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    Method for immobolizing molecules on surfaces
    1.
    发明申请
    Method for immobolizing molecules on surfaces 审中-公开
    表面上固定分子的方法

    公开(公告)号：US20050158848A1

    公开(公告)日：2005-07-21

    申请号：US10511192

    申请日：2003-04-10

    申请人： Ulrich Sieben ,  Ingo Freund ,  Holger Klapproth

    发明人： Ulrich Sieben ,  Ingo Freund ,  Holger Klapproth

    IPC分类号： B01J20/32 ,  C09D125/06 ,  C09D179/08 ,  C09D201/00 ,  G01N33/543 ,  G01N37/00 ,  C12M1/34 ,  B05D3/00

    CPC分类号： B01J20/3204 ,  B01J20/103 ,  B01J20/28011 ,  B01J20/3257 ,  B01J20/327 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3276 ,  B01J20/3289 ,  G01N33/54393

    摘要： In a method for immobilizing molecules on a surface, a layer of a hydrophobic, e.g., a non-swelling polymer, may be applied to the surface and molecules are immobilized on a surface of the polymer layer. The hydrophobic polymers may be polyimide or polystyrene, and the surface to which the polymer layer is applied may be made of an inorganic material, e.g., a semiconductor material (silicon), a semiconducting oxide (silicon dioxide), glass, nitride, or ceramic. Hydrophobic polymers such as polyimide or polystyrene can be applied to the surface of an inorganic support with conventional methods. Further, they electrically insulate the support with respect to the molecules applied to the surface of the polymer layer or substances associated with these molecules. Thus, electrical sensors and processor circuits can be integrated into the support without any detrimental effect on their function due to molecules and substances applied to the surface of the polymer layer.

    摘要翻译： 在将分子固定在表面上的方法中，可以将一层疏水的，例如不溶胀的聚合物施加到表面上，并将分子固定在聚合物层的表面上。 疏水聚合物可以是聚酰亚胺或聚苯乙烯，并且施加聚合物层的表面可以由无机材料制成，例如半导体材料（硅），半导体氧化物（二氧化硅），玻璃，氮化物或陶瓷 。 疏水性聚合物如聚酰亚胺或聚苯乙烯可以用常规方法施加到无机载体的表面上。 此外，它们相对于施加到聚合物层的表面上的分子或与这些分子相关联的物质将载体电绝缘。 因此，电传感器和处理器电路可以集成到支撑件中，而不会由于施加到聚合物层表面的分子和物质而对它们的功能产生不利影响。

    Process and device for mixing microdroplets
    3.
    发明申请
    Process and device for mixing microdroplets 审中-公开
    混合微滴的方法和装置

    公开(公告)号：US20090017505A1

    公开(公告)日：2009-01-15

    申请号：US11805242

    申请日：2007-05-22

    申请人： Ulrich Sieben ,  Holger Klapproth

    发明人： Ulrich Sieben ,  Holger Klapproth

    IPC分类号： C12P19/34 ,  C12M1/40 ,  C12M1/34

    CPC分类号： B01L3/0262 ,  B01F13/0071 ,  B01F13/0084 ,  B01F15/06 ,  B01L3/5085 ,  B01L3/5088 ,  B01L3/563 ,  B01L2200/025 ,  B01L2200/143 ,  B01L2300/0819 ,  B01L2300/1822

    摘要： In a process for mixing microdroplets (2a, 2b, 2c), at least two carriers (3a, 3b) are provided whose surfaces (4a, 4b) are each structured in such a way that at least one hydrophilic surface domain (5a, 5b) is delimited by at least one hydrophobic surface domain (6a, 6b). A first microdroplet (2a, 2b, 2c) is disposed on a hydrophilic surface domain (5a) of a first carrier (3a), and a second microdroplet (2a, 2b, 3c) is disposed on a hydrophilic surface domain (5b) of a second carrier (3b). The carriers (3a, 3b), with their first and second hydrophilic surfaces (4a, 4b) facing each other, are positioned adjacent to each other and sufficiently close to each other by being moved toward each other that the microdroplets (2a, 2b, 2c) come into contact with each other.

    摘要翻译： 在混合微滴（2a，2b，2c）的方法中，提供至少两个载体（3a，3b），其表面（4a，4b）各自构造成使得至少一个亲水性表面区域（5a，5b） ）由至少一个疏水性表面结构域（6a，6b）界定。 第一微滴（2a，2b，2c）设置在第一载体（3a）的亲水性表面区域（5a）上，第二微滴（2a，2b，3c）设置在亲水性表面区域 第二载体（3b）。 其第一和第二亲水表面（4a，4b）彼此面对的载体（3a，3b）通过彼此移动彼此相邻并且彼此靠近彼此，使得微滴（2a，2b， 2c）相互接触。

    Method and Apparatus for Measuring the Concentration of a Ligand Contained in a Sample That Is To Be Tested
    4.
    发明申请
    Method and Apparatus for Measuring the Concentration of a Ligand Contained in a Sample That Is To Be Tested 审中-公开
    用于测量待测试样品中的配体浓度的方法和装置

    公开(公告)号：US20080286882A1

    公开(公告)日：2008-11-20

    申请号：US11960090

    申请日：2007-12-19

    申请人： Ulrich Sieben ,  Holger Klapproth

    发明人： Ulrich Sieben ,  Holger Klapproth

    IPC分类号： G01N33/543 ,  B01J19/00

    CPC分类号： G01N33/54366

    摘要： In a method for measuring the concentration of a ligand contained in a sample that is to be tested, at a plurality of test sites that are located on the surface of at least one substrate, in each case at least one receptor that is suitable upon contact with the ligand for specifically binding to said ligand, is immobilized. The sample is brought into contact for a predetermined time with the test sites in such a way that in the sample differently sized diffusion volumes are adjacent to the receptors from which at least one ligand can diffuse to the respective receptor during the predetermined time. After the predetermined time has passed, for each test site a measured signal for the number or density of the binding events at the test site is acquired.

    摘要翻译： 在用于测量待测试样品中所含配体浓度的方法中，在位于至少一个底物表面上的多个测试位点，在每种情况下至少一种适合接触的受体 与特异性结合所述配体的配体被固定化。 使样品与测试位置接触预定时间，使得在样品中不同尺寸的扩散体积与受体相邻，在预定时间内至少一种配体可以从该受体扩散到相应的受体。 在预定时间过去之后，对于每个测试站点，获取测试点处的绑定事件的数量或密度的测量信号。

    Integrated sensor arrangement
    5.
    发明申请
    Integrated sensor arrangement 审中-公开
    集成传感器布置

    公开(公告)号：US20070107514A1

    公开(公告)日：2007-05-17

    申请号：US11545778

    申请日：2006-10-10

    申请人： Ulrich Sieben ,  Holger Klapproth ,  Mirko Lehmann

    发明人： Ulrich Sieben ,  Holger Klapproth ,  Mirko Lehmann

    IPC分类号： G01F15/14

    CPC分类号： G01N21/6454

    摘要： An integrated sensor array has a substrates, at least one sensor element, and a signal processing device integrated in the substrate and connected or capable of being connected with the sensor elements. A layer having a measuring surface is capable of being detachably bonded to the substrate so that a signal emitted from the measuring surface is capable of being processed by the signal processing device when the layer is bonded to the substrate. The substrate has at least one feedthrough hole abutting with the layer, which feedthrough hole is connected or capable of being connected to a vacuum generating device in order to bond the layer to the substrate by suction.

    摘要翻译： 集成传感器阵列具有基板，至少一个传感器元件和集成在基板中并连接或能够与传感器元件连接的信号处理装置。 具有测量表面的层能够可拆卸地结合到基板，使得当该层与基板结合时，从测量表面发射的信号能够被信号处理装置处理。 衬底具有与该层邻接的至少一个馈通孔，所述馈通孔连接或能够连接到真空产生装置，以便通过抽吸将层粘合到衬底。

    Apparatus for intracellular manipulation of a biological cell
    6.
    发明授权
    Apparatus for intracellular manipulation of a biological cell 失效
    用于细胞内操作生物细胞的装置

    公开(公告)号：US07501276B2

    公开(公告)日：2009-03-10

    申请号：US10202419

    申请日：2002-07-23

    申请人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jürgen Gahle ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

    发明人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jürgen Gahle ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

    IPC分类号： C12M1/42 ,  C12N15/87

    CPC分类号： C12N15/89 ,  C12M35/02 ,  C12M35/04 ,  C12M35/08 ,  C12N13/00

    摘要： A process is provided for the intracellular manipulation of a biological cell (3) which is positioned adhering to a support area (5) in a culture medium (2). Inside the support area (5) for the cell (3) an opening into the membrane of the cell (3) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area (5), thus seals off the cell fluid situated in the interior of the cell (3) from the culture medium (2) and insulates the cell fluid against the culture medium (2). The interior of the cell (3) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier (4) with a support area (5) for adhering the cell and a poration tool (6) for creating the opening in the cell membrane. The poration tool (6) may be any of various chemical, mechanical and/or electrical devices.

    摘要翻译： 提供了一种用于细胞内操作生物细胞（3）的方法，所述生物细胞（3）定位为粘附在培养基（2）中的支持区域（5）。 在电池（3）的支撑区域（5）的内部，与电池（3）的隔膜形成的开口与其支撑边缘间隔开。 围绕开口的细胞膜的边缘附着在支撑区域（5）上，从而将位于细胞（3）内部的细胞液体与培养基（2）密封在一起，并使细胞液体抵抗培养物 中等（2）。 电池（3）的内部通过开口被操纵。 还提供了一种用于实现该方法的装置，包括具有用于粘附细胞的支撑区域（5）的物体载体（4）和用于在细胞膜中形成开口的引锭工具（6）。 引锭工具（6）可以是各种化学，机械和/或电气装置中的任何一种。

    Method for intracellular manipulation of a biological cell
    8.
    发明授权
    Method for intracellular manipulation of a biological cell 有权
    细胞内操作生物细胞的方法

    公开(公告)号：US06475760B1

    公开(公告)日：2002-11-05

    申请号：US09321051

    申请日：1999-05-27

    申请人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jürgen Gahle ,  Bernhard Wolf ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

    发明人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jürgen Gahle ,  Bernhard Wolf ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

    IPC分类号： C12N1300

    CPC分类号： C12N15/89 ,  C12M35/02 ,  C12M35/04 ,  C12M35/08 ,  C12N13/00

    摘要： A process is provided for the intracellular manipulation of a biological cell (3) which is positioned adhering to a support area (5) in a culture medium (2). Inside the support area (5) for the cell (3) an opening into the membrane of the cell (3) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area (5), thus seals off the cell fluid situated in the interior of the cell (3) from the culture medium (2) and insulates the cell fluid against the culture medium (2). The interior of the cell (3) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier (4) with a support area (5) for adhering the cell and a poration tool (6) for creating the opening in the cell membrane. The poration tool (6) may be any of various chemical, mechanical and/or electrical devices.

    摘要翻译： 提供了一种用于细胞内操作生物细胞（3）的方法，所述生物细胞（3）定位为粘附在培养基（2）中的支持区域（5）。 在电池（3）的支撑区域（5）的内部，与电池（3）的隔膜形成的开口与其支撑边缘间隔开。 围绕开口的细胞膜的边缘附着在支撑区域（5）上，从而将位于细胞（3）内部的细胞液体与培养基（2）密封并使细胞液体抵抗培养物 中等（2）。 电池（3）的内部通过开口被操纵。 还提供了一种用于实现该方法的装置，包括具有用于粘附细胞的支撑区域（5）的物体载体（4）和用于在细胞膜中形成开口的引锭工具（6）。 引锭工具（6）可以是各种化学，机械和/或电气装置中的任何一种。

    Local communications device
    10.
    发明授权
    Local communications device 失效
    本地通信设备

    公开(公告)号：US06754468B1

    公开(公告)日：2004-06-22

    申请号：US09302391

    申请日：1999-04-30

    申请人： Ulrich Sieben ,  Miodrag Temerinac

    发明人： Ulrich Sieben ,  Miodrag Temerinac

    IPC分类号： H04B700

    CPC分类号： H04M1/7253

    摘要： A Portable communications device (1), particularly as an additional functional unit in a radiotelephone (100), comprising an input, output radio interface (2) for local data exchange between two adjacent communications devices (1; 100) or between a communications device (1; 100) and a data processing device (12) adapted to the,local data exchange, or a data-reproducing device (13), or an adapted data source (50). The range (r) of the input/output radio interface (2) is short compared with the range of a conventional radio interface for data :transfer, and corresponds essentially to a talking distance. The communications device (1, 100) further includes a memory device (6) and a data processing device (5). Different data sequences are identified and selectively retrievable in the memory device (6). The activation of the input/output radio interface (2) can be initiated manually.

    摘要翻译： 一种便携式通信设备（1），特别是作为无线电话（100）中的附加功能单元，包括用于两个相邻通信设备（1; 100）之间或通信设备之间的本地数据交换的输入，输出无线电接口（2） （1; 100）和适于本地数据交换的数据处理设备（12）或数据再现设备（13）或适配的数据源（50）。 输入/输出无线电接口（2）的范围（r）与用于数据的传统无线电接口的范围相比较短：传输，并且基本上对应于通话距离。 通信设备（1,100）还包括存储设备（6）和数据处理设备（5）。 在存储器件（6）中识别并选择性地检索不同的数据序列。 可以手动启动输入/输出无线电接口（2）的激活。