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    Methodologies for the detection of human immunodeficiency virus nucleic acids employing oligonucleotide primer derived from the HIV-1 MVP5180/91 genome
    2.
    发明授权
    Methodologies for the detection of human immunodeficiency virus nucleic acids employing oligonucleotide primer derived from the HIV-1 MVP5180/91 genome 有权
    使用衍生自HIV-1 MVP5180 / 91基因组的寡核苷酸引物检测人类免疫缺陷病毒核酸的方法

    公开(公告)号：US06335158B2

    公开(公告)日：2002-01-01

    申请号：US09131551

    申请日：1998-08-10

    申请人： Stefan Brust ,  Stefan Knapp ,  Manfred Gerken ,  Lutz G. Guertler

    发明人： Stefan Brust ,  Stefan Knapp ,  Manfred Gerken ,  Lutz G. Guertler

    IPC分类号： C12Q170

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K16/1045 ,  C07K2317/21 ,  C12N2740/16022 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12Q1/703

    摘要： The present invention is directed toward nucleic-acid based methodologies for the detection of human immunodeficiency virus (HIV) nucleic acids in a sample. A novel HIV-1 isolate, designated MVP5180/91, was isolated from a West African Cameroonian patient with immunodeficiency. Nucleic acid and amino acid sequence comparisons of this isolate, with other HIV-1 strains of subtypes A-E and HIV-2 isolates, demonstrated that this virus shares only limited homology with other known HIV-1 and -2 isolates. However, this virus does display some genetic relatedness to another Cameroonian isolate designated ANT-70. These viruses form the basis for a new HIV-1 group which has been designated subtype O. An immunologically important epitope, corresponding to amino acids 601-623 of the MVP5180/91 transmembrane envelope glycoprotein, was identified. Labeled nucleic acids can be prepared from the nucleotide sequence encoding this region and employed in standard hybridization assays to detect HIV-1 nucleic acids. Alternatively, oligonucleotide primers can also be prepared from this region and employed in polymerase chain reaction (PCR) assays to detect viral-specific nucleic acids.

    摘要翻译： 本发明涉及用于检测样品中的人类免疫缺陷病毒（HIV）核酸的基于核酸的方法。 从具有免疫缺陷的西非喀麦隆患者中分离出一种名为MVP5180 / 91的新型HIV-1分离株。 该分离株与其他HIV-1亚型A-E和HIV-2分离株的核酸和氨基酸序列比较表明，该病毒与其他已知的HIV-1和-2分离株仅具有有限的同源性。 然而，这种病毒确实显示与另一个指定为ANT-70的喀麦隆分离株有一些遗传相关性。 这些病毒构成了已被指定为亚型O的新的HIV-1组的基础。鉴定了与MVP5180 / 91跨膜包膜糖蛋白的氨基酸601-623相当的免疫重要的表位。 标记的核酸可以从编码该区域的核苷酸序列制备，并用于标准杂交测定以检测HIV-1核酸。 或者，也可以从该区域制备寡核苷酸引物，并用于聚合酶链反应（PCR）测定中以检测病毒特异性核酸。

    Antigenic HIV gp41 chimeric polypeptides comprising the MVP5180/91 epitope SKGKLIS
    4.
    发明授权
    Antigenic HIV gp41 chimeric polypeptides comprising the MVP5180/91 epitope SKGKLIS 有权
    包含MVP5180 / 91表位SKGKLIS的抗原HIV gp41嵌合多肽

    公开(公告)号：US07803524B2

    公开(公告)日：2010-09-28

    申请号：US11084858

    申请日：2005-03-21

    申请人： Stefan Brust ,  Stefan Knapp ,  Manfred Gerken ,  Lutz G. Guertler

    发明人： Stefan Brust ,  Stefan Knapp ,  Manfred Gerken ,  Lutz G. Guertler

    IPC分类号： C12Q1/70 ,  A61K39/21 ,  B01L3/00

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K16/1045 ,  C07K2317/21 ,  C12N2740/16022 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12Q1/703

    摘要： Immunologically active peptides which are derived from a novel immunodeficiency virus which has the designation MVP5180/91 are described. A diagnostic composition containing such a peptide and methods of detecting an antibody against a retrovirus that causes immune deficiency using such diagnostic composition are also described. A kit containing the immunologically active peptides is also described. An immunogen and method of immunizing a mammal against HIV infection using the immunologically active peptides is described. DNA encoding the peptides and methods of detecting nucleic acids encoding HIV viruses are also described.

    摘要翻译： 描述了衍生自具有名称MVP5180 / 91的新型免疫缺陷病毒的免疫活性肽。 还描述了含有这种肽的诊断组合物和使用这种诊断组合物检测引起免疫缺陷的逆转录病毒抗体的方法。 还描述了含有免疫活性肽的试剂盒。 描述了使用免疫活性肽免疫哺乳动物抗HIV感染的免疫原和方法。 还描述了编码肽的DNA和检测编码HIV病毒的核酸的方法。

    Immunochemical determination of multivalent analytes
    10.
    发明授权
    Immunochemical determination of multivalent analytes 失效
    多价分析物的免疫化学测定

    公开(公告)号：US6120990A

    公开(公告)日：2000-09-19

    申请号：US90190

    申请日：1998-06-04

    申请人： Stefan Brust ,  Hans-Peter Hauser ,  Stefan Knapp ,  Helmut Peters

    发明人： Stefan Brust ,  Hans-Peter Hauser ,  Stefan Knapp ,  Helmut Peters

    IPC分类号： G01N33/53 ,  G01N33/531 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/70 ,  C12Q1/04

    CPC分类号： G01N33/6854 ,  G01N33/53 ,  G01N33/531 ,  G01N33/54306

    摘要： The present invention is directed to an immunochemical method for qualitatively or quantitatively detecting an analyte in which a first and a second binding component are contacted with the analyte, followed by detection of the binding of the binding components to the analyte. According to the invention, both of the binding components are prepared recombinantly in the same host using different vectors (V1 and V2) for expressing fusion proteins, with the first binding component being expressed as a fusion protein F1 in vector V1 and the second binding component being expressed as a fusion protein F2 in vector V2. The novel method, which can also be employed for simultaneously determining several analytes, is more sensitive and specific than prior known methods.

    摘要翻译： 本发明涉及用于定性或定量检测其中第一和第二结合组分与分析物接触的分析物的免疫化学方法，然后检测结合组分与分析物的结合。 根据本发明，使用不同的载体（V1和V2）在同一宿主中重组制备两种结合成分，用于表达融合蛋白，其中第一结合成分在载体V1中表达为融合蛋白F1，第二结合成分 在载体V2中表达为融合蛋白F2。 也可以用于同时测定多种分析物的新方法比现有已知的方法更敏感和特异。