公开(公告)号：US20060001867A1

公开(公告)日：2006-01-05

申请号：US10879632

申请日：2004-06-30

申请人： John Maier ,  Jason Neiss ,  Shona Stewart ,  Matthew Nelson ,  Joseph Demuth ,  Patrick Treado

发明人： John Maier ,  Jason Neiss ,  Shona Stewart ,  Matthew Nelson ,  Joseph Demuth ,  Patrick Treado

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01N21/65 ,  G01J3/0297 ,  G01J3/44 ,  G01N2021/656

摘要： The embodiments disclosed herein generally relate to identifying and removing background noise in spectroscopic imaging of a sample. Because white-light has essentially constant intensity at every wavelength, background noise caused by white light can be identified and removed from spectroscopic measurements including Raman spectroscopy. Thus, once the Raman spectrum for a sample is obtained, it may be corrected to remove the white-light dispersive spectrum in accordance with the embodiments disclosed herein.

摘要翻译： 本文公开的实施例通常涉及在样本的光谱成像中识别和去除背景噪声。 因为白光在每个波长处具有基本恒定的强度，所以可以从包括拉曼光谱的光谱测量中识别和去除由白光引起的背景噪声。 因此，一旦获得样品的拉曼光谱，就可以根据本文公开的实施方案来校正其去除白光色散谱。

公开(公告)号：US20060001868A1

公开(公告)日：2006-01-05

申请号：US10879633

申请日：2004-06-30

申请人： Shona Stewart ,  Matthew Nelson ,  Joseph Demuth ,  Patrick Treado

发明人： Shona Stewart ,  Matthew Nelson ,  Joseph Demuth ,  Patrick Treado

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01J3/44 ,  G01J3/0297 ,  G01N21/65

摘要： The disclosure relates to a substrate material for the improved detection, resolution and imaging of biological material for spectroscopic characterization by Raman of optical imaging spectroscopy. The substrate provides a uniform, optically flat, highly reflective surface which can be made hydrophobic to prevent spreading of the sample and facilitating its optical evaluation. Moreover, the substrate can be coated with a material that does not emit Raman scattered photons when exposed to said illuminating photons. The principles disclosed herein allow a low spectroscopic background particularly suitable for examining small samples or samples having low concentrations of the suspected component.

摘要翻译： 本公开涉及用于通过光学成像光谱法的拉曼进行光谱表征的生物材料的改进的检测，分辨和成像的基底材料。 衬底提供均匀的光学平坦的高反射表面，其可以被制成疏水性以防止样品的扩散并且便于其光学评估。 此外，当暴露于所述照射光子时，衬底可以涂覆有不发射拉曼散射光子的材料。 本文公开的原理允许低光谱背景特别适合于检查具有低浓度可疑成分的小样品或样品。

公开(公告)号：US20070133984A1

公开(公告)日：2007-06-14

申请号：US11585535

申请日：2006-10-24

申请人： John Maier ,  Patrick Treado ,  Matthew Nelson ,  Lindy McClelland ,  Shona Stewart

发明人： John Maier ,  Patrick Treado ,  Matthew Nelson ,  Lindy McClelland ,  Shona Stewart

IPC分类号： H04B10/08 ,  H04B17/00

CPC分类号： G01N21/65 ,  G01J3/28 ,  G01J3/2823 ,  G01J3/44 ,  G01N21/274 ,  G01N2021/656

摘要： A system and method to automatically obtain spectra for samples. The method involves a two phase process including a photobleaching phase and a spectral acquisition phase. In the photobleaching phase, a series of spectral data sets of a sample are collected. A relative difference is determined between the background of subsequent spectral data sets is determined and compared to a predetermined threshold value. If threshold difference is less than the relative difference between the background of subsequent spectral data sets, the steps of collecting a series of spectra data sets is automatically repeated. In the spectrum acquisition phase, a series of Raman data sets of the sample are collected until a target SNR is obtained.

摘要翻译： 自动获取样品光谱的系统和方法。 该方法涉及包括光漂白相和光谱采集阶段的两相过程。 在漂白漂白阶段，收集样品的一系列光谱数据集。 在确定后续光谱数据集的背景并将其与预定阈值进行比较之间确定相对差。 如果阈值差小于随后的光谱数据集的背景之间的相对差，则自动重复收集一系列光谱数据集的步骤。 在频谱采集阶段，收集样本的一系列拉曼数据集，直到获得目标SNR。

公开(公告)号：US07557915B2

公开(公告)日：2009-07-07

申请号：US11585535

申请日：2006-10-24

申请人： John Maier ,  Patrick Treado ,  Matthew Nelson ,  Lindy McClelland ,  Shona Stewart

发明人： John Maier ,  Patrick Treado ,  Matthew Nelson ,  Lindy McClelland ,  Shona Stewart

IPC分类号： G01J3/44

CPC分类号： G01N21/65 ,  G01J3/28 ,  G01J3/2823 ,  G01J3/44 ,  G01N21/274 ,  G01N2021/656

摘要： A system and method to automatically obtain spectra for samples. The method involves a two phase process including a photobleaching phase and a spectral acquisition phase. In the photobleaching phase, a series of spectral data sets of a sample are collected. A relative difference is determined between the background of subsequent spectral data sets is determined and compared to a predetermined threshold value. If threshold difference is less than the relative difference between the background of subsequent spectral data sets, the steps of collecting a series of spectra data sets is automatically repeated. In the spectrum acquisition phase, a series of Raman data sets of the sample are collected until a target SNR is obtained.

摘要翻译： 自动获取样品光谱的系统和方法。 该方法涉及包括光漂白相和光谱采集阶段的两相过程。 在漂白漂白阶段，收集样品的一系列光谱数据集。 在确定后续光谱数据集的背景并将其与预定阈值进行比较之间确定相对差。 如果阈值差小于随后的光谱数据集的背景之间的相对差，则自动重复收集一系列光谱数据集的步骤。 在频谱采集阶段，收集样本的一系列拉曼数据集，直到获得目标SNR。

公开(公告)号：US08395769B2

公开(公告)日：2013-03-12

申请号：US12834462

申请日：2010-07-12

申请人： Shona Stewart ,  John Maier ,  Patrick Treado

发明人： Shona Stewart ,  John Maier ,  Patrick Treado

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01N21/65 ,  G01J3/2823 ,  G01J3/44 ,  G01N21/658 ,  G01N33/1826

摘要： A method for assessing the presence of a pathogenic microorganism in a sample. A sample is illuminated to thereby produce a first plurality of interacted photons which may be scattered, emitted, reflected and/or absorbed by the sample. The first plurality of interacted photons are assessed to thereby generate a Raman data set representative of the sample. This Raman data set is analyzed to thereby determine at least one of: the presence of a pathogenic microorganism in said sample and the absence of a pathogenic microorganism in said sample. The Raman data set may comprise at least one of a Raman spectrum and/or a Raman chemical image representative of the sample. The analysis may comprise comparing said Raman data set to at least one reference Raman data set representative of a known sample. This may be achieved using a chemometric technique.

摘要翻译： 用于评估样品中病原微生物的存在的方法。 照射样品从而产生可被样品散射，发射，反射和/或吸收的第一多个相互作用的光子。 评估第一组多个相互作用的光子，从而产生代表样品的拉曼数据集。 分析该拉曼数据集，从而确定所述样品中病原微生物的存在和所述样品中不存在致病微生物中的至少一种。 拉曼数据集可以包括代表样品的拉曼光谱和/或拉曼化学图像中的至少一个。 分析可以包括将所述拉曼数据集与代表已知样本的至少一个参考拉曼数据集进行比较。 这可以使用化学计量技术来实现。

公开(公告)号：US20120200850A1

公开(公告)日：2012-08-09

申请号：US13098832

申请日：2011-05-02

申请人： Shona Stewart ,  John Maier ,  Patrick Treado

发明人： Shona Stewart ,  John Maier ,  Patrick Treado

IPC分类号： G01J3/44

CPC分类号： G01N21/65 ,  G01N2021/656

摘要： Raman molecular imaging (RMI) is used to detect mammalian cells of a particular phenotype. The disclosure includes the use of RMI to detect transplanted and/or grafted cells, to differentiate between normal and diseased cells or tissues, as well as in determining the grade of said cancer cells. Raman scattering data may be analyzed to determine the transplant efficiency, disease state, clinical outcome, and/or prognosis of cells or tissue. This data may be combined with visual image data to produce hybrid images which depict both a magnified view of the cellular structures and information relating to the disease state of the individual cells in the field of view. Also, RMI techniques may be combined with visual image data and validated with other detection methods to produce confirm the matter obtained by RMI.

摘要翻译： 拉曼分子成像（RMI）用于检测特定表型的哺乳动物细胞。 本公开内容包括使用RMI来检测移植和/或接种的细胞，以区分正常和患病的细胞或组织，以及确定所述癌细胞的等级。 可以分析拉曼散射数据以确定细胞或组织的移植效率，疾病状态，临床结果和/或预后。 该数据可以与视觉图像数据组合以产生描绘细胞结构的放大视图和与视野中的各个细胞的疾病状态有关的信息的混合图像。 此外，RMI技术可以与可视图像数据组合，并用其他检测方法验证，以确定RMI获得的事项。

公开(公告)号：US20150294076A1

公开(公告)日：2015-10-15

申请号：US14440987

申请日：2013-11-06

申请人： Patrick TREADO ,  Shona STEWART ,  Heather KIRSCHNER ,  Ryan PRIORE ,  Alan WILSON ,  CHEMIMAGE CORPORATION

发明人： Patrick Treado ,  Shona Stewart ,  Heather Kirschner ,  Ryan Priore ,  Alan Wilson

IPC分类号： G06F19/00 ,  G06F19/24 ,  G01N21/65 ,  G01N33/49

CPC分类号： G16H50/20 ,  G01J3/1804 ,  G01J3/44 ,  G01J3/4406 ,  G01N21/65 ,  G01N33/49 ,  G01N33/57419 ,  G16B40/00

摘要： A system and method for analyzing biological samples, such as dried human blood serum, to determine a disease state such as colorectal cancer (CRC). Using dried samples may hold potential for enhancing localized concentration and/or segmentation of sample components. The method may comprise illuminating at least one location of a biological sample to generate a plurality of interacted photons, collecting the interacted photons and generating at least one Raman data set representative of the biological sample. A system may comprise an illumination source to illuminate at least one location of a biological sample and generate at least one plurality of interacted photons, at least one mirror for directing the interacted photons to a detector. The detector may be configured to generate at least one Raman data set representative of the biological sample. The system and method may utilize a FAST device for multipoint analysis or may be configured to analyze a sample using a line scanning configuration.

摘要翻译： 用于分析诸如干人血清的生物样品以确定诸如结肠直肠癌（CRC）的疾病状态的系统和方法。 使用干燥的样品可能具有增强样品组分的局部浓度和/或分割的潜力。 该方法可以包括照射生物样品的至少一个位置以产生多个相互作用的光子，收集相互作用的光子并产生代表生物样品的至少一个拉曼数据集。 系统可以包括照明源以照亮生物样品的至少一个位置并且生成至少一个多个相互作用的光子，至少一个反射镜，用于将相互作用的光子引导到检测器。 检测器可以被配置为产生代表生物样品的至少一个拉曼数据集。 该系统和方法可以利用FAST设备进行多点分析，或者可以配置为使用线扫描配置分析样本。

公开(公告)号：US20110007309A1

公开(公告)日：2011-01-13

申请号：US12834462

申请日：2010-07-12

申请人： Shona Stewart ,  John Maier ,  Patrick Treado

发明人： Shona Stewart ,  John Maier ,  Patrick Treado

IPC分类号： G01J3/44

CPC分类号： G01N21/65 ,  G01J3/2823 ,  G01J3/44 ,  G01N21/658 ,  G01N33/1826

摘要： A method for assessing the presence of a pathogenic microorganism in a sample. A sample is illuminated to thereby produce a first plurality of interacted photons which may be scattered, emitted, reflected and/or absorbed by the sample. The first plurality of interacted photons are assessed to thereby generate a Raman data set representative of the sample. This Raman data set is analyzed to thereby determine at least one of: the presence of a pathogenic microorganism in said sample and the absence of a pathogenic microorganism in said sample. The Raman data set may comprise at least one of a Raman spectrum and/or a Raman chemical image representative of the sample. The analysis may comprise comparing said Raman data set to at least one reference Raman data set representative of a known sample. This may be achieved using a chemometric technique.

摘要翻译： 用于评估样品中病原微生物的存在的方法。 照射样品从而产生可被样品散射，发射，反射和/或吸收的第一多个相互作用的光子。 评估第一组多个相互作用的光子，从而产生代表样品的拉曼数据集。 分析该拉曼数据集，从而确定所述样品中病原微生物的存在和所述样品中不存在致病微生物中的至少一种。 拉曼数据集可以包括代表样品的拉曼光谱和/或拉曼化学图像中的至少一个。 分析可以包括将所述拉曼数据集与代表已知样本的至少一个参考拉曼数据集进行比较。 这可以使用化学计量技术来实现。

公开(公告)号：US20060281068A1

公开(公告)日：2006-12-14

申请号：US11269596

申请日：2005-11-09

申请人： John Maier ,  Shona Stewart ,  Patrick Treado

发明人： John Maier ,  Shona Stewart ,  Patrick Treado

IPC分类号： C12Q1/00 ,  C12Q1/70 ,  C12Q1/68 ,  G06F19/00 ,  G01J3/44

CPC分类号： G01N21/65 ,  G01N2021/656

摘要： Raman molecular imaging (RMI) is used to detect mammalian cells of a particular phenotype. For example the disclosure includes the use of RMI to differentiate between normal and diseased cells or tissues, e.g., cancer cells as well as in determining the grade of said cancer cells. In a preferred embodiment benign and malignant lesions of bladder and other tissues can be distinguished, including epithelial tissues such as lung, prostate, kidney, breast, and colon, and non-epithelial tissues, such as bone marrow and brain. Raman scattering data relevant to the disease state of cells or tissue can be combined with visual image data to produce hybrid images which depict both a magnified view of the cellular structures and information relating to the disease state of the individual cells in the field of view. Also, RMI techniques may be combined with visual image data and validated with other detection methods to produce confirm the matter obtained by RMI.

摘要翻译： 拉曼分子成像（RMI）用于检测特定表型的哺乳动物细胞。 例如，本公开包括使用RMI来区分正常和患病细胞或组织，例如癌细胞，以及确定所述癌细胞的等级。 在一个优选实施方案中，可以区分膀胱和其他组织的良性和恶性病变，包括上皮组织如肺，前列腺，肾，乳腺和结肠以及非上皮组织，例如骨髓和脑。 可以将与细胞或组织的疾病状态相关的拉曼散射数据与视觉图像数据组合，以产生描绘细胞结构的放大视图和与视野中的各个细胞的疾病状态有关的信息的混合图像。 此外，RMI技术可以与可视图像数据组合，并用其他检测方法验证，以确定RMI获得的事项。

公开(公告)号：US20050052645A1

公开(公告)日：2005-03-10

申请号：US10922006

申请日：2004-08-18

申请人： Shona Stewart ,  John Maier ,  Patrick Treado

发明人： Shona Stewart ,  John Maier ,  Patrick Treado

IPC分类号： C12Q1/04 ,  G01N21/31 ,  G01N21/64 ,  G01N21/65 ,  G01J3/44

CPC分类号： G01N21/6486 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/2823 ,  G01J3/44 ,  G01N21/31 ,  G01N21/33 ,  G01N21/359 ,  G01N21/645 ,  G01N21/6456 ,  G01N21/6458 ,  G01N21/65 ,  G01N2021/1736 ,  G01N2021/6421 ,  G01N2021/6423 ,  G01N2021/656

摘要： Raman scattering of radiation applied to a water sample is used to assess occurrence of a pathogen in the sample. The method is useful for detecting pathogens that are difficult to detect using other methods, such as protozoa. Examples of organisms that can be detected in water samples using these methods include protozoa of the genus Cryptosporidium and the genus Giardia. The methods described herein have important applications, such as for detection of Cryptosporidium organisms in municipal water systems.