公开(公告)号：US09610367B2

公开(公告)日：2017-04-04

申请号：US13817711

申请日：2011-08-23

申请人： Shinzaburo Ito ,  Masato Minami ,  Tatsuki Fukui ,  Kouichi Kato ,  Fumio Yamauchi ,  Yoshinori Tomida ,  Satoshi Yuasa ,  Tetsuya Yano ,  Satoshi Ogawa ,  Kengo Kanazaki ,  Atsushi Takahashi ,  Daisuke Sasaguri ,  Hiroyuki Aoki

发明人： Shinzaburo Ito ,  Masato Minami ,  Tatsuki Fukui ,  Kouichi Kato ,  Fumio Yamauchi ,  Yoshinori Tomida ,  Satoshi Yuasa ,  Tetsuya Yano ,  Satoshi Ogawa ,  Kengo Kanazaki ,  Atsushi Takahashi ,  Daisuke Sasaguri ,  Hiroyuki Aoki

IPC分类号： A61K9/00 ,  A61K49/00 ,  A61K9/51 ,  A61K49/22 ,  B82Y15/00 ,  B82Y30/00

CPC分类号： A61K49/0093 ,  A61K9/5153 ,  A61K49/0034 ,  A61K49/0052 ,  A61K49/225 ,  B82Y15/00 ,  B82Y30/00

摘要： There is provided an ICG-loaded polymer nanoparticle that is dynamically stable, prevents the leakage of contained ICG and the resulting discoloration, and has a high molar absorbance coefficient. The particle contains a hydrophilic dye having a sulfonate group and a hydrophobic polymer, and the particle further contains at least one of a lipid having a positively charged region, a nicotinic acid derivative and a thiamine derivative.

公开(公告)号：US20130209367A1

公开(公告)日：2013-08-15

申请号：US13817711

申请日：2011-08-23

申请人： Shinzaburo Ito ,  Masato Minami ,  Tatsuki Fukui ,  Kouichi Kato ,  Fumio Yamauchi ,  Yoshinori Tomida ,  Satoshi Yuasa ,  Tetsuya Yano ,  Satoshi Ogawa ,  Kengo Kanazaki ,  Atsushi Takahashi ,  Daisuke Sasaguri ,  Hiroyuki Aoki

发明人： Shinzaburo Ito ,  Masato Minami ,  Tatsuki Fukui ,  Kouichi Kato ,  Fumio Yamauchi ,  Yoshinori Tomida ,  Satoshi Yuasa ,  Tetsuya Yano ,  Satoshi Ogawa ,  Kengo Kanazaki ,  Atsushi Takahashi ,  Daisuke Sasaguri ,  Hiroyuki Aoki

IPC分类号： A61K49/00 ,  A61K49/22

CPC分类号： A61K49/0093 ,  A61K9/5153 ,  A61K49/0034 ,  A61K49/0052 ,  A61K49/225 ,  B82Y15/00 ,  B82Y30/00

摘要： There is provided an ICG-loaded polymer nanoparticle that is dynamically stable, prevents the leakage of contained ICG and the resulting discoloration, and has a high molar absorbance coefficient. The particle contains a hydrophilic dye having a sulfonate group and a hydrophobic polymer, and the particle further contains at least one of a lipid having a positively charged region, a nicotinic acid derivative and a thiamine derivative.

摘要翻译： 提供了一种负载ICG的聚合物纳米颗粒，其动态稳定，防止所含ICG的泄漏和所得的变色，并具有高的摩尔吸光系数。 该颗粒含有具有磺酸盐基团和疏水性聚合物的亲水性染料，并且该颗粒还含有具有带正电荷区域的脂质，烟酸衍生物和硫胺衍生物中的至少一种。

公开(公告)号：US20120027679A1

公开(公告)日：2012-02-02

申请号：US13112135

申请日：2011-05-20

申请人： Fumio Yamauchi ,  Satoshi Ogawa ,  Kengo Kanazaki ,  Sachiko Inoue ,  Tatsuki Fukui ,  Masato Minami ,  Mayuko Kishi

发明人： Fumio Yamauchi ,  Satoshi Ogawa ,  Kengo Kanazaki ,  Sachiko Inoue ,  Tatsuki Fukui ,  Masato Minami ,  Mayuko Kishi

IPC分类号： A61K49/00 ,  B32B5/16 ,  C07K17/14 ,  G01N33/574 ,  B82Y15/00

CPC分类号： A61K49/221 ,  A61K41/00 ,  A61K47/6855 ,  A61K49/225 ,  A61K49/226 ,  G01N33/53 ,  Y10T428/2982 ,  Y10T428/2989

摘要： It is intended to provide a novel contrast agent for photoacoustic imaging that is highly capable of binding to a target molecule and generates high photoacoustic signals. The present invention provides a contrast agent for photoacoustic imaging represented by Formula 1: MNP−((L)l−(P)m)n   (Formula 1) (wherein MNP represents a particle containing an iron oxide particle; L represents a linker molecule; P represents a ligand molecule; l represents 0 or 1; and m and n represent an integer of 1 or larger), the contrast agent for photoacoustic imaging including: a particle containing an iron oxide particle that absorbs light in a near-infrared region; and at least one or more ligand molecule(s) immobilized on the particle containing an iron oxide particle, wherein the immobilization density of the ligand molecule is equal to or higher than the cell surface density of a target molecule.

摘要翻译： 旨在提供一种新颖的光声成像造影剂，其具有高度能够结合靶分子并产生高光声信号。 本发明提供了由式1表示的光声成像造影剂：MNP - （（L）1-（P）m）n（式1）（其中，MNP表示含有氧化铁颗粒的颗粒; L表示连接分子 ; P表示配体分子; l表示0或1; m和n表示1或更大的整数），光声成像用造影剂包括：含有吸收近红外区域的光的氧化铁粒子的粒子 ; 和固定在含有氧化铁颗粒的颗粒上的至少一种或多种配体分子，其中配体分子的固定密度等于或高于靶分子的细胞表面密度。

公开(公告)号：US20110117023A1

公开(公告)日：2011-05-19

申请号：US13014345

申请日：2011-01-26

申请人： Fumio Yamauchi ,  Satoshi Ogawa ,  Kengo Kanazaki ,  Sachiko Inoue ,  Tatsuki Fukui ,  Masato Minami ,  Mayuko Kishi

发明人： Fumio Yamauchi ,  Satoshi Ogawa ,  Kengo Kanazaki ,  Sachiko Inoue ,  Tatsuki Fukui ,  Masato Minami ,  Mayuko Kishi

IPC分类号： A61K49/00 ,  C07K16/00 ,  C07K2/00 ,  G01N33/53 ,  C12Q1/02

CPC分类号： A61K41/00 ,  A61K47/6855 ,  A61K49/225 ,  A61K49/226 ,  Y10T428/2982

摘要： It is intended to provide a novel contrast agent for photoacoustic imaging that is highly capable of binding to a target molecule and generates high photoacoustic signals. The present invention provides a contrast agent for photoacoustic imaging represented by Formula 1: MNP-((L)l-(P)m)n  (Formula 1) (wherein MNP represents a particle containing an iron oxide particle; L represents a linker molecule; P represents a ligand molecule; l represents 0 or 1; and m and n represent an integer of 1 or larger), the contrast agent for photoacoustic imaging including: a particle containing an iron oxide particle that absorbs light in a near-infrared region; and at least one or more ligand molecule(s) immobilized on the particle containing an iron oxide particle, wherein the immobilization density of the ligand molecule is equal to or higher than the cell surface density of a target molecule.

摘要翻译： 旨在提供一种新颖的光声成像造影剂，其具有高度能够结合靶分子并产生高光声信号。 本发明提供了由式1表示的光声成像造影剂：MNP - （（L）1-（P）m）n（式1）（其中MNP表示含有氧化铁颗粒的颗粒; L表示连接分子 ; P表示配体分子; l表示0或1; m和n表示1或更大的整数），光声成像用造影剂包括：含有吸收近红外区域的光的氧化铁粒子的粒子 ; 和固定在含有氧化铁颗粒的颗粒上的至少一种或多种配体分子，其中配体分子的固定密度等于或高于靶分子的细胞表面密度。

公开(公告)号：US20120165646A1

公开(公告)日：2012-06-28

申请号：US13328802

申请日：2011-12-16

申请人： Fumio Yamauchi ,  Satoshi Ogawa ,  Kengo Kanazaki ,  Tatsuki Fukui ,  Sachiko Inoue

发明人： Fumio Yamauchi ,  Satoshi Ogawa ,  Kengo Kanazaki ,  Tatsuki Fukui ,  Sachiko Inoue

IPC分类号： A61B5/05 ,  A61K49/00 ,  G01N33/53 ,  C07K16/00

CPC分类号： A61K49/225 ,  A61B5/0095 ,  A61B8/481

摘要： Regarding a composite particle in the related art, an antibody and a dye are bound to the surface of the particle. Therefore, it is not possible to bind both the antibody and the dye high in number. Accordingly, the present invention provides a composite particle, wherein a large acoustic wave can be emitted and both the antibody and the dye can be bound high in number. A composite particle including a core particle, a dye bound to the above described core particle, and an antibody, wherein the above described antibody is bound to the above described dye, is suitable for use.

摘要翻译： 关于现有技术的复合粒子，抗体和染料与粒子的表面结合。 因此，不能将抗体和数量高的染料结合。 因此，本发明提供一种复合颗粒，其中可以发射大的声波，并且抗体和染料都可以结合数量高。 包含核心颗粒，与上述核心颗粒结合的染料和其中上述抗体与上述染料结合的抗体的复合颗粒适合使用。

公开(公告)号：US08491908B2

公开(公告)日：2013-07-23

申请号：US13115276

申请日：2011-05-25

申请人： Kengo Kanazaki ,  Tsutomu Honma ,  Fumio Yamauchi ,  Satoshi Ogawa ,  Sachiko Inoue

发明人： Kengo Kanazaki ,  Tsutomu Honma ,  Fumio Yamauchi ,  Satoshi Ogawa ,  Sachiko Inoue

IPC分类号： A61K39/44

CPC分类号： C07K17/08 ,  C07K17/14 ,  G01N33/533 ,  G01N33/54313

摘要： The present invention provides a composite particle having a high molar absorption coefficient for detection with higher detection sensitivity in photoacoustic imaging. In the present invention, a composite particle having a particle, a single-chain antibody which includes an antigen recognition region and a region other than the antigen recognition region and which is conjugated with the particle, and an organic dye conjugated with the single-chain antibody, in which the region other than the antigen recognition region of the single-chain antibody has thiol group, and a functional group of the particle is bound to the thiol group, is provided.

摘要翻译： 本发明提供一种具有高摩尔吸光系数的复合颗粒，用于检测光电成像中较高的检测灵敏度。 在本发明中，具有包含抗原识别区域和抗原识别区域以外的区域的粒子，单链抗体与该颗粒结合的复合粒子和与单链结合的有机染料 抗体，其中除了单链抗体的抗原识别区域以外的区域具有硫醇基，并且该颗粒的官能团与硫醇基团结合。

公开(公告)号：US08993715B2

公开(公告)日：2015-03-31

申请号：US12830244

申请日：2010-07-02

申请人： Fumio Yamauchi ,  Satoshi Ogawa ,  Kengo Kanazaki

发明人： Fumio Yamauchi ,  Satoshi Ogawa ,  Kengo Kanazaki

IPC分类号： C07K7/00 ,  C07K1/13 ,  C07K16/32

CPC分类号： C07K1/13 ,  C07K16/32 ,  C07K2317/622 ,  C07K2319/21 ,  C07K2319/31

摘要： It is an object of the present invention to obtain a labeled protein, and specifically, to separate a labeled protein and the same unlabeled protein. There is provided a labeled protein including: a protein to be labeled having a target protein, at least one or more affinity interaction domains for binding to an affinity support, and at least one or more labeling sites; and a labeling reagent binding to at least one of the labeling sites; wherein the affinity of the labeled protein for the affinity support is difference from that of the protein to be labeled for the affinity support.

摘要翻译： 本发明的目的是获得标记的蛋白质，特别是分离标记的蛋白质和相同的未标记的蛋白质。 提供了标记的蛋白质，其包括：待标记的蛋白质，其具有靶蛋白质，至少一个或多个与亲和载体结合的亲和作用结构域，以及至少一个或多个标记位点; 和与至少一个标记位点结合的标记试剂; 其中所述标记的蛋白质对所述亲和载体的亲和力与所述亲和载体的待标记蛋白质的亲和力不同。

公开(公告)号：US08450462B2

公开(公告)日：2013-05-28

申请号：US12821711

申请日：2010-06-23

申请人： Satoshi Ogawa ,  Fumio Yamauchi ,  Kengo Kanazaki ,  Mayuko Kishi

发明人： Satoshi Ogawa ,  Fumio Yamauchi ,  Kengo Kanazaki ,  Mayuko Kishi

IPC分类号： C07K16/28 ,  C07K16/46 ,  A61K49/06 ,  A61K49/16 ,  A61K49/22

CPC分类号： A61K49/221 ,  A61K49/0058 ,  A61K49/16 ,  C07K16/32 ,  C07K2317/622 ,  C07K2319/33 ,  C07K2319/50

摘要： A compound including a polymer is represented by general formula (1): L-Y-A  (1) wherein, A is a single-chain antibody moiety, which is a polypeptide including an antigen-binding site, L is a linker moiety, which is a polypeptide including a protease cleavage site, Y is a peptide moiety, which includes 0 or more amino acids connecting the linker moiety L with the single-chain antibody moiety A, and the linker moiety L binds to an N terminus of the peptide moiety Y or an N terminus of the single-chain antibody moiety A.

摘要翻译： 包括聚合物的化合物由通式（1）表示：LYA（1）其中，A是单链抗体部分，其是包含抗原结合位点的多肽，L是连接部分，其是多肽 包括蛋白酶切割位点，Y是肽部分，其包括连接接头部分L与单链抗体部分A的0个或更多个氨基酸，并且连接体部分L结合肽部分Y的N末端或 单链抗体部分A的N末端

公开(公告)号：US20110009601A1

公开(公告)日：2011-01-13

申请号：US12830244

申请日：2010-07-02

申请人： Fumio Yamauchi ,  Satoshi Ogawa ,  Kengo Kanazaki

发明人： Fumio Yamauchi ,  Satoshi Ogawa ,  Kengo Kanazaki

IPC分类号： C07K16/00 ,  C07K1/13 ,  C07K14/00

CPC分类号： C07K1/13 ,  C07K16/32 ,  C07K2317/622 ,  C07K2319/21 ,  C07K2319/31

摘要： It is an object of the present invention to obtain a labeled protein, and specifically, to separate a labeled protein and the same unlabeled protein. There is provided a labeled protein including: a protein to be labeled having a target protein, at least one or more affinity interaction domains for binding to an affinity support, and at least one or more labeling sites; and a labeling reagent binding to at least one of the labeling sites; wherein the affinity of the labeled protein for the affinity support is difference from that of the protein to be labeled for the affinity support.

摘要翻译： 本发明的目的是获得标记的蛋白质，特别是分离标记的蛋白质和相同的未标记的蛋白质。 提供了标记的蛋白质，其包括：待标记的蛋白质，其具有靶蛋白质，至少一个或多个与亲和载体结合的亲和作用结构域，以及至少一个或多个标记位点; 和与至少一个标记位点结合的标记试剂; 其中所述标记的蛋白质对所述亲和载体的亲和力与所述亲和载体的待标记蛋白质的亲和力不同。

公开(公告)号：US20110064666A1

公开(公告)日：2011-03-17

申请号：US12821711

申请日：2010-06-23

申请人： Satoshi Ogawa ,  Fumio Yamauchi ,  Kengo Kanazaki ,  Mayuko Kishi

发明人： Satoshi Ogawa ,  Fumio Yamauchi ,  Kengo Kanazaki ,  Mayuko Kishi

IPC分类号： A61K49/00 ,  C07K16/00 ,  G01N33/53 ,  C12P21/00 ,  A61P43/00

CPC分类号： A61K49/221 ,  A61K49/0058 ,  A61K49/16 ,  C07K16/32 ,  C07K2317/622 ,  C07K2319/33 ,  C07K2319/50

摘要： When an antigen present at lesion sites is detected using a compound containing a single-chain antibody moiety, the single-chain antibody moiety binds to the antigen present at sites other than lesion sites. As a result, the detection of the lesion sites with high sensitivity and high contrast is difficult, and a compound solving this problem is demanded.The present invention provides a compound including a polymer represented by general formula (1): L-Y-A  (1) wherein, A is a single-chain antibody moiety, which is a polypeptide including an antigen-binding site; L is a linker moiety, which is a polypeptide including a protease cleavage site; Y is a peptide moiety, which including 0 or more amino acids connecting the linker moiety L with the single-chain antibody moiety A; and the linker moiety L binds to an N terminus of the peptide moiety Y or an N terminus of the single-chain antibody moeity A.

摘要翻译： 当使用含有单链抗体部分的化合物检测存在于病变部位的抗原时，单链抗体部分与存在于除病变部位之外的位点处的抗原结合。 结果，难以检测具有高灵敏度和高对比度的病变部位，需要解决这个问题的化合物。 本发明提供包含通式（1）表示的聚合物的化合物：L-Y-A（1）其中A是单链抗体部分，其是包含抗原结合位点的多肽; L是连接体部分，其是包含蛋白酶切割位点的多肽; Y是肽部分，其包括连接部分L与单链抗体部分A的0个或更多个氨基酸; 并且连接体部分L结合于肽部分Y的N末端或单链抗体动力学A的N末端。