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公开(公告)号：US10545083B2
公开(公告)日：2020-01-28
申请号：US15502630
申请日：2015-08-07
Applicant: Sphere Fluidics Limited
Inventor： Clive Adrian Smith , Xin Li , Frank F. Craig
Abstract: We describe a method comprising: providing a droplet comprising a plurality of constituents, splitting said droplet into a first droplet and a second droplet, wherein said first droplet comprises a first fraction of said plurality of constituents and said second droplet comprises a second fraction of said plurality of constituents, analysing said constituents of said first fraction of said plurality of constituents in said first droplet, and sorting said second droplet dependent on an outcome of said analysis.
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公开(公告)号：US11656167B2
公开(公告)日：2023-05-23
申请号：US16716369
申请日：2019-12-16
Applicant: Sphere Fluidics Limited
Inventor： Clive Adrian Smith , Xin Li , Frank F. Craig
CPC classification number: G01N15/1031 , B01L3/50273 , B01L3/502715 , B01L3/502784 , G01N15/1056 , G01N15/1404 , B01L2200/0652 , B01L2300/0627 , B01L2300/0816 , B01L2300/0864 , B01L2400/0424 , B01L2400/0427 , B01L2400/084 , G01N2015/1081 , G01N2015/149 , G01N2015/1486 , H01J49/0031 , H01J49/04
Abstract: A method for aligning sequences of droplets in streams of an emulsion comprising target droplets and tag droplets, a tag droplet comprising first and second tags. A target droplet is split into first and second target droplets and a tag droplet is split into first and second tag droplets. Each of the first and second tag droplets comprise the first and second tags. The first target droplet and first tag droplet are in a first stream of droplets, and the second target droplet and second tag droplet are in a second stream of droplets. The method detects the first tag droplets and first target droplets in the first stream and the second tag droplets and second target droplets in the second stream, determines a first sequence of droplets in the first stream and a second sequence of droplets in the second stream, and compares these to align the sequences.
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公开(公告)号：US20230243729A1
公开(公告)日：2023-08-03
申请号：US18133980
申请日：2023-04-12
Applicant: Sphere Fluidics Limited
Inventor： Clive Adrian Smith , Xin Li , Frank F. Craig
CPC classification number: G01N15/1031 , B01L3/502784 , G01N15/1404 , B01L3/502715 , B01L3/50273 , G01N15/1056 , B01L2400/0424 , B01L2300/0864 , B01L2400/0427 , B01L2400/084 , G01N2015/1486 , G01N2015/149 , B01L2200/0652 , B01L2300/0627 , B01L2300/0816 , G01N2015/1081 , H01J49/0031
Abstract: A method for aligning sequences of droplets in streams of an emulsion comprising target droplets and tag droplets, a tag droplet comprising first and second tags. A target droplet is split into first and second target droplets and a tag droplet is split into first and second tag droplets. Each of the first and second tag droplets comprise the first and second tags. The first target droplet and first tag droplet are in a first stream of droplets, and the second target droplet and second tag droplet are in a second stream of droplets. The method detects the first tag droplets and first target droplets in the first stream and the second tag droplets and second target droplets in the second stream, determines a first sequence of droplets in the first stream and a second sequence of droplets in the second stream, and compares these to align the sequences.
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公开(公告)号：US20170227440A1
公开(公告)日：2017-08-10
申请号：US15502630
申请日：2015-08-07
Applicant: Sphere Fluidics Limited
Inventor： Clive Adrian Smith , Xin Li , Frank F. Craig
Abstract: We describe a method comprising: providing a droplet comprising a plurality of constituents, splitting said droplet into a first droplet and a second droplet, wherein said first droplet comprises a first fraction of said plurality of constituents and said second droplet comprises a second fraction of said plurality of constituents, analysing said constituents of said first fraction of said plurality of constituents in said first droplet, and sorting said second droplet dependent on an outcome of said analysis.
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