公开(公告)号：US5840510A

公开(公告)日：1998-11-24

申请号：US885176

申请日：1997-06-30

申请人： Shigenori Tanaka ,  Hiroshi Tamura ,  Kazuhiro Aita

发明人： Shigenori Tanaka ,  Hiroshi Tamura ,  Kazuhiro Aita

IPC分类号： G01N33/543 ,  G01N33/579 ,  C12Q1/37 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/56

CPC分类号： G01N33/579 ,  G01N2333/811 ,  Y10S435/967

摘要： This invention provides (1) a reagent for endotoxin assay which comprises aprotinin and a limulus amebocyte lysate reagent, (2) a kit for endotoxin assay which comprises the limulus amebocyte lysate reagent and a reagent containing aprotinin, (3) a method for assaying endotoxin in a sample using the limulus amebocyte lysate reagent in which aprotinin is added to the lysate reagent and/or the sample, (4) a method for assaying endotoxin in a serine protease-containing sample using the limulus amebocyte lysate reagent in which the sample is allowed to contact with an aprotinin-immobilized insoluble carrier in advance of endotoxin assay, (5) a carrier for pretreating a serine protease-containing sample on which aprotinin is immobilized, (6) a method for inhibiting factor G activation in which aprotinin is added to the limulus amebocyte lysate reagent and (7) a factor G activation inhibitor which comprises aprotinin as an active ingredient. Endotoxin assay can be effected based on the factor C system reaction, without influences of factor G contained in the limulus amebocyte lysate reagent and/or serine proteases contained in samples.

摘要翻译： 本发明提供（1）含有抑肽酶和鲎变形细胞裂解物试剂的内毒素试剂，（2）包含鲎变形细胞裂解物试剂和含有抑肽酶的试剂的内毒素试剂盒，（3）检测内毒素的方法 在使用鲎变形细胞裂解物试剂的样品中，其中将抑肽酶加入到裂解物试剂和/或样品中，（4）使用鲎变形细胞裂解物试剂测定含丝氨酸蛋白酶样品中的内毒素的方法，其中样品是 在内毒素测定之前允许与抑肽酶固定的不溶性载体接触，（5）用于预处理含有抑肽酶的丝氨酸蛋白酶的样品的载体，（6）抑制因子G激活的方法，其中加入抑肽酶 到鲎变形细胞裂解物试剂和（7）包含抑肽酶作为活性成分的因子G激活抑制剂。 内毒素测定可以基于因子C系统反应进行，而不受样品中包含的鲎变形细胞裂解物试剂和/或丝氨酸蛋白酶中所含因子G的影响。

公开(公告)号：US5695948A

公开(公告)日：1997-12-09

申请号：US661705

申请日：1996-06-11

申请人： Shigenori Tanaka ,  Hiroshi Tamura ,  Kazuhiro Aita

发明人： Shigenori Tanaka ,  Hiroshi Tamura ,  Kazuhiro Aita

IPC分类号： G01N33/543 ,  G01N33/579 ,  C12Q1/56 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/44

CPC分类号： G01N33/579 ,  G01N2333/811 ,  Y10S435/967

摘要： This invention provides (1) a reagent for endotoxin assay which comprises aprotinin and a limulus amebocyte lysate reagent, (2) a kit for endotoxin assay which comprises the limulus amebocyte lysate reagent and a reagent containing aprotinin, (3) a method for assaying endotoxin in a sample using the limulus amebocyte lysate reagent in which aprotinin is added to the lysate reagent and/or the sample, (4) a method for assaying endotoxin in a serine protease-containing sample using the limulus amebocyte lysate reagent in which the sample is allowed to contact with an aprotinin-immobilized insoluble carrier in advance of endotoxin assay, (5) a carrier for pretreating a serine protease-containing sample on which aprotinin is immobilized, (6) a method for inhibiting factor G activation in which aprotinin is added to the limulus amebocyte lysate reagent and (7) a factor G activation inhibitor which comprises aprotinin as an active ingredient. Endotoxin assay can be effected based on the factor C system reaction, without influences of factor G contained in the limulus amebocyte lysate reagent and/or serine proteases contained in samples.

摘要翻译： 本发明提供（1）含有抑肽酶和鲎变形细胞裂解物试剂的内毒素试剂，（2）包含鲎变形细胞裂解物试剂和含有抑肽酶的试剂的内毒素试剂盒，（3）检测内毒素的方法 在使用鲎变形细胞裂解物试剂的样品中，其中将抑肽酶加入到裂解物试剂和/或样品中，（4）使用鲎变形细胞裂解物试剂测定含丝氨酸蛋白酶样品中的内毒素的方法，其中样品是 在内毒素测定之前允许与抑肽酶固定的不溶性载体接触，（5）用于预处理含有抑肽酶的丝氨酸蛋白酶的样品的载体，（6）抑制因子G激活的方法，其中加入抑肽酶 到鲎变形细胞裂解物试剂和（7）包含抑肽酶作为活性成分的因子G激活抑制剂。 内毒素测定可以基于因子C系统反应进行，而不受样品中包含的鲎变形细胞裂解物试剂和/或丝氨酸蛋白酶中所含因子G的影响。

公开(公告)号：US5641643A

公开(公告)日：1997-06-24

申请号：US542699

申请日：1995-10-13

申请人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

发明人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

IPC分类号： C07H3/06 ,  C08B37/00 ,  G01N33/579 ,  C12Q1/37 ,  C08B37/18 ,  C12Q1/34

CPC分类号： C07H3/06 ,  C08B37/0024 ,  G01N33/579

摘要： This invention relates to a horseshoe crab amebocyte lysate factor G activation inhibitor comprising as an active ingredient a polyglycoside containing at least one poly-(1.fwdarw.3)-.beta.-D-glucoside structure portion consisting of 2 to 370 (1.fwdarw.3)-.beta.-D-glucoside structural units of the following formula ##STR1## which are continuously bound to one another. This inhibitor is useful for inhibiting the activation of factor G which may exist in horseshoe crab amebocyte lysate used in the Limulus test.

摘要翻译： 本发明涉及一种马蹄形褐飞虱细胞裂解物因子G活化抑制剂，其包含作为活性成分的含有至少一种由2至370（1-> 3）组成的聚（1-> 3）-β-D-葡萄糖苷结构部分的多糖苷 ）-β-D-葡萄糖苷结构单元，其结构彼此连续。 该抑制剂可用于抑制鲎试验中使用的马蹄蟹血小板裂解物中可能存在的因子G的活化。

公开(公告)号：US5286625A

公开(公告)日：1994-02-15

申请号：US789583

申请日：1991-11-08

申请人： Shigenori Tanaka ,  Hiroshi Tamura

发明人： Shigenori Tanaka ,  Hiroshi Tamura

IPC分类号： G01N33/579 ,  C12Q1/34 ,  C12Q1/00 ,  C12Q1/37 ,  G01N1/00

CPC分类号： G01N33/579 ,  Y10T436/25125

摘要： A method of assaying an endotoxin using the limulus amebocyte lysate component which comprises pretreating whole blood with nitric acid and a surfactant selected from among polyoxyethylene ethers represented by the following formula: ##STR1## wherein n is an integer of from 8 to 40; or: ##STR2## polyoxyethylene sorbitans, n-alkylglucopyranosides, sodium dodecylsulfate and lithium dodecylsulfate, which enables an efficient and accuate assay of an endotoxin.

摘要翻译： 使用鲎变形细胞裂解物成分测定内毒素的方法，其包括用硝酸预处理全血和选自下式表示的聚氧乙烯醚中的表面活性剂：（*化学结构*）其中n为8〜40的整数 ; 或：（*化学结构*）聚氧乙烯脱水山梨糖醇酐，正烷基吡喃糖苷，十二烷基硫酸钠和十二烷基硫酸钠，其能够有效地进行内毒素测定。

公开(公告)号：US6156519A

公开(公告)日：2000-12-05

申请号：US793679

申请日：1997-06-02

申请人： Hiroshi Tamura ,  Shigenori Tanaka

发明人： Hiroshi Tamura ,  Shigenori Tanaka

IPC分类号： C07K14/435 ,  C07K16/18 ,  G01N33/579 ,  G01N33/53

CPC分类号： G01N33/579 ,  C07K14/43509 ,  C07K16/18 ,  G01N2400/24 ,  Y10S435/962 ,  Y10S530/857

摘要： A (1.fwdarw.3)-.beta.-D-glucan binding protein obtainable by affinity chromatography or gel filtration of an extract of horseshoe crab amoebocytes and having a molecular weight of about 580 kDa, as determined by gel filtration under non-reducing conditions, and about 170 kDa, as determined by SDS-PAGE under reducing conditions, is provided.An antibody which selectively recognizes the protein is also provided. Methods for detecting or removing (1.fwdarw.3)-.beta.-D-glucan in or from a sample using the protein and antibody are disclosed.

摘要翻译： PCT No.PCT / JP95 / 01735 Sec。 371日期：1997年6月2日 102（e）日期1997年6月2日PCT提交1995年8月31日PCT公布。 出版物WO96 / 06858 日期：1996年3月7日A（1-> 3）-β-D-葡聚糖结合蛋白，可通过亲和层析或凝胶过滤马蹄蟹变形细胞提取物获得，分子量约580kDa，通过凝胶过滤测定 提供非还原条件，约170kDa，通过SDS-PAGE在还原条件下测定。 还提供了选择性识别蛋白质的抗体。 公开了使用蛋白质和抗体检测或从样品中除去（1-> 3）-β-D-葡聚糖的方法。

公开(公告)号：US5681710A

公开(公告)日：1997-10-28

申请号：US391097

申请日：1995-02-21

申请人： Shigenori Tanaka ,  Hiroshi Tamura ,  Makoto Ohki

发明人： Shigenori Tanaka ,  Hiroshi Tamura ,  Makoto Ohki

IPC分类号： G01N33/579 ,  C12Q1/56 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/44

CPC分类号： G01N33/579 ,  G01N2400/22 ,  G01N2400/24

摘要： The present invention provides a reagent for determining (1.fwdarw.3)-.beta.-D-glucan comprising a lysate substantially free from any endotoxin-sensitive factor, which is obtained by contacting limulus amebocyte lysate optionally containing dextran with a polyamide or cellulose insoluble carrier, which makes it possible to rapidly and easily determine at a high accuracy (1.fwdarw.3)-.beta.-D-glucan of mycotic origin contained in the body fluid such as blood or urine.

摘要翻译： 本发明提供了一种用于测定（1→3）-β-D-葡聚糖的试剂，其包含基本上不含任何内毒素敏感因子的裂解物，所述裂解物通过任选地含有葡聚糖的鲎变形细胞裂解物与聚酰胺或纤维素不溶性载体 ，这使得可以以高精度（1→3）-β-D-葡聚糖快速和容易地测定包含在体液如血液或尿液中的真菌来源。

公开(公告)号：US5155032A

公开(公告)日：1992-10-13

申请号：US474057

申请日：1990-05-01

申请人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

发明人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

IPC分类号： C07H3/06 ,  C08B37/00 ,  G01N33/579

CPC分类号： C08B37/0024 ,  C07H3/06 ,  G01N33/579

摘要： This invention relates to a horseshoe crab amebocyte lysate factor G activation inhibitor comprising as an active ingredient a polyglycoside containing at least one poly-(1.fwdarw.3)-.beta.-D-glucoside structure portion consisting of 2 to 370 (1.fwdarw.3)-.beta.-D-glucoside structural units of the following formula ##STR1## which are continuously bound to one another. This inhibitor is useful for inhibiting the activation of factor G which may exist in horseshoe crab amebocyte lysate used in the Limulus test.

摘要翻译： PCT No.PCT / JP89 / 00903 Sec。 371日期1990年5月1日 102（e）日期1990年5月1日PCT提交1989年9月1日PCT公布。 公开号WO90 / 02951 日本3月22日，1990年。本发明涉及一种马蹄形蟹细胞裂解物因子G激活抑制剂，其包含含有至少一种由（2）〜（1→3）-β-D-葡萄糖苷结构部分组成的多糖苷的多糖苷作为活性成分 到具有连续结合的下式（IMAGE）的370（1-> 3）-β-D-葡萄糖苷结构单元。 该抑制剂可用于抑制鲎试验中使用的马蹄蟹血小板裂解物中可能存在的因子G的活化。

公开(公告)号：US5876955A

公开(公告)日：1999-03-02

申请号：US774236

申请日：1996-12-27

申请人： Hiroshi Tamura ,  Shigenori Tanaka ,  Jun Aketagawa ,  Toshio Oda

发明人： Hiroshi Tamura ,  Shigenori Tanaka ,  Jun Aketagawa ,  Toshio Oda

IPC分类号： G01N33/579 ,  C12Q1/34 ,  C12N9/99 ,  C12Q1/37

CPC分类号： G01N33/579

摘要： An endotoxin stabilizing agent is provided, which is useful in order to maintain the endotoxin activity of a specimen or a reference standard in a stable state for a prolonged period of time and to prepare an endotoxin reference standard having a reduced intervial variation, being stable for a long time in the form of, in particular, a solution and withstanding repeated use. An endotoxin composition comprising the above-mentioned endotoxin stabilizing agent and endotoxin and a method for assaying endotoxin by using the same are also provided. The endotoxin stabilizing agent comprises: (1) at least one substance selected from the group consisting of aminoalcohols, polyhydric alcohols, nonionic surfactants, polysucroses and chelating agents; (2) at least aminoalcohol and polyhydric alcohol; (3) with the polyhydric alcohol being preferably glycerol or its derivative; (4) polyethylene glycol as an effective ingredient in addition to the components (1) to (3); or (5) at least polysucrose, and an alkaline earth metal salt and/or polyethylene glycol as an effective ingredient. The endotoxin composition comprises the above-identified endotoxin stabilizing agent. The method for assaying endotoxin comprises adding the above-described endotoxin stabilizing agent to a specimen to thereby stabilize the activity of endotoxin in the specimen.

摘要翻译： 提供了一种内毒素稳定剂，其可用于将试样或参考标准品的内毒素活性长时间维持在稳定状态，并制备具有减小的间隙变化的内毒素参照标准物，其对于 很长一段时间的形式，特别是一个解决方案，并承受反复使用。 还提供了包含上述内毒素稳定剂和内毒素的内毒素组合物和使用该内毒素测定内毒素的方法。 所述内毒素稳定剂包括：（1）选自氨基醇，多元醇，非离子表面活性剂，多糖和螯合剂中的至少一种物质; （2）至少氨基醇和多元醇; （3）多元醇优选为甘油或其衍生物; （4）除了组分（1）至（3）之外的聚乙二醇作为有效成分; 或（5）至少聚蔗糖，以及碱土金属盐和/或聚乙二醇作为有效成分。 内毒素组合物包含上述内毒素稳定剂。 用于测定内毒素的方法包括向样品中加入上述内毒素稳定剂，从而稳定样品中内毒素的活性。

公开(公告)号：US5648230A

公开(公告)日：1997-07-15

申请号：US474722

申请日：1995-06-07

申请人： Hiroshi Tamura ,  Shigenori Tanaka ,  Jun Aketagawa ,  Toshio Oda

发明人： Hiroshi Tamura ,  Shigenori Tanaka ,  Jun Aketagawa ,  Toshio Oda

IPC分类号： G01N33/579 ,  C12Q1/34

CPC分类号： G01N33/579

摘要： An endotoxin stabilizing agent is provided, which is useful in order to maintain the endotoxin activity of a specimen or a reference standard in a stable state for a prolonged period of time and to prepare an endotoxin reference standard having a reduced intervial variation, being stable for a long time in the form of, in particular, a solution and withstanding repeated use. An endotoxin composition comprising the above-mentioned endotoxin stabilizing agent and endotoxin and a method for assaying endotoxin by using the same are also provided. The endotoxin stabilizing agent comprises: (1) at least one substance selected from the group consisting of aminoalcohols, polyhydric alcohols, nonionic surfactants, polysucroses and chelating agents; (2) at least aminoalcohol and polyhydric alcohol; (3) with the polyhydric alcohol being preferably glycerol or its derivative; (4) polyethylene glycol as an effective ingredient in addition to the components (1) to (3); or (5) at least polysucrose, and an alkaline earth metal salt and/or polyethylene glycol as an effective ingredient. The endotoxin composition comprises the above-identified endotoxin stabilizing agent. The method for assaying endotoxin comprises adding the above-described endotoxin stabilizing agent to a specimen to thereby stabilize the activity of endotoxin in the specimen.

摘要翻译： 提供了一种内毒素稳定剂，其可用于将试样或参考标准品的内毒素活性长时间维持在稳定状态，并制备具有减小的间隙变化的内毒素参照标准物，其对于 很长一段时间的形式，特别是一个解决方案，并承受反复使用。 还提供了包含上述内毒素稳定剂和内毒素的内毒素组合物和使用该内毒素测定内毒素的方法。 所述内毒素稳定剂包括：（1）选自氨基醇，多元醇，非离子表面活性剂，多糖和螯合剂中的至少一种物质; （2）至少氨基醇和多元醇; （3）多元醇优选为甘油或其衍生物; （4）除了组分（1）至（3）之外的聚乙二醇作为有效成分; 或（5）至少聚蔗糖，以及碱土金属盐和/或聚乙二醇作为有效成分。 内毒素组合物包含上述内毒素稳定剂。 用于测定内毒素的方法包括向样品中加入上述内毒素稳定剂，从而稳定样品中内毒素的活性。

公开(公告)号：US5389547A

公开(公告)日：1995-02-14

申请号：US58306

申请日：1993-05-10

申请人： Shigenori Tanaka ,  Hiroshi Tamura

发明人： Shigenori Tanaka ,  Hiroshi Tamura

IPC分类号： C12Q1/04 ,  C12Q1/37 ,  G01N33/531 ,  G01N33/579 ,  G01N33/00

CPC分类号： G01N33/579 ,  Y10T436/10 ,  Y10T436/14 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/25125

摘要： A reagent denatures or eliminates factors interfering with biochemical reactions by simple treatment without requiring separation of any denatured product precipitate. The reagent makes it possible to assay, in particular, .beta.-glucan and endotoxin in blood-derived samples rapidly and efficiently with high sensitivity. The reagent includes a hexadimethrine compound and an alkali metal hydroxide or an alkali metal hydroxide as a main component. A method for assaying a substance specifically reacting with a Limulus reagent utilizing the reagent, an assay kit including at least the reagent and a Limulus reagent, and a method of diagnosing infectious diseases based on the results obtained by the assay method are also provided.

摘要翻译： 试剂通过简单的处理变性或消除干扰生化反应的因素，而不需要分离任何变性产物沉淀物。 该试剂使得可以以高灵敏度快速有效地测定血液来源的样品中的β-葡聚糖和内毒素。 该试剂含有己二胺化合物和碱金属氢氧化物或碱金属氢氧化物作为主要成分。 还提供了一种使用试剂测定与鲎试剂特异性反应的物质的方法，至少包含试剂和鲎试剂的测定试剂，以及基于通过测定方法获得的结果诊断感染性疾病的方法。