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    Lipids and lipid assemblies comprising transfection enhancer elements
    1.
    发明申请
    Lipids and lipid assemblies comprising transfection enhancer elements 有权
    包含转染增强子元件的脂质和脂质组件

    公开(公告)号：US20080306153A1

    公开(公告)日：2008-12-11

    申请号：US12004154

    申请日：2007-12-19

    申请人： Steffen Panzner ,  Evgenios Siepi ,  Silke Lutz ,  Christian Reinsch ,  Claudia Muller

    发明人： Steffen Panzner ,  Evgenios Siepi ,  Silke Lutz ,  Christian Reinsch ,  Claudia Muller

    IPC分类号： A61K31/20 ,  C07C53/122 ,  C07C231/00

    CPC分类号： A61K9/1272 ,  A61K9/127 ,  A61K31/7052 ,  A61K47/542 ,  A61K47/544 ,  A61K47/545 ,  A61K47/554 ,  A61K47/6911 ,  A61K48/0008 ,  A61K48/0025 ,  A61K48/0033 ,  A61K48/0041 ,  C07C229/24 ,  C12N15/88 ,  Y02P20/582

    摘要： Lipid assemblies, such as liposomes, comprising transfection enhancer elements (TEE's), which are complexed with the lipid assemblies by means of ionic interactions, or lipids incorporating such TEE's are disclosed for enhancing the fusogenicity of the lipid assemblies. The TEE's have the formula: hydrophobic moiety-pH sensitive hydrophilic moiety  (II) The pH sensitive hydrophilic moiety of each TEE is a weak acid having a pka of between 2 and 6 or a zwitterionic structure comprising a combination of acidic groups with weak bases having a pKa of between 3 and 8. Lipids incorporating one or more such TEEs have the formula (I): Lipid moiety-[Hydrophobic moiety-pH sensitive hydrophilic moiety]  (I)

    摘要翻译： 公开了脂质体组合物，例如脂质体，其包含通过离子相互作用与脂质组合物复合的转染增强子元件（TEE）或掺入这种TEE的脂质，用于增强脂质组件的融合性。 TEE具有以下公式：<？in-line-formula description =“In-line Formulas”end =“lead”？>疏水部分 - pH敏感亲水部分（II）<？in-line-formula description =“In- 线型“end =”tail“？>每个TEE的pH敏感的亲水部分是pka为2至6的弱酸或包含具有pKa在3和3之间的弱碱基的酸性基团的组合的两性离子结构 包含一个或多个这样的TEE的脂质具有式（I）：<？在线公式描述=“在线公式”end =“lead”？>脂质部分 - [疏水部分 - pH敏感亲水部分] （I）<？in-line-formula description =“In-line Formulas”end =“tail”？>

    Efficient Method For Loading Amphoteric Liposomes With Nucleic Acid Active Substances
    4.
    发明申请
    Efficient Method For Loading Amphoteric Liposomes With Nucleic Acid Active Substances 审中-公开
    用核酸活性物质加载两性脂质体的有效方法

    公开(公告)号：US20120021042A1

    公开(公告)日：2012-01-26

    申请号：US12225030

    申请日：2007-03-16

    申请人： Steffen Panzner ,  Gerold Endert ,  Una Rauchhaus ,  Natalie Herzog ,  Claudia Muller

    发明人： Steffen Panzner ,  Gerold Endert ,  Una Rauchhaus ,  Natalie Herzog ,  Claudia Muller

    IPC分类号： A61K9/127 ,  A61K31/713 ,  A61K38/02 ,  A61K48/00 ,  C12N5/00

    CPC分类号： A61K9/1277 ,  A61K9/127 ,  A61K31/7088 ,  C12N15/88

    摘要： A method for preparing amphoteric liposomes loaded with polyanionic active agent as cargo, characterised by admixing an aqueous solution of said polyanionic active agent and an alcoholic solution of one or more amphiphiles and buffering said admixture to an acidic pH, said one or more amphiphiles being susceptible of forming amphoteric liposomes at said acidic pH, thereby to form such amphoteric liposomes in suspension encapsulating said active agent under conditions such that said liposomes form aggregates, and thereafter treating said suspension to dissociate said aggregates. Also disclosed are nucleic acid loaded amphoteric liposomes produced in accordance with the method, wherein said nucleic acids are oligonucleotides and said liposomes are multilamellar.

    摘要翻译： 一种制备负载聚阴离子活性剂作为货物的两性脂质体的方法，其特征在于将所述聚阴离子活性剂的水溶液与一种或多种两亲物的醇溶液混合并将所述混合物缓冲至酸性pH，所述一种或多种两亲物易感 在所述酸性pH下形成两性脂质体，从而在使所述脂质体形成聚集体的条件下形成包封所述活性剂的悬浮液的这种两性脂质体，然后处理所述悬浮液以离解所述聚集体。 还公开了根据该方法生产的核酸负载的两性脂质体，其中所述核酸是寡核苷酸，所述脂质体是多层的。

    Identification of antigen epitopes
    6.
    发明申请
    Identification of antigen epitopes 审中-公开
    抗原表位的鉴定

    公开(公告)号：US20070154953A1

    公开(公告)日：2007-07-05

    申请号：US10547533

    申请日：2004-03-03

    申请人： Herwig Brunner ,  Gunter Tover ,  Thomas Schiestel ,  Claudia Muller ,  Thomas Flad

    发明人： Herwig Brunner ,  Gunter Tover ,  Thomas Schiestel ,  Claudia Muller ,  Thomas Flad

    IPC分类号： G01N33/567 ,  G01N33/555 ,  G01N33/53

    CPC分类号： G01N33/543 ,  G01N33/6893

    摘要： The present invention relates to methods for identifying and/or detecting T-cell epitopes of a protein antigen, to methods for preparing peptide vaccines against a protein antigen, to methods for controlling the quality of receptor/ligand complexes and/or their components, to methods for preparing nanoparticles having at least one immobilized receptor unit or an immobilized receptor, to methods for preparing nanoparticles having immobilized receptor/ligand complexes, in particular peptide-presenting MHC molecules, to methods for enriching and/or isolating specific CD4+-T- or CD8+-T-lymphocytes from peripheral blood mononuclear cells, to methods for priming a CD8+-T-lymphocyte reaction in vitro, to nanoparticles having an immobilized receptor unit, in particular an immobilized chain of an MHC molecule, to nanoparticles having an immobilized receptor, in particular an immobilized MHC molecule, to nanoparticles having an immobilized receptor/ligand complex, in particular a peptide-presenting MHC molecule, to a peptide vaccine, to a kit for identifying and/or detecting T-cell epitopes of a protein antigen, and to the use of the nanoparticles for identifying and/or detecting T-cell epitopes, for preparing peptide vaccines, for enriching and/or isolating specific T-lymphocytes and for priming a CD8+-T-lymphocyte reaction in vitro.

    摘要翻译： 本发明涉及用于鉴定和/或检测蛋白质抗原的T细胞表位的方法，用于制备针对蛋白质抗原的肽疫苗的方法，以及用于控制受体/配体复合物和/或其组分的质量的方法， 用于制备具有至少一个固定化受体单元或固定化受体的纳米颗粒的方法，制备具有固定化受体/配体复合物，特别是肽呈递MHC分子的纳米颗粒的方法，用于富集和/或分离特异性CD4 + 来自外周血单核细胞的 -T-或CD8 + T淋巴细胞，以及用于在体外引发CD8 + T淋巴细胞反应的方法， 具有固定化受体单元，特别是MHC分子的固定化链的纳米颗粒具有固定化受体特别是固定的MHC分子的纳米颗粒具有固定的受体/配体复合物的纳米颗粒 ，特别是提供肽的MHC分子，用于肽疫苗，用于鉴定和/或检测蛋白质抗原的T细胞表位的试剂盒，以及使用纳米颗粒鉴定和/或检测T细胞表位 用于制备肽疫苗，用于富集和/或分离特异性T淋巴细胞和用于在体外引发CD8 + T淋巴细胞反应。