DNA Methylation Detection Methods
    1.
    发明申请
    DNA Methylation Detection Methods 有权
    DNA甲基化检测方法

    公开(公告)号:US20120149012A1

    公开(公告)日:2012-06-14

    申请号:US13180382

    申请日:2011-07-11

    IPC分类号: C12Q1/68

    摘要: The present teachings provide DNA methylation quantification methods that avoid bisulfite treatment of DNA. Methylation-specific binding proteins (MeDNA binding proteins) and non-methylation specific binding proteins (non-MeDNA binding proteins) are employed in various embodiments to modulate the accessibility of nucleic acids to primer extension reactions. After selectively removing the target nucleic acids, the extension products can be analyzed and methylation quantitated. In some embodiments, the analysis comprises real-time PCR.

    摘要翻译: 本教导提供了避免亚硫酸氢盐处理DNA的DNA甲基化定量方法。 甲基化特异性结合蛋白(MeDNA结合蛋白)和非甲基化特异性结合蛋白(非MeDNA结合蛋白)用于各种实施方案中以调节核酸对引物延伸反应的可及性。 在选择性除去靶核酸后,可以分析延伸产物并定量甲基化。 在一些实施方案中,分析包括实时PCR。

    DNA METHYLATION DETECTION METHODS
    2.
    发明申请
    DNA METHYLATION DETECTION METHODS 审中-公开
    DNA甲基化检测方法

    公开(公告)号:US20100041057A1

    公开(公告)日:2010-02-18

    申请号:US12543450

    申请日:2009-08-18

    IPC分类号: C12Q1/68

    摘要: The present teachings provide DNA methylation quantification methods that avoid bisulfite treatment of DNA. Methylation-specific binding proteins (MeDNA binding proteins) and non-methylation specific binding proteins (non-MeDNA binding proteins) are employed in various embodiments to modulate the accessibility of nucleic acids to primer extension reactions. After selectively removing the target nucleic acids, the extension products can be analyzed and methylation quantitated. In some embodiments, the analysis comprises real-time PCR.

    摘要翻译: 本教导提供了避免亚硫酸氢盐处理DNA的DNA甲基化定量方法。 在各种实施方案中使用甲基化特异性结合蛋白(MeDNA结合蛋白)和非甲基化特异性结合蛋白(非MeDNA结合蛋白)来调节核酸对引物延伸反应的可及性。 在选择性除去靶核酸后,可以分析延伸产物并定量甲基化。 在一些实施方案中,分析包括实时PCR。