公开(公告)号：US20090156427A1

公开(公告)日：2009-06-18

申请号：US12183916

申请日：2008-07-31

申请人： Shuguang Zhang ,  Andreas Mershin ,  Liselotte Kaiser ,  Brian Cook ,  Johanna F. Graveland-Bikker ,  Manu Prakash ,  David Kong ,  Yael Maguire

发明人： Shuguang Zhang ,  Andreas Mershin ,  Liselotte Kaiser ,  Brian Cook ,  Johanna F. Graveland-Bikker ,  Manu Prakash ,  David Kong ,  Yael Maguire

IPC分类号： C40B30/10 ,  B01J19/00 ,  C40B40/10 ,  C40B60/12 ,  C40B30/00 ,  C12P21/00

CPC分类号： G01N33/54366 ,  G01N2333/726

摘要： The invention provides a bio-sensing nanodevice comprising: a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—on a support, a real time receptor-ligand binding detection method, an odorant delivery system and an odorant recognition program. The biologically-derived G-protein coupled receptor can be stabilized on nanotechnology using surfactant peptide. The said nanodevice provides a greater surface area for better precision and sensitivity to odorant detection. The invention further provides a microfluidic chip containing a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—immobilized on a support, and arranged in at least two dimensional microarray system. The invention also provides a method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip. The invention further provides method of fabricating hOR17-4 olfactory receptor.

摘要翻译： 本发明提供一种生物感测纳米装置，其包括：稳定的生物衍生的G蛋白偶联受体 - 支持物上的嗅觉受体，实时受体 - 配体结合检测方法，加味剂递送系统和气味识别程序。 生物衍生的G蛋白偶联受体可以使用表面活性剂肽在纳米技术上稳定。 所述纳米器件提供更大的表面积，以提高气味检测的精度和灵敏度。 本发明进一步提供了一种微流控芯片，其包含稳定的生物衍生的G蛋白偶联受体 - 嗅觉受体 - 固定在载体上，并且布置在至少二维微阵列系统中。 本发明还提供了一种递送加臭剂的方法，其包括在复杂微流体网络中操作气泡的步骤，其中气泡在携带各种气体样品的微流体通道中移动到芯片上的精确位置。 本发明还提供了制备hOR17-4嗅觉受体的方法。

公开(公告)号：US09714941B2

公开(公告)日：2017-07-25

申请号：US12183916

申请日：2008-07-31

申请人： Shuguang Zhang ,  Andreas Mershin ,  Liselotte Kaiser ,  Brian Cook ,  Johanna F. Graveland-Bikker ,  Manu Prakash ,  David Kong ,  Yael Maguire

发明人： Shuguang Zhang ,  Andreas Mershin ,  Liselotte Kaiser ,  Brian Cook ,  Johanna F. Graveland-Bikker ,  Manu Prakash ,  David Kong ,  Yael Maguire

IPC分类号： G01N33/543

CPC分类号： G01N33/54366 ,  G01N2333/726

摘要： The invention provides a bio-sensing nanodevice comprising: a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—on a support, a real time receptor-ligand binding detection method, an odorant delivery system and an odorant recognition program. The biologically-derived G-protein coupled receptor can be stabilized on nanotechnology using surfactant peptide. The said nanodevice provides a greater surface area for better precision and sensitivity to odorant detection. The invention further provides a microfluidic chip containing a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—immobilized on a support, and arranged in at least two dimensional microarray system. The invention also provides a method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip. The invention further provides method of fabricating hOR17-4 olfactory receptor.

公开(公告)号：US20120021932A1

公开(公告)日：2012-01-26

申请号：US13015851

申请日：2011-01-28

申请人： Andreas Mershin ,  Brian Cook ,  Liselotte Kaiser ,  Johanna F. Bikker ,  Yoshikatsu Miura ,  Daisuke Niwa ,  Dai Ohnishi ,  Atsushi Tazuke ,  Shuguang Zhang

发明人： Andreas Mershin ,  Brian Cook ,  Liselotte Kaiser ,  Johanna F. Bikker ,  Yoshikatsu Miura ,  Daisuke Niwa ,  Dai Ohnishi ,  Atsushi Tazuke ,  Shuguang Zhang

IPC分类号： C40B30/04 ,  C40B40/10 ,  G01N21/75 ,  G01N21/55 ,  G01N24/08

CPC分类号： G01N21/553 ,  B82Y30/00 ,  C07K2/00 ,  G01N21/554 ,  G01N33/0031 ,  G01N2201/06113 ,  G01N2201/08

摘要： The invention provides a bio-sensing nanodevice comprising: a stabilized G-protein coupled receptor on a support, a real time receptor-ligand binding detection method, a test composition delivery system and a test composition recognition program. The G-protein coupled receptor can be stabilized using surfactant peptide. The nanodevice provides a greater surface area for better precision and sensitivity to odorant detection. The invention further provides a microfluidic chip containing a stabilized G-protein coupled receptor immobilized on a support, and arranged in at least two dimensional microarray system. The invention also provides a method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip. The invention further provides method of fabricating hOR17-4 olfactory receptor.

摘要翻译： 本发明提供了一种生物感测纳米装置，其包括：载体上的稳定的G-蛋白偶联受体，实时受体 - 配体结合检测方法，测试组合物递送系统和测试组合物识别程序。 G蛋白偶联受体可以使用表面活性肽稳定。 纳米器件提供更大的表面积，以提高气味检测的精度和灵敏度。 本发明进一步提供了一种微流控芯片，其含有固定在载体上的稳定化的G蛋白偶联受体，并且被布置在至少二维微阵列系统中。 本发明还提供了一种递送加臭剂的方法，其包括在复杂微流体网络中操作气泡的步骤，其中气泡在携带各种气体样品的微流体通道中移动到芯片上的精确位置。 本发明还提供了制备hOR17-4嗅觉受体的方法。

公开(公告)号：US08748111B2

公开(公告)日：2014-06-10

申请号：US13015851

申请日：2011-01-28

申请人： Andreas Mershin ,  Brian Cook ,  Liselotte Kaiser ,  Johanna F. Bikker ,  Yoshikatsu Miura ,  Daisuke Niwa ,  Dai Ohnishi ,  Atsushi Tazuke ,  Shuguang Zhang

发明人： Andreas Mershin ,  Brian Cook ,  Liselotte Kaiser ,  Johanna F. Bikker ,  Yoshikatsu Miura ,  Daisuke Niwa ,  Dai Ohnishi ,  Atsushi Tazuke ,  Shuguang Zhang

IPC分类号： G01N33/53 ,  C07K2/00 ,  B82Y30/00 ,  G01N33/00 ,  G01N21/55

CPC分类号： G01N21/553 ,  B82Y30/00 ,  C07K2/00 ,  G01N21/554 ,  G01N33/0031 ,  G01N2201/06113 ,  G01N2201/08

摘要： The invention provides a bio-sensing nanodevice comprising: a stabilized G-protein coupled receptor on a support, a real time receptor-ligand binding detection method, a test composition delivery system and a test composition recognition program. The G-protein coupled receptor can be stabilized using surfactant peptide. The nanodevice provides a greater surface area for better precision and sensitivity to odorant detection. The invention further provides a microfluidic chip containing a stabilized G-protein coupled receptor immobilized on a support, and arranged in at least two dimensional microarray system. The invention also provides a method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip. The invention further provides method of fabricating hOR17-4 olfactory receptor.

摘要翻译： 本发明提供了一种生物感测纳米装置，其包括：载体上的稳定的G-蛋白偶联受体，实时受体 - 配体结合检测方法，测试组合物递送系统和测试组合物识别程序。 G蛋白偶联受体可以使用表面活性肽稳定。 纳米器件提供更大的表面积，以提高气味检测的精度和灵敏度。 本发明进一步提供了一种微流控芯片，其含有固定在载体上的稳定化的G蛋白偶联受体，并且被布置在至少二维微阵列系统中。 本发明还提供了一种递送加臭剂的方法，其包括在复杂微流体网络中操作气泡的步骤，其中气泡在携带各种气体样品的微流体通道中移动到芯片上的精确位置。 本发明还提供了制备hOR17-4嗅觉受体的方法。