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    Production of polyunsaturated fatty acids in oleaginous yeasts
    3.
    发明申请
    Production of polyunsaturated fatty acids in oleaginous yeasts 有权
    在含油酵母中生产多不饱和脂肪酸

    公开(公告)号：US20050136519A1

    公开(公告)日：2005-06-23

    申请号：US10840579

    申请日：2004-05-06

    申请人： Stephen Picataggio ,  Narendra Yadav ,  Quinn Zhu

    发明人： Stephen Picataggio ,  Narendra Yadav ,  Quinn Zhu

    IPC分类号： C12N20060101 ,  C12N1/18 ,  C12N9/04 ,  C12N15/74 ,  C12P7/64

    CPC分类号： C12N15/815 ,  C12P7/6427 ,  C12P7/6472

    摘要： The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of Yarrowia for synthesis of ARA and EPA.

    摘要翻译： 本发明涉及在含油酵母中生产ω-3和/或ω-6脂肪酸的方法。 因此，能够催化亚油酸（LA）转化为γ-亚麻酸（GLA）的去饱和酶和延伸酶; α-亚油酸（ALA）至十八碳四烯酸（STA）; GLA至二氢-γ-亚油酸（DGLA）; STA至二十碳四烯酸（ETA）; DGLA对花生四烯酸（ARA）; ETA至二十碳五烯酸（EPA）; DGLA到ETA; EPA至二十二碳五烯酸（DPA）; 并且ARA至EPA已被引入耶氏酵母的基因组合成ARA和EPA。

    Production of polyunsaturated fatty acids in oleaginous yeasts
    5.
    发明申请
    Production of polyunsaturated fatty acids in oleaginous yeasts 有权
    在含油酵母中生产多不饱和脂肪酸

    公开(公告)号：US20070207528A1

    公开(公告)日：2007-09-06

    申请号：US11714377

    申请日：2007-03-05

    申请人： Stephen Picataggio ,  Narendra Yadav ,  Quinn Zhu

    发明人： Stephen Picataggio ,  Narendra Yadav ,  Quinn Zhu

    IPC分类号： C12P7/64 ,  C12N9/04 ,  C12N1/18 ,  C12N15/74

    CPC分类号： C12N15/815 ,  C12P7/6427 ,  C12P7/6472

    摘要： The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of Yarrowia for synthesis of ARA and EPA.

    摘要翻译： 本发明涉及在含油酵母中生产ω-3和/或ω-6脂肪酸的方法。 因此，能够催化亚油酸（LA）转化为γ-亚麻酸（GLA）的去饱和酶和延伸酶; α-亚油酸（ALA）至十八碳四烯酸（STA）; GLA至二氢-γ-亚油酸（DGLA）; STA至二十碳四烯酸（ETA）; DGLA对花生四烯酸（ARA）; ETA至二十碳五烯酸（EPA）; DGLA到ETA; EPA至二十二碳五烯酸（DPA）; 并且ARA至EPA已被引入耶氏酵母的基因组合成ARA和EPA。

    Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms
    6.
    发明申请
    Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms 失效
    酰基转移酶调节以增加含油生物的总脂质和油中多不饱和脂肪酸的百分比

    公开(公告)号：US20060094088A1

    公开(公告)日：2006-05-04

    申请号：US11190750

    申请日：2005-07-27

    申请人： Stephen Picataggio ,  Narendra Yadav ,  Hongxiang Zhang

    发明人： Stephen Picataggio ,  Narendra Yadav ,  Hongxiang Zhang

    IPC分类号： C12P7/64 ,  C07H21/04 ,  C12N9/10 ,  C12N15/74 ,  C12N1/18

    CPC分类号： C12N9/1029 ,  C12N15/8247 ,  C12P7/6472

    摘要： Methods to increase the percent of polyunsaturated fatty acids (PUFAs) within the total lipids and oils of PUFA-producing oleaginous organisms are provided herein, by regulating the activity of specific acyltransferases. Specifically, since oil biosynthesis is expected to compete with polyunsaturation during oleaginy, it is possible to reduce or inactivate the activity of an organism's DAG ATs (e.g., phospholipid:diacylglycerol acyltransferase (PDAT) and/or diacylglycerol acyltransferase 1 (DGAT1) and/or diacylglycerol acyltransferase 2 (DGAT2)) to thereby reduce the overall rate of oil biosynthesis while concomitantly increasing the percent of PUFAs that are incorporated into the lipid and oil fractions. The teachings herein will thereby enable one to engineer a wide variety of oleaginous organisms to produce oils with very specific fatty acid compositions.

    摘要翻译： 本文通过调节特异性酰基转移酶的活性来提高增加多不饱和脂肪酸（PUFA）在产生PUFA的含油生物体的总脂质和油中的百分数。 具体地说，由于油生物合成预期在含油期间与多不饱和度竞争，有可能减少或失活生物体的DAG AT（例如磷脂：二酰基甘油酰基转移酶（PDAT）和/或二酰基甘油酰基转移酶1（DGAT1））和/或 二酰基甘油酰基转移酶2（DGAT2）），从而降低油的合成速率，同时增加掺入脂质和油馏分中的PUFA的百分比。 因此，本文的教导使得能够设计各种含油生物以产生具有非常特定的脂肪酸组成的油。

    Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
    9.
    发明申请
    Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts 失效
    用于在含油酵母中生产多不饱和脂肪酸的密码子优化基因

    公开(公告)号：US20070004016A1

    公开(公告)日：2007-01-04

    申请号：US11499266

    申请日：2006-08-04

    申请人： Stephen Picataggio ,  Quinn Zhu

    发明人： Stephen Picataggio ,  Quinn Zhu

    IPC分类号： C12P7/64 ,  C12N9/04 ,  C07H21/04 ,  C12N15/74 ,  C12N1/18

    CPC分类号： C12N9/0071 ,  C12N9/1029

    摘要： The present invention relates to fatty acid desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to ETA; eicosapentaenoic acid (EPA) to docosapentaenoic acid (DPA); and arachidonic acid (ARA) to EPA. Nucleic acid sequences encoding codon-optimized desaturases and elongases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the codon-optimized desaturase or elongases, and recombinant host microorganisms expressing increased levels of desaturase or elongase are described.

    摘要翻译： 本发明涉及能够催化亚油酸（LA）转化为γ-亚麻酸（GLA）的脂肪酸去饱和酶和延伸酶; α-亚油酸（ALA）至十八碳四烯酸（STA）; GLA至二氢-γ-亚油酸（DGLA）; STA至二十碳四烯酸（ETA）; DGLA到ETA; 二十碳五烯酸（EPA）至二十二碳五烯酸（DPA）; 和花生四烯酸（ARA）。 描述了编码密码​​子优化的去饱和酶和延伸酶的核酸序列，与其杂交的核酸序列，包含密码子优化的去饱和酶或延伸酶的DNA构建体，以及表达增加的去饱和酶或延长酶水平的重组宿主微生物。