公开(公告)号：US20060229735A1

公开(公告)日：2006-10-12

申请号：US11250287

申请日：2005-10-14

申请人： Shuvo Roy ,  George Muschler ,  Aaron Fleischman ,  Alvaro Mata

发明人： Shuvo Roy ,  George Muschler ,  Aaron Fleischman ,  Alvaro Mata

IPC分类号： A61F2/02 ,  C12N5/08

CPC分类号： A61F2/0077 ,  A61K35/35 ,  A61L27/3843 ,  A61L27/50 ,  C12N5/0068 ,  C12N5/0654 ,  C12N2535/10

摘要： An implant includes a plurality of parallel layers spaced apart by a plurality of members. Each layer has a substantially uniform thickness between opposite surfaces and a plurality of openings that permit fluid flow through an interior of the implant defined by the layers. The surfaces of each layer includes an array of micro-structures. Each micro-structure has a substantially uniform shape and an average height of about 1 nm to about 20 μm.

摘要翻译： 植入物包括由多个构件间隔开的多个平行层。 每个层在相对表面之间具有基本均匀的厚度，并且允许流体流过由层限定的植入物的内部的多个开口。 每个层的表面包括微结构的阵列。 每个微结构具有基本均匀的形状和约1nm至约20μm的平均高度。

公开(公告)号：US20060280352A1

公开(公告)日：2006-12-14

申请号：US11450125

申请日：2006-06-09

申请人： George Muschler ,  Kimerly Powell ,  Jason Bryan

发明人： George Muschler ,  Kimerly Powell ,  Jason Bryan

IPC分类号： G06K9/00

CPC分类号： G06K9/0014 ,  G06T7/0012 ,  G06T7/11 ,  G06T2207/10056 ,  G06T2207/20041 ,  G06T2207/30024

摘要： Systems and methods are described for performing image analysis. A computer-implemented method for analyzing images may include quantitatively analyzing image data to identify image objects relative to a background portion of the image according to predefined object criteria, the image data including a plurality of image objects that represent objects in a sample distributed across a substrate. The identified image objects are further clustered into groups or colonies of the identified image objects according to predetermined clustering criteria.

摘要翻译： 描述了进行图像分析的系统和方法。 用于分析图像的计算机实现的方法可以包括定量分析图像数据，以根据预定义的对象标准来相对于图像的背景部分识别图像对象，所述图像数据包括表示分布在图像中的样本中的对象的多个图像对象 基质。 根据预定的聚类标准，所识别的图像对象被进一步聚类成所识别的图像对象的组或群集。

公开(公告)号：US09045735B2

公开(公告)日：2015-06-02

申请号：US12594493

申请日：2008-04-03

申请人： George Muschler ,  Tonya Caralla ,  Vincent Hascall ,  Ronald Midura

发明人： George Muschler ,  Tonya Caralla ,  Vincent Hascall ,  Ronald Midura

IPC分类号： C12N5/02 ,  C12N5/071 ,  C12N5/00 ,  C12N5/0775

CPC分类号： C12N5/0663 ,  C12N2500/38 ,  C12N2501/39 ,  C12N2501/905 ,  C12N2533/80

摘要： Methods for enriching, detecting, or using adult stem cells through the use of recognition ligands that specifically bind to ECM components retained to the surfaces of adult stem cells are described. An ECM component such as hyaluronan that is retained to the surfaces of adult stem cells when removed from animal tissues can be used to detect a diverse population of adult stem cells based on the nature of the ECM niche region in which the adult stem cells normally reside. For example, a separation method such as magnetic separation can be used to detect and isolate or enrich adult stem cells based on a recognition ligand that is specific for an ECM component that is retained to the surfaces of adult stem cells to a greater degree than to other cells in the population.

摘要翻译： 描述了通过使用特异性结合保留在成体干细胞表面的ECM成分的识别配体来丰富，检测或使用成体干细胞的方法。 根据成体干细胞通常存在的ECM特征区的性质，可以使用从动物组织中除去时保留在成体干细胞表面的诸如透明质酸的ECM组分，以检测成体干细胞的不同群体 。 例如，可以使用诸如磁性分离的分离方法来检测和分离或富集成体干细胞，其基于识别配体，其对于保留在成体干细胞表面的ECM成分特异性更大程度上 人口中的其他细胞。

公开(公告)号：US20070055282A1

公开(公告)日：2007-03-08

申请号：US11595472

申请日：2006-11-09

申请人： George Muschler

发明人： George Muschler

IPC分类号： A61F2/00 ,  A61B10/00

CPC分类号： A61B10/0233 ,  A61B10/0045 ,  A61B10/025 ,  A61B10/0283 ,  A61B17/1635 ,  A61B17/1664 ,  A61B17/32002 ,  A61B2010/0258 ,  A61B2217/005 ,  A61B2217/007

摘要： A minimally invasive apparatus and method for harvesting bone marrow cells, blood, and bone fragments includes a rigid cannula having a proximal end and a distal end with an opening. The distal end includes a cutting tip that is movable axially and radially to cut and disrupt bone tissue while preserving necessary viability among harvested marrow cells. The cannula further includes an inner surface defining an internal passage that extends from the opening toward the proximal end. Suction is applied to the passage to draw disrupted bone marrow cells, blood, and bone fragments into the internal passage for collection. The apparatus may include a rotatable shaft disposed co-axially within the internal passage. The shaft has a distal end with a cutting bit for further cutting and disrupting of bone tissue, and a lumen for supplying bone cement to the cutting bit to promote bone growth and healing within the bone.

摘要翻译： 用于收获骨髓细胞，血液和骨碎片的微创装置和方法包括具有近端和具有开口的远端的刚性插管。 远端包括可沿轴向和径向移动以切割和破坏骨组织的切割尖端，同时在收获的骨髓细胞之间保持必要的活力。 套管还包括限定从开口朝向近端延伸的内部通道的内表面。 吸入通道，将破碎的骨髓细胞，血液和骨碎片引入内部通道进行收集。 该设备可以包括同轴地设置在内部通道内的可旋转轴。 轴具有远端，其具有用于进一步切割和破坏骨组织的切削刀头，以及用于将骨水泥供应到切削刀头以促进骨骼内的骨生长和愈合的管腔。

公开(公告)号：US20100167316A1

公开(公告)日：2010-07-01

申请号：US12594493

申请日：2008-04-03

申请人： George Muschler ,  Tonya Caralla ,  Vincent Hascall ,  Ronald Midura

发明人： George Muschler ,  Tonya Caralla ,  Vincent Hascall ,  Ronald Midura

IPC分类号： G01N33/567 ,  C12N5/02

CPC分类号： C12N5/0663 ,  C12N2500/38 ,  C12N2501/39 ,  C12N2501/905 ,  C12N2533/80

摘要： Methods for enriching, detecting, or using adult stem cells through the use of recognition ligands that specifically bind to ECM components retained to the surfaces of adult stem cells are described. An ECM component such as hyaluronan that is retained to the surfaces of adult stem cells when removed from animal tissues can be used to detect a diverse population of adult stem cells based on the nature of the ECM niche region in which the adult stem cells normally reside. For example, a separation method such as magnetic separation can be used to detect and isolate or enrich adult stem cells based on a recognition ligand that is specific for an ECM component that is retained to the surfaces of adult stem cells to a greater degree than to other cells in the population.

摘要翻译： 描述了通过使用特异性结合保留在成体干细胞表面的ECM成分的识别配体来丰富，检测或使用成体干细胞的方法。 根据成体干细胞通常存在的ECM特征区的性质，可以使用从动物组织中除去时保留在成体干细胞表面的诸如透明质酸的ECM组分，以检测成体干细胞的不同群体 。 例如，可以使用诸如磁性分离的分离方法来检测和分离或富集成体干细胞，其基于识别配体，其对于保留在成体干细胞表面的ECM成分特异性更大程度上 人口中的其他细胞。