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1.发明申请NOVEL TRANSAMINASE EXHIBITING HIGH ACTIVITY FOR GLUTAMIC ACID, GENE ENCODING SAME, AND METHOD FOR USING THEM 有权新型透明质酸酶展示用于谷氨酸的高活性，编码它们的基因及其使用方法公开(公告)号：US20130196389A1
公开(公告)日：2013-08-01
申请号：US13876412
申请日：2011-09-28
申请人： Noriyuki Ito , Akiko Nishi , Shigeru Kawano , Yoshihiko Yasohara
发明人： Noriyuki Ito , Akiko Nishi , Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C12N9/10
CPC分类号： C12N9/1096 , C12P17/10 , C12P41/006 , C12Y206/01 , Y02P20/52
摘要： A method for inexpensively and efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide exhibiting higher activity for glutamic acid as an amino donor than that for L-alanine, and, having novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.
摘要翻译： 提供了一种从酮化合物廉价有效地制备用作药物制剂，农药等的中间体的旋光性氨基化合物的方法。 具体而言，对于产生具有93％以上光学纯度的（S）-1-苄基-3-吡咯烷酮的新型转氨酶活性，作为氨基供体的谷氨酸比L-丙氨酸具有较高活性的多肽， 本发明还提供了编码该基因的基因，以及高水平表达该基因的转化体。
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2.发明申请MODIFIED AMINOTRANSFERASE, GENE THEREOF, AND METHOD FOR PRODUCING OPTICALLY ACTIVE AMINO COMPOUND USING SAME 有权改性氨基转移酶及其基因及其生产使用其的光学活性氨基化合物的方法公开(公告)号：US20140004575A1
公开(公告)日：2014-01-02
申请号：US14004293
申请日：2012-03-09
申请人： Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
发明人： Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C12N9/10
CPC分类号： C12N9/1096 , C12P13/001 , C12P13/04 , C12P41/006
摘要： Provided is a method for efficiently producing from a ketone compound an optically active amino compound useful as an intermediate of a drug, an agricultural chemical, or the like. Provided are: a polypeptide having aminotransferase activity that is increased in stereoselectivity, heat resistance, and resistance to amine compounds compared to the wild type enzyme by means of modifying an aminotransferase derived from Pseudomonas fluorescens; a gene encoding the polypeptide; and a transformant that expresses the gene at a high level.
摘要翻译： 提供一种从酮化合物有效地制备用作药物，农药等的中间体的光学活性氨基化合物的方法。 提供：通过修饰衍生自荧光假单胞菌的氨基转移酶，与野生型酶相比，具有增加立体选择性，耐热性和耐胺性化合物的氨基转移酶活性的多肽; 编码该多肽的基因; 以及高水平表达基因的转化体。
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3.发明授权Method for producing optically-active amine compound, recombinant vector, and transformant containing the vector 有权生产光学活性胺化合物，重组载体和含有该载体的转化体的方法公开(公告)号：US08168412B2
公开(公告)日：2012-05-01
申请号：US12302460
申请日：2007-05-28
申请人： Shigeru Kawano , Noriyuki Ito , Yoshihiko Yasohara
发明人： Shigeru Kawano , Noriyuki Ito , Yoshihiko Yasohara
IPC分类号： C12P13/00
CPC分类号： C12P13/008 , C12N9/0004 , C12N9/0006 , C12N9/1096 , C12P13/001 , C12P13/002 , C12P17/10
摘要： The present invention relates to a method for producing an optically-active amine compound. The method is characterized by using a transaminase (A), an α-keto acid reductase (B), and an enzyme (C), each having specific properties, in an identical reaction system to convert a ketone compound into a corresponding optically-active amine compound in which a carbon atom with an amino group bonded thereto serves as an asymmetric point. The present invention also relates to a recombinant vector for use in the method. The present invention makes it possible to efficiently produce an optically-active amine compound.
摘要翻译： 本发明涉及一种光学活性胺化合物的制造方法。 该方法的特征在于在相同的反应体系中使用转氨酶（A），α-酮酸还原酶（B）和具有特定性质的酶（C），以将酮化合物转化为相应的光学活性 具有与其结合的氨基的碳原子作为不对称点的胺化合物。 本发明还涉及用于该方法的重组载体。 本发明使得有效地制备光学活性胺化合物成为可能。
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4.发明授权Nucleic acid encoding a polypeptide having aminotransferase activity, vectors and host cells comprising the nucleic acid 有权编码具有氨基转移酶活性的多肽的核酸，载体和包含该核酸的宿主细胞公开(公告)号：US09481870B2
公开(公告)日：2016-11-01
申请号：US13876400
申请日：2011-09-28
申请人： Toru Nagasawa , Toyokazu Yoshida , Kouichi Ishida , Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
发明人： Toru Nagasawa , Toyokazu Yoshida , Kouichi Ishida , Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
CPC分类号： C12N9/1096 , C12P17/10 , C12P41/006 , C12Y206/01 , Y02P20/52
摘要： A method for efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide having high resistance to a water-soluble organic solvent and novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.
摘要翻译： 提供了从酮化合物有效地制备用作药物制剂，农药等的中间体的光学活性氨基化合物的方法。 具体地说，对水溶性有机溶剂具有高抗性的多肽和具有93％以上的光学纯度的（S）-1-苄基-3-吡咯烷酮的新型转氨酶活性，编码该多肽的基因，以及转化体 本文还提供了表达高水平的基因。
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5.发明申请METHOD FOR PRODUCING OPTICALLY-ACTIVE AMINE COMPOUND, RECOMBINANT VECTOR, AND TRANSFORMANT CONTAINING THE VECTOR 有权生产光活性胺化合物的方法，重组载体和包含载体的转化体公开(公告)号：US20090148899A1
公开(公告)日：2009-06-11
申请号：US12302460
申请日：2007-05-28
申请人： Shigeru Kawano , Noriyuki Ito , Yoshihiko Yasohara
发明人： Shigeru Kawano , Noriyuki Ito , Yoshihiko Yasohara
CPC分类号： C12P13/008 , C12N9/0004 , C12N9/0006 , C12N9/1096 , C12P13/001 , C12P13/002 , C12P17/10
摘要： The present invention relates to a method for producing an optically-active amine compound. The method is characterized by using a transaminase (A), an α-keto acid reductase (B), and an enzyme (C), each having specific properties, in an identical reaction system to convert a ketone compound into a corresponding optically-active amine compound in which a carbon atom with an amino group bonded thereto serves as an asymmetric point. The present invention also relates to a recombinant vector for use in the method. The present invention makes it possible to efficiently produce an optically-active amine compound.
摘要翻译： 本发明涉及一种光学活性胺化合物的制造方法。 该方法的特征在于在相同的反应体系中使用转氨酶（A），α-酮酸还原酶（B）和具有特定性质的酶（C），以将酮化合物转化为相应的光学活性 具有与其结合的氨基的碳原子作为不对称点的胺化合物。 本发明还涉及用于该方法的重组载体。 本发明使得有效地制备光学活性胺化合物成为可能。
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6.发明授权Nucleic acid encoding a polpeptide having aminotransferase activity, vectors and host cells comprising the nucleic acid 有权编码具有氨基转移酶活性的多肽的核酸，载体和包含该核酸的宿主细胞公开(公告)号：US09481871B2
公开(公告)日：2016-11-01
申请号：US13876412
申请日：2011-09-28
申请人： Noriyuki Ito , Akiko Nishi , Shigeru Kawano , Yoshihiko Yasohara
发明人： Noriyuki Ito , Akiko Nishi , Shigeru Kawano , Yoshihiko Yasohara
CPC分类号： C12N9/1096 , C12P17/10 , C12P41/006 , C12Y206/01 , Y02P20/52
摘要： A method for inexpensively and efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide exhibiting higher activity for glutamic acid as an amino donor than that for L-alanine, and, having novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.
摘要翻译： 提供了一种从酮化合物廉价有效地制备用作药物制剂，农药等的中间体的旋光性氨基化合物的方法。 具体而言，对于产生具有93％以上光学纯度的（S）-1-苄基-3-吡咯烷酮的新型转氨酶活性，作为氨基供体的谷氨酸比L-丙氨酸具有较高活性的多肽， 本发明还提供了编码该基因的基因，以及高水平表达该基因的转化体。
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7.发明授权Modified aminotransferase, gene thereof, and method for producing optically active amino compound using same 有权修饰的氨基转移酶及其基因，以及使用其制备光学活性氨基化合物的方法公开(公告)号：US09029113B2
公开(公告)日：2015-05-12
申请号：US14004293
申请日：2012-03-09
申请人： Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
发明人： Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
CPC分类号： C12N9/1096 , C12P13/001 , C12P13/04 , C12P41/006
摘要： Provided is a method for efficiently producing from a ketone compound an optically active amino compound useful as an intermediate of a drug, an agricultural chemical, or the like. Provided are: a polypeptide having aminotransferase activity that is increased in stereoselectivity, heat resistance, and resistance to amine compounds compared to the wild type enzyme by means of modifying an aminotransferase derived from Pseudomonas fluorescens; a gene encoding the polypeptide; and a transformant that expresses the gene at a high level.
摘要翻译： 提供一种从酮化合物有效地制备用作药物，农药等的中间体的光学活性氨基化合物的方法。 提供：通过修饰衍生自荧光假单胞菌的氨基转移酶，与野生型酶相比，具有增加立体选择性，耐热性和耐胺性化合物的氨基转移酶活性的多肽; 编码该多肽的基因; 以及高水平表达基因的转化体。
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公开(公告)号：US20130210092A1
公开(公告)日：2013-08-15
申请号：US13876400
申请日：2011-09-28
申请人： Toru Nagasawa , Toyokazu Yoshida , Kouichi Ishida , Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
发明人： Toru Nagasawa , Toyokazu Yoshida , Kouichi Ishida , Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C12N9/10
CPC分类号： C12N9/1096 , C12P17/10 , C12P41/006 , C12Y206/01 , Y02P20/52
摘要： A method for efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide having high resistance to a water-soluble organic solvent and novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.
摘要翻译： 提供了从酮化合物有效地制备用作药物制剂，农药等的中间体的光学活性氨基化合物的方法。 具体地说，对水溶性有机溶剂具有高抗性的多肽和具有93％以上的光学纯度的（S）-1-苄基-3-吡咯烷酮的新型转氨酶活性，编码该多肽的基因，以及转化体 本文还提供了表达高水平的基因。
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公开(公告)号：US20130183716A1
公开(公告)日：2013-07-18
申请号：US12680050
申请日：2008-09-24
申请人： Shigeru Kawano , Yoshihiko Yasohara
发明人： Shigeru Kawano , Yoshihiko Yasohara
CPC分类号： C12N9/0006 , C12Q1/006 , C12Q1/32 , C12Q1/54 , C12Y101/01047
摘要： The invention relates to a glucose dehydrogenase showing an NADP/NAD activity ratio, namely the value obtained by dividing the enzyme activity value obtained by using NADP as a coenzyme by the enzyme activity value obtained by using NAD as a coenzyme, of not lower than 300, to a gene coding therefor, and to a transformant harboring that gene. The enzyme of the invention is very high in NADP specificity and can be suitably used, for example, for NADPH production, for coenzyme regeneration in enzymatic reduction reactions, and in biosensors for glucose concentration measurements.
摘要翻译： 本发明涉及显示NADP / NAD活性比的葡萄糖脱氢酶，即通过将通过使用NADP作为辅酶获得的酶活性值除以通过使用NAD作为辅酶得到的酶活性值而得到的值为300以上 ，编码该基因的基因，以及携带该基因的转化体。 本发明的酶具有非常高的NADP特异性，并且可以适当地用于例如NADPH生产，酶促还原反应中的辅酶再生，以及生物传感器用于葡萄糖浓度测量。
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公开(公告)号：US08129163B2
公开(公告)日：2012-03-06
申请号：US12516388
申请日：2007-11-27
CPC分类号： C12P7/22 , C12N9/0006 , C12P7/04 , Y02E50/17
摘要： An object of the present invention is to provide a novel alcohol dehydrogenase, a gene for the alcohol dehydrogenase, a vector including the gene, a transformant transformed with the vector, and a method for producing an optically active alcohol by utilizing them. A feature of the present invention directs to a novel polypeptide isolated from Candida maltosa, a DNA coding for the polypeptide, and a transformant producing the polypeptide. Another feature of the present invention directs to a method for producing an optically-active alcohol by reducing a carbonyl compound with the polypeptide or the transformant.
摘要翻译： 本发明的目的是提供一种新的醇脱氢酶，用于醇脱氢酶的基因，包含该基因的载体，用载体转化的转化体，以及通过利用它们制备光学活性醇的方法。 本发明的特征指导从恶臭假丝酵母分离的新型多肽，编码多肽的DNA和产生多肽的转化体。 本发明的另一个特征指出通过用多肽或转化体还原羰基化合物来制备光学活性醇的方法。
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