中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

11 records (took 0.016 seconds)

    Complex cancer cells luminescence detection method
    1.
    发明申请
    Complex cancer cells luminescence detection method 审中-公开
    复合癌细胞发光检测方法

    公开(公告)号：US20080050729A1

    公开(公告)日：2008-02-28

    申请号：US11585974

    申请日：2006-10-25

    Applicant: Shiu-Ru Lin ,  Tian-Lu Cheng

    Inventor： Shiu-Ru Lin ,  Tian-Lu Cheng

    IPC: C12Q1/68

    CPC classification number: C12Q1/6837

    Abstract: A method for detecting cancer cells. A chemical luminescence reaction is used to obtain a result image. The result image is analyzed through a weighting process to a value of each gene. By doing do, a cancer can be precisely diagnosed.

    Abstract translation: 一种检测癌细胞的方法。 使用化学发光反应来获得结果图像。 通过加权过程对每个基因的值分析结果图像。 通过这样做，可以精确诊断癌症。

    Method for biochip detecting limited cells
    2.
    发明申请
    Method for biochip detecting limited cells 审中-公开
    生物芯片检测有限细胞的方法

    公开(公告)号：US20060068396A1

    公开(公告)日：2006-03-30

    申请号：US10948224

    申请日：2004-09-24

    Applicant: Shiu-Ru Lin ,  Tian-Lu Cheng ,  Yi-Fang Chen ,  Chan-Han Wu

    Inventor： Shiu-Ru Lin ,  Tian-Lu Cheng ,  Yi-Fang Chen ,  Chan-Han Wu

    IPC: C12Q1/68

    CPC classification number: C12Q1/6837 ,  C12Q2565/507

    Abstract: The present invention is a method for a biochip detecting limited cells. The present invention comprises steps of: obtaining a nylon membrane chip having required nucleotide fragments arranged in a dot matrix way by using a manual spotter; naturally drying by heat and fixing the nucleotide fragments on the nylon membrane chip by a rapid nucleic acid cross-linker when preparing a chip; collecting some normal whole blood to be linearly amplified; synthesizing required amount of cDNA by a reverse transcription to obtain a marker as a probe; processing labeling, hybridization and post-hybridization to the chip and the marker; processing chemical color reaction; and automatically analyzing the result image after the chemical color reaction. Accordingly, a gene biochip operation technology platform with low cost, easy operation and high efficiency is obtained. And so the functions and the applications of the gene biochip can be effectively worked out and the practical applications of the gene biochip on related fields can be conclusively popularized.

    Abstract translation: 本发明是一种检测有限细胞的生物芯片的方法。 本发明包括以下步骤：通过使用手动打斑机获得具有以点阵方式布置的所需核苷酸片段的尼龙膜芯片; 通过加热自然干燥，并在制备芯片时通过快速核酸交联剂固定尼龙膜芯片上的核苷酸片段; 收集一些正常全血要线性扩增; 通过逆转录合成所需量的cDNA以获得作为探针的标记; 加工标记，杂交和后期杂交到芯片和标记物; 加工化学色素反应; 并在化学反应后自动分析结果图像。 因此，获得了低成本，易于操作和高效率的基因生物芯片操作技术平台。 因此可以有效地制定基因生物芯片的功能和应用，基因生物芯片在相关领域的实际应用可以得到最终推广。

    Genes for diagnosing colorectal cancer
    5.
    发明申请
    Genes for diagnosing colorectal cancer 有权
    诊断结肠直肠癌的基因

    公开(公告)号：US20050191634A1

    公开(公告)日：2005-09-01

    申请号：US10786148

    申请日：2004-02-26

    Applicant: Shiu-Ru Lin ,  Jaw-Yuan Wang

    Inventor： Shiu-Ru Lin ,  Jaw-Yuan Wang

    IPC: C07H21/04 ,  C12P19/34 ,  C12Q1/68

    CPC classification number: C12Q1/6886 ,  C12Q2600/158

    Abstract: This invention relates to provide the genes for diagnosing colorectal cancer, the gene sequences searching comprise the steps of: (1) deriving epithelium cells from normal intestines, polypus of intestines and colorectal cancer tissue; (2) collecting genes with highly differential gene expression by Suppression Subtractive Hybridization (SSH), and building library; (3) deriving colonies with relatively high signal intensities from cancer tissue; (4) collecting more clinically cancer tissues by Northern Hybridization, real-time Polymerase Chain Reaction (PCR) combined with analysis of bioinformation to affirm variation between differential gene expression; and (5) selecting the most suitable genes from said library, and using the gene sequence as reagent provides the effects of early diagnosis, specificity, highly sensitivity and safety.

    Abstract translation: 本发明涉及提供用于诊断结肠直肠癌的基因，所述基因序列搜索包括以下步骤：（1）从正常肠道，肠道息肉和结肠直肠癌组织衍生上皮细胞; （2）通过抑制消减杂交（SSH）和建筑图书馆收集具有高度差异基因表达的基因; （3）从癌组织衍生具有较高信号强度的菌落; （4）通过Northern杂交，实时聚合酶链反应（PCR）结合生物信息分析，收集更多的临床癌症组织，证实差异基因表达之间的差异; 和（5）从所述文库中选择最合适的基因，并且使用基因序列作为试剂提供早期诊断，特异性，高灵敏度和安全性的作用。

    Method of Diagnosing Active Mycobacterium Tuberculosis with Detecting Chip
    6.
    发明申请
    Method of Diagnosing Active Mycobacterium Tuberculosis with Detecting Chip 审中-公开
    用检测芯片诊断活动性结核分枝杆菌的方法

    公开(公告)号：US20110177961A1

    公开(公告)日：2011-07-21

    申请号：US12688120

    申请日：2010-01-15

    Applicant: Shiu-Ru Lin ,  Ching-Sheng Yeh ,  Chia-Sui Sun ,  Jong-Rung Tsai ,  Ming-Je Yang

    Inventor： Shiu-Ru Lin ,  Ching-Sheng Yeh ,  Chia-Sui Sun ,  Jong-Rung Tsai ,  Ming-Je Yang

    IPC: C40B30/04

    CPC classification number: C12Q1/689 ,  C12Q1/6837

    Abstract: The present disclosure diagnoses mycobacterium tuberculosis, not mycobacterium tuberculosis complex only. Specific target genes are selected from regions of difference of mycobacterium tuberculosis. After hybridization with labeling substances, sputum samples are processed through color developments separately for obtaining images to be analyzed automatically. Thus, the present disclosure rapidly diagnoses mycobacterium tuberculosis in the sputum samples with a simple operation and a low cost.

    Abstract translation: 本公开仅诊断结核分枝杆菌，而不是结核分枝杆菌复合物。 特异性靶基因选自结核分枝杆菌差异的区域。 与标记物质杂交后，分别通过颜色开发处理痰样品，以获得自动分析的图像。 因此，本公开以简单的操作和低成本快速诊断痰样品中的结核分枝杆菌。

    Genes for diagnosing colorectal cancer
    7.
    发明授权
    Genes for diagnosing colorectal cancer 有权
    诊断结肠直肠癌的基因

    公开(公告)号：US07575928B2

    公开(公告)日：2009-08-18

    申请号：US10786148

    申请日：2004-02-26

    Applicant: Shiu-Ru Lin ,  Jaw-Yuan Wang

    Inventor： Shiu-Ru Lin ,  Jaw-Yuan Wang

    IPC: G01N33/48 ,  C12Q1/68 ,  A61B5/055 ,  C12N15/867

    CPC classification number: C12Q1/6886 ,  C12Q2600/158

    Abstract: This invention relates to provide the genes for diagnosing colorectal cancer, the gene sequences searching comprise the steps of: (1) deriving epithelium cells from normal intestines, polypus of intestines and colorectal cancer tissue; (2) collecting genes with highly differential gene expression by Suppression Subtractive Hybridization (SSH), and building library; (3) deriving colonies with relatively high signal intensities from cancer tissue; (4) collecting more clinically cancer tissues by Northern Hybridization, real-time Polymerase Chain Reaction (PCR) combined with analysis of bioinformation to affirm variation between differential gene expression; and (5) selecting the most suitable genes from said library, and using the gene sequence as reagent provides the effects of early diagnosis, specificity, highly sensitivity and safety.

    Abstract translation: 本发明涉及提供用于诊断结肠直肠癌的基因，所述基因序列搜索包括以下步骤：（1）从正常肠道，肠道息肉和结肠直肠癌组织衍生上皮细胞; （2）通过抑制消减杂交（SSH）和建筑图书馆收集具有高度差异基因表达的基因; （3）从癌组织衍生具有较高信号强度的菌落; （4）通过Northern杂交，实时聚合酶链反应（PCR）结合生物信息分析，收集更多的临床癌症组织，证实差异基因表达之间的差异; 和（5）从所述文库中选择最合适的基因，并且使用基因序列作为试剂提供早期诊断，特异性，高灵敏度和安全性的作用。

    Automatic system of isolating and incubating circulating tumor cells
    9.
    发明授权
    Automatic system of isolating and incubating circulating tumor cells 有权
    自动分离和培养循环肿瘤细胞系统

    公开(公告)号：US08309344B2

    公开(公告)日：2012-11-13

    申请号：US12578522

    申请日：2009-10-13

    Applicant: Hui-Jen Chang ,  Shiu-Ru Lin ,  Chun-Hung Lee ,  Der-And Tsao

    Inventor： Hui-Jen Chang ,  Shiu-Ru Lin ,  Chun-Hung Lee ,  Der-And Tsao

    IPC: C12M3/00 ,  C12M1/36

    CPC classification number: C12M47/04 ,  C12M41/00 ,  C12M41/34 ,  C12M41/46

    Abstract: The present invention is applied in fields like biological medicine and tissue engineering. A fluid control system in a cell isolation and culture system is used to automatically process sample preparation, circulating tumor cell (CTC) isolation, plate changing and cell culturing. By using the present invention, time and labor are saved; moreover, the present invention has a small size and is easily carried.

    Abstract translation: 本发明应用于生物医学和组织工程领域。 细胞分离培养系统中的流体控制系统用于自动处理样品制备，循环肿瘤细胞（CTC）分离，平板变化和细胞培养。 通过使用本发明，省时省力; 此外，本发明具有小尺寸并且容易携带。

    Automatic System of Isolating and Incubating Circulating Tumor Cells
    10.
    发明申请
    Automatic System of Isolating and Incubating Circulating Tumor Cells 有权
    分离和孵育循环肿瘤细胞的自动系统

    公开(公告)号：US20110059519A1

    公开(公告)日：2011-03-10

    申请号：US12578522

    申请日：2009-10-13

    Applicant: Hui-Jen Chang ,  Shiu-Ru Lin ,  Chun-Hung Lee ,  Der-An Tsao

    Inventor： Hui-Jen Chang ,  Shiu-Ru Lin ,  Chun-Hung Lee ,  Der-An Tsao

    IPC: C12M1/36

    CPC classification number: C12M47/04 ,  C12M41/00 ,  C12M41/34 ,  C12M41/46

    Abstract: The present invention is apllied in fields like biological medicine and tissue engineering. A fluid control system in a cell isolation and culture system is used to automatically process sample preparation, circulating tumor cell (CTC) isolation, plate changing and cell culturing. By using the present invention, time and labor are saved; moreover, the present invention has a small size and is easily carried.

    Abstract translation: 本发明涉及生物医学和组织工程等领域。 细胞分离培养系统中的流体控制系统用于自动处理样品制备，循环肿瘤细胞（CTC）分离，平板变化和细胞培养。 通过使用本发明，省时省力; 此外，本发明具有小尺寸并且容易携带。

Patent Agency Ranking