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公开(公告)号:US20120149012A1
公开(公告)日:2012-06-14
申请号:US13180382
申请日:2011-07-11
申请人: Shoulian Dong , Junko F. Stevens
发明人: Shoulian Dong , Junko F. Stevens
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6827 , C12Q2537/164 , C12Q2522/101
摘要: The present teachings provide DNA methylation quantification methods that avoid bisulfite treatment of DNA. Methylation-specific binding proteins (MeDNA binding proteins) and non-methylation specific binding proteins (non-MeDNA binding proteins) are employed in various embodiments to modulate the accessibility of nucleic acids to primer extension reactions. After selectively removing the target nucleic acids, the extension products can be analyzed and methylation quantitated. In some embodiments, the analysis comprises real-time PCR.
摘要翻译: 本教导提供了避免亚硫酸氢盐处理DNA的DNA甲基化定量方法。 甲基化特异性结合蛋白(MeDNA结合蛋白)和非甲基化特异性结合蛋白(非MeDNA结合蛋白)用于各种实施方案中以调节核酸对引物延伸反应的可及性。 在选择性除去靶核酸后,可以分析延伸产物并定量甲基化。 在一些实施方案中,分析包括实时PCR。
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公开(公告)号:US20100041057A1
公开(公告)日:2010-02-18
申请号:US12543450
申请日:2009-08-18
申请人: Shoulian Dong , Junko F. Stevens
发明人: Shoulian Dong , Junko F. Stevens
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6827 , C12Q2537/164 , C12Q2522/101
摘要: The present teachings provide DNA methylation quantification methods that avoid bisulfite treatment of DNA. Methylation-specific binding proteins (MeDNA binding proteins) and non-methylation specific binding proteins (non-MeDNA binding proteins) are employed in various embodiments to modulate the accessibility of nucleic acids to primer extension reactions. After selectively removing the target nucleic acids, the extension products can be analyzed and methylation quantitated. In some embodiments, the analysis comprises real-time PCR.
摘要翻译: 本教导提供了避免亚硫酸氢盐处理DNA的DNA甲基化定量方法。 在各种实施方案中使用甲基化特异性结合蛋白(MeDNA结合蛋白)和非甲基化特异性结合蛋白(非MeDNA结合蛋白)来调节核酸对引物延伸反应的可及性。 在选择性除去靶核酸后,可以分析延伸产物并定量甲基化。 在一些实施方案中,分析包括实时PCR。
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公开(公告)号:US20130084574A1
公开(公告)日:2013-04-04
申请号:US13617230
申请日:2012-09-14
申请人: Shoulian Dong , Junko F. Stevens , Chunmei Liu , Cora L. Woo , Luz Montesclaros
发明人: Shoulian Dong , Junko F. Stevens , Chunmei Liu , Cora L. Woo , Luz Montesclaros
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6806 , C12Q1/6827 , C12Q1/6848 , C12Q1/6886 , C12Q2600/154 , C12Q2600/158 , C12Q2600/178 , C12Q2523/101 , C12Q2537/164 , C12Q2565/133 , C12Q2545/113 , C12Q2525/173 , C12Q2525/191
摘要: Disclosed herein are compositions, methods and kits for analyzing three-dimensional chromatin and/or chromosome conformation. Method are also disclosed for using the methods disclosed herein for diagnosing diseases such as cancer.
摘要翻译: 本文公开了用于分析三维染色质和/或染色体构象的组合物,方法和试剂盒。 还公开了使用本文公开的用于诊断疾病如癌症的方法的方法。
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公开(公告)号:US08663919B2
公开(公告)日:2014-03-04
申请号:US13475089
申请日:2012-05-18
申请人: Shoulian Dong , Junko F. Stevens , Chunmei Liu , Cora L. Woo , Luz Montesclaros
发明人: Shoulian Dong , Junko F. Stevens , Chunmei Liu , Cora L. Woo , Luz Montesclaros
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6806 , C12Q1/6827 , C12Q1/6848 , C12Q1/6886 , C12Q2600/154 , C12Q2600/158 , C12Q2600/178 , C12Q2523/101 , C12Q2537/164 , C12Q2565/133 , C12Q2545/113 , C12Q2525/173 , C12Q2525/191
摘要: Disclosed herein are compositions, methods and kits for analyzing three-dimensional chromatin and/or chromosome conformation. Method are also disclosed for using the methods disclosed herein for diagnosing diseases such as cancer.
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公开(公告)号:US20120302449A1
公开(公告)日:2012-11-29
申请号:US13475089
申请日:2012-05-18
申请人: Shoulian Dong , Junko F. Stevens , Chunmei Liu , Cora L. Woo , Luz Montesclaros
发明人: Shoulian Dong , Junko F. Stevens , Chunmei Liu , Cora L. Woo , Luz Montesclaros
CPC分类号: C12Q1/6869 , C12Q1/6806 , C12Q1/6827 , C12Q1/6848 , C12Q1/6886 , C12Q2600/154 , C12Q2600/158 , C12Q2600/178 , C12Q2523/101 , C12Q2537/164 , C12Q2565/133 , C12Q2545/113 , C12Q2525/173 , C12Q2525/191
摘要: Disclosed herein are compositions, methods and kits for analyzing three-dimensional chromatin and/or chromosome conformation. Method are also disclosed for using the methods disclosed herein for diagnosing diseases such as cancer.
摘要翻译: 本文公开了用于分析三维染色质和/或染色体构象的组合物,方法和试剂盒。 还公开了使用本文公开的用于诊断疾病如癌症的方法的方法。
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公开(公告)号:US08877440B2
公开(公告)日:2014-11-04
申请号:US13617230
申请日:2012-09-14
申请人: Shoulian Dong , Junko F. Stevens , Chunmei Liu , Cora L. Woo , Luz Montesclaros
发明人: Shoulian Dong , Junko F. Stevens , Chunmei Liu , Cora L. Woo , Luz Montesclaros
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6806 , C12Q1/6827 , C12Q1/6848 , C12Q1/6886 , C12Q2600/154 , C12Q2600/158 , C12Q2600/178 , C12Q2523/101 , C12Q2537/164 , C12Q2565/133 , C12Q2545/113 , C12Q2525/173 , C12Q2525/191
摘要: Disclosed herein are compositions, methods and kits for analyzing three-dimensional chromatin and/or chromosome conformation. Method are also disclosed for using the methods disclosed herein for diagnosing diseases such as cancer.
摘要翻译: 本文公开了用于分析三维染色质和/或染色体构象的组合物,方法和试剂盒。 还公开了使用本文公开的用于诊断疾病如癌症的方法的方法。
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公开(公告)号:US20100113285A1
公开(公告)日:2010-05-06
申请号:US12431685
申请日:2009-04-28
申请人: H. Pin Kao , Ian A. Harding , Kathleen C. Hayashibara , Achim Karger , Fiona C. Laird Hyland , Mark F. Oldham , Omead Ostadan , Mark R. Pratt , Junko F. Stevens , Austin B. Tomaney , Greg Young
发明人: H. Pin Kao , Ian A. Harding , Kathleen C. Hayashibara , Achim Karger , Fiona C. Laird Hyland , Mark F. Oldham , Omead Ostadan , Mark R. Pratt , Junko F. Stevens , Austin B. Tomaney , Greg Young
CPC分类号: G01N35/028 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/523 , B01L9/56 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , C12Q1/686 , C12Q2600/166 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N35/0099 , G01N2035/00356 , G01N2035/042 , G16H10/40 , H04N5/361 , H04N5/367 , C12Q2545/113
摘要: A method of using a standard to correct for variability in sample handling, can comprise (a) adding a template of known concentration to an assay comprising a sample; (b) preamplifying the assay; (c) amplifying the assay; (d) collecting data during the amplifying; and (e) correcting the data using a comparison of data collected from the template to data collected from the sample.
摘要翻译: 使用标准来校正样品处理的变异性的方法可以包括(a)将已知浓度的模板加入到包含样品的测定中; (b)预扩增分析; (c)扩增测定; (d)放大期间收集数据; 和(e)使用从模板收集的数据与从样本收集的数据的比较来校正数据。
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