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1.发明授权Microscope having a confocal point and a non-confocal point, and a confocal point detect method applied thereto 失效具有共焦点和非共焦点的显微镜和应用于其的共焦点检测方法
公开(公告)号：US5932871A
公开(公告)日：1999-08-03
申请号：US744752
申请日：1996-11-06
申请人： Shuji Nakagawa , Akihiro Kitahara , Asao Uenodai
发明人： Shuji Nakagawa , Akihiro Kitahara , Asao Uenodai
CPC分类号： G02B21/0048 , G02B21/008
摘要： A microscope includes a light source, an objective lens for converging light emitted from the light source onto a sample, and an optical detector for detecting either light from the sample via a confocal point diaphragm or light from the sample which does not pass through the confocal point diaphragm. A range of a focusing point is detected in accordance with the light which does not pass through the confocal diaphragm, and the focusing point is then detected in accordance with the light detected via the confocal point diaphragm in the detected range of the focusing point.
摘要翻译： 显微镜包括光源，用于将从光源发射的光会聚到样品上的物镜，以及用于检测来自样品的来自共焦点膜的光或来自未通过共焦的样品的光的光学检测器 点隔膜。 根据不通过共聚焦膜的光检测聚焦点的范围，然后根据在聚焦点的检测范围内通过共焦点光圈检测到的光来检测聚焦点。
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公开(公告)号：US20050133692A1
公开(公告)日：2005-06-23
申请号：US10990747
申请日：2004-11-17
申请人： Hideo Watanabe , Akihiro Kitahara
发明人： Hideo Watanabe , Akihiro Kitahara
CPC分类号： G02B21/008
摘要： A first amplification unit amplifies at a predetermined amplification rate an output signal from a photodetection unit for detecting an optical signal from a specimen and detecting it. An offset unit assigns offset to an output signal from the photodetection unit. A second amplification unit amplifies at an amplification rate smaller than the amplification rate of the first amplification unit an output signal from the photodetection unit assigned the offset by the offset unit. A rectification unit rectifies an output signal from the second amplification unit. An addition unit adds an output signal from the first amplification unit to an output signal from the rectification unit.
摘要翻译： 第一放大单元以预定的放大率放大来自用于检测来自样本的光信号的光检测单元的输出信号并进行检测。 偏移单元将偏移分配给来自光电检测单元的输出信号。 第二放大单元以比第一放大单元的放大率更小的放大率放大来自由偏移单元分配了偏移的光检测单元的输出信号。 整流单元对来自第二放大单元的输出信号进行整流。 加法单元将来自第一放大单元的输出信号与来自整流单元的输出信号相加。
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公开(公告)号：US07078664B2
公开(公告)日：2006-07-18
申请号：US10986493
申请日：2004-11-10
申请人： Akihiro Kitahara
发明人： Akihiro Kitahara
CPC分类号： G02B21/0048 , G02B21/18 , G02B21/365
摘要： In a confocal laser microscope, a confocal image and a non-confocal image of an observation target are generated from light reflected by the observation target, and the confocal image and non-confocal image are displayed side by side on the same screen.
摘要翻译： 在共焦激光显微镜中，由观察对象反射的光产生观察目标的共焦图像和非共焦图像，并且在同一屏幕上并排显示共焦图像和非共焦图像。
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公开(公告)号：US20050139748A1
公开(公告)日：2005-06-30
申请号：US10986493
申请日：2004-11-10
申请人： Akihiro Kitahara
发明人： Akihiro Kitahara
CPC分类号： G02B21/0048 , G02B21/18 , G02B21/365
摘要： In a confocal laser microscope, a confocal image and a non-confocal image of an observation target are generated from light reflectedby the observation target, and the confocal image and non-confocal image are displayed side by side on the same screen.
摘要翻译： 在共焦激光显微镜中，由观察对象反射的光产生观察对象的共焦图像和非共焦图像，并且在同一屏幕上并排显示共焦图像和非共焦图像。
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公开(公告)号：US20050122577A1
公开(公告)日：2005-06-09
申请号：US11015076
申请日：2004-12-16
CPC分类号： G02B21/006 , G01B9/04 , G02B21/008
摘要： The present invention relates to a confocal microscope and a measuring method by the confocal microscope in which one or a plurality of measuring units to detect a movement amount are disposed facing a Z-stage having a sample laid thereon, the measuring unit detects a relative position between a condensing position of an objective lens and the sample, a maximum value of a change curved line indicated by light intensity information, and a relative position giving this value are estimated based on obtained relative position information, and a plurality of pieces of light intensity information including the maximum light intensity value of the light intensity, and a confocal image is produced using the estimated maximum value of the light intensity and the relative position as reflection luminance information and height information.
摘要翻译： 本发明涉及共聚焦显微镜和通过共聚焦显微镜的测量方法，其中一个或多个检测移动量的测量单元被布置为面向铺设有样品的Z级，测量单元检测相对位置 在物镜的聚光位置和样品之间，基于获得的相对位置信息来估计给出该值的由光强度信息指示的改变曲线的最大值和相对位置，并且多个光强度 使用光强度的估计最大值和相对位置作为反射亮度信息和高度信息来产生包括光强度的最大光强度值和共焦图像的信息。
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公开(公告)号：US20050111787A1
公开(公告)日：2005-05-26
申请号：US10995399
申请日：2004-11-23
CPC分类号： G06K7/10633 , G01S7/4817 , G02B7/1821 , G02B26/085 , G02B26/101 , G02B26/105
摘要： A two-dimensional optical deflector includes a first deflector, which deflects a light beam about a first axis at a high speed, a second deflector, which deflects a light beam about a second axis perpendicular to the first axis at a low speed, and a fixing mechanism, which fixes the first deflector to the second deflector. The second deflector includes a rotation shaft capable of rocking about the second axis. The fixing mechanism includes a fixing member having a rotation shaft fixing hole, which engages with the rotation shaft with almost no space, and a fastener, which decreases the diameter of the rotation shaft fixing hole of the fixing member. The fixing member includes a first deflector mount surface. The first deflector is fixed to the fixing member so that a reflecting surface of the first deflector in a non-actuation state is almost parallel to the first deflector mount surface.
摘要翻译： 二维光学偏转器包括第一偏转器，该第一偏转器绕高速的第一轴线偏转光束;第二偏转器，其以低速度偏转垂直于第一轴线的第二轴线的光束;以及 固定机构，其将第一偏转器固定到第二偏转器。 第二偏转器包括能够绕第二轴摆动的旋转轴。 固定机构包括具有旋转轴固定孔的固定构件，其与几乎没有空间的旋转轴接合，并且紧固件减小了固定构件的旋转轴固定孔的直径。 固定构件包括第一偏转器安装表面。 第一偏转器固定到固定构件，使得第一偏转器的反作用力处于非致动状态，几乎平行于第一偏转器安装表面。
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公开(公告)号：US08041097B2
公开(公告)日：2011-10-18
申请号：US12208536
申请日：2008-09-11
申请人： Shuji Akiyama , Akihiro Kitahara
发明人： Shuji Akiyama , Akihiro Kitahara
IPC分类号： G06K9/00
CPC分类号： G02B21/008 , G02B21/006 , G06T5/50 , G06T2207/10056
摘要： To provide a confocal microscope for obtaining an extended image by easily determining the capturing range of an observation image without being aware of the shape of a sample to be observed, the confocal microscope comprises confocal image generating unit for generating a confocal image, first counting unit for counting a first number of pixels having a predetermined brightness level or lower among the brightness levels of the pixels of the confocal image, second counting unit for counting a second number of pixels obtained by extracting only a pixel that matches a predetermined condition for the confocal image, and boundary determining unit for detecting a boundary by determining whether or not the observation surface is within the image capturing range based on the first and the second numbers of pixels.
摘要翻译： 为了提供共焦显微镜，通过容易地确定观察图像的捕获范围而不知道要观察的样品的形状来获得扩展图像，共聚焦显微镜包括用于产生共聚焦图像的共焦图像产生单元，第一计数单元 用于计数在共焦图像的像素的亮度级中具有预定亮度级别或更低的第一数量的像素;第二计数单元，用于对仅通过提取与共焦焦点的预定条件匹配的像素而获得的第二数量的像素进行计数; 图像和边界确定单元，用于通过基于第一和第二像素数量确定观察表面是否在图像捕获范围内来检测边界。
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公开(公告)号：US07297921B2
公开(公告)日：2007-11-20
申请号：US10990747
申请日：2004-11-17
申请人： Hideo Watanabe , Akihiro Kitahara
发明人： Hideo Watanabe , Akihiro Kitahara
CPC分类号： G02B21/008
摘要： A first amplification unit amplifies at a predetermined amplification rate an output signal from a photodetection unit for detecting an optical signal from a specimen and detecting it. An offset unit assigns offset to an output signal from the photodetection unit. A second amplification unit amplifies at an amplification rate smaller than the amplification rate of the first amplification unit an output signal from the photodetection unit assigned the offset by the offset unit. A rectification unit rectifies an output signal from the second amplification unit. An addition unit adds an output signal from the first amplification unit to an output signal from the rectification unit.
摘要翻译： 第一放大单元以预定的放大率放大来自用于检测来自样本的光信号的光检测单元的输出信号并进行检测。 偏移单元将偏移分配给来自光电检测单元的输出信号。 第二放大单元以比第一放大单元的放大率更小的放大率放大来自由偏移单元分配了偏移的光检测单元的输出信号。 整流单元对来自第二放大单元的输出信号进行整流。 加法单元将来自第一放大单元的输出信号与来自整流单元的输出信号相加。
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公开(公告)号：US20070194206A1
公开(公告)日：2007-08-23
申请号：US11788914
申请日：2007-04-23
申请人： Akihiro Kitahara
发明人： Akihiro Kitahara
IPC分类号： G02B27/40
CPC分类号： G02B21/0024 , G02B21/365
摘要： A confocal laser scanning microscope acquires confocal images of a sample. The microscope is provided with an optical-microscope optical system which acquires non-confocal images of the sample by detecting measurement light coming from the sample. The optical-microscope optical system includes optical systems corresponding to at least two observation methods, and one of the optical systems is selected during use.
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公开(公告)号：US20050161593A1
公开(公告)日：2005-07-28
申请号：US10992421
申请日：2004-11-18
申请人： Akihiro Kitahara
发明人： Akihiro Kitahara
CPC分类号： G02B21/0024 , G02B21/365
摘要： A confocal laser scanning microscope acquires confocal images of a sample. The microscope is provided with an optical-microscope optical system which acquires non-confocal images of the sample by detecting measurement light coming from the sample. The optical-microscope optical system includes optical systems corresponding to at least two observation methods, and one of the optical systems is selected during use.
摘要翻译： 共焦激光扫描显微镜获取样品的共聚焦图像。 显微镜配备有光学显微镜光学系统，其通过检测来自样品的测量光来获取样品的非共焦图像。 光学显微镜光学系统包括对应于至少两种观察方法的光学系统，并且在使用期间选择一个光学系统。
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