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    Transgenic Bird And Method Of Constructing The Same
    3.
    发明申请
    Transgenic Bird And Method Of Constructing The Same 审中-公开
    转基因鸟及其构建方法

    公开(公告)号：US20070214511A1

    公开(公告)日：2007-09-13

    申请号：US10585693

    申请日：2004-11-05

    申请人： Takashi Yamashita ,  Takuya Shindo ,  Shinji Iijima ,  Masamichi Kamihira ,  Kenichi Nishijima

    发明人： Takashi Yamashita ,  Takuya Shindo ,  Shinji Iijima ,  Masamichi Kamihira ,  Kenichi Nishijima

    IPC分类号： A01K67/027 ,  C12N15/89 ,  C12N5/16 ,  C12P21/00 ,  C12Q1/68

    CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/30 ,  A01K2267/01

    摘要： The present invention provides a G1 transgenic bird or an offspring thereof by: incubating a fertilized avian egg, microinjecting, into the early embryo thereof at a stage except for and after the blastodermic stage just after egg laying, a replication-deficient retroviral vector coding for a useful protein, allowing the egg to hatch out to thereby obtain a G0 transgenic chimeric bird, and mating the G0 transgenic chimeric bird with another G0 transgenic chimeric bird or an offspring thereof or with a wild-type bird. The present invention is a G1 transgenic bird or an offspring thereof by: incubating a fertilized avian egg, microinjecting, into the early embryo thereof at a stage except for and after the blastodermic stage just after egg laying, a replication-deficient retroviral vector coding for a useful protein, allowing the egg to hatch out to thereby obtain a G0 transgenic chimeric bird, and mating the G0 transgenic chimeric bird with another G0 transgenic chimeric bird or an offspring thereof or with a wild-type bird.

    摘要翻译： 本发明通过以下方式提供G1转基因鸟或其后代：在产卵后的胚根期以外的阶段将受精禽卵孵育至其早期胚胎，将其编码为复制缺陷型逆转录病毒载体 一种有用的蛋白质，允许卵孵出，从而获得G0转基因嵌合鸟，并将G0转基因嵌合鸟与另一种G0转基因嵌合鸟或其后代或野生型鸟交配。 本发明是一种G1转基因鸟或其后代，其特征在于：在产卵后的胚根期以外的阶段将受精禽卵孵化至其早期胚胎，将其编码为复制缺陷型逆转录病毒载体 一种有用的蛋白质，允许卵孵出，从而获得G0转基因嵌合鸟，并将G0转基因嵌合鸟与另一种G0转基因嵌合鸟或其后代或野生型鸟交配。

    Method of constructing transgenic bird using lentivirus vector and transgenic bird obtained thereby
    4.
    发明申请
    Method of constructing transgenic bird using lentivirus vector and transgenic bird obtained thereby 审中-公开
    使用慢病毒载体和由此获得的转基因鸟构建转基因鸟的方法

    公开(公告)号：US20060259997A1

    公开(公告)日：2006-11-16

    申请号：US10569268

    申请日：2004-08-27

    申请人： Shinji Iijima ,  Masamichi Kamihira ,  Kenichi Nishijima

    发明人： Shinji Iijima ,  Masamichi Kamihira ,  Kenichi Nishijima

    IPC分类号： A01K67/027 ,  C12N5/06 ,  C12N15/867

    CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K2217/05 ,  A01K2227/30 ,  C12N15/86 ,  C12N2740/15043 ,  C12N2740/15045

    摘要： The present invention provides a lentivirus vector which comprises a coat protein containing VSV-G; a recombinant bird host which is constructed by infecting a bird host with the lentivirus vector; a method of constructing the same; a transgenic chimeric bird which is constructed by infection with the lentivirus vector and offspring thereof. The present invention also provides a method of constructing a G0 transgenic chimeric bird and offspring thereof which comprises infecting fertilized bird egg early embryos with a lentivirus vector comprising a coat protein containing VSV-G, and incubating the embryos; and a G0 transgenic chimeric bird and offspring thereof which is constructed by the method mentioned above. The present invention provides a transgenic bird which is constructed by infecting a bird early embryos with a lentivirus vector comprising a coat protein pseudotyped with VSV-G.

    摘要翻译： 本发明提供一种慢病毒载体，其包含含有VSV-G的外壳蛋白; 通过用慢病毒载体感染鸟类宿主构建的重组禽类宿主; 一种构建它的方法; 通过感染慢病毒载体及其后代构建的转基因嵌合鸟。 本发明还提供了构建G0转基因嵌合鸟及其后代的方法，其包括用含有VSV-G的外壳蛋白的慢病毒载体感染受精鸟卵早期胚胎，并培养胚胎; 和通过上述方法构建的G0转基因嵌合鸟及其后代。 本发明提供一种转基因鸟，其通过用包含用VSV-G假型包被的蛋白质的慢病毒载体感染鸟类早期胚胎而构建。

    Method for immobilizing biocatalyst in granular form with a photo-crosslinkable resin
    6.
    发明授权
    Method for immobilizing biocatalyst in granular form with a photo-crosslinkable resin 失效
    用光交联树脂固定颗粒状生物催化剂的方法

    公开(公告)号：US5268286A

    公开(公告)日：1993-12-07

    申请号：US665360

    申请日：1991-03-06

    申请人： Takeshi Kobayashi ,  Shinji Iijima ,  Masamichi Kamihira ,  Mikiko Hata ,  Takamitsu Iida ,  Masahiro Sakamoto ,  Hiroshi Itoh ,  Naoki Isobe

    发明人： Takeshi Kobayashi ,  Shinji Iijima ,  Masamichi Kamihira ,  Mikiko Hata ,  Takamitsu Iida ,  Masahiro Sakamoto ,  Hiroshi Itoh ,  Naoki Isobe

    IPC分类号： C12N11/08 ,  C12N11/10 ,  C12N11/04 ,  C12N11/12

    CPC分类号： C12N11/10 ,  C12N11/08

    摘要： A biocatalyst is immobilized with a graft product of a polymer and saponified polyvinyl acetate containing a stilbazolium group as a photo-crosslinking group. The polymer is preferably gelatin, collagen, starch, cellulose, gum arabic, tragacanth gum, carrageenan, mannan, dextrin or alginic acid. The graft product is prepared by bonding the polymer to the polyvinyl acetate, either directly through functional groups of the polymer and polyvinyl acetate or through a crosslinking agent. The polymer provides affinity for the biocatalyst and by grafting the polymer to the saponified polyvinyl acetate, damage to cells upon immobilization is reduced. A liquid composition containing a biocatalyst and the graft product is added to an aqueous solution of an inorganic salt or an organic salt to form a granular gel and the gel is irradiated with actinic rays to cure the gel.

    摘要翻译： 生物催化剂用聚合物的接枝产物和含有咔唑基作为光交联基团的皂化聚乙酸乙烯酯固定化。 聚合物优选为明胶，胶原，淀粉，纤维素，阿拉伯胶，黄蓍胶，角叉菜胶，甘露聚糖，糊精或海藻酸。 通过将聚合物直接通过聚合物和聚乙酸乙烯酯的官能团或通过交联剂直接结合聚乙酸乙烯酯来制备接枝产物。 聚合物对生物催化剂提供亲和性，并且通过将聚合物接枝到皂化的聚乙酸乙烯酯上，固定时对细胞的损伤降低。 将含有生物催化剂和接枝产物的液体组合物加入到无机盐或有机盐的水溶液中以形成颗粒状凝胶，并用光化射线照射凝胶以固化凝胶。