公开(公告)号：US20240417621A1

公开(公告)日：2024-12-19

申请号：US18818982

申请日：2024-08-29

Applicant: SPHERE FLUIDICS LIMITED

Inventor： Xin LI ,  Clive A. SMITH ,  Alexandra CLAY ,  Colin PILKINGTON

IPC: C09K23/18 ,  B01D11/04 ,  B01D17/04 ,  B01D17/12 ,  B01L3/00 ,  C08G65/00 ,  C09K23/00 ,  C09K23/16

Abstract: A surfactant of formula (I): A-(L1)a-(CH2)b-(L2)c-X  (I) wherein A is a perfluoropolyether; L1 is CONR′, wherein R′ is selected from H and C1-6 alkyl; a is 0 or 1; b is 0 or an integer between 1 and 10; L2 is a linking group; c is 0 or 1; and X is a charged group.

公开(公告)号：US20230158502A1

公开(公告)日：2023-05-25

申请号：US17919398

申请日：2020-04-17

Applicant: SPHERE FLUIDICS LIMITED

Inventor： Dimitris Josephides ,  James LONG ,  Xin LI

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L2200/0636 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2300/0867

Abstract: We describe a microfluidic structure for spacing out droplets, the structure comprising: a main channel for guiding droplets in a spacing fluid; a first inlet for introducing droplets into the main channel; and a second inlet for introducing a spacing fluid into the main channel, wherein a cross-sectional area of the main channel decreases downstream from the first inlet. We also describe a method of spacing out droplets using a microfluidic structure.

公开(公告)号：US20180133715A1

公开(公告)日：2018-05-17

申请号：US15578455

申请日：2016-06-03

Applicant: SPHERE FLUIDICS LIMITED

Inventor： Frank F. CRAIG ,  Marian REHAK ,  David HOLMES ,  Clive A. SMITH ,  Xin LIU ,  Giuseppe BENAZZI ,  Xin LI ,  Vinayaka PAWATE

IPC: B01L3/00 ,  G01N35/08 ,  G01N15/14

CPC classification number: B01L3/502784 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0627 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/0644 ,  G01N15/1484 ,  G01N35/08 ,  G01N2015/1481 ,  G01N2015/149

Abstract: Instruments and cartridges for processing droplets in emulsions containing biological entities such as cells. A method of such processing comprises providing a plurality of the entities in a fluid; preparing a droplet from the fluid; determining whether the droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of the determination; and dispensing the sorted droplet into a reservoir. The dispensing may comprise identifying and extracting the sorted droplet from a first fluidic flow path of said fluid by transferring the sorted droplet from into a second fluidic flow path and then ejecting the sorted droplet into a reservoir by applying pressure to the second fluidic flow path. The droplet contents may be tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and retrieved.

公开(公告)号：US20230193137A1

公开(公告)日：2023-06-22

申请号：US17922283

申请日：2021-05-04

Applicant: Sphere Fluidics Limited

Inventor： Xin LI ,  Clive Smith ,  Ben Mackworth

IPC: C09K23/42

CPC classification number: C09K23/42

Abstract: The present invention provides a surfactant of formula (I): (A)m-X-(B)n (I) wherein X is a linking group; each A is N independently a fluorocarbon or a perfluoropolyether; each B is independently (II) wherein a is an integer between 3 and 50 and each R is independently C1-6 alkyl, CH2CH2OC1-6 alkyl or CH2CH(CH3)OC1-6 alkyl; m is an integer between 1 and 10; and n is an integer between 1 and 10.

公开(公告)号：US20180250677A1

公开(公告)日：2018-09-06

申请号：US15754754

申请日：2016-09-06

Applicant: SPHERE FLUIDICS LIMITED

Inventor： Xin LI ,  Xin Liu ,  Alexandra Clay

IPC: B01L3/00 ,  B01L3/02

CPC classification number: B01L3/502776 ,  B01L3/0241 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/161 ,  B01L2400/0463 ,  B01L2400/0487

Abstract: A microfluidic structure for spacing out and aligning entities in an aqueous suspension is provided. The structure comprises: a channel for guiding entities in an aqueous suspension; a first comb of first inlets arranged on a first side of the channel for introducing a spacing medium into the channel; and a second comb of second inlets arranged on a second side of the channel for introducing the spacing medium into the channel; wherein the first side is opposite the second side, and wherein one of the first inlets has a corresponding, respective one of the second inlets at a substantially similar longitudinal position along the channel.

公开(公告)号：US20200316545A1

公开(公告)日：2020-10-08

申请号：US16768121

申请日：2018-11-29

Applicant: SPHERE FLUIDICS LIMITED

Inventor： Xin LI ,  Clive A. SMITH ,  Alexandra CLAY ,  Colin PILKINGTON

IPC: B01F17/00 ,  C08G65/00 ,  B01D17/04 ,  B01D17/12 ,  B01D11/04

Abstract: A surfactant of formula (I): A-(L1)a-(C2)b-(L2)c-X   (I) whereinA is a perfluoropolyether;L1 is CONR', wherein R′ is selected from H and C1-6 alkyl;a is 0 or 1;b is 0 or an integer between 1 and 10;L2 is a linking group;c is 0 or 1; andX is a charged group.

公开(公告)号：US20200017635A1

公开(公告)日：2020-01-16

申请号：US16303769

申请日：2017-05-26

Applicant: SPHERE FLUIDICS LIMITED

Inventor： Xin LI ,  Olive A Smith ,  Alexandra Clay ,  Frank F Craig

IPC: C08G65/332 ,  C08G65/00 ,  C08G65/333 ,  C08G65/334 ,  C12Q1/6806 ,  B01F17/00

Abstract: The present invention provides a surfactant having a formula selected from the group consisting of: B—((X)x—(CH2)a-Mn(VI), (A-(CH2)—(X)x—B—(X)x—(CH2)a-A)n (IV), (A-(CH2)a—(X)x—B)n (V), and (B)n—(X)x—(CH2)a—A (VII), wherein A is a perfluoropolyether; a is a positive integer; X is either a covalent bond or a linking group; x is a positive integer; B is a polyalkylene oxide unit; n is a positive integer greater than 1 and, in compounds comprising more than one A, B, X, a and x, each may be the same or different. The present invention also relates to methods of making such surfactants, uses of such surfactants and emulsions comprising such surfactants.

公开(公告)号：US20160252446A1

公开(公告)日：2016-09-01

申请号：US14907867

申请日：2014-07-30

Applicant: Sphere Fluidics Limited

Inventor： Clive A. SMITH ,  Xin LI ,  Karine Enesa ,  Xin LIU ,  Frank F. CRAIG ,  Graeme WHYTE

IPC: G01N15/14

CPC classification number: G01N15/1434 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2200/0673 ,  C12Q1/04 ,  C12Q1/06 ,  G01N15/1425 ,  G01N21/49 ,  G01N2015/0065 ,  G01N2015/149 ,  Y10T436/2575

Abstract: We describe a method of label-free cell and/or particle sorting in a microfluidic device, the method comprising; providing a stream of aqueous droplets in oil in a channel of said microfluidic device, wherein at least some of said droplets include cells and/or particles illuminating said stream from a first direction; detecting, for example, scattered light from cells and/or particles within said aqueous droplets in a second direction; determining, for example, a number of said cells and/or particles in each droplet from the scattered light; and sorting said aqueous droplets into one of a plurality of differentiated streams responsive to said determined number. The method further comprises modifying a refractive index of said oil to more closely match a refractive index of said aqueous droplets to reduce light scattered from boundaries of said droplets. Optionally a volume of the droplets is also controlled. In embodiments the oil is fluorous oil. We describe examples of preferred refractive index modifying compounds.

Abstract translation: 我们描述了在微流体装置中无标记细胞和/或颗粒分选的方法，该方法包括： 在所述微流体装置的通道中提供油中的水滴液滴，其中至少一些所述液滴包括从第一方向照射所述流的细胞和/或颗粒; 从第二方向检测来自所述水滴中的细胞和/或颗粒的散射光; 确定例如来自散射光的每个液滴中的所述细胞和/或颗粒的数量; 以及响应于所述确定的数量将所述水滴分选成多个分化流中的一个。 该方法还包括改变所述油的折射率以更紧密地匹配所述水滴的折射率，以减少从所述液滴的边界散射的光。 任选地，还控制一定体积的液滴。 在实施方案中，油是氟油。 我们描述优选折射率修饰化合物的实例。