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公开(公告)号：US20100323409A1
公开(公告)日：2010-12-23
申请号：US12819532
申请日：2010-06-21
申请人： Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Olga Sergeevna Beznoschenko
发明人： Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Olga Sergeevna Beznoschenko
CPC分类号： C12N9/0069 , C12P13/06
摘要： A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using an L-isoleucine-producing bacterium transformed with a DNA fragment containing a gene coding for a protein having L-isoleucine dioxygenase activity; and having the ability to produce (2S,3R,4S)-4-hydroxy-L-isoleucine.
摘要翻译： 使用由含有编码具有L-异亮氨酸双加氧素活性的蛋白质的基因的DNA片段转化的L-异亮氨酸生产菌的（2S，3R，4S）-4-羟基-L-异亮氨酸或其盐的制造方法， 并具有生产（2S，3R，4S）-4-羟基-L-异亮氨酸的能力。
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2.发明授权Bacterium producing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity and a method for manufacturing the product 有权产生由具有2-氧戊二酸依赖性酶活性的蛋白质催化的反应产物的细菌和制备该产物的方法公开(公告)号：US08524476B2
公开(公告)日：2013-09-03
申请号：US12819485
申请日：2010-06-21
申请人： Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Olga Sergeevna Beznoschenko , Tatyana Aleksandrovna Bachina , Yuki Imabayashi , Masakazu Sugiyama , Shunichi Suzuki
发明人： Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Olga Sergeevna Beznoschenko , Tatyana Aleksandrovna Bachina , Yuki Imabayashi , Masakazu Sugiyama , Shunichi Suzuki
CPC分类号： C12P13/06 , C12N9/0069 , C12N9/0071 , C12P13/24
摘要： A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.
摘要翻译： 使用由DNA片段转化的细菌制造由具有2-氧戊二酸依赖性酶活性的蛋白质（例如（2S，3R，4S）-4-羟基-L-异亮氨酸或其盐）催化的反应产物的方法 含有编码具有2-氧戊二酸依赖性酶活性的蛋白质的基因，例如L-异亮氨酸双加氧酶活性; 并且其中所述细菌具有产生如（2S，3R，4S）-4-羟基-L-异亮氨酸的产物的能力。
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3.发明申请BACTERIUM PRODUCING A PRODUCT OF A REACTION CATALYZED BY A PROTEIN HAVING 2-OXOGLUTARATE-DEPENDENT ENZYME ACTIVITY AND A METHOD FOR MANUFACTURING THE PRODUCT 有权生产由具有2-羟基脂肪酸依赖性酶活性的蛋白质催化的反应产物的细菌和制造该产品的方法公开(公告)号：US20100330622A1
公开(公告)日：2010-12-30
申请号：US12819485
申请日：2010-06-21
申请人： Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Olga Sergeevna Beznoschenko , Tatyana Aleksandrovna Bachina , Yuki Imabayashi , Masakazu Sugiyama , Shunichi Suzuki
发明人： Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Olga Sergeevna Beznoschenko , Tatyana Aleksandrovna Bachina , Yuki Imabayashi , Masakazu Sugiyama , Shunichi Suzuki
CPC分类号： C12P13/06 , C12N9/0069 , C12N9/0071 , C12P13/24
摘要： A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.
摘要翻译： 使用由DNA片段转化的细菌制造由具有2-氧戊二酸依赖性酶活性的蛋白质（例如（2S，3R，4S）-4-羟基-L-异亮氨酸或其盐）催化的反应产物的方法 含有编码具有2-氧戊二酸依赖性酶活性的蛋白质的基因，例如L-异亮氨酸双加氧酶活性; 并且其中所述细菌具有产生如（2S，3R，4S）-4-羟基-L-异亮氨酸的产物的能力。
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公开(公告)号：US08273562B2
公开(公告)日：2012-09-25
申请号：US13344110
申请日：2012-01-05
申请人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Vitaly Grigorievich Paraskevov, legal representative , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
发明人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
CPC分类号： C12N9/0069 , C12P13/06
摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
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公开(公告)号：US08367381B2
公开(公告)日：2013-02-05
申请号：US13344170
申请日：2012-01-05
申请人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Sakayu Shimizu , Jun Ogawa , Makoto Hibi , Vitaly Grigorievich Paraskevov
发明人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
CPC分类号： C12N9/0069 , C12P13/06
摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
摘要翻译： 提供了一种来自苏云金芽孢杆菌的高活性L-异亮氨酸双加氧酶。 在L-异亮氨酸双加氧酶存在下，通过使L-异亮氨酸在水性溶剂中反应，并分离（2S，3R，4S） - 还提供了4-羟基-L-异亮氨酸。
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公开(公告)号：US20120107883A1
公开(公告)日：2012-05-03
申请号：US13344170
申请日：2012-01-05
申请人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Vitaly Grigorievich Paraskevov , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
发明人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Vitaly Grigorievich Paraskevov , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
IPC分类号： C12P13/06
CPC分类号： C12N9/0069 , C12P13/06
摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
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公开(公告)号：US20120107884A1
公开(公告)日：2012-05-03
申请号：US13344200
申请日：2012-01-05
申请人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Vitaly Grigorievich Paraskevov , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
发明人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Vitaly Grigorievich Paraskevov , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
IPC分类号： C12P13/06
CPC分类号： C12N9/0069 , C12P13/06
摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
摘要翻译： 提供了一种来自苏云金芽孢杆菌的高活性L-异亮氨酸双加氧酶。 在L-异亮氨酸双加氧酶存在下，通过使L-异亮氨酸在水性溶剂中反应，并分离（2S，3R，4S） - 还提供了4-羟基-L-异亮氨酸。
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公开(公告)号：US08367382B2
公开(公告)日：2013-02-05
申请号：US13344200
申请日：2012-01-05
申请人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Sakayu Shimizu , Jun Ogawa , Makoto Hibi , Vitaly Grigorievich Paraskevov
发明人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
CPC分类号： C12N9/0069 , C12P13/06
摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
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公开(公告)号：US20120115203A1
公开(公告)日：2012-05-10
申请号：US13344110
申请日：2012-01-05
申请人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Sakayu Shimizu , Jun Ogawa , Makoto Hibi , Vitaly Grigorievich Paraskevov
发明人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Sakayu Shimizu , Jun Ogawa , Makoto Hibi , Vitaly Grigorievich Paraskevov
CPC分类号： C12N9/0069 , C12P13/06
摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
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公开(公告)号：US08114651B2
公开(公告)日：2012-02-14
申请号：US12412823
申请日：2009-03-27
申请人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Vitaly Grigorievich Paraskevov, legal representative , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
发明人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
CPC分类号： C12N9/0069 , C12P13/06
摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
摘要翻译： 提供了一种来自苏云金芽孢杆菌的高活性L-异亮氨酸双加氧酶。 在L-异亮氨酸双加氧酶存在下，通过使L-异亮氨酸在水性溶剂中反应，并分离（2S，3R，4S） - 还提供了4-羟基-L-异亮氨酸。
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