61K49/0093 ,  A61K49/143 ,  Y10T428/2982

摘要： The present invention relates to a protein conjugate having excellent tumor targeting capacity and a method for preparing the same, more precisely albumin-bile acid conjugate forming 30-500 nm nano-particles and forming self-aggregates in water system and a method for preparing the same. The albumin-bile acid conjugate of the present invention has excellent tumor targeting capacity and facilitates inclusion of a hydrophobic anticancer agent and can be combined with fore infrared ray fluorescent material. Therefore, this conjugate can be effectively used for the production of a novel nano-particle contrast agent or a nano-particle drug delivery system for the diagnosis and treatment of cancer.

摘要翻译： 本发明涉及具有优异的肿瘤靶向能力的蛋白质缀合物及其制备方法，更准确地说，形成30-500nm纳米颗粒的白蛋白 - 胆汁酸缀合物并在水系中形成自聚集体，以及制备 相同。 本发明的白蛋白 - 胆汁酸结合物具有优异的肿瘤靶向能力，并且促进包含疏水性抗癌剂并且可以与前置红外线荧光材料组合。 因此，该缀合物可有效地用于生产用于诊断和治疗癌症的新型纳米颗粒造影剂或纳米颗粒药物递送系统。